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P51588

- TRYP_SARBU

UniProt

P51588 - TRYP_SARBU

Protein

Trypsin

Gene
N/A
Organism
Sarcophaga bullata (Grey flesh fly) (Neobellieria bullata)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in digestion of a protein meal.

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei68 – 681Charge relay systemBy similarity
    Active sitei113 – 1131Charge relay systemBy similarity
    Sitei202 – 2021Required for specificityBy similarity
    Active sitei208 – 2081Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. digestion Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Protein family/group databases

    MEROPSiS01.A85.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Trypsin (EC:3.4.21.4)
    OrganismiSarcophaga bullata (Grey flesh fly) (Neobellieria bullata)
    Taxonomic identifieri7385 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaOestroideaSarcophagidaeNeobellieria

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1616Sequence AnalysisAdd
    BLAST
    Propeptidei17 – 2610Activation peptide1 PublicationPRO_0000028297
    Chaini27 – 254228TrypsinPRO_0000028298Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi53 ↔ 69PROSITE-ProRule annotation
    Disulfide bondi154 ↔ 158PROSITE-ProRule annotation
    Disulfide bondi178 ↔ 195PROSITE-ProRule annotation
    Disulfide bondi204 ↔ 228PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliP51588.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 252226Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P51588-1 [UniParc]FASTAAdd to Basket

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    MLRFIAVFAL VNCALAGTLP NDLDGRIVNG VDTTIEAHPY QVPLQNAALS    50
    HFCGGSIISE DLVVTAAHCM QSYTASQIKV RLGSTIYNEG GELVSVKAFK 100
    FHEGYNPKTM VNDVALIKLA TPVRESSKIR YIRLADRTPP TGTPAVVTGW 150
    GTKCFLTCVS LPKTLQEVEV DIVDQKACAS NEFKYGSQIQ DTMVCAYALK 200
    KDACQGDSGG PLVANNQLVG IVSWGSGCAR VGYPGVFCDV PSVRSWIEKT 250
    AKEL 254
    Length:254
    Mass (Da):27,309
    Last modified:October 1, 1996 - v1
    Checksum:iF07542C0EC1452AE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94691 mRNA. Translation: CAA64354.1.
    PIRiS65465.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94691 mRNA. Translation: CAA64354.1 .
    PIRi S65465.

    3D structure databases

    ProteinModelPortali P51588.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.A85.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular sequencing and modeling of Neobellieria bullata trypsin. Evidence for translational control by Neobellieria trypsin-modulating oostatic factor."
      Borovsky D., Janssen I., Vanden Broeck J., Huybrechts R., Verhaert P., de Bondt H.L., Bylemans D., de Loof A.
      Eur. J. Biochem. 237:279-287(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-36, 3D-STRUCTURE MODELING.
      Tissue: Gut.

    Entry informationi

    Entry nameiTRYP_SARBU
    AccessioniPrimary (citable) accession number: P51588
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3