Reviewed, UniProtKB/Swiss-Prot P51613 (CHIB_VITVI)

Names and origin

Protein names

Recommended name:
    Basic endochitinase
    EC=3.2.1.14

Gene names

Name:

CHIT1B

Organism

Vitis vinifera (Grape)

Taxonomic identifier

29760 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Vitales › Vitaceae › Vitis

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Defense against chitin containing fungal pathogens.
Catalytic activity	Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 19 family. Chitinase class I subfamily. Contains 1 chitin-binding type-1 domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Chitin degradation Plant defense Polysaccharide degradation
Domain	Signal
Ligand	Chitin-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro chitin catabolic process Inferred from electronic annotation. Source: UniProtKB-KW defense response Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin binding Inferred from electronic annotation. Source: UniProtKB-KW chitinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 314	294	Basic endochitinase		PRO_0000005338
Regions
	Domain	21 – 61	41	Chitin-binding type-1
Amino acid modifications
	Disulfide bond	23 ↔ 38		By similarity
	Disulfide bond	32 ↔ 44		By similarity
	Disulfide bond	37 ↔ 51		By similarity
	Disulfide bond	55 ↔ 59		By similarity
	Disulfide bond	86 ↔ 148		By similarity
	Disulfide bond	160 ↔ 168		By similarity
	Disulfide bond	267 ↔ 299		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51613-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3802484ED69BC6C0	FASTA	314	33,429
	10 20 30 40 50 60 MGLWALVAFC LLSLILVGSA EQCGGQAGGR VCPGGACCSK FGWCGNTADY CGSGCQSQCS 70 80 90 100 110 120 STGDIGQLIT RSMFNDMLKH RNEGSCPGKG FYTYDAFIAA AKAFPGFGTT GDTTTRKREI 130 140 150 160 170 180 AAFLAQTSHE TTGGWASAPD GPYAWGYCYL REQGSPGAYC VPSAQWPCAA GRKYYGRGPI 190 200 210 220 230 240 QISYNYNYGQ AGKAIGVDLV NNPDLVATDA VISFKTAFWF WMTPQSPKPS CHNVITGGWT 250 260 270 280 290 300 PSGADRSAGR LPGFGVITNI INGGVECGKG VVPQVQDRIG FYKRYCDILR VSYGNNLDCN 310 NQRPFGSGLL LDTI

References

[1]	"Differential expression of chitinases in Vitis vinifera L. responding to systemic acquired resistance activators or fungal challenge." Busam G., Kassemeyer H.H., Matern U. Plant Physiol. 115:1029-1038(1997) [PubMed: 9390436] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Pinot.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z54234 mRNA. Translation: CAA90970.1.
RefSeq	XP_002281770.1.
3D structure databases
HSSP	HSSP built from PDB template 1CNS based on UniProtKB P23951.
ModBase	Search...
Protein family/group databases
CAZy	CBM18. Carbohydrate-Binding Module Family 18. GH19. Glycoside Hydrolase Family 19.
Genome annotation databases
GeneID	100232985.
KEGG	vvi:100232985.
Phylogenomic databases
OMA	KRYCDIL.
Enzyme and pathway databases
BRENDA	3.2.1.14. 81671.
Family and domain databases
InterPro	IPR018371. Chitin-binding_1_CS. IPR001002. Chitin_bd_1. IPR016283. Glyco_hydro_19. IPR000726. Glyco_hydro_19_cat. [Graphical view]
Gene3D	G3DSA:3.30.60.10. Chitin_bd_1. 1 hit.
PANTHER	PTHR22595. Glyco_hydro_19_cat. 1 hit.
Pfam	PF00187. Chitin_bind_1. 1 hit. PF00182. Glyco_hydro_19. 1 hit. [Graphical view]
PIRSF	PIRSF001060. Endochitinase. 1 hit.
PRINTS	PR00451. CHITINBINDNG.
ProDom	PD000609. Chitin_binding_1. 1 hit. PD354900. Glyco_hydro_19. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00270. ChtBD1. 1 hit. [Graphical view]
PROSITE	PS00026. CHIT_BIND_I_1. 1 hit. PS50941. CHIT_BIND_I_2. 1 hit. PS00773. CHITINASE_19_1. 1 hit. PS00774. CHITINASE_19_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHIB_VITVI

Accession

Primary (citable) accession number: P51613

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)