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P51589

- CP2J2_HUMAN

UniProt

P51589 - CP2J2_HUMAN

Protein

Cytochrome P450 2J2

Gene

CYP2J2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (27 May 2002)
      Previous versions | rss
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    Functioni

    This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi448 – 4481Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. arachidonic acid 11,12-epoxygenase activity Source: BHF-UCL
    2. arachidonic acid 14,15-epoxygenase activity Source: BHF-UCL
    3. arachidonic acid epoxygenase activity Source: BHF-UCL
    4. aromatase activity Source: UniProtKB-EC
    5. heme binding Source: InterPro
    6. iron ion binding Source: InterPro
    7. linoleic acid epoxygenase activity Source: BHF-UCL

    GO - Biological processi

    1. arachidonic acid metabolic process Source: Reactome
    2. epoxygenase P450 pathway Source: BHF-UCL
    3. icosanoid metabolic process Source: ProtInc
    4. linoleic acid metabolic process Source: BHF-UCL
    5. regulation of heart contraction Source: ProtInc
    6. small molecule metabolic process Source: Reactome
    7. xenobiotic metabolic process Source: BHF-UCL

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    ReactomeiREACT_13543. Xenobiotics.
    REACT_13814. Fatty acids.
    REACT_150417. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2J2 (EC:1.14.14.1)
    Alternative name(s):
    Arachidonic acid epoxygenase
    CYPIIJ2
    Gene namesi
    Name:CYP2J2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:2634. CYP2J2.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: Reactome

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA27112.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502Cytochrome P450 2J2PRO_0000051769Add
    BLAST

    Proteomic databases

    PaxDbiP51589.
    PRIDEiP51589.

    PTM databases

    PhosphoSiteiP51589.

    Expressioni

    Tissue specificityi

    Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.

    Gene expression databases

    ArrayExpressiP51589.
    BgeeiP51589.
    CleanExiHS_CYP2J2.
    GenevestigatoriP51589.

    Interactioni

    Protein-protein interaction databases

    BioGridi107946. 2 interactions.
    IntActiP51589. 2 interactions.
    STRINGi9606.ENSP00000360247.

    Structurei

    3D structure databases

    ProteinModelPortaliP51589.
    SMRiP51589. Positions 62-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036991.
    HOVERGENiHBG015789.
    InParanoidiP51589.
    KOiK07418.
    OMAiMSKHTGN.
    OrthoDBiEOG7RBZ85.
    PhylomeDBiP51589.
    TreeFamiTF352043.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008071. Cyt_P450_E_grp-I_CYP2J-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01688. EP450ICYP2J.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51589-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLAAMGSLAA ALWAVVHPRT LLLGTVAFLL AADFLKRRRP KNYPPGPWRL    50
    PFLGNFFLVD FEQSHLEVQL FVKKYGNLFS LELGDISAVL ITGLPLIKEA 100
    LIHMDQNFGN RPVTPMREHI FKKNGLIMSS GQAWKEQRRF TLTALRNFGL 150
    GKKSLEERIQ EEAQHLTEAI KEENGQPFDP HFKINNAVSN IICSITFGER 200
    FEYQDSWFQQ LLKLLDEVTY LEASKTCQLY NVFPWIMKFL PGPHQTLFSN 250
    WKKLKLFVSH MIDKHRKDWN PAETRDFIDA YLKEMSKHTG NPTSSFHEEN 300
    LICSTLDLFF AGTETTSTTL RWALLYMALY PEIQEKVQAE IDRVIGQGQQ 350
    PSTAARESMP YTNAVIHEVQ RMGNIIPLNV PREVTVDTTL AGYHLPKGTM 400
    ILTNLTALHR DPTEWATPDT FNPDHFLENG QFKKREAFMP FSIGKRACLG 450
    EQLARTELFI FFTSLMQKFT FRPPNNEKLS LKFRMGITIS PVSHRLCAVP 500
    QV 502
    Length:502
    Mass (Da):57,611
    Last modified:May 27, 2002 - v2
    Checksum:iB5F006581E249EC0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti264 – 2641K → E in BAG37780. (PubMed:14702039)Curated
    Sequence conflicti318 – 3181T → A in BAG37780. (PubMed:14702039)Curated
    Sequence conflicti339 – 3391A → V in BAB85489. (PubMed:12386130)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti49 – 491R → S.1 Publication
    Corresponds to variant rs11572190 [ dbSNP | Ensembl ].
    VAR_029159
    Natural varianti113 – 1131V → M.
    Corresponds to variant rs11572242 [ dbSNP | Ensembl ].
    VAR_029160
    Natural varianti124 – 1241N → S.
    Corresponds to variant rs2228113 [ dbSNP | Ensembl ].
    VAR_022084
    Natural varianti143 – 1431T → A in allele CYP2J2*2; significantly reduced metabolism of both arachidonic acid and linoleic acid. 1 Publication
    Corresponds to variant rs55753213 [ dbSNP | Ensembl ].
    VAR_014317
    Natural varianti158 – 1581R → C in allele CYP2J2*3; significantly reduced metabolism of both arachidonic acid and linoleic acid. 1 Publication
    Corresponds to variant rs56307989 [ dbSNP | Ensembl ].
    VAR_014318
    Natural varianti192 – 1921I → N in allele CYP2J2*4; significantly reduced metabolism of arachidonic acid only. 1 Publication
    Corresponds to variant rs66515830 [ dbSNP | Ensembl ].
    VAR_014319
    Natural varianti342 – 3421D → N in allele CYP2J2*5; no change in activity. 1 Publication
    Corresponds to variant rs56053398 [ dbSNP | Ensembl ].
    VAR_014320
    Natural varianti404 – 4041N → Y in allele CYP2J2*6; significantly reduced metabolism of both arachidonic acid and linoleic acid. 1 Publication
    VAR_014321

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37143 mRNA. Translation: AAC50370.2.
    AB080265 mRNA. Translation: BAB85489.1.
    AF272142 Genomic DNA. Translation: AAM44456.1.
    AY426985 Genomic DNA. Translation: AAQ93356.1.
    AK315387 mRNA. Translation: BAG37780.1.
    BC032594 mRNA. Translation: AAH32594.1.
    CCDSiCCDS613.1.
    RefSeqiNP_000766.2. NM_000775.2.
    UniGeneiHs.152096.

    Genome annotation databases

    EnsembliENST00000371204; ENSP00000360247; ENSG00000134716.
    GeneIDi1573.
    KEGGihsa:1573.
    UCSCiuc001czq.3. human.

    Polymorphism databases

    DMDMi21264413.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    Cytochrome P450 Allele Nomenclature Committee

    CYP2J2 alleles

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U37143 mRNA. Translation: AAC50370.2 .
    AB080265 mRNA. Translation: BAB85489.1 .
    AF272142 Genomic DNA. Translation: AAM44456.1 .
    AY426985 Genomic DNA. Translation: AAQ93356.1 .
    AK315387 mRNA. Translation: BAG37780.1 .
    BC032594 mRNA. Translation: AAH32594.1 .
    CCDSi CCDS613.1.
    RefSeqi NP_000766.2. NM_000775.2.
    UniGenei Hs.152096.

    3D structure databases

    ProteinModelPortali P51589.
    SMRi P51589. Positions 62-498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107946. 2 interactions.
    IntActi P51589. 2 interactions.
    STRINGi 9606.ENSP00000360247.

    Chemistry

    BindingDBi P51589.
    ChEMBLi CHEMBL3491.

    PTM databases

    PhosphoSitei P51589.

    Polymorphism databases

    DMDMi 21264413.

    Proteomic databases

    PaxDbi P51589.
    PRIDEi P51589.

    Protocols and materials databases

    DNASUi 1573.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000371204 ; ENSP00000360247 ; ENSG00000134716 .
    GeneIDi 1573.
    KEGGi hsa:1573.
    UCSCi uc001czq.3. human.

    Organism-specific databases

    CTDi 1573.
    GeneCardsi GC01M060284.
    HGNCi HGNC:2634. CYP2J2.
    MIMi 601258. gene.
    neXtProti NX_P51589.
    PharmGKBi PA27112.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036991.
    HOVERGENi HBG015789.
    InParanoidi P51589.
    KOi K07418.
    OMAi MSKHTGN.
    OrthoDBi EOG7RBZ85.
    PhylomeDBi P51589.
    TreeFami TF352043.

    Enzyme and pathway databases

    Reactomei REACT_13543. Xenobiotics.
    REACT_13814. Fatty acids.
    REACT_150417. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).

    Miscellaneous databases

    GeneWikii CYP2J2.
    GenomeRNAii 1573.
    NextBioi 35468234.
    PROi P51589.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P51589.
    Bgeei P51589.
    CleanExi HS_CYP2J2.
    Genevestigatori P51589.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008071. Cyt_P450_E_grp-I_CYP2J-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01688. EP450ICYP2J.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart."
      Wu S., Moomaw C.R., Tomer K.B., Falck J.R., Zeldin D.C.
      J. Biol. Chem. 271:3460-3468(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-20.
      Tissue: Liver.
    2. Wu S., Zeldin D.C., Moomaw C.R., Tomer K.B., Falck J.R.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 339 AND 378.
    3. "Involvement of CYP2J2 on the intestinal first-pass metabolism of antihistamine drug, astemizole."
      Matsumoto S., Hirama T., Matsubara T., Nagata K., Yamazoe Y.
      Drug Metab. Dispos. 30:1240-1245(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-143; CYS-158; ASN-192; ASN-342 AND TYR-404, CHARACTERIZATION OF VARIANTS.
    5. NIEHS SNPs program
      Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-49.
    6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Corpus callosum.
    7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Skin.

    Entry informationi

    Entry nameiCP2J2_HUMAN
    AccessioniPrimary (citable) accession number: P51589
    Secondary accession number(s): B2RD33, Q8TF13
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 27, 2002
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3