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Protein

Cytochrome P450 2J3

Gene

Cyp2j3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation mainly to 14,15-, 11,12-, and 8,9-epoxyeicosatrienoic acids (EET). It also acts as an omega-1-hydroxylase by metabolizing arachidonic acid to 19-hydroxyeicosatetraenoic acid (19-OH-AA).

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi448 – 4481Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. arachidonic acid epoxygenase activity Source: GO_Central
  2. aromatase activity Source: UniProtKB-EC
  3. heme binding Source: GO_Central
  4. iron ion binding Source: InterPro
  5. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  6. oxygen binding Source: GO_Central
  7. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. arachidonic acid metabolic process Source: GO_Central
  2. exogenous drug catabolic process Source: GO_Central
  3. oxidation-reduction process Source: GO_Central
  4. xenobiotic metabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2J3 (EC:1.14.14.1)
Alternative name(s):
CYPIIJ3
Gene namesi
Name:Cyp2j3
Synonyms:Cyp2j9
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi631442. Cyp2j3.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: UniProtKB-SubCell
  3. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Cytochrome P450 2J3PRO_0000051770Add
BLAST

Proteomic databases

PaxDbiP51590.
PRIDEiP51590.

Expressioni

Tissue specificityi

Abundantly expressed in heart and liver.

Gene expression databases

GenevestigatoriP51590.

Interactioni

Protein-protein interaction databases

MINTiMINT-4577944.
STRINGi10116.ENSRNOP00000013087.

Structurei

3D structure databases

ProteinModelPortaliP51590.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiP51590.
KOiK07418.
PhylomeDBiP51590.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008071. Cyt_P450_E_grp-I_CYP2J-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01688. EP450ICYP2J.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51590-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLVTAGSLLG AIWTVLHLRI LLLAAVTFLF LADFLKHRRP KNYPPGPWRL
60 70 80 90 100
PLVGCLFHLD PKQPHLSLQQ FVKKYGNVLS LDFANIPSVV VTGMPLIKEI
110 120 130 140 150
FTQMEHNFLN RPVTLLRKHL FNKNGLIFSS GQTWKEQRRF ALMTLRNFGL
160 170 180 190 200
GKKSLEQRIQ EEAYHLVEAI KDEGGLPFDP HFNINKAVSN IICSVTFGER
210 220 230 240 250
FEYHDSQFQE MLRLLDEAMC LESSMMCQLY NIFPRILQYL PGSHQTLFSN
260 270 280 290 300
WRKLKLFISD IIKNHRRDWD PDEPRDFIDA FLKEMAKYPD KTTTSFNEEN
310 320 330 340 350
LICSTLDLFF AGTETTSTTL RWALLCMALY PEVQEKMQAE IDRVIGQGRQ
360 370 380 390 400
PNLADRDSMP YTNAVIHEVQ RIGNIIPFNV PREVAVDTYL AGFNLPKGTM
410 420 430 440 450
ILTNLTALHR DPKEWATPDT FNPEHFLENG QFKKRESFLP FSMGKRACLG
460 470 480 490 500
EQLARSELFI FITSLIQKFT FKPPVNEKLS LQFRMSVTIS PVSHRLCAIP

RL
Length:502
Mass (Da):57,969
Last modified:October 1, 1996 - v1
Checksum:iCCC0AE67977FFF31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39943 mRNA. Translation: AAB48545.1.
BC089766 mRNA. Translation: AAH89766.1.
RefSeqiNP_786942.1. NM_175766.3.
UniGeneiRn.28757.

Genome annotation databases

GeneIDi313375.
KEGGirno:313375.
UCSCiRGD:631442. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U39943 mRNA. Translation: AAB48545.1.
BC089766 mRNA. Translation: AAH89766.1.
RefSeqiNP_786942.1. NM_175766.3.
UniGeneiRn.28757.

3D structure databases

ProteinModelPortaliP51590.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4577944.
STRINGi10116.ENSRNOP00000013087.

Proteomic databases

PaxDbiP51590.
PRIDEiP51590.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi313375.
KEGGirno:313375.
UCSCiRGD:631442. rat.

Organism-specific databases

CTDi313375.
RGDi631442. Cyp2j3.

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiP51590.
KOiK07418.
PhylomeDBiP51590.

Miscellaneous databases

NextBioi666056.

Gene expression databases

GenevestigatoriP51590.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008071. Cyt_P450_E_grp-I_CYP2J-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01688. EP450ICYP2J.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, expression, and functional significance of a cytochrome P450 highly expressed in rat heart myocytes."
    Wu S., Chen W., Murphy E., Gabel S., Tomer K.B., Foley J., Steenbergen C., Falck J.R., Moomaw C.R., Zeldin D.C.
    J. Biol. Chem. 272:12551-12559(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer 344.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.

Entry informationi

Entry nameiCP2J3_RAT
AccessioniPrimary (citable) accession number: P51590
Secondary accession number(s): Q5EBD7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 4, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.