Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P51589 (CP2J2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2J2

EC=1.14.14.1
Alternative name(s):
Arachidonic acid epoxygenase
CYPIIJ2
Gene names
Name:CYP2J2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Tissue specificity

Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.

Sequence similarities

Belongs to the cytochrome P450 family.

Ontologies

Keywords
   Cellular componentEndoplasmic reticulum
Membrane
Microsome
   Coding sequence diversityPolymorphism
   LigandHeme
Iron
Metal-binding
NADP
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processarachidonic acid metabolic process

Traceable author statement. Source: Reactome

epoxygenase P450 pathway

Inferred from direct assay Ref.4. Source: BHF-UCL

icosanoid metabolic process

Traceable author statement Ref.1. Source: ProtInc

linoleic acid metabolic process

Inferred from direct assay Ref.4. Source: BHF-UCL

regulation of heart contraction

Traceable author statement Ref.1. Source: ProtInc

small molecule metabolic process

Traceable author statement. Source: Reactome

xenobiotic metabolic process

Traceable author statement Ref.4. Source: BHF-UCL

   Cellular_componentendoplasmic reticulum membrane

Traceable author statement. Source: Reactome

   Molecular_functionarachidonic acid 11,12-epoxygenase activity

Inferred from direct assay Ref.4. Source: BHF-UCL

arachidonic acid 14,15-epoxygenase activity

Inferred from direct assay Ref.4. Source: BHF-UCL

arachidonic acid epoxygenase activity

Inferred from direct assay Ref.4. Source: BHF-UCL

aromatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

heme binding

Inferred from electronic annotation. Source: InterPro

iron ion binding

Inferred from electronic annotation. Source: InterPro

linoleic acid epoxygenase activity

Inferred from direct assay Ref.4. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502Cytochrome P450 2J2
PRO_0000051769

Sites

Metal binding4481Iron (heme axial ligand) By similarity

Natural variations

Natural variant491R → S. Ref.5
Corresponds to variant rs11572190 [ dbSNP | Ensembl ].
VAR_029159
Natural variant1131V → M.
Corresponds to variant rs11572242 [ dbSNP | Ensembl ].
VAR_029160
Natural variant1241N → S.
Corresponds to variant rs2228113 [ dbSNP | Ensembl ].
VAR_022084
Natural variant1431T → A in allele CYP2J2*2; significantly reduced metabolism of both arachidonic acid and linoleic acid. Ref.4
Corresponds to variant rs55753213 [ dbSNP | Ensembl ].
VAR_014317
Natural variant1581R → C in allele CYP2J2*3; significantly reduced metabolism of both arachidonic acid and linoleic acid. Ref.4
Corresponds to variant rs56307989 [ dbSNP | Ensembl ].
VAR_014318
Natural variant1921I → N in allele CYP2J2*4; significantly reduced metabolism of arachidonic acid only. Ref.4
Corresponds to variant rs66515830 [ dbSNP | Ensembl ].
VAR_014319
Natural variant3421D → N in allele CYP2J2*5; no change in activity. Ref.4
Corresponds to variant rs56053398 [ dbSNP | Ensembl ].
VAR_014320
Natural variant4041N → Y in allele CYP2J2*6; significantly reduced metabolism of both arachidonic acid and linoleic acid. Ref.4
VAR_014321

Experimental info

Sequence conflict2641K → E in BAG37780. Ref.6
Sequence conflict3181T → A in BAG37780. Ref.6
Sequence conflict3391A → V in BAB85489. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P51589 [UniParc].

Last modified May 27, 2002. Version 2.
Checksum: B5F006581E249EC0

FASTA50257,611
        10         20         30         40         50         60 
MLAAMGSLAA ALWAVVHPRT LLLGTVAFLL AADFLKRRRP KNYPPGPWRL PFLGNFFLVD 

        70         80         90        100        110        120 
FEQSHLEVQL FVKKYGNLFS LELGDISAVL ITGLPLIKEA LIHMDQNFGN RPVTPMREHI 

       130        140        150        160        170        180 
FKKNGLIMSS GQAWKEQRRF TLTALRNFGL GKKSLEERIQ EEAQHLTEAI KEENGQPFDP 

       190        200        210        220        230        240 
HFKINNAVSN IICSITFGER FEYQDSWFQQ LLKLLDEVTY LEASKTCQLY NVFPWIMKFL 

       250        260        270        280        290        300 
PGPHQTLFSN WKKLKLFVSH MIDKHRKDWN PAETRDFIDA YLKEMSKHTG NPTSSFHEEN 

       310        320        330        340        350        360 
LICSTLDLFF AGTETTSTTL RWALLYMALY PEIQEKVQAE IDRVIGQGQQ PSTAARESMP 

       370        380        390        400        410        420 
YTNAVIHEVQ RMGNIIPLNV PREVTVDTTL AGYHLPKGTM ILTNLTALHR DPTEWATPDT 

       430        440        450        460        470        480 
FNPDHFLENG QFKKREAFMP FSIGKRACLG EQLARTELFI FFTSLMQKFT FRPPNNEKLS 

       490        500 
LKFRMGITIS PVSHRLCAVP QV 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart."
Wu S., Moomaw C.R., Tomer K.B., Falck J.R., Zeldin D.C.
J. Biol. Chem. 271:3460-3468(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-20.
Tissue: Liver.
[2]Wu S., Zeldin D.C., Moomaw C.R., Tomer K.B., Falck J.R.
Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO 339 AND 378.
[3]"Involvement of CYP2J2 on the intestinal first-pass metabolism of antihistamine drug, astemizole."
Matsumoto S., Hirama T., Matsubara T., Nagata K., Yamazoe Y.
Drug Metab. Dispos. 30:1240-1245(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Cloning of CYP2J2 gene and identification of functional polymorphisms."
King L.M., Ma J., Srettabunjong S., Graves J., Bradbury J.A., Li L., Spiecker M., Liao J.K., Mohrenweiser H., Zeldin D.C.
Mol. Pharmacol. 61:840-852(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-143; CYS-158; ASN-192; ASN-342 AND TYR-404, CHARACTERIZATION OF VARIANTS.
[5]NIEHS SNPs program
Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-49.
[6]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Corpus callosum.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Skin.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U37143 mRNA. Translation: AAC50370.2.
AB080265 mRNA. Translation: BAB85489.1.
AF272142 Genomic DNA. Translation: AAM44456.1.
AY426985 Genomic DNA. Translation: AAQ93356.1.
AK315387 mRNA. Translation: BAG37780.1.
BC032594 mRNA. Translation: AAH32594.1.
CCDSCCDS613.1.
RefSeqNP_000766.2. NM_000775.2.
UniGeneHs.152096.

3D structure databases

ProteinModelPortalP51589.
SMRP51589. Positions 62-498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107946. 2 interactions.
IntActP51589. 2 interactions.
STRING9606.ENSP00000360247.

Chemistry

BindingDBP51589.
ChEMBLCHEMBL3491.

PTM databases

PhosphoSiteP51589.

Polymorphism databases

DMDM21264413.

Proteomic databases

PaxDbP51589.
PRIDEP51589.

Protocols and materials databases

DNASU1573.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000371204; ENSP00000360247; ENSG00000134716.
GeneID1573.
KEGGhsa:1573.
UCSCuc001czq.3. human.

Organism-specific databases

CTD1573.
GeneCardsGC01M060284.
HGNCHGNC:2634. CYP2J2.
MIM601258. gene.
neXtProtNX_P51589.
PharmGKBPA27112.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036991.
HOVERGENHBG015789.
InParanoidP51589.
KOK07418.
OMAMSKHTGN.
OrthoDBEOG7RBZ85.
PhylomeDBP51589.
TreeFamTF352043.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.

Gene expression databases

ArrayExpressP51589.
BgeeP51589.
CleanExHS_CYP2J2.
GenevestigatorP51589.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008071. Cyt_P450_E_grp-I_CYP2J-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01688. EP450ICYP2J.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCYP2J2.
GenomeRNAi1573.
NextBio35468234.
PROP51589.
SOURCESearch...

Entry information

Entry nameCP2J2_HUMAN
AccessionPrimary (citable) accession number: P51589
Secondary accession number(s): B2RD33, Q8TF13
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 27, 2002
Last modified: July 9, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 1

Human chromosome 1: entries, gene names and cross-references to MIM