P51588 (TRYP_SARBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Trypsin EC=3.4.21.4
Organism	Sarcophaga bullata (Grey flesh fly) (Neobellieria bullata)
Taxonomic identifier	7385 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Calyptratae › Oestroidea › Sarcophagidae › Sarcophaginae › Neobellieria

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in digestion of a protein meal.
Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Subcellular location	Secreted › extracellular space.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Propeptide	17 – 26	10	Activation peptide		PRO_0000028297
	Chain	27 – 254	228	Trypsin		PRO_0000028298
Regions
	Domain	27 – 252	226	Peptidase S1
Sites
	Active site	68	1	Charge relay system By similarity
	Active site	113	1	Charge relay system By similarity
	Active site	208	1	Charge relay system By similarity
	Site	202	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	53 ↔ 69		By similarity
	Disulfide bond	154 ↔ 158		By similarity
	Disulfide bond	178 ↔ 195		By similarity
	Disulfide bond	204 ↔ 228		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51588 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F07542C0EC1452AE	FASTA	254	27,309
	10 20 30 40 50 60 MLRFIAVFAL VNCALAGTLP NDLDGRIVNG VDTTIEAHPY QVPLQNAALS HFCGGSIISE 70 80 90 100 110 120 DLVVTAAHCM QSYTASQIKV RLGSTIYNEG GELVSVKAFK FHEGYNPKTM VNDVALIKLA 130 140 150 160 170 180 TPVRESSKIR YIRLADRTPP TGTPAVVTGW GTKCFLTCVS LPKTLQEVEV DIVDQKACAS 190 200 210 220 230 240 NEFKYGSQIQ DTMVCAYALK KDACQGDSGG PLVANNQLVG IVSWGSGCAR VGYPGVFCDV 250 PSVRSWIEKT AKEL

References

[1]

"Molecular sequencing and modeling of Neobellieria bullata trypsin. Evidence for translational control by Neobellieria trypsin-modulating oostatic factor." Borovsky D., Janssen I., Vanden Broeck J., Huybrechts R., Verhaert P., de Bondt H.L., Bylemans D., de Loof A. Eur. J. Biochem. 237:279-287(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 27-36, 3D-STRUCTURE MODELING. Tissue: Gut.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X94691 mRNA. Translation: CAA64354.1.
PIR	S65465.
3D structure databases
ProteinModelPortal	P51588.
ModBase	Search...
Protein family/group databases
MEROPS	S01.A85.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRYP_SARBU

Accession

Primary (citable) accession number: P51588

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)