Reviewed, UniProtKB/Swiss-Prot P51652 (AKC1H_RAT)

Names and origin

Protein names

Recommended name:
    Aldo-keto reductase family 1 member C18
    EC=1.1.-.-
Alternative name(s):
    20-alpha-hydroxysteroid dehydrogenase
      Short name=20-alpha-HSD
    EC=1.1.1.149
    HSD1

Gene names

Name:

Akr1c18

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of progesterone into 20-alpha-dihydroprogesterone (20 alpha-OHP).
Catalytic activity	17-alpha,20-alpha-dihydroxypregn-4-en-3-one + NAD(P)+ = 17-alpha-hydroxyprogesterone + NAD(P)H.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Tissue specificity	Corpus luteum (large luteal cells).
Developmental stage	Remains repressed throughout pregnancy and becomes highly and rapidly expressed before parturition.
Induction	Repressed by prolactin.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW progesterone metabolic process Ref.2 Inferred from direct assay. Source: MGI regulation of steroid biosynthetic process Inferred from direct assay. Source: RGD
Cellular component	cytosol Ref.4 Inferred from direct assay. Source: MGI soluble fraction Ref.4 Inferred from direct assay. Source: MGI
Molecular function	20-alpha-hydroxysteroid dehydrogenase activity Ref.2 Inferred from direct assay. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 323	323	Aldo-keto reductase family 1 member C18		PRO_0000124650
Regions
	Nucleotide binding	13 – 22	10	NADP Potential
	Nucleotide binding	217 – 280	64	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	117	1	Substrate By similarity
	Site	54	1	Important for substrate specificity By similarity
	Site	84	1	Lowers pKa of active site Tyr By similarity
Experimental info
	Sequence conflict	242	1	C → L AA sequence Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P51652-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: D14270961515195E	FASTA	323	37,300
	10 20 30 40 50 60 MNSKIQKMEL NDGHSIPVLG FGTYATEENL RKKSMESTKI AIDVGFRHID CSHLYQNEEE 70 80 90 100 110 120 IGQAIVSKIE DGTVKREDIF YTSKLWSTSH RPELVRPSLE NSLRKLNLDY VDLYLIHFPV 130 140 150 160 170 180 SLKPGDELLP QDEHGNLILD TVDLCDTWEA MEKCKDAGLA KSIGVSNFNR RQLEKILNKP 190 200 210 220 230 240 GLKHRPVCNQ VECHLYLNQS KLLAYCKMND IVLVAYGALG TQRYKYCINE DTPVLLDDPI 250 260 270 280 290 300 LCTMAKKYKR TPALIALRYQ LERGIVTLVK SFNEERIREN LQVFDFQLAS DDMEILDNLD 310 320 RNLRYFPANM FKAHPNFPFS DEY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation and characterization of a rat luteal cDNA encoding 20 alpha-hydroxysteroid dehydrogenase." Mao J., Duan W.R., Albarracin C.T., Parmer T.G., Gibori G. Biochem. Biophys. Res. Commun. 201:1289-1295(1994) [PubMed: 8024573] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 257-269 AND 315-323. Strain: Sprague-Dawley. Tissue: Corpus luteum.
[2]	"Molecular cloning of cDNA for rat ovarian 20 alpha-hydroxysteroid dehydrogenase (HSD1)." Miura R., Shiota K., Noda K., Yagi S., Ogawa T., Takahashi M. Biochem. J. 299:561-567(1994) [PubMed: 8172618] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar Imamichi. Tissue: Ovary.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[4]	"Identification of a major prolactin-regulated protein as 20 alpha-hydroxysteroid dehydrogenase: coordinate regulation of its activity, protein content, and messenger ribonucleic acid expression." Albarracin C.T., Parmer T.G., Duan W.R., Nelson S.E., Gibori G. Endocrinology 134:2453-2460(1994) [PubMed: 8194472] [Abstract] Cited for: PROTEIN SEQUENCE OF 233-243; 252-269 AND 315-323. Strain: Sprague-Dawley. Tissue: Corpus luteum.
[5]	Noda K., Shiota K., Ogawa T., Yagi S., Takahashi M. J. Reprod. Dev. 39:169-173(1993) Cited for: PROTEIN SEQUENCE OF 3-21.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L32601 mRNA. Translation: AAA40601.1. D14424 mRNA. Translation: BAA03317.1. BC100248 mRNA. Translation: AAI00249.1.
IPI	IPI00204302.
PIR	JC2330.
RefSeq	NP_612519.1.
UniGene	Rn.10030
3D structure databases
HSSP	HSSP built from PDB template 1LWI based on UniProtKB P23457.
SMR	P51652. Positions 6-323.
ModBase	Search...
Proteomic databases
PRIDE	P51652.
Genome annotation databases
Ensembl	ENSRNOG00000017531. Rattus norvegicus. [Contig view]
GeneID	171516.
KEGG	rno:171516.
Organism-specific databases
RGD	708428. LOC171516.
Phylogenomic databases
HOVERGEN	P51652.
OMA	P51652. GTQRYKY.
Enzyme and pathway databases
BRENDA	1.1.1.149. 248.
Gene expression databases
ArrayExpress	P51652.
GermOnline	ENSRNOG00000017531. Rattus norvegicus.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	622481.

Entry information

Entry name

AKC1H_RAT

Accession

Primary (citable) accession number: P51652
Secondary accession number(s): Q498E4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)