P51589 (CP2J2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2J2
EC=1.14.14.1

Alternative name(s):
Arachidonic acid epoxygenase
CYPIIJ2

Gene names

Name:

CYP2J2

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Coding sequence diversity	Polymorphism
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	arachidonic acid metabolic process Traceable author statement. Source: Reactome epoxygenase P450 pathway Inferred from direct assay Ref.4. Source: BHF-UCL icosanoid metabolic process Traceable author statement Ref.1. Source: ProtInc linoleic acid metabolic process Inferred from direct assay Ref.4. Source: BHF-UCL regulation of heart contraction Traceable author statement Ref.1. Source: ProtInc small molecule metabolic process Traceable author statement. Source: Reactome xenobiotic metabolic process Traceable author statement Ref.4. Source: BHF-UCL
Cellular_component	endoplasmic reticulum membrane Traceable author statement. Source: Reactome
Molecular_function	arachidonic acid 11,12-epoxygenase activity Inferred from direct assay Ref.4. Source: BHF-UCL arachidonic acid 14,15-epoxygenase activity Inferred from direct assay Ref.4. Source: BHF-UCL arachidonic acid epoxygenase activity Inferred from direct assay Ref.4. Source: BHF-UCL aromatase activity Inferred from electronic annotation. Source: UniProtKB-EC heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro linoleic acid epoxygenase activity Inferred from direct assay Ref.4. Source: BHF-UCL oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Cytochrome P450 2J2		PRO_0000051769
Sites
	Metal binding	448	1	Iron (heme axial ligand) By similarity
Natural variations
	Natural variant	49	1	R → S. Ref.5 Corresponds to variant rs11572190 [ dbSNP | Ensembl ].		VAR_029159
	Natural variant	113	1	V → M. Corresponds to variant rs11572242 [ dbSNP | Ensembl ].		VAR_029160
	Natural variant	124	1	N → S. Corresponds to variant rs2228113 [ dbSNP | Ensembl ].		VAR_022084
	Natural variant	143	1	T → A in allele CYP2J2*2; significantly reduced metabolism of both arachidonic acid and linoleic acid. Ref.4 Corresponds to variant rs55753213 [ dbSNP | Ensembl ].		VAR_014317
	Natural variant	158	1	R → C in allele CYP2J2*3; significantly reduced metabolism of both arachidonic acid and linoleic acid. Ref.4 Corresponds to variant rs56307989 [ dbSNP | Ensembl ].		VAR_014318
	Natural variant	192	1	I → N in allele CYP2J2*4; significantly reduced metabolism of arachidonic acid only. Ref.4 Corresponds to variant rs66515830 [ dbSNP | Ensembl ].		VAR_014319
	Natural variant	342	1	D → N in allele CYP2J2*5; no change in activity. Ref.4 Corresponds to variant rs56053398 [ dbSNP | Ensembl ].		VAR_014320
	Natural variant	404	1	N → Y in allele CYP2J2*6; significantly reduced metabolism of both arachidonic acid and linoleic acid. Ref.4		VAR_014321
Experimental info
	Sequence conflict	264	1	K → E in BAG37780. Ref.6
	Sequence conflict	318	1	T → A in BAG37780. Ref.6
	Sequence conflict	339	1	A → V in BAB85489. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P51589 [UniParc]. Last modified May 27, 2002. Version 2. Checksum: B5F006581E249EC0	FASTA	502	57,611
	10 20 30 40 50 60 MLAAMGSLAA ALWAVVHPRT LLLGTVAFLL AADFLKRRRP KNYPPGPWRL PFLGNFFLVD 70 80 90 100 110 120 FEQSHLEVQL FVKKYGNLFS LELGDISAVL ITGLPLIKEA LIHMDQNFGN RPVTPMREHI 130 140 150 160 170 180 FKKNGLIMSS GQAWKEQRRF TLTALRNFGL GKKSLEERIQ EEAQHLTEAI KEENGQPFDP 190 200 210 220 230 240 HFKINNAVSN IICSITFGER FEYQDSWFQQ LLKLLDEVTY LEASKTCQLY NVFPWIMKFL 250 260 270 280 290 300 PGPHQTLFSN WKKLKLFVSH MIDKHRKDWN PAETRDFIDA YLKEMSKHTG NPTSSFHEEN 310 320 330 340 350 360 LICSTLDLFF AGTETTSTTL RWALLYMALY PEIQEKVQAE IDRVIGQGQQ PSTAARESMP 370 380 390 400 410 420 YTNAVIHEVQ RMGNIIPLNV PREVTVDTTL AGYHLPKGTM ILTNLTALHR DPTEWATPDT 430 440 450 460 470 480 FNPDHFLENG QFKKREAFMP FSIGKRACLG EQLARTELFI FFTSLMQKFT FRPPNNEKLS 490 500 LKFRMGITIS PVSHRLCAVP QV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart." Wu S., Moomaw C.R., Tomer K.B., Falck J.R., Zeldin D.C. J. Biol. Chem. 271:3460-3468(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-20. Tissue: Liver.
[2]	Wu S., Zeldin D.C., Moomaw C.R., Tomer K.B., Falck J.R. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 339 AND 378.
[3]	"Involvement of CYP2J2 on the intestinal first-pass metabolism of antihistamine drug, astemizole." Matsumoto S., Hirama T., Matsubara T., Nagata K., Yamazoe Y. Drug Metab. Dispos. 30:1240-1245(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Cloning of CYP2J2 gene and identification of functional polymorphisms." King L.M., Ma J., Srettabunjong S., Graves J., Bradbury J.A., Li L., Spiecker M., Liao J.K., Mohrenweiser H., Zeldin D.C. Mol. Pharmacol. 61:840-852(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-143; CYS-158; ASN-192; ASN-342 AND TYR-404, CHARACTERIZATION OF VARIANTS.
[5]	NIEHS SNPs program Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-49.
[6]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Corpus callosum.
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Skin.
+	Additional computationally mapped references.

Web resources

Cytochrome P450 Allele Nomenclature Committee

CYP2J2 alleles

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	U37143 mRNA. Translation: AAC50370.2. AB080265 mRNA. Translation: BAB85489.1. AF272142 Genomic DNA. Translation: AAM44456.1. AY426985 Genomic DNA. Translation: AAQ93356.1. AK315387 mRNA. Translation: BAG37780.1. BC032594 mRNA. Translation: AAH32594.1.
RefSeq	NP_000766.2. NM_000775.2.
UniGene	Hs.152096.
3D structure databases
ProteinModelPortal	P51589.
SMR	P51589. Positions 44-474.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
BioGrid	107946. 2 interactions.
IntAct	P51589. 2 interactions.
STRING	9606.ENSP00000360247.
Chemistry
BindingDB	P51589.
ChEMBL	CHEMBL3491.
PTM databases
PhosphoSite	P51589.
Polymorphism databases
DMDM	21264413.
Proteomic databases
PaxDb	P51589.
PRIDE	P51589.
Protocols and materials databases
DNASU	1573.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000371204; ENSP00000360247; ENSG00000134716.
GeneID	1573.
KEGG	hsa:1573.
UCSC	uc001czq.3. human.
Organism-specific databases
CTD	1573.
GeneCards	GC01M060284.
HGNC	HGNC:2634. CYP2J2.
MIM	601258. gene.
neXtProt	NX_P51589.
PharmGKB	PA27112.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036991.
HOVERGEN	HBG015789.
InParanoid	P51589.
KO	K07418.
OMA	MSKHTGN.
OrthoDB	EOG7RBZ85.
PhylomeDB	P51589.
TreeFam	TF352043.
Enzyme and pathway databases
Reactome	REACT_111217. Metabolism.
Gene expression databases
ArrayExpress	P51589.
Bgee	P51589.
CleanEx	HS_CYP2J2.
Genevestigator	P51589.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008071. Cyt_P450_E_grp-I_CYP2J-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01688. EP450ICYP2J. PR00385. P450.
SUPFAM	SSF48264. SSF48264. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GeneWiki	CYP2J2.
GenomeRNAi	1573.
NextBio	35468234.
PRO	P51589.
SOURCE	Search...

Entry information

Entry name

CP2J2_HUMAN

Accession

Primary (citable) accession number: P51589
Secondary accession number(s): B2RD33, Q8TF13

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	May 27, 2002
Last modified:	April 16, 2014
This is version 134 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.