Reviewed,
UniProtKB/Swiss-Prot P51647 (AL1A1_RAT)
Last modified
November 4, 2008.
Version 68.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Retinal dehydrogenase 1 Short name=RALDH 1 Short name=RalDH1 EC=1.2.1.36 Alternative name(s): Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase, cytosolic ALHDII ALDH-E1 | ||||
| Gene names |
| ||||
| Organism | Rattus norvegicus (Rat) | ||||
| Taxonomic identifier | 10116 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 501 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Is capable of converting 9-cis and all-trans retinal to corresponding retinoic acid with high efficiency, 9-cis retinal being 2-fold more active than all-trans retinal. |
| Catalytic activity | Retinal + NAD(+) + H(2)O = retinoate + NADH. |
| Enzyme regulation | Inhibited by chloral hydrate. |
| Pathway | |
| Subunit structure | Homotetramer By similarity. |
| Subcellular location | |
| Tissue specificity | Strongly expressed in kidney, lung, testis, intestine, stomach, and trachea, but weakly in the liver. |
| Post-translational modification | The N-terminus is blocked. |
| Sequence similarities | Belongs to the aldehyde dehydrogenase family. |
Ontologies
Keywords | |
|---|---|
| Cellular component | Cytoplasm |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| PTM | Acetylation |
| Technical term | Direct protein sequencing |
Gene Ontology (GO) | |
| Biological process | oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | retinal dehydrogenase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed | ||||||
| Chain | 2 – 501 | 500 | Retinal dehydrogenase 1 | PRO_0000056419 | |||||
Regions | |||||||||
| Nucleotide binding | 246 – 251 | 6 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 269 | 1 | Proton acceptor By similarity | ||||||
| Active site | 303 | 1 | Nucleophile By similarity | ||||||
| Site | 170 | 1 | Transition state stabilizer By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylserine Probable | ||||||
Experimental info | |||||||||
| Sequence conflict | 100 | 1 | R → C in AAA96657. Ref.1 | ||||||
| Sequence conflict | 106 | 1 | I → M AA sequence Ref.6 | ||||||
| Sequence conflict | 170 | 1 | N → E in AAA96657. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Cloning of a cDNA encoding rat aldehyde dehydrogenase with high activity for retinal oxidation." Bhat P.V., Labrecque J., Boutin J.-M., Lacroix A., Yoshida A. Gene 166:303-306(1995) [PubMed: 8543180] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Kidney. |
| [2] | "A preliminary report on the cloning of a constitutively expressed rat liver cytosolic ALDH cDNA by PCR." Kathmann E.C., Lipsky J.J. Adv. Exp. Med. Biol. 414:69-72(1997) [PubMed: 9059608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver. |
| [3] | "Cloning of a cDNA encoding a constitutively expressed rat liver cytosolic aldehyde dehydrogenase." Kathmann E.C., Lipsky J.J. Biochem. Biophys. Res. Commun. 236:527-531(1997) [PubMed: 9240474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver. |
| [4] | "Cloning of a rat cDNA encoding retinal dehydrogenase isozyme type I and its expression in E. coli." Napoli J.L., Penzes P., Wang X., Sperkova Z. Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver. |
| [5] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pituitary. |
| [6] | "A novel isoenzyme of aldehyde dehydrogenase specifically involved in the biosynthesis of 9-cis and all-trans retinoic acid." Labrecque J., Dumas F., Lacroix A., Bhat P.V. Biochem. J. 305:681-684(1995) [PubMed: 7832787] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-19; 80-91; 96-121; 205-225; 237-258 AND 394-438. Strain: Sprague-Dawley. Tissue: Kidney. |
Cross-references
Sequence databases | |
|---|---|
| L42009 mRNA. Translation: AAA96657.1. AF001896 mRNA. Translation: AAC53304.1. AF001898 mRNA. Translation: AAC53306.1. AF001897 mRNA. Translation: AAC53305.1. U79118 mRNA. Translation: AAB63423.1. BC061526 mRNA. Translation: AAH61526.1. | |
| PIR | JC4524. JC5553. |
| RefSeq | NP_071852.2. |
| UniGene | Rn.6132 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1BXS based on UniProtKB P51977. |
| ModBase | Search... |
Genome annotation databases | |
| Ensembl | ENSRNOG00000017619. Rattus norvegicus. [Contig view] |
| GeneID | 24188. |
| KEGG | rno:24188. |
Organism-specific databases | |
| RGD | 2087. Aldh1a1. |
Phylogenomic databases | |
| HOVERGEN | P51647. |
Gene expression databases | |
| ArrayExpress | P51647. |
| GermOnline | ENSRNOG00000017619. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. [Graphical view] |
| Gene3D | G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit. |
| PANTHER | PTHR11699. Aldehyde_dehyd. 1 hit. |
| Pfam | PF00171. Aldedh. 1 hit. [Graphical view] |
| PROSITE | PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 602555. |
Entry information
| Entry name | AL1A1_RAT | ||||||||
| Accession | Primary (citable) accession number: P51647 Secondary accession number(s): O09184 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with


