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Protein

Multifunctional protein ADE2

Gene

Paics

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Multifunctional protein ADE2 (Paics)
  2. Adenylosuccinate lyase (Adsl)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Multifunctional protein ADE2 (Paics)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei101 – 1011For SAICAR synthetase activityBy similarity
Active sitei107 – 1071For SAICAR synthetase activityBy similarity
Active sitei215 – 2151For SAICAR synthetase activityBy similarity
Active sitei303 – 3031For AIR carboxylase activityBy similarity
Active sitei332 – 3321For AIR carboxylase activityBy similarity
Sitei334 – 3341Essential for AIR carboxylase activityBy similarity

GO - Molecular functioni

GO - Biological processi

  • 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  • G1/S transition of mitotic cell cycle Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Ligase, Lyase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-73817. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00130.
UPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional protein ADE2
Including the following 2 domains:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Phosphoribosylaminoimidazole carboxylase (EC:4.1.1.21)
Alternative name(s):
AIR carboxylase
Short name:
AIRC
Gene namesi
Name:Paics
Synonyms:Ade2, Airc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi620066. Paics.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 425424Multifunctional protein ADE2PRO_0000075033Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei22 – 221PhosphotyrosineBy similarity
Modified residuei27 – 271PhosphoserineCombined sources
Modified residuei36 – 361N6-acetyllysineBy similarity
Modified residuei107 – 1071PhosphoserineBy similarity
Modified residuei238 – 2381PhosphothreonineBy similarity
Modified residuei247 – 2471N6-acetyllysineBy similarity
Modified residuei274 – 2741PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP51583.
PRIDEiP51583.

PTM databases

iPTMnetiP51583.

Expressioni

Gene expression databases

GenevisibleiP51583. RN.

Interactioni

Subunit structurei

Homooctamer.By similarity

Protein-protein interaction databases

IntActiP51583. 1 interaction.
MINTiMINT-4566100.
STRINGi10116.ENSRNOP00000061495.

Structurei

3D structure databases

ProteinModelPortaliP51583.
SMRiP51583. Positions 7-425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 260259SAICAR synthetaseAdd
BLAST
Regioni261 – 425165AIR carboxylaseAdd
BLAST
Regioni261 – 2666LinkerBy similarity
Regioni267 – 425159AIR carboxylaseBy similarityAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the SAICAR synthetase family.Curated
In the C-terminal section; belongs to the AIR carboxylase family. Class II subfamily.Curated

Phylogenomic databases

eggNOGiKOG2835. Eukaryota.
COG0041. LUCA.
COG0152. LUCA.
GeneTreeiENSGT00390000010172.
HOGENOMiHOG000082628.
HOVERGENiHBG008335.
InParanoidiP51583.
KOiK01587.
OMAiWQTKNCA.
OrthoDBiEOG725DHT.
PhylomeDBiP51583.
TreeFamiTF106384.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.7700. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
MF_02045. PurE_classII.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR000031. PurE_dom.
IPR028923. SAICAR_synt/ADE2_N.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF00731. AIRC. 1 hit.
PF01259. SAICAR_synt. 1 hit.
[Graphical view]
SMARTiSM01001. AIRC. 1 hit.
[Graphical view]
SUPFAMiSSF52255. SSF52255. 1 hit.
TIGRFAMsiTIGR01162. purE. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51583-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAEVLNIG RKLYEGKTKE VYELLDSPGR VLLQSKDQIT AGNAARKNHL
60 70 80 90 100
EGKAAISNKI TSCIFQLLQE AGIKTAFTKK CGETAFIAPQ CEMIPIEWVC
110 120 130 140 150
RRIATGSFLK RNPGVKEGYR FYPPKVEMFF KDDANNDPQW SEEQLIAAKF
160 170 180 190 200
CFAGLVIGQT EVDIMSHATQ AIFEILEKSW LPQNCTLVDM KIEFGVDVTT
210 220 230 240 250
KEIVLADVID NDSWRLWPSG DRSQQKDKQS YRDLKEVTPE GLQMVKKNFE
260 270 280 290 300
WVADRVELLL KSNSQCRVVV LMGSTSDLGH CEKIKKACGN FGIPCELRVT
310 320 330 340 350
SAHKGPDETL RIKAEYEGDG IPTVFVAVAG RSNGLGPVMS GNTAYPVISC
360 370 380 390 400
PPITADWGAQ DVWSSLRLPS GIGCSTILSP EGSAQFAAQI FGLNNHLVWA
410 420
KLRASKLNTW ISLKQADKKI RECNL
Length:425
Mass (Da):47,096
Last modified:January 23, 2007 - v3
Checksum:i8E097CFD114E9DAB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37979 mRNA. Translation: BAA07197.1.
BC072508 mRNA. Translation: AAH72508.1.
BC085711 mRNA. Translation: AAH85711.1.
PIRiS55684.
RefSeqiNP_543186.1. NM_080910.3.
XP_006250914.1. XM_006250852.2.
XP_006250915.1. XM_006250853.2.
XP_006250916.1. XM_006250854.2.
UniGeneiRn.3015.

Genome annotation databases

EnsembliENSRNOT00000063942; ENSRNOP00000061495; ENSRNOG00000002101.
GeneIDi140946.
KEGGirno:140946.
UCSCiRGD:620066. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D37979 mRNA. Translation: BAA07197.1.
BC072508 mRNA. Translation: AAH72508.1.
BC085711 mRNA. Translation: AAH85711.1.
PIRiS55684.
RefSeqiNP_543186.1. NM_080910.3.
XP_006250914.1. XM_006250852.2.
XP_006250915.1. XM_006250853.2.
XP_006250916.1. XM_006250854.2.
UniGeneiRn.3015.

3D structure databases

ProteinModelPortaliP51583.
SMRiP51583. Positions 7-425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51583. 1 interaction.
MINTiMINT-4566100.
STRINGi10116.ENSRNOP00000061495.

PTM databases

iPTMnetiP51583.

Proteomic databases

PaxDbiP51583.
PRIDEiP51583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000063942; ENSRNOP00000061495; ENSRNOG00000002101.
GeneIDi140946.
KEGGirno:140946.
UCSCiRGD:620066. rat.

Organism-specific databases

CTDi10606.
RGDi620066. Paics.

Phylogenomic databases

eggNOGiKOG2835. Eukaryota.
COG0041. LUCA.
COG0152. LUCA.
GeneTreeiENSGT00390000010172.
HOGENOMiHOG000082628.
HOVERGENiHBG008335.
InParanoidiP51583.
KOiK01587.
OMAiWQTKNCA.
OrthoDBiEOG725DHT.
PhylomeDBiP51583.
TreeFamiTF106384.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00130.
UPA00074; UER00131.
ReactomeiR-RNO-73817. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

NextBioi620860.
PROiP51583.

Gene expression databases

GenevisibleiP51583. RN.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
3.40.50.7700. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
MF_02045. PurE_classII.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR000031. PurE_dom.
IPR028923. SAICAR_synt/ADE2_N.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF00731. AIRC. 1 hit.
PF01259. SAICAR_synt. 1 hit.
[Graphical view]
SMARTiSM01001. AIRC. 1 hit.
[Graphical view]
SUPFAMiSSF52255. SSF52255. 1 hit.
TIGRFAMsiTIGR01162. purE. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rat genomic structure of amidophosphoribosyltransferase, cDNA sequence of aminoimidazole ribonucleotide carboxylase, and cell cycle-dependent expression of these two physically linked genes."
    Iwahana H., Honda S., Tsujisawa T., Takahashi Y., Adzuma K., Katashima R., Yamaoka T., Moritani M., Yoshimoto K., Itakura M.
    Biochim. Biophys. Acta 1261:369-380(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPUR6_RAT
AccessioniPrimary (citable) accession number: P51583
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: May 11, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.