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P51650

- SSDH_RAT

UniProt

P51650 - SSDH_RAT

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Protein

Succinate-semialdehyde dehydrogenase, mitochondrial

Gene

Aldh5a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes one step in the degradation of the inhibitory neurotransmitter gamma-aminobutyric acid (GABA).By similarity

Catalytic activityi

Succinate semialdehyde + NAD+ + H2O = succinate + NADH.

Enzyme regulationi

Redox-regulated. Inhibited under oxydizing conditions (By similarity).By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei193 – 1931Transition state stabilizerBy similarity
Binding sitei201 – 2011NADBy similarity
Binding sitei201 – 2011SubstrateBy similarity
Active sitei294 – 2941Proton acceptorPROSITE-ProRule annotation
Binding sitei322 – 3221SubstrateBy similarity
Active sitei328 – 3281NucleophilePROSITE-ProRule annotation
Binding sitei486 – 4861SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi216 – 2194NADBy similarity
Nucleotide bindingi272 – 2776NADBy similarity

GO - Molecular functioni

  1. carboxylic acid binding Source: RGD
  2. NAD binding Source: RGD
  3. succinate-semialdehyde dehydrogenase (NAD+) activity Source: UniProtKB
  4. succinate-semialdehyde dehydrogenase [NAD(P)+] activity Source: InterPro

GO - Biological processi

  1. central nervous system development Source: UniProtKB
  2. gamma-aminobutyric acid catabolic process Source: UniProtKB
  3. oxidation-reduction process Source: UniProtKB
  4. succinate metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKP51650.
UniPathwayiUPA00733.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate-semialdehyde dehydrogenase, mitochondrial (EC:1.2.1.24)
Alternative name(s):
Aldehyde dehydrogenase family 5 member A1
NAD(+)-dependent succinic semialdehyde dehydrogenase
Gene namesi
Name:Aldh5a1
Synonyms:Ssadh
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi621422. Aldh5a1.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3535Mitochondrion2 PublicationsAdd
BLAST
Chaini36 – 523488Succinate-semialdehyde dehydrogenase, mitochondrialPRO_0000056567Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei114 – 1141N6-acetyllysine; alternateBy similarity
Modified residuei114 – 1141N6-succinyllysine; alternateBy similarity
Modified residuei123 – 1231N6-succinyllysineBy similarity
Modified residuei172 – 1721N6-succinyllysineBy similarity
Modified residuei253 – 2531N6-acetyllysine; alternateBy similarity
Modified residuei253 – 2531N6-succinyllysine; alternateBy similarity
Disulfide bondi328 ↔ 330In inhibited formBy similarity
Modified residuei353 – 3531N6-acetyllysineBy similarity
Modified residuei390 – 3901N6-succinyllysineBy similarity
Modified residuei399 – 3991N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond

Proteomic databases

PaxDbiP51650.
PRIDEiP51650.

2D gel databases

World-2DPAGE0004:P51650.

PTM databases

PhosphoSiteiP51650.

Expressioni

Tissue specificityi

Brain, pancreas, heart, liver, skeletal muscle, kidney. Lower in spleen, lung, kidney and testis.

Gene expression databases

GenevestigatoriP51650.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000035601.

Structurei

3D structure databases

ProteinModelPortaliP51650.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldehyde dehydrogenase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG1012.
HOGENOMiHOG000271509.
HOVERGENiHBG108515.
InParanoidiP51650.
PhylomeDBiP51650.

Family and domain databases

Gene3Di3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProiIPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010102. Succ_semiAld_DH.
[Graphical view]
PfamiPF00171. Aldedh. 1 hit.
[Graphical view]
SUPFAMiSSF53720. SSF53720. 1 hit.
TIGRFAMsiTIGR01780. SSADH. 1 hit.
PROSITEiPS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Long (identifier: P51650-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATCFLLRNF CAARPALRPP GRLLREPAGA QRRSYVGGPA DLHADLLRGD
60 70 80 90 100
SFVGGRWLPT PATFPVYDPA SGAKLGTVAD CGVPEARAAV RAAYDAFSSW
110 120 130 140 150
KEISVKERSS LLRKWYDLMI QNKDELAKII TAESGKPLKE AQGEILYSAF
160 170 180 190 200
FLEWFSEEAR RVYGDIIYTS AKDKRGLVLK QPVGVASIIT PWNFPSAMIT
210 220 230 240 250
RKVGAALAAG CTVVVKPAED TPYSALALAQ LANQAGIPPG VYNVIPCSRT
260 270 280 290 300
KAKEVGEVLC TDPLVSKISF TGSTATGKIL LHHAANSVKR VSMELGGLAP
310 320 330 340 350
FIVFDSANVD QAVAGAMASK FRNAGQTCVC SNRFLVQRGI HDSFVTKFAE
360 370 380 390 400
AMKKSLRVGN GFEEGTTQGP LINEKAVEKV EKHVNDAVAK GATVVTGGKR
410 420 430 440 450
HQSGGNFFEP TLLSNVTRDM LCITEETFGP VAPVIKFDKE EEAVAIANAA
460 470 480 490 500
DVGLAGYFYS QDPAQIWRVA EQLEVGMVGV NEGLISSVEC PFGGVKQSGL
510 520
GREGSKYGID EYLEVKYVCY GGL
Length:523
Mass (Da):56,131
Last modified:April 8, 2008 - v2
Checksum:i4CA521139C9FA98F
GO
Isoform Short (identifier: P51650-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     107-134: Missing.

Note: No experimental confirmation available.

Show »
Length:495
Mass (Da):52,798
Checksum:iFC69A1D9215CEF5F
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei107 – 13428Missing in isoform Short. 1 PublicationVSP_001284Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AABR03105019 Genomic DNA. No translation available.
L34821 mRNA. Translation: AAA67058.1.
PIRiI61704.
UniGeneiRn.10070.

Genome annotation databases

UCSCiRGD:621422. rat. [P51650-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AABR03105019 Genomic DNA. No translation available.
L34821 mRNA. Translation: AAA67058.1 .
PIRi I61704.
UniGenei Rn.10070.

3D structure databases

ProteinModelPortali P51650.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000035601.

PTM databases

PhosphoSitei P51650.

2D gel databases

World-2DPAGE 0004:P51650.

Proteomic databases

PaxDbi P51650.
PRIDEi P51650.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:621422. rat. [P51650-1 ]

Organism-specific databases

RGDi 621422. Aldh5a1.

Phylogenomic databases

eggNOGi COG1012.
HOGENOMi HOG000271509.
HOVERGENi HBG108515.
InParanoidi P51650.
PhylomeDBi P51650.

Enzyme and pathway databases

UniPathwayi UPA00733 .
SABIO-RK P51650.

Miscellaneous databases

PROi P51650.

Gene expression databases

Genevestigatori P51650.

Family and domain databases

Gene3Di 3.40.309.10. 1 hit.
3.40.605.10. 1 hit.
InterProi IPR016161. Ald_DH/histidinol_DH.
IPR016163. Ald_DH_C.
IPR016160. Ald_DH_CS_CYS.
IPR029510. Ald_DH_CS_GLU.
IPR016162. Ald_DH_N.
IPR015590. Aldehyde_DH_dom.
IPR010102. Succ_semiAld_DH.
[Graphical view ]
Pfami PF00171. Aldedh. 1 hit.
[Graphical view ]
SUPFAMi SSF53720. SSF53720. 1 hit.
TIGRFAMsi TIGR01780. SSADH. 1 hit.
PROSITEi PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit.
PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  2. "Molecular cloning of the mature NAD(+)-dependent succinic semialdehyde dehydrogenase from rat and human. cDNA isolation, evolutionary homology, and tissue expression."
    Chambliss K.L., Caudle D.L., Hinson D.D., Moomaw C.R., Slaughter C.A., Jakobs C., Gibson K.M.
    J. Biol. Chem. 270:461-467(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS LONG AND SHORT), PROTEIN SEQUENCE OF 36-84; 92-101; 129-153; 162-172; 303-311; 419-425 AND 503-526.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. Lubec G., Afjehi-Sadat L.
    Submitted (NOV-2006) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 36-48; 161-172 AND 401-418, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Spinal cord.

Entry informationi

Entry nameiSSDH_RAT
AccessioniPrimary (citable) accession number: P51650
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 8, 2008
Last modified: October 1, 2014
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3