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Reviewed, UniProtKB/Swiss-Prot P51601 (GCH1_YEAST)

Last modified November 3, 2009. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    GTP cyclohydrolase 1
    EC=3.5.4.16
Alternative name(s):
    GTP cyclohydrolase I
      Short name=GTP-CH-I
Gene names
Name: FOL2
Ordered Locus Names: YGR267C
ORF Names: G9349
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length243 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Catalytic activity

GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate.

Enzyme regulation

GTP shows a positive allosteric effect, and tetrahydrobiopterin inhibits the enzyme activity By similarity.

Pathway

Cofactor biosynthesis; 7,8-dihydroneopterin triphosphate biosynthesis; 7,8-dihydroneopterin triphosphate from GTP: step 1/1.

Subunit structure

Toroid-shaped homodecamer, composed of two pentamers of five dimers By similarity.

Miscellaneous

Present with 2840 molecules/cell in log phase SD medium. Ref.7

Sequence similarities

Belongs to the GTP cyclohydrolase I family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SRP1Q028211EBI-7429,EBI-1797

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 243243GTP cyclohydrolase 1
PRO_0000119489

Sites

Metal binding1321Zinc By similarity
Metal binding1351Zinc By similarity
Metal binding2031Zinc By similarity

Amino acid modifications

Modified residue151Phosphothreonine Ref.8 Ref.9 Ref.10
Modified residue221Phosphothreonine Ref.9 Ref.10
Modified residue231Phosphoserine Ref.8 Ref.9 Ref.10
Modified residue251Phosphotyrosine Ref.10

Experimental info

Sequence conflict159 – 1602LA → QG in CAA89826. Ref.6
Sequence conflict2061S → M in CAA89826. Ref.6
Sequence conflict2171V → A in CAA87397. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P51601-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: A71AB8750AECD3F6

FASTA24327,769
        10         20         30         40         50         60 
MHNIQLVQEI ERHETPLNIR PTSPYTLNPP VERDGFSWPS VGTRQRAEET EEEEKERIQR 

        70         80         90        100        110        120 
ISGAIKTILT ELGEDVNREG LLDTPQRYAK AMLYFTKGYQ TNIMDDVIKN AVFEEDHDEM 

       130        140        150        160        170        180 
VIVRDIEIYS LCEHHLVPFF GKVHIGYIPN KKVIGLSKLA RLAEMYARRL QVQERLTKQI 

       190        200        210        220        230        240 
AMALSDILKP LGVAVVMEAS HMCMVSRGIQ KTGSSTVTSC MLGGFRAHKT REEFLTLLGR 


RSI 

« Hide

References

« Hide 'large scale' references
[1]Witter K., Guetlich M., Stucka R., Ziegler I., Bacher A.
Submitted (MAY-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: C836.
[2]"Disruption of the GTP-cyclohydrolase I gene in Saccharomyces cerevisiae."
Nardese V., Gutlich M., Brambilla A., Agostoni Carbone M.L.
Biochem. Biophys. Res. Commun. 218:273-279(1996) [PubMed: 8573145] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"A 9359 bp fragment from the right arm of Saccharomyces cerevisiae chromosome VII includes the FOL2 and YTA7 genes and three unknown open reading frames."
Agostoni Carbone M.L., Lucchini G., Melchioretto P., Nardese V., Vanoni M., Panzeri L.
Yeast 14:587-591(1998) [PubMed: 9605509] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed: 9169869] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Homology cloning of GTP-cyclohydrolase I from various unrelated eukaryotes by reverse-transcription polymerase chain reaction using a general set of degenerate primers."
Maier J., Witter K., Guetlich M., Ziegler I., Werner T., Ninnemann H.
Biochem. Biophys. Res. Commun. 212:705-711(1995) [PubMed: 7542887] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 115-208.
Strain: ATCC 204508 / S288c.
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-15 AND SER-23, MASS SPECTROMETRY.
[9]"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases."
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-15; THR-22 AND SER-23, MASS SPECTROMETRY.
[10]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-15; THR-22; SER-23 AND TYR-25, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

Z47201 Genomic DNA. Translation: CAA87397.1.
X94314 Genomic DNA. Translation: CAA63975.1.
Y07893 Genomic DNA. Translation: CAA69198.1.
Z73052 Genomic DNA. Translation: CAA97297.1.
AY692993 Genomic DNA. Translation: AAT93012.1.
Z49756 mRNA. Translation: CAA89826.1.
PIRJC4585.
RefSeqNP_011783.1.

3D structure databases

HSSPHSSP built from PDB template 1IS8 based on UniProtKB P22288.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:1318N.
IntActP51601. 18 interactions.
STRINGP51601.

Proteomic databases

PeptideAtlasP51601.
PRIDEP51601.

Genome annotation databases

EnsemblYGR267C; YGR267C; YGR267C; Saccharomyces cerevisiae. [Genome view]
GeneID853183.
GenomeReviewsGene locus YGR267C in contig Y13135_GR.
KEGGsce:YGR267C.
NMPDRfig|4932.3.peg.2912.

Organism-specific databases

CYGDYGR267c.
SGDS000003499. FOL2.

Phylogenomic databases

HOGENOMP51601.
OMARITSTIA.

Enzyme and pathway databases

BRENDA3.5.4.16. 250.

Gene expression databases

ArrayExpressP51601.
GenevestigatorP51601.
GermOnlineYGR267C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001474. GTP_CycHdrlase_I.
IPR020602. GTP_CycHdrlase_I/CN_OxRdtase.
IPR018234. GTP_CycHdrlase_I_CS.
[Graphical view]
PANTHERPTHR11109. GTP_cyclohydro_I. 1 hit.
PfamPF01227. GTP_cyclohydroI. 1 hit.
[Graphical view]
ProDomPD003330. GTP_cyclohydroI. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00063. folE. 1 hit.
PROSITEPS00859. GTP_CYCLOHYDROL_1_1. 1 hit.
PS00860. GTP_CYCLOHYDROL_1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio973323.

Entry information

Entry nameGCH1_YEAST
AccessionPrimary (citable) accession number: P51601
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 3, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents