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Protein

Cytochrome P450 2J2

Gene

CYP2J2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi448 – 4481Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. arachidonic acid 11,12-epoxygenase activity Source: BHF-UCL
  2. arachidonic acid 14,15-epoxygenase activity Source: BHF-UCL
  3. arachidonic acid epoxygenase activity Source: BHF-UCL
  4. aromatase activity Source: UniProtKB-EC
  5. heme binding Source: GO_Central
  6. iron ion binding Source: InterPro
  7. linoleic acid epoxygenase activity Source: BHF-UCL
  8. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen Source: GO_Central
  9. oxygen binding Source: GO_Central
  10. steroid hydroxylase activity Source: GO_Central

GO - Biological processi

  1. arachidonic acid metabolic process Source: Reactome
  2. epoxygenase P450 pathway Source: BHF-UCL
  3. exogenous drug catabolic process Source: GO_Central
  4. icosanoid metabolic process Source: ProtInc
  5. linoleic acid metabolic process Source: BHF-UCL
  6. oxidation-reduction process Source: GO_Central
  7. regulation of heart contraction Source: ProtInc
  8. small molecule metabolic process Source: Reactome
  9. xenobiotic metabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.159. 2681.
ReactomeiREACT_13543. Xenobiotics.
REACT_13814. Fatty acids.
REACT_150417. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2J2 (EC:1.14.14.1)
Alternative name(s):
Arachidonic acid epoxygenase
CYPIIJ2
Gene namesi
Name:CYP2J2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:2634. CYP2J2.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: GO_Central
  2. endoplasmic reticulum membrane Source: Reactome
  3. extracellular vesicular exosome Source: UniProtKB
  4. intracellular membrane-bounded organelle Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27112.

Chemistry

DrugBankiDB06605. Apixaban.
DB00637. Astemizole.
DB00169. Cholecalciferol.
DB08918. Levomilnacipran.
DB00179. Masoprocol.
DB06228. Rivaroxaban.

Polymorphism and mutation databases

BioMutaiCYP2J2.
DMDMi21264413.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502Cytochrome P450 2J2PRO_0000051769Add
BLAST

Proteomic databases

MaxQBiP51589.
PaxDbiP51589.
PRIDEiP51589.

PTM databases

PhosphoSiteiP51589.

Expressioni

Tissue specificityi

Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.

Gene expression databases

BgeeiP51589.
CleanExiHS_CYP2J2.
ExpressionAtlasiP51589. baseline and differential.
GenevestigatoriP51589.

Interactioni

Protein-protein interaction databases

BioGridi107946. 3 interactions.
IntActiP51589. 2 interactions.
STRINGi9606.ENSP00000360247.

Structurei

3D structure databases

ProteinModelPortaliP51589.
SMRiP51589. Positions 62-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiP51589.
KOiK07418.
OMAiMSKHTGN.
OrthoDBiEOG7RBZ85.
PhylomeDBiP51589.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008071. Cyt_P450_E_grp-I_CYP2J-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01688. EP450ICYP2J.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51589-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLAAMGSLAA ALWAVVHPRT LLLGTVAFLL AADFLKRRRP KNYPPGPWRL
60 70 80 90 100
PFLGNFFLVD FEQSHLEVQL FVKKYGNLFS LELGDISAVL ITGLPLIKEA
110 120 130 140 150
LIHMDQNFGN RPVTPMREHI FKKNGLIMSS GQAWKEQRRF TLTALRNFGL
160 170 180 190 200
GKKSLEERIQ EEAQHLTEAI KEENGQPFDP HFKINNAVSN IICSITFGER
210 220 230 240 250
FEYQDSWFQQ LLKLLDEVTY LEASKTCQLY NVFPWIMKFL PGPHQTLFSN
260 270 280 290 300
WKKLKLFVSH MIDKHRKDWN PAETRDFIDA YLKEMSKHTG NPTSSFHEEN
310 320 330 340 350
LICSTLDLFF AGTETTSTTL RWALLYMALY PEIQEKVQAE IDRVIGQGQQ
360 370 380 390 400
PSTAARESMP YTNAVIHEVQ RMGNIIPLNV PREVTVDTTL AGYHLPKGTM
410 420 430 440 450
ILTNLTALHR DPTEWATPDT FNPDHFLENG QFKKREAFMP FSIGKRACLG
460 470 480 490 500
EQLARTELFI FFTSLMQKFT FRPPNNEKLS LKFRMGITIS PVSHRLCAVP

QV
Length:502
Mass (Da):57,611
Last modified:May 27, 2002 - v2
Checksum:iB5F006581E249EC0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti264 – 2641K → E in BAG37780 (PubMed:14702039).Curated
Sequence conflicti318 – 3181T → A in BAG37780 (PubMed:14702039).Curated
Sequence conflicti339 – 3391A → V in BAB85489 (PubMed:12386130).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti49 – 491R → S.1 Publication
Corresponds to variant rs11572190 [ dbSNP | Ensembl ].
VAR_029159
Natural varianti113 – 1131V → M.
Corresponds to variant rs11572242 [ dbSNP | Ensembl ].
VAR_029160
Natural varianti124 – 1241N → S.
Corresponds to variant rs2228113 [ dbSNP | Ensembl ].
VAR_022084
Natural varianti143 – 1431T → A in allele CYP2J2*2; significantly reduced metabolism of both arachidonic acid and linoleic acid. 1 Publication
Corresponds to variant rs55753213 [ dbSNP | Ensembl ].
VAR_014317
Natural varianti158 – 1581R → C in allele CYP2J2*3; significantly reduced metabolism of both arachidonic acid and linoleic acid. 1 Publication
Corresponds to variant rs56307989 [ dbSNP | Ensembl ].
VAR_014318
Natural varianti192 – 1921I → N in allele CYP2J2*4; significantly reduced metabolism of arachidonic acid only. 1 Publication
Corresponds to variant rs66515830 [ dbSNP | Ensembl ].
VAR_014319
Natural varianti342 – 3421D → N in allele CYP2J2*5; no change in activity. 1 Publication
Corresponds to variant rs56053398 [ dbSNP | Ensembl ].
VAR_014320
Natural varianti404 – 4041N → Y in allele CYP2J2*6; significantly reduced metabolism of both arachidonic acid and linoleic acid. 1 Publication
VAR_014321

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37143 mRNA. Translation: AAC50370.2.
AB080265 mRNA. Translation: BAB85489.1.
AF272142 Genomic DNA. Translation: AAM44456.1.
AY426985 Genomic DNA. Translation: AAQ93356.1.
AK315387 mRNA. Translation: BAG37780.1.
BC032594 mRNA. Translation: AAH32594.1.
CCDSiCCDS613.1.
RefSeqiNP_000766.2. NM_000775.2.
UniGeneiHs.152096.

Genome annotation databases

EnsembliENST00000371204; ENSP00000360247; ENSG00000134716.
GeneIDi1573.
KEGGihsa:1573.
UCSCiuc001czq.3. human.

Polymorphism and mutation databases

BioMutaiCYP2J2.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

Cytochrome P450 Allele Nomenclature Committee

CYP2J2 alleles

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U37143 mRNA. Translation: AAC50370.2.
AB080265 mRNA. Translation: BAB85489.1.
AF272142 Genomic DNA. Translation: AAM44456.1.
AY426985 Genomic DNA. Translation: AAQ93356.1.
AK315387 mRNA. Translation: BAG37780.1.
BC032594 mRNA. Translation: AAH32594.1.
CCDSiCCDS613.1.
RefSeqiNP_000766.2. NM_000775.2.
UniGeneiHs.152096.

3D structure databases

ProteinModelPortaliP51589.
SMRiP51589. Positions 62-498.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107946. 3 interactions.
IntActiP51589. 2 interactions.
STRINGi9606.ENSP00000360247.

Chemistry

BindingDBiP51589.
ChEMBLiCHEMBL3491.
DrugBankiDB06605. Apixaban.
DB00637. Astemizole.
DB00169. Cholecalciferol.
DB08918. Levomilnacipran.
DB00179. Masoprocol.
DB06228. Rivaroxaban.

PTM databases

PhosphoSiteiP51589.

Polymorphism and mutation databases

BioMutaiCYP2J2.
DMDMi21264413.

Proteomic databases

MaxQBiP51589.
PaxDbiP51589.
PRIDEiP51589.

Protocols and materials databases

DNASUi1573.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371204; ENSP00000360247; ENSG00000134716.
GeneIDi1573.
KEGGihsa:1573.
UCSCiuc001czq.3. human.

Organism-specific databases

CTDi1573.
GeneCardsiGC01M060284.
HGNCiHGNC:2634. CYP2J2.
MIMi601258. gene.
neXtProtiNX_P51589.
PharmGKBiPA27112.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiP51589.
KOiK07418.
OMAiMSKHTGN.
OrthoDBiEOG7RBZ85.
PhylomeDBiP51589.
TreeFamiTF352043.

Enzyme and pathway databases

BRENDAi1.14.13.159. 2681.
ReactomeiREACT_13543. Xenobiotics.
REACT_13814. Fatty acids.
REACT_150417. Synthesis of epoxy (EET) and dihydroxyeicosatrienoic acids (DHET).

Miscellaneous databases

GeneWikiiCYP2J2.
GenomeRNAii1573.
NextBioi35468234.
PROiP51589.
SOURCEiSearch...

Gene expression databases

BgeeiP51589.
CleanExiHS_CYP2J2.
ExpressionAtlasiP51589. baseline and differential.
GenevestigatoriP51589.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008071. Cyt_P450_E_grp-I_CYP2J-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01688. EP450ICYP2J.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and expression of CYP2J2, a human cytochrome P450 arachidonic acid epoxygenase highly expressed in heart."
    Wu S., Moomaw C.R., Tomer K.B., Falck J.R., Zeldin D.C.
    J. Biol. Chem. 271:3460-3468(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-20.
    Tissue: Liver.
  2. Wu S., Zeldin D.C., Moomaw C.R., Tomer K.B., Falck J.R.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 339 AND 378.
  3. "Involvement of CYP2J2 on the intestinal first-pass metabolism of antihistamine drug, astemizole."
    Matsumoto S., Hirama T., Matsubara T., Nagata K., Yamazoe Y.
    Drug Metab. Dispos. 30:1240-1245(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS ALA-143; CYS-158; ASN-192; ASN-342 AND TYR-404, CHARACTERIZATION OF VARIANTS.
  5. NIEHS SNPs program
    Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT SER-49.
  6. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Corpus callosum.
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiCP2J2_HUMAN
AccessioniPrimary (citable) accession number: P51589
Secondary accession number(s): B2RD33, Q8TF13
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 27, 2002
Last modified: April 29, 2015
This is version 146 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.