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P51585 (ALGD_AZOVI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
GDP-mannose 6-dehydrogenase
Short name=GMD
EC=1.1.1.132
Gene names
Name:algD
OrganismAzotobacter vinelandii
Taxonomic identifier354 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and is essential for cyst formation.

Catalytic activity

GDP-D-mannose + 2 NAD+ + H2O = GDP-D-mannuronate + 2 NADH.

Pathway

Glycan biosynthesis; alginate biosynthesis.

Sequence similarities

Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
   Biological processAlginate biosynthesis
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processalginic acid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionGDP-mannose 6-dehydrogenase activity

Inferred from electronic annotation. Source: EC

NAD binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 436436GDP-mannose 6-dehydrogenase
PRO_0000074066

Sites

Active site2681 By similarity
Site101NAD binding By similarity
Site111NAD binding By similarity
Site301NAD binding By similarity
Site351NAD binding By similarity
Site861NAD binding By similarity
Site1611NAD binding By similarity
Site2711NAD binding By similarity
Site3311NAD binding By similarity

Sequences

Sequence LengthMass (Da)Tools
P51585 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: FC9E49347F618677

FASTA43647,003
        10         20         30         40         50         60 
MRISIFGLGY VGAVCAGCLS GRGHEVVGVD ISAAKIDMIN QGKSPIVEPG LGELLAEGVK 

        70         80         90        100        110        120 
TGRLRGTTNV TEAVLATELS MLCVGTPSKL NGDLELDYIE EVCRQMGSAL RDKTERHTVV 

       130        140        150        160        170        180 
VRSTVLPGTV HNVVIPILEE FSGKKAGVDF GVAVNPEFLR ESTAIKDYNF PPMTVIGELD 

       190        200        210        220        230        240 
KASGRRLASI YAELDAPIVR KGIAVAEMIK YTCNVWHATK VTFANEIGNI AKAAGVDGRE 

       250        260        270        280        290        300 
VMEVVCMDNK LNLSQYYMRP GLAFGGSCLP KDVSALSYRA HLWDIEAPLI SSLMRSNAAQ 

       310        320        330        340        350        360 
VQKAYDMIDK HGSRKVALLG LSFKAGTDDL RESPQLELAE MLIGKGFKLS IFDSNVEYAR 

       370        380        390        400        410        420 
DHGANGHYIK NEIPHVSALL QSDLDKVVAE ADVIVLGNAD PRFEKLAKDV PAGKKVIDLV 

       430 
GFMPQRTAGA AEGICW

« Hide

References

[1]"Characterization of the gene coding for GDP-mannose dehydrogenase (algD) from Azotobacter vinelandii."
Campos M., Martinez-Salazar J.M., Lloret L., Moreno S., Nunez C., Espin G., Soberon-Chavez G.
J. Bacteriol. 178:1793-1799(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 9046.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U11240 Unassigned DNA. Translation: AAB01487.1.

3D structure databases

ProteinModelPortalP51585.
SMRP51585. Positions 1-436.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00286.

Family and domain databases

Gene3D3.40.50.720. 2 hits.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. Nucleotide_sugar_DH.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PfamPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFPIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMSSF48179. 6DGDH_C_like. 1 hit.
SSF52413. UDP-Glc/GDP-Man_DH_C. 1 hit.
TIGRFAMsTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetSearch...

Entry information

Entry nameALGD_AZOVI
AccessionPrimary (citable) accession number: P51585
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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