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Protein

Multifunctional protein ADE2

Gene

Paics

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Multifunctional protein ADE2 (Paics)
  2. Adenylosuccinate lyase (Adsl)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Multifunctional protein ADE2 (Paics)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101For SAICAR synthetase activityBy similarity1
Active sitei107For SAICAR synthetase activityBy similarity1
Active sitei215For SAICAR synthetase activityBy similarity1
Active sitei303For AIR carboxylase activityBy similarity1
Active sitei332For AIR carboxylase activityBy similarity1
Sitei334Essential for AIR carboxylase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  • G1/S transition of mitotic cell cycle Source: RGD

Keywordsi

Molecular functionDecarboxylase, Ligase, Lyase, Multifunctional enzyme
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00130
UPA00074; UER00131

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional protein ADE2
Including the following 2 domains:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Phosphoribosylaminoimidazole carboxylase (EC:4.1.1.21)
Alternative name(s):
AIR carboxylase
Short name:
AIRC
Gene namesi
Name:Paics
Synonyms:Ade2, Airc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi620066 Paics

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000750332 – 425Multifunctional protein ADE2Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei22PhosphotyrosineBy similarity1
Modified residuei27PhosphoserineCombined sources1
Modified residuei36N6-acetyllysineBy similarity1
Modified residuei107PhosphoserineBy similarity1
Modified residuei238PhosphothreonineBy similarity1
Modified residuei247N6-acetyllysineBy similarity1
Modified residuei274PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP51583
PRIDEiP51583

PTM databases

iPTMnetiP51583
PhosphoSitePlusiP51583

Expressioni

Gene expression databases

BgeeiENSRNOG00000002101
GenevisibleiP51583 RN

Interactioni

Subunit structurei

Homooctamer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP51583, 1 interactor
STRINGi10116.ENSRNOP00000061495

Structurei

3D structure databases

ProteinModelPortaliP51583
SMRiP51583
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 260SAICAR synthetaseAdd BLAST259
Regioni261 – 425AIR carboxylaseAdd BLAST165
Regioni261 – 266LinkerBy similarity6
Regioni267 – 425AIR carboxylaseBy similarityAdd BLAST159

Sequence similaritiesi

In the N-terminal section; belongs to the SAICAR synthetase family.Curated
In the C-terminal section; belongs to the AIR carboxylase family. Class II subfamily.Curated

Phylogenomic databases

eggNOGiKOG2835 Eukaryota
COG0041 LUCA
COG0152 LUCA
GeneTreeiENSGT00390000010172
HOGENOMiHOG000082628
HOVERGENiHBG008335
InParanoidiP51583
KOiK01587
OMAiWSDEQII
OrthoDBiEOG091G08DN
PhylomeDBiP51583
TreeFamiTF106384

Family and domain databases

Gene3Di3.40.50.7700, 1 hit
HAMAPiMF_02045 PurE_classII, 1 hit
MF_00137 SAICAR_synth, 1 hit
InterProiView protein in InterPro
IPR033626 PurE_classII
IPR000031 PurE_dom
IPR035893 PurE_sf
IPR028923 SAICAR_synt/ADE2_N
IPR018236 SAICAR_synthetase_CS
PfamiView protein in Pfam
PF00731 AIRC, 1 hit
PF01259 SAICAR_synt, 1 hit
SMARTiView protein in SMART
SM01001 AIRC, 1 hit
SUPFAMiSSF52255 SSF52255, 1 hit
TIGRFAMsiTIGR01162 purE, 1 hit
PROSITEiView protein in PROSITE
PS01057 SAICAR_SYNTHETASE_1, 1 hit
PS01058 SAICAR_SYNTHETASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAEVLNIG RKLYEGKTKE VYELLDSPGR VLLQSKDQIT AGNAARKNHL
60 70 80 90 100
EGKAAISNKI TSCIFQLLQE AGIKTAFTKK CGETAFIAPQ CEMIPIEWVC
110 120 130 140 150
RRIATGSFLK RNPGVKEGYR FYPPKVEMFF KDDANNDPQW SEEQLIAAKF
160 170 180 190 200
CFAGLVIGQT EVDIMSHATQ AIFEILEKSW LPQNCTLVDM KIEFGVDVTT
210 220 230 240 250
KEIVLADVID NDSWRLWPSG DRSQQKDKQS YRDLKEVTPE GLQMVKKNFE
260 270 280 290 300
WVADRVELLL KSNSQCRVVV LMGSTSDLGH CEKIKKACGN FGIPCELRVT
310 320 330 340 350
SAHKGPDETL RIKAEYEGDG IPTVFVAVAG RSNGLGPVMS GNTAYPVISC
360 370 380 390 400
PPITADWGAQ DVWSSLRLPS GIGCSTILSP EGSAQFAAQI FGLNNHLVWA
410 420
KLRASKLNTW ISLKQADKKI RECNL
Length:425
Mass (Da):47,096
Last modified:January 23, 2007 - v3
Checksum:i8E097CFD114E9DAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37979 mRNA Translation: BAA07197.1
BC072508 mRNA Translation: AAH72508.1
BC085711 mRNA Translation: AAH85711.1
PIRiS55684
RefSeqiNP_543186.1, NM_080910.3
XP_006250914.1, XM_006250852.3
XP_006250915.1, XM_006250853.3
XP_006250916.1, XM_006250854.2
UniGeneiRn.3015

Genome annotation databases

EnsembliENSRNOT00000063942; ENSRNOP00000061495; ENSRNOG00000002101
GeneIDi140946
KEGGirno:140946
UCSCiRGD:620066 rat

Similar proteinsi

Entry informationi

Entry nameiPUR6_RAT
AccessioniPrimary (citable) accession number: P51583
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 132 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health