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Protein

Aldo-keto reductase family 1 member C18

Gene

Akr1c18

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of progesterone into 20-alpha-dihydroprogesterone (20 alpha-OHP).

Catalytic activityi

17-alpha,20-alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17-alpha-hydroxyprogesterone + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei50 – 501NADPBy similarity
Sitei54 – 541Important for substrate specificityBy similarity
Active sitei55 – 551Proton donorBy similarity
Sitei84 – 841Lowers pKa of active site TyrBy similarity
Binding sitei117 – 1171SubstrateBy similarity
Binding sitei190 – 1901NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi20 – 245NADPBy similarity
Nucleotide bindingi166 – 1672NADPBy similarity
Nucleotide bindingi216 – 2216NADPBy similarity
Nucleotide bindingi270 – 28011NADPBy similarityAdd
BLAST

GO - Molecular functioni

  • 17-alpha,20-alpha-dihydroxypregn-4-en-3-one dehydrogenase activity Source: RGD
  • alditol:NADP+ 1-oxidoreductase activity Source: RGD
  • aldo-keto reductase (NADP) activity Source: RGD

GO - Biological processi

  • cellular response to follicle-stimulating hormone stimulus Source: RGD
  • cellular response to forskolin Source: RGD
  • cellular response to gonadotropin-releasing hormone Source: RGD
  • cellular response to prolactin Source: RGD
  • cellular response to testosterone stimulus Source: RGD
  • cellular response to transforming growth factor beta stimulus Source: RGD
  • female pregnancy Source: RGD
  • parturition Source: Ensembl
  • progesterone catabolic process Source: Ensembl
  • progesterone metabolic process Source: RGD
  • regulation of steroid biosynthetic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-RNO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-RNO-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-RNO-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-RNO-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
R-RNO-5365859. RA biosynthesis pathway.
R-RNO-975634. Retinoid metabolism and transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Aldo-keto reductase family 1 member C18 (EC:1.1.-.-)
Alternative name(s):
20-alpha-hydroxysteroid dehydrogenase (EC:1.1.1.149)
Short name:
20-alpha-HSD
HSD1
Gene namesi
Name:Akr1c18
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 17

Organism-specific databases

RGDi708428. Akr1c18.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: MGI
  • nucleus Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 323323Aldo-keto reductase family 1 member C18PRO_0000124650Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP51652.
PRIDEiP51652.

Expressioni

Tissue specificityi

Corpus luteum (large luteal cells).

Developmental stagei

Remains repressed throughout pregnancy and becomes highly and rapidly expressed before parturition.

Inductioni

Repressed by prolactin.

Gene expression databases

BgeeiENSRNOG00000017531.
GenevisibleiP51652. RN.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023739.

Structurei

3D structure databases

ProteinModelPortaliP51652.
SMRiP51652. Positions 6-323.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOVERGENiHBG000020.
InParanoidiP51652.
KOiK05295.
OMAiCINEDTP.
OrthoDBiEOG091G0D69.
PhylomeDBiP51652.
TreeFamiTF106492.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51652-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSKIQKMEL NDGHSIPVLG FGTYATEENL RKKSMESTKI AIDVGFRHID
60 70 80 90 100
CSHLYQNEEE IGQAIVSKIE DGTVKREDIF YTSKLWSTSH RPELVRPSLE
110 120 130 140 150
NSLRKLNLDY VDLYLIHFPV SLKPGDELLP QDEHGNLILD TVDLCDTWEA
160 170 180 190 200
MEKCKDAGLA KSIGVSNFNR RQLEKILNKP GLKHRPVCNQ VECHLYLNQS
210 220 230 240 250
KLLAYCKMND IVLVAYGALG TQRYKYCINE DTPVLLDDPI LCTMAKKYKR
260 270 280 290 300
TPALIALRYQ LERGIVTLVK SFNEERIREN LQVFDFQLAS DDMEILDNLD
310 320
RNLRYFPANM FKAHPNFPFS DEY
Length:323
Mass (Da):37,300
Last modified:October 1, 1996 - v1
Checksum:iD14270961515195E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti242 – 2421C → L AA sequence (PubMed:8194472).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32601 mRNA. Translation: AAA40601.1.
D14424 mRNA. Translation: BAA03317.1.
BC100248 mRNA. Translation: AAI00249.1.
PIRiJC2330.
RefSeqiNP_612519.1. NM_138510.1.
UniGeneiRn.10030.

Genome annotation databases

EnsembliENSRNOT00000023739; ENSRNOP00000023739; ENSRNOG00000017531.
GeneIDi171516.
KEGGirno:171516.
UCSCiRGD:708428. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L32601 mRNA. Translation: AAA40601.1.
D14424 mRNA. Translation: BAA03317.1.
BC100248 mRNA. Translation: AAI00249.1.
PIRiJC2330.
RefSeqiNP_612519.1. NM_138510.1.
UniGeneiRn.10030.

3D structure databases

ProteinModelPortaliP51652.
SMRiP51652. Positions 6-323.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023739.

Proteomic databases

PaxDbiP51652.
PRIDEiP51652.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023739; ENSRNOP00000023739; ENSRNOG00000017531.
GeneIDi171516.
KEGGirno:171516.
UCSCiRGD:708428. rat.

Organism-specific databases

CTDi8644.
RGDi708428. Akr1c18.

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
GeneTreeiENSGT00760000119041.
HOVERGENiHBG000020.
InParanoidiP51652.
KOiK05295.
OMAiCINEDTP.
OrthoDBiEOG091G0D69.
PhylomeDBiP51652.
TreeFamiTF106492.

Enzyme and pathway databases

ReactomeiR-RNO-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-RNO-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
R-RNO-193807. Synthesis of bile acids and bile salts via 27-hydroxycholesterol.
R-RNO-2162123. Synthesis of Prostaglandins (PG) and Thromboxanes (TX).
R-RNO-5365859. RA biosynthesis pathway.
R-RNO-975634. Retinoid metabolism and transport.

Miscellaneous databases

PROiP51652.

Gene expression databases

BgeeiENSRNOG00000017531.
GenevisibleiP51652. RN.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAKC1H_RAT
AccessioniPrimary (citable) accession number: P51652
Secondary accession number(s): Q498E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.