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P51583 (PUR6_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Multifunctional protein ADE2

Including the following 2 domains:

  1. Phosphoribosylaminoimidazole-succinocarboxamide synthase
    EC=6.3.2.6
    Alternative name(s):
    SAICAR synthetase
  2. Phosphoribosylaminoimidazole carboxylase
    EC=4.1.1.21
    Alternative name(s):
    AIR carboxylase
    Short name=AIRC
Gene names
Name:Paics
Synonyms:Ade2, Airc
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.

5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.

Subunit structure

Homooctamer By similarity.

Sequence similarities

In the N-terminal section; belongs to the SAICAR synthetase family.

In the C-terminal section; belongs to the AIR carboxylase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 425424Multifunctional protein ADE2
PRO_0000075033

Regions

Region2 – 260259SAICAR synthetase
Region261 – 425165AIR carboxylase
Region261 – 2666Linker By similarity
Region267 – 425159AIR carboxylase By similarity

Sites

Active site1011For SAICAR synthetase activity By similarity
Active site1071For SAICAR synthetase activity By similarity
Active site2151For SAICAR synthetase activity By similarity
Active site3031For AIR carboxylase activity By similarity
Active site3321For AIR carboxylase activity By similarity
Site3341Essential for AIR carboxylase activity By similarity

Amino acid modifications

Modified residue21N-acetylalanine By similarity
Modified residue221Phosphotyrosine By similarity
Modified residue271Phosphoserine By similarity
Modified residue531N6-acetyllysine By similarity
Modified residue1051Phosphothreonine By similarity
Modified residue1071Phosphoserine By similarity
Modified residue2381Phosphothreonine By similarity
Modified residue2471N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P51583 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 8E097CFD114E9DAB

FASTA42547,096
        10         20         30         40         50         60 
MATAEVLNIG RKLYEGKTKE VYELLDSPGR VLLQSKDQIT AGNAARKNHL EGKAAISNKI 

        70         80         90        100        110        120 
TSCIFQLLQE AGIKTAFTKK CGETAFIAPQ CEMIPIEWVC RRIATGSFLK RNPGVKEGYR 

       130        140        150        160        170        180 
FYPPKVEMFF KDDANNDPQW SEEQLIAAKF CFAGLVIGQT EVDIMSHATQ AIFEILEKSW 

       190        200        210        220        230        240 
LPQNCTLVDM KIEFGVDVTT KEIVLADVID NDSWRLWPSG DRSQQKDKQS YRDLKEVTPE 

       250        260        270        280        290        300 
GLQMVKKNFE WVADRVELLL KSNSQCRVVV LMGSTSDLGH CEKIKKACGN FGIPCELRVT 

       310        320        330        340        350        360 
SAHKGPDETL RIKAEYEGDG IPTVFVAVAG RSNGLGPVMS GNTAYPVISC PPITADWGAQ 

       370        380        390        400        410        420 
DVWSSLRLPS GIGCSTILSP EGSAQFAAQI FGLNNHLVWA KLRASKLNTW ISLKQADKKI 


RECNL

« Hide

References

« Hide 'large scale' references
[1]"Rat genomic structure of amidophosphoribosyltransferase, cDNA sequence of aminoimidazole ribonucleotide carboxylase, and cell cycle-dependent expression of these two physically linked genes."
Iwahana H., Honda S., Tsujisawa T., Takahashi Y., Adzuma K., Katashima R., Yamaoka T., Moritani M., Yoshimoto K., Itakura M.
Biochim. Biophys. Acta 1261:369-380(1995) [PubMed: 7742366] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Fischer.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D37979 mRNA. Translation: BAA07197.1.
BC072508 mRNA. Translation: AAH72508.1.
BC085711 mRNA. Translation: AAH85711.1.
IPIIPI00203391.
PIRS55684.
RefSeqNP_543186.1. NM_080910.3.
UniGeneRn.3015.

3D structure databases

ProteinModelPortalP51583.
SMRP51583. Positions 7-425.
ModBaseSearch...

Protein-protein interaction databases

IntActP51583. 1 interaction.
STRINGP51583.

Proteomic databases

PRIDEP51583.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000063942; ENSRNOP00000061495; ENSRNOG00000002101.
GeneID140946.
KEGGrno:140946.
UCSCNM_080910. rat.

Organism-specific databases

CTD10606.
RGD620066. Paics.

Phylogenomic databases

eggNOGroNOG04033.
GeneTreeENSGT00390000010172.
HOVERGENHBG008335.
InParanoidP51583.
OrthoDBEOG44BB27.
PhylomeDBP51583.

Gene expression databases

ArrayExpressP51583.
GenevestigatorP51583.
GermOnlineENSRNOG00000002101. Rattus norvegicus.

Family and domain databases

InterProIPR013816. ATP_grasp_subdomain_2.
IPR000031. N5-CAIR_Mutase_PurE_dom.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
Gene3DG3DSA:3.40.50.7700. AIR_carboxyl. 1 hit.
G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KOK01587.
PANTHERPTHR11609. SAICAR_synt. 1 hit.
PfamPF00731. AIRC. 1 hit.
PF01259. SAICAR_synt. 1 hit.
[Graphical view]
SMARTSM01001. AIRC. 1 hit.
[Graphical view]
SUPFAMSSF52255. AIR_carboxyl. 1 hit.
TIGRFAMsTIGR01162. PurE. 1 hit.
PROSITEPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio620860.

Entry information

Entry namePUR6_RAT
AccessionPrimary (citable) accession number: P51583
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: January 25, 2012
This is version 87 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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