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Protein

Succinate-semialdehyde dehydrogenase, mitochondrial

Gene

Aldh5a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).By similarity

Catalytic activityi

Succinate semialdehyde + NAD+ + H2O = succinate + NADH.

Enzyme regulationi

Redox-regulated. Inhibited under oxydizing conditions (By similarity).By similarity

Pathwayi: 4-aminobutanoate degradation

This protein is involved in the pathway 4-aminobutanoate degradation, which is part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the pathway 4-aminobutanoate degradation and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei193Transition state stabilizerBy similarity1
Binding sitei201NADBy similarity1
Binding sitei201SubstrateBy similarity1
Active sitei294Proton acceptorPROSITE-ProRule annotation1
Binding sitei322SubstrateBy similarity1
Active sitei328NucleophilePROSITE-ProRule annotation1
Binding sitei486SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi216 – 219NADBy similarity4
Nucleotide bindingi272 – 277NADBy similarity6

GO - Molecular functioni

  • aldehyde dehydrogenase (NAD) activity Source: GO_Central
  • carboxylic acid binding Source: RGD
  • NAD binding Source: RGD
  • succinate-semialdehyde dehydrogenase (NAD+) activity Source: UniProtKB
  • succinate-semialdehyde dehydrogenase [NAD(P)+] activity Source: GO_Central

GO - Biological processi

  • central nervous system development Source: UniProtKB
  • gamma-aminobutyric acid catabolic process Source: UniProtKB
  • oxidation-reduction process Source: UniProtKB
  • succinate metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

BRENDAi1.2.1.3. 5301.
SABIO-RKP51650.
UniPathwayiUPA00733.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate-semialdehyde dehydrogenase, mitochondrial (EC:1.2.1.24)
Alternative name(s):
Aldehyde dehydrogenase family 5 member A1
NAD(+)-dependent succinic semialdehyde dehydrogenase
Gene namesi
Name:Aldh5a1
Synonyms:Ssadh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621422. Aldh5a1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 35Mitochondrion2 PublicationsAdd BLAST35
ChainiPRO_000005656736 – 523Succinate-semialdehyde dehydrogenase, mitochondrialAdd BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei114N6-acetyllysine; alternateBy similarity1
Modified residuei114N6-succinyllysine; alternateBy similarity1
Modified residuei123N6-succinyllysineBy similarity1
Modified residuei172N6-succinyllysineBy similarity1
Modified residuei253N6-acetyllysine; alternateBy similarity1
Modified residuei253N6-succinyllysine; alternateBy similarity1
Disulfide bondi328 ↔ 330In inhibited formBy similarity
Modified residuei353N6-acetyllysineBy similarity1
Modified residuei390N6-succinyllysineBy similarity1
Modified residuei399N6-acetyllysineBy similarity1
Modified residuei487PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP51650.
PeptideAtlasiP51650.
PRIDEiP51650.

2D gel databases

World-2DPAGE0004:P51650.

PTM databases

iPTMnetiP51650.
PhosphoSitePlusiP51650.

Expressioni

Tissue specificityi

Brain, pancreas, heart, liver, skeletal muscle, kidney. Lower in spleen, lung, kidney and testis.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035601.

Structurei

3D structure databases

ProteinModelPortaliP51650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2451. Eukaryota.
COG1012. LUCA.
HOGENOMiHOG000271509.
HOVERGENiHBG108515.
InParanoidiP51650.
PhylomeDBiP51650.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010102. Succ_semiAld_DH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01780. SSADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Long (identifier: P51650-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATCFLLRNF CAARPALRPP GRLLREPAGA QRRSYVGGPA DLHADLLRGD
60 70 80 90 100
SFVGGRWLPT PATFPVYDPA SGAKLGTVAD CGVPEARAAV RAAYDAFSSW
110 120 130 140 150
KEISVKERSS LLRKWYDLMI QNKDELAKII TAESGKPLKE AQGEILYSAF
160 170 180 190 200
FLEWFSEEAR RVYGDIIYTS AKDKRGLVLK QPVGVASIIT PWNFPSAMIT
210 220 230 240 250
RKVGAALAAG CTVVVKPAED TPYSALALAQ LANQAGIPPG VYNVIPCSRT
260 270 280 290 300
KAKEVGEVLC TDPLVSKISF TGSTATGKIL LHHAANSVKR VSMELGGLAP
310 320 330 340 350
FIVFDSANVD QAVAGAMASK FRNAGQTCVC SNRFLVQRGI HDSFVTKFAE
360 370 380 390 400
AMKKSLRVGN GFEEGTTQGP LINEKAVEKV EKHVNDAVAK GATVVTGGKR
410 420 430 440 450
HQSGGNFFEP TLLSNVTRDM LCITEETFGP VAPVIKFDKE EEAVAIANAA
460 470 480 490 500
DVGLAGYFYS QDPAQIWRVA EQLEVGMVGV NEGLISSVEC PFGGVKQSGL
510 520
GREGSKYGID EYLEVKYVCY GGL
Length:523
Mass (Da):56,131
Last modified:April 8, 2008 - v2
Checksum:i4CA521139C9FA98F
GO
Isoform Short (identifier: P51650-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     107-134: Missing.

Note: No experimental confirmation available.
Show »
Length:495
Mass (Da):52,798
Checksum:iFC69A1D9215CEF5F
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_001284107 – 134Missing in isoform Short. 1 PublicationAdd BLAST28

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03105019 Genomic DNA. No translation available.
L34821 mRNA. Translation: AAA67058.1.
PIRiI61704.
UniGeneiRn.10070.

Genome annotation databases

UCSCiRGD:621422. rat. [P51650-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03105019 Genomic DNA. No translation available.
L34821 mRNA. Translation: AAA67058.1.
PIRiI61704.
UniGeneiRn.10070.

3D structure databases

ProteinModelPortaliP51650.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035601.

PTM databases

iPTMnetiP51650.
PhosphoSitePlusiP51650.

2D gel databases

World-2DPAGE0004:P51650.

Proteomic databases

PaxDbiP51650.
PeptideAtlasiP51650.
PRIDEiP51650.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:621422. rat. [P51650-1]

Organism-specific databases

RGDi621422. Aldh5a1.

Phylogenomic databases

eggNOGiKOG2451. Eukaryota.
COG1012. LUCA.
HOGENOMiHOG000271509.
HOVERGENiHBG108515.
InParanoidiP51650.
PhylomeDBiP51650.

Enzyme and pathway databases

UniPathwayiUPA00733.
BRENDAi1.2.1.3. 5301.
SABIO-RKP51650.

Miscellaneous databases

PROiP51650.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010102. Succ_semiAld_DH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01780. SSADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSSDH_RAT
AccessioniPrimary (citable) accession number: P51650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 8, 2008
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.