Reviewed, UniProtKB/Swiss-Prot P51583 (PUR6_RAT)

Names and origin

Protein names

Recommended name:
    Multifunctional protein ADE2
Including the following 2 domains:
    1- Recommended name:
            Phosphoribosylaminoimidazole-succinocarboxamide synthase
              EC=6.3.2.6
        Alternative name(s):
            SAICAR synthetase
    2- Recommended name:
            Phosphoribosylaminoimidazole carboxylase
              EC=4.1.1.21
        Alternative name(s):
            AIR carboxylase
              Short name=AIRC

Gene names

Name:	Paics
Synonyms:	Ade2, Airc

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	425 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
Subunit structure	Homooctamer By similarity.
Sequence similarities	In the N-terminal section; belongs to the SAICAR synthetase family. In the C-terminal section; belongs to the AIR carboxylase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Decarboxylase Ligase Lyase
PTM	Acetylation Phosphoprotein
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazole carboxylase activity Inferred from electronic annotation. Source: EC phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 425	424	Multifunctional protein ADE2		PRO_0000075033
Regions
	Region	2 – 260	259	SAICAR synthetase
	Region	261 – 425	165	AIR carboxylase
	Region	261 – 266	6	Linker By similarity
	Region	267 – 425	159	AIR carboxylase By similarity
Sites
	Active site	101	1	For SAICAR synthetase activity By similarity
	Active site	107	1	For SAICAR synthetase activity By similarity
	Active site	215	1	For SAICAR synthetase activity By similarity
	Active site	303	1	For AIR carboxylase activity By similarity
	Active site	332	1	For AIR carboxylase activity By similarity
	Site	334	1	Essential for AIR carboxylase activity By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	22	1	Phosphotyrosine By similarity
	Modified residue	27	1	Phosphoserine By similarity
	Modified residue	53	1	N6-acetyllysine By similarity
	Modified residue	105	1	Phosphothreonine By similarity
	Modified residue	107	1	Phosphoserine By similarity
	Modified residue	238	1	Phosphothreonine By similarity
	Modified residue	247	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51583-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 8E097CFD114E9DAB	FASTA	425	47,096
	10 20 30 40 50 60 MATAEVLNIG RKLYEGKTKE VYELLDSPGR VLLQSKDQIT AGNAARKNHL EGKAAISNKI 70 80 90 100 110 120 TSCIFQLLQE AGIKTAFTKK CGETAFIAPQ CEMIPIEWVC RRIATGSFLK RNPGVKEGYR 130 140 150 160 170 180 FYPPKVEMFF KDDANNDPQW SEEQLIAAKF CFAGLVIGQT EVDIMSHATQ AIFEILEKSW 190 200 210 220 230 240 LPQNCTLVDM KIEFGVDVTT KEIVLADVID NDSWRLWPSG DRSQQKDKQS YRDLKEVTPE 250 260 270 280 290 300 GLQMVKKNFE WVADRVELLL KSNSQCRVVV LMGSTSDLGH CEKIKKACGN FGIPCELRVT 310 320 330 340 350 360 SAHKGPDETL RIKAEYEGDG IPTVFVAVAG RSNGLGPVMS GNTAYPVISC PPITADWGAQ 370 380 390 400 410 420 DVWSSLRLPS GIGCSTILSP EGSAQFAAQI FGLNNHLVWA KLRASKLNTW ISLKQADKKI RECNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Rat genomic structure of amidophosphoribosyltransferase, cDNA sequence of aminoimidazole ribonucleotide carboxylase, and cell cycle-dependent expression of these two physically linked genes." Iwahana H., Honda S., Tsujisawa T., Takahashi Y., Adzuma K., Katashima R., Yamaoka T., Moritani M., Yoshimoto K., Itakura M. Biochim. Biophys. Acta 1261:369-380(1995) [PubMed: 7742366] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Heart.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D37979 mRNA. Translation: BAA07197.1. BC072508 mRNA. Translation: AAH72508.1. BC085711 mRNA. Translation: AAH85711.1.
IPI	IPI00203391.
PIR	S55684.
RefSeq	NP_543186.1.
UniGene	Rn.3015
3D structure databases
SMR	P51583. Positions 7-425.
ModBase	Search...
Protein-protein interaction databases
STRING	P51583.
Proteomic databases
PRIDE	P51583.
Genome annotation databases
Ensembl	ENSRNOT00000063942; ENSRNOP00000061495; ENSRNOG00000002101; Rattus norvegicus. [Genome view]
GeneID	140946.
KEGG	rno:140946.
UCSC	NM_080910. rat.
Organism-specific databases
CTD	140946.
RGD	620066. Paics.
Phylogenomic databases
eggNOG	roNOG04033.
HOVERGEN	P51583.
InParanoid	P51583.
PhylomeDB	P51583.
Enzyme and pathway databases
BRENDA	4.1.1.21. 248. 6.3.2.6. 248.
Gene expression databases
ArrayExpress	P51583.
Genevestigator	P51583.
GermOnline	ENSRNOG00000002101. Rattus norvegicus.
Family and domain databases
InterPro	IPR000031. AIR_COase_core. IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synt. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.7700. AIR_carboxyl. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF00731. AIRC. 1 hit. PF01259. SAICAR_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR01162. purE. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	620860.

Entry information

Entry name

PUR6_RAT

Accession

Primary (citable) accession number: P51583

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)