P51637 (CAV3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Caveolin-3

Alternative name(s):
M-caveolin

Gene names

Name:

Cav3

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. Ref.4
Subunit structure	Homooligomer By similarity. Interacts with DYSF. Interacts with DLG1 and KCNA5; forms a ternary complex. Interacts with DAG1 (via its C-terminal); the interaction prevents binding of DAG1 with DMD By similarity. Interacts with TRIM72. Interacts with MUSK; may regulate MUSK signaling. Ref.4 Ref.5
Subcellular location	Golgi apparatus membrane; Peripheral membrane protein By similarity. Cell membrane; Peripheral membrane protein By similarity. Membrane › caveola; Peripheral membrane protein By similarity. Note: Potential hairpin-like structure in the membrane. Membrane protein of caveolae By similarity.
Tissue specificity	Expressed predominantly in muscle. Ref.1
Post-translational modification	Sumoylation with SUMO3 by PIAS4 may reduce agonist-induced internalization and desensitization of adrenergic receptor ABRD2 By similarity.
Sequence similarities	Belongs to the caveolin family.

Ontologies

Keywords
Cellular component	Cell membrane Golgi apparatus Membrane
PTM	Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	actin filament organization Inferred from mutant phenotype PubMed 14517320. Source: MGI cardiac muscle cell development Inferred from mutant phenotype PubMed 19535499. Source: MGI caveola assembly Inferred from mutant phenotype PubMed 18054955. Source: MGI cell growth Inferred from electronic annotation. Source: Compara cholesterol homeostasis Inferred from mutant phenotype PubMed 15314230. Source: MGI cytoplasmic microtubule organization Inferred from mutant phenotype PubMed 14517320. Source: MGI elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara endocytosis Inferred from mutant phenotype PubMed 14600260. Source: MGI establishment of protein localization to plasma membrane Inferred from mutant phenotype Ref.4. Source: MGI glucose homeostasis Inferred from mutant phenotype PubMed 15314230. Source: MGI heart trabecula formation Inferred from mutant phenotype PubMed 19535499. Source: MGI muscle cell homeostasis Inferred from mutant phenotype PubMed 11115849PubMed 12138167PubMed 14600260. Source: MGI myoblast fusion Inferred from mutant phenotype PubMed 14517320. Source: MGI negative regulation of MAP kinase activity Inferred from electronic annotation. Source: Compara negative regulation of MAPK cascade Inferred from mutant phenotype PubMed 12138167. Source: MGI negative regulation of calcium ion transport Inferred from mutant phenotype PubMed 21084288. Source: MGI negative regulation of cardiac muscle hypertrophy Inferred from mutant phenotype PubMed 18552160. Source: MGI negative regulation of cell growth involved in cardiac muscle cell development Inferred from electronic annotation. Source: Compara negative regulation of cell size Inferred from electronic annotation. Source: Compara negative regulation of nitric-oxide synthase activity Inferred from mutant phenotype PubMed 11159934. Source: MGI negative regulation of protein kinase activity Inferred from mutant phenotype PubMed 14600260. Source: MGI negative regulation of sarcomere organization Inferred from electronic annotation. Source: Compara nucleus localization Inferred from mutant phenotype PubMed 19535499. Source: MGI plasma membrane repair Inferred from mutant phenotype Ref.4. Source: MGI positive regulation of cell proliferation Inferred from electronic annotation. Source: Compara positive regulation of microtubule polymerization Inferred from electronic annotation. Source: Compara positive regulation of myotube differentiation Inferred from mutant phenotype PubMed 14517320. Source: MGI regulation of branching involved in mammary gland duct morphogenesis Inferred from mutant phenotype PubMed 19164602. Source: MGI regulation of calcium ion import Inferred from electronic annotation. Source: Compara regulation of calcium ion transmembrane transporter activity Inferred from electronic annotation. Source: Compara regulation of cardiac muscle contraction Inferred from electronic annotation. Source: Compara regulation of heart rate Inferred from electronic annotation. Source: Compara regulation of membrane depolarization involved in regulation of cardiac muscle cell action potential Inferred from electronic annotation. Source: Compara regulation of membrane potential Inferred from genetic interaction PubMed 15277200. Source: MGI regulation of nerve growth factor receptor activity Inferred from electronic annotation. Source: Compara regulation of p38MAPK cascade Inferred from mutant phenotype PubMed 21182936. Source: MGI regulation of protein kinase B signaling cascade Inferred from mutant phenotype PubMed 21182936. Source: MGI regulation of signal transduction by receptor internalization Inferred from electronic annotation. Source: Compara regulation of skeletal muscle contraction Inferred from electronic annotation. Source: Compara regulation of sodium ion transmembrane transporter activity Inferred from electronic annotation. Source: Compara regulation of transforming growth factor beta receptor signaling pathway Inferred from mutant phenotype PubMed 21182936. Source: MGI regulation of ventricular cardiac muscle cell membrane depolarization Inferred from electronic annotation. Source: Compara regulation of ventricular cardiac muscle cell membrane repolarization Inferred from electronic annotation. Source: Compara triglyceride metabolic process Inferred from mutant phenotype PubMed 15314230. Source: MGI
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell T-tubule Inferred from direct assay PubMed 16319126PubMed 9008709. Source: MGI caveola Inferred from direct assay PubMed 21084288. Source: BHF-UCL cell surface Inferred from electronic annotation. Source: Compara dystrophin-associated glycoprotein complex Inferred from electronic annotation. Source: Compara endoplasmic reticulum Inferred from sequence orthology PubMed 22792322. Source: MGI neuromuscular junction Inferred from electronic annotation. Source: Compara vesicle Inferred from electronic annotation. Source: Compara
Molecular_function	alpha-tubulin binding Inferred from direct assay PubMed 14517320. Source: MGI calcium channel regulator activity Inferred from electronic annotation. Source: Compara sodium channel regulator activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Caveolin-3		PRO_0000144141
Regions
	Topological domain	1 – 83	83	Cytoplasmic Potential
	Intramembrane	84 – 104	21	Helical; Potential
	Topological domain	105 – 151	47	Cytoplasmic Potential
	Region	64 – 114	51	Required for interaction with DAG1 By similarity
Amino acid modifications
	Cross-link	38		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO3) By similarity
Experimental info
	Mutagenesis	27	1	R → Q: Results in aberrent localization of TRIM72 and defects in membrane repair. Ref.4
	Mutagenesis	105	1	P → L: Dominant-negative mutant that induces defects in membrane repair. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P51637 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7C1A181010CC60F1	FASTA	151	17,358
	10 20 30 40 50 60 MMTEEHTDLE ARIIKDIHCK EIDLVNRDPK NINEDIVKVD FEDVIAEPEG TYSFDGVWKV 70 80 90 100 110 120 SFTTFTVSKY WCYRLLSTLL GVPLALLWGF LFACISFCHI WAVVPCIKSY LIEIQCISHI 130 140 150 YSLCIRTFCN PLFAALGQVC SNIKVVLRRE G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"M-caveolin, a muscle-specific caveolin-related protein." Way M., Parton R.G. FEBS Lett. 376:108-112(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: BALB/c. Tissue: Skeletal muscle.
[2]	Erratum Way M., Parton R.G. FEBS Lett. 378:108-112(1996) [PubMed] [Europe PMC] [Abstract]
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Colon.
[4]	"Membrane repair defects in muscular dystrophy are linked to altered interaction between MG53, caveolin-3, and dysferlin." Cai C., Weisleder N., Ko J.-K., Komazaki S., Sunada Y., Nishi M., Takeshima H., Ma J. J. Biol. Chem. 284:15894-15902(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH TRIM72, MUTAGENESIS OF ARG-27 AND PRO-105.
[5]	"Caveolin-3 promotes nicotinic acetylcholine receptor clustering and regulates neuromuscular junction activity." Hezel M., de Groat W.C., Galbiati F. Mol. Biol. Cell 21:302-310(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH MUSK.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U36579 mRNA. Translation: AAC52352.1. BC024383 mRNA. Translation: AAH24383.1.
IPI	IPI00124974.
PIR	S68222.
RefSeq	NP_031643.1. NM_007617.2.
UniGene	Mm.3924.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P51637. 2 interactions.
MINT	MINT-2982959.
STRING	10090.ENSMUSP00000074922.
PTM databases
PhosphoSite	P51637.
Proteomic databases
PaxDb	P51637.
PRIDE	P51637.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000075477; ENSMUSP00000074922; ENSMUSG00000062694.
GeneID	12391.
KEGG	mmu:12391.
UCSC	uc009dea.1. mouse.
Organism-specific databases
CTD	859.
MGI	MGI:107570. Cav3.
Phylogenomic databases
eggNOG	NOG86001.
GeneTree	ENSGT00390000014924.
HOGENOM	HOG000036550.
HOVERGEN	HBG003422.
InParanoid	P51637.
KO	K12959.
OMA	HSLDGVW.
OrthoDB	EOG4DFPPG.
Gene expression databases
ArrayExpress	P51637.
Bgee	P51637.
CleanEx	MM_CAV3.
Genevestigator	P51637.
GermOnline	ENSMUSG00000062694. Mus musculus.
Family and domain databases
InterPro	IPR001612. Caveolin. IPR018361. Caveolin_CS. [Graphical view]
PANTHER	PTHR10844. PTHR10844. 1 hit.
Pfam	PF01146. Caveolin. 1 hit. [Graphical view]
PROSITE	PS01210. CAVEOLIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	281130.
SOURCE	Search...

Entry information

Entry name

CAV3_MOUSE

Accession

Primary (citable) accession number: P51637

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program