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Protein

GTP cyclohydrolase 1

Gene

FOL2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTP cyclohydrolase 1 is the first enzyme in the biosynthetic pathway leading to folic acid.

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

Enzyme regulationi

GTP shows a positive allosteric effect, and tetrahydrobiopterin inhibits the enzyme activity.By similarity

Pathwayi: 7,8-dihydroneopterin triphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP.
Proteins known to be involved in this subpathway in this organism are:
  1. GTP cyclohydrolase 1 (FOL2)
This subpathway is part of the pathway 7,8-dihydroneopterin triphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 7,8-dihydroneopterin triphosphate from GTP, the pathway 7,8-dihydroneopterin triphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi132ZincBy similarity1
Metal bindingi135ZincBy similarity1
Metal bindingi203ZincBy similarity1

GO - Molecular functioni

  • GTP binding Source: GO_Central
  • GTP cyclohydrolase I activity Source: SGD
  • zinc ion binding Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processFolate biosynthesis
LigandGTP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:YGR267C-MONOMER.
ReactomeiR-SCE-1474151. Tetrahydrobiopterin (BH4) synthesis, recycling, salvage and regulation.
UniPathwayiUPA00848; UER00151.

Names & Taxonomyi

Protein namesi
Recommended name:
GTP cyclohydrolase 1 (EC:3.5.4.16)
Alternative name(s):
GTP cyclohydrolase I
Short name:
GTP-CH-I
Gene namesi
Name:FOL2
Ordered Locus Names:YGR267C
ORF Names:G9349
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR267C.
SGDiS000003499. FOL2.

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001194891 – 243GTP cyclohydrolase 1Add BLAST243

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei15PhosphothreonineCombined sources1
Modified residuei23PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP51601.
PRIDEiP51601.

PTM databases

iPTMnetiP51601.

Interactioni

Subunit structurei

Toroid-shaped homodecamer, composed of two pentamers of five dimers.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
PIL1P532522EBI-7429,EBI-23225

Protein-protein interaction databases

BioGridi33517. 45 interactors.
DIPiDIP-1318N.
IntActiP51601. 27 interactors.
MINTiMINT-389985.
STRINGi4932.YGR267C.

Structurei

3D structure databases

ProteinModelPortaliP51601.
SMRiP51601.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000013481.
HOGENOMiHOG000221222.
InParanoidiP51601.
KOiK01495.
OMAiYEDNIRD.
OrthoDBiEOG092C4H51.

Family and domain databases

HAMAPiMF_00223. FolE. 1 hit.
InterProiView protein in InterPro
IPR001474. GTP_CycHdrlase_I.
IPR018234. GTP_CycHdrlase_I_CS.
IPR020602. GTP_CycHdrlase_I_dom.
PANTHERiPTHR11109. PTHR11109. 1 hit.
PfamiView protein in Pfam
PF01227. GTP_cyclohydroI. 1 hit.
TIGRFAMsiTIGR00063. folE. 1 hit.
PROSITEiView protein in PROSITE
PS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P51601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHNIQLVQEI ERHETPLNIR PTSPYTLNPP VERDGFSWPS VGTRQRAEET
60 70 80 90 100
EEEEKERIQR ISGAIKTILT ELGEDVNREG LLDTPQRYAK AMLYFTKGYQ
110 120 130 140 150
TNIMDDVIKN AVFEEDHDEM VIVRDIEIYS LCEHHLVPFF GKVHIGYIPN
160 170 180 190 200
KKVIGLSKLA RLAEMYARRL QVQERLTKQI AMALSDILKP LGVAVVMEAS
210 220 230 240
HMCMVSRGIQ KTGSSTVTSC MLGGFRAHKT REEFLTLLGR RSI
Length:243
Mass (Da):27,769
Last modified:October 1, 1996 - v1
Checksum:iA71AB8750AECD3F6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti159 – 160LA → QG in CAA89826 (PubMed:7542887).Curated2
Sequence conflicti206S → M in CAA89826 (PubMed:7542887).Curated1
Sequence conflicti217V → A in CAA87397 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z47201 Genomic DNA. Translation: CAA87397.1.
X94314 Genomic DNA. Translation: CAA63975.1.
Y07893 Genomic DNA. Translation: CAA69198.1.
Z73052 Genomic DNA. Translation: CAA97297.1.
AY692993 Genomic DNA. Translation: AAT93012.1.
Z49756 mRNA. Translation: CAA89826.1.
BK006941 Genomic DNA. Translation: DAA08356.1.
PIRiJC4585.
RefSeqiNP_011783.1. NM_001181396.1.

Genome annotation databases

EnsemblFungiiYGR267C; YGR267C; YGR267C.
GeneIDi853183.
KEGGisce:YGR267C.

Similar proteinsi

Entry informationi

Entry nameiGCH1_YEAST
AccessioniPrimary (citable) accession number: P51601
Secondary accession number(s): D6VV45
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 27, 2017
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names