P51590 (CP2J3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2J3
EC=1.14.14.1

Alternative name(s):
CYPIIJ3

Gene names

Name:	Cyp2j3
Synonyms:	Cyp2j9

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation mainly to 14,15-, 11,12-, and 8,9-epoxyeicosatrienoic acids (EET). It also acts as an omega-1-hydroxylase by metabolizing arachidonic acid to 19-hydroxyeicosatetraenoic acid (19-OH-AA).
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Abundantly expressed in heart and liver.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Cytochrome P450 2J3		PRO_0000051770
Sites
	Metal binding	448	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51590 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: CCC0AE67977FFF31	FASTA	502	57,969
	10 20 30 40 50 60 MLVTAGSLLG AIWTVLHLRI LLLAAVTFLF LADFLKHRRP KNYPPGPWRL PLVGCLFHLD 70 80 90 100 110 120 PKQPHLSLQQ FVKKYGNVLS LDFANIPSVV VTGMPLIKEI FTQMEHNFLN RPVTLLRKHL 130 140 150 160 170 180 FNKNGLIFSS GQTWKEQRRF ALMTLRNFGL GKKSLEQRIQ EEAYHLVEAI KDEGGLPFDP 190 200 210 220 230 240 HFNINKAVSN IICSVTFGER FEYHDSQFQE MLRLLDEAMC LESSMMCQLY NIFPRILQYL 250 260 270 280 290 300 PGSHQTLFSN WRKLKLFISD IIKNHRRDWD PDEPRDFIDA FLKEMAKYPD KTTTSFNEEN 310 320 330 340 350 360 LICSTLDLFF AGTETTSTTL RWALLCMALY PEVQEKMQAE IDRVIGQGRQ PNLADRDSMP 370 380 390 400 410 420 YTNAVIHEVQ RIGNIIPFNV PREVAVDTYL AGFNLPKGTM ILTNLTALHR DPKEWATPDT 430 440 450 460 470 480 FNPEHFLENG QFKKRESFLP FSMGKRACLG EQLARSELFI FITSLIQKFT FKPPVNEKLS 490 500 LQFRMSVTIS PVSHRLCAIP RL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, expression, and functional significance of a cytochrome P450 highly expressed in rat heart myocytes." Wu S., Chen W., Murphy E., Gabel S., Tomer K.B., Foley J., Steenbergen C., Falck J.R., Moomaw C.R., Zeldin D.C. J. Biol. Chem. 272:12551-12559(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U39943 mRNA. Translation: AAB48545.1. BC089766 mRNA. Translation: AAH89766.1.
IPI	IPI00203420.
RefSeq	NP_786942.1. NM_175766.3.
UniGene	Rn.28757.
3D structure databases
ProteinModelPortal	P51590.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-4577944.
STRING	10116.ENSRNOP00000013087.
Proteomic databases
PaxDb	P51590.
PRIDE	P51590.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	313375.
KEGG	rno:313375.
UCSC	RGD:631442. rat.
Organism-specific databases
CTD	313375.
RGD	631442. Cyp2j3.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036991.
HOVERGEN	HBG015789.
InParanoid	P51590.
KO	K07418.
OrthoDB	EOG49ZXP6.
Gene expression databases
ArrayExpress	P51590.
Genevestigator	P51590.
GermOnline	ENSRNOG00000031004. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008071. Cyt_P450_E_grp-I_CYP2J-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01688. EP450ICYP2J. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	666056.

Entry information

Entry name

CP2J3_RAT

Accession

Primary (citable) accession number: P51590
Secondary accession number(s): Q5EBD7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program