Reviewed, UniProtKB/Swiss-Prot P51615 (MAOX_VITVI)

Names and origin

Protein names	Recommended name:     NADP-dependent malic enzyme       Short name=NADP-ME     EC=1.1.1.40
Organism	Vitis vinifera (Grape)
Taxonomic identifier	29760 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH.
Cofactor	Divalent metal cations. Prefers magnesium or manganese By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	CytoplasmPotential.
Sequence similarities	Belongs to the malic enzymes family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	NADP-dependent malic enzyme		PRO_0000160204
Regions
	Nucleotide binding	335 – 351	17	NADP By similarity
Sites
	Active site	139	1	Proton donor By similarity
	Active site	210	1	Proton acceptor By similarity
	Metal binding	282	1	Divalent metal cation By similarity
	Metal binding	283	1	Divalent metal cation By similarity
	Metal binding	306	1	Divalent metal cation By similarity
	Binding site	192	1	NAD By similarity
	Binding site	306	1	NAD By similarity
	Binding site	447	1	NAD By similarity
	Site	306	1	Important for activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51615-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FD78B0976A7C4744	FASTA	591	65,227
	10 20 30 40 50 60 MESTLKDIRD GASVLDLDPK ATVGGGVEDL YGEDFATEDQ LVTPWTVSVA SGYSLLRDPR 70 80 90 100 110 120 HNKGLAFNDK ERDAHYLCGL LPPVVSTQEL QERKLMNSIR QYQVPLQKYM AMMDLQERNE 130 140 150 160 170 180 RLFYKLLIDN VEELLPVVYT PTVGEACQKY GSIFRRPQGL YISLKEKGKI LEVLKNWPER 190 200 210 220 230 240 RIQVIVVTDG ERILGLGDLG CQGMGIPVGK LSLYTALGGV RPSACLPITI DVGTNNEKLL 250 260 270 280 290 300 ANEFYIGLKQ RRATGKEYSE FLQEFMSPVK QNYGEKVLIQ FEDFANHNAF DLLAKYGTTH 310 320 330 340 350 360 LAFNDDIQGT ASVVLAGIVS ALRLLGGTLA DHKFLFLGAG EAGTGIAELI ALEMSKQTKC 370 380 390 400 410 420 PIEETRKKIW LVDSKGLIVG SRKDSLQQFK KPWAHEHEPV KDLLDAVKVI KPTVLIGSSG 430 440 450 460 470 480 VGKAFTKEVI EAMASCNEKP LILALSNPTS QSECTAEEAY TWTQGRAIFA SGSPFDPVEY 490 500 510 520 530 540 NGKTFVPGQA NNAYIFPGLG MGLVISGAIR VHDEMLLAAS EALARQVTQE NFDKGLIYPP 550 560 570 580 590 FSNIRKISAH IAANVAAKAY ELGLATRLPQ PENLVKYAES CMYSPVYRSY R

References

[1]	"Cloning of a full-length cDNA for malic enzyme (EC 1.1.1.40) from grape berries." Franke K.E., Adams D.O. Plant Physiol. 107:1009-1010(1995) [PubMed: 7716227] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Thompson. Tissue: Fruit.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L34836 mRNA. Translation: AAA67087.1.
3D structure databases
HSSP	HSSP built from PDB template 1GQ2 based on UniProtKB P40927.
ModBase	Search...
Family and domain databases
InterPro	IPR015884. Malic_enzyme_CS. IPR012301. Malic_N. IPR012302. Malic_NAD_bd. IPR001891. Malic_OxRdtase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00390. malic. 1 hit. PF03949. Malic_M. 1 hit. [Graphical view]
PRINTS	PR00072. MALOXRDTASE.
PROSITE	PS00331. MALIC_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MAOX_VITVI

Accession

Primary (citable) accession number: P51615

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)