Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

P2Y purinoceptor 4

Gene

P2RY4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for UTP and UDP coupled to G-proteins that activate a phosphatidylinositol-calcium second messenger system. Not activated by ATP or ADP.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-HSA-417957. P2Y receptors.
R-HSA-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
P2Y purinoceptor 4
Short name:
P2Y4
Alternative name(s):
P2P
Uridine nucleotide receptor
Short name:
UNR
Gene namesi
Name:P2RY4
Synonyms:NRU
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome X

Organism-specific databases

HGNCiHGNC:8542. P2RY4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 34ExtracellularSequence analysisAdd BLAST34
Transmembranei35 – 61Helical; Name=1Sequence analysisAdd BLAST27
Topological domaini62 – 72CytoplasmicSequence analysisAdd BLAST11
Transmembranei73 – 95Helical; Name=2Sequence analysisAdd BLAST23
Topological domaini96 – 112ExtracellularSequence analysisAdd BLAST17
Transmembranei113 – 131Helical; Name=3Sequence analysisAdd BLAST19
Topological domaini132 – 154CytoplasmicSequence analysisAdd BLAST23
Transmembranei155 – 174Helical; Name=4Sequence analysisAdd BLAST20
Topological domaini175 – 196ExtracellularSequence analysisAdd BLAST22
Transmembranei197 – 222Helical; Name=5Sequence analysisAdd BLAST26
Topological domaini223 – 246CytoplasmicSequence analysisAdd BLAST24
Transmembranei247 – 269Helical; Name=6Sequence analysisAdd BLAST23
Topological domaini270 – 287ExtracellularSequence analysisAdd BLAST18
Transmembranei288 – 309Helical; Name=7Sequence analysisAdd BLAST22
Topological domaini310 – 365CytoplasmicSequence analysisAdd BLAST56

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi243S → A: No effect. 1 Publication1
Mutagenesisi333 – 365Missing : Abolishes agonist-induced phosphorylation. Prevents agonist-induced desensitization and loss of cell surface receptors. Add BLAST33
Mutagenesisi333 – 359SSLAL…SSCST → AALALVALPEDAACRWAAAP QDAACAA: Greatly reduces agonist-induced desensitization and loss of cell surface receptors. Add BLAST27
Mutagenesisi333S → A: Greatly reduces agonist-induced desensitization and loss of cell surface receptors; when associated with A-334 and A-339. 1 Publication1
Mutagenesisi334S → A: Greatly reduces agonist-induced desensitization and loss of cell surface receptors; when associated with A-333 and A-339. 1 Publication1
Mutagenesisi339S → A: Greatly reduces agonist-induced desensitization and loss of cell surface receptors; when associated with A-333 and A-334. 1 Publication1
Mutagenesisi344 – 365Missing : No effect on agonist-induced phosphorylation, no functional effect. Add BLAST22
Mutagenesisi356 – 365Missing : No functional effect. 10

Organism-specific databases

DisGeNETi5030.
OpenTargetsiENSG00000186912.
PharmGKBiPA32871.

Chemistry databases

ChEMBLiCHEMBL2123.
GuidetoPHARMACOLOGYi325.

Polymorphism and mutation databases

BioMutaiP2RY4.
DMDMi1709524.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000700201 – 365P2Y purinoceptor 4Add BLAST365

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi108 ↔ 185PROSITE-ProRule annotation
Modified residuei333Phosphoserine1 Publication1
Modified residuei334Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation of Ser-333 and Ser-334 is a key step in agonist-dependent desensitization and loss of surface P2RY4. This phosphorylation does not involve PKC, nor other calcium activated kinases.1 Publication

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP51582.
PeptideAtlasiP51582.
PRIDEiP51582.

PTM databases

iPTMnetiP51582.
PhosphoSitePlusiP51582.

Expressioni

Tissue specificityi

Pancreas.

Gene expression databases

BgeeiENSG00000186912.
CleanExiHS_P2RY4.
GenevisibleiP51582. HS.

Organism-specific databases

HPAiCAB022644.

Interactioni

Protein-protein interaction databases

IntActiP51582. 1 interactor.
STRINGi9606.ENSP00000363643.

Chemistry databases

BindingDBiP51582.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B6Qmodel-A1-365[»]
ProteinModelPortaliP51582.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIAQ. Eukaryota.
ENOG410XQNZ. LUCA.
GeneTreeiENSGT00760000118784.
HOGENOMiHOG000231307.
HOVERGENiHBG101120.
InParanoidiP51582.
KOiK04271.
OMAiDATATYM.
OrthoDBiEOG091G09D4.
PhylomeDBiP51582.
TreeFamiTF350009.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000018. P2Y4.
[Graphical view]
PANTHERiPTHR24231:SF21. PTHR24231:SF21. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01066. P2Y4PRNOCPTR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51582-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTESSLLR SLGLSPGPGS SEVELDCWFD EDFKFILLPV SYAVVFVLGL
60 70 80 90 100
GLNAPTLWLF IFRLRPWDAT ATYMFHLALS DTLYVLSLPT LIYYYAAHNH
110 120 130 140 150
WPFGTEICKF VRFLFYWNLY CSVLFLTCIS VHRYLGICHP LRALRWGRPR
160 170 180 190 200
LAGLLCLAVW LVVAGCLVPN LFFVTTSNKG TTVLCHDTTR PEEFDHYVHF
210 220 230 240 250
SSAVMGLLFG VPCLVTLVCY GLMARRLYQP LPGSAQSSSR LRSLRTIAVV
260 270 280 290 300
LTVFAVCFVP FHITRTIYYL ARLLEADCRV LNIVNVVYKV TRPLASANSC
310 320 330 340 350
LDPVLYLLTG DKYRRQLRQL CGGGKPQPRT AASSLALVSL PEDSSCRWAA
360
TPQDSSCSTP RADRL
Length:365
Mass (Da):40,963
Last modified:October 1, 1996 - v1
Checksum:i23E0AFED3B7BDEED
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti86L → V in AAC50347 (PubMed:8537335).Curated1
Sequence conflicti121C → S in AAH96068 (PubMed:15489334).Curated1
Sequence conflicti247I → V in AAH96069 (PubMed:15489334).Curated1
Sequence conflicti352P → T in AAH96069 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_011854168V → M.Corresponds to variant rs1152186dbSNPEnsembl.1
Natural variantiVAR_011855178N → T.2 PublicationsCorresponds to variant rs1152187dbSNPEnsembl.1
Natural variantiVAR_011856191P → L.Corresponds to variant rs1152188dbSNPEnsembl.1
Natural variantiVAR_049429234S → A.1 PublicationCorresponds to variant rs3829709dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91852 Genomic DNA. Translation: CAA62963.1.
U40223 Genomic DNA. Translation: AAC50347.1.
X96597 Genomic DNA. Translation: CAA65415.1.
AL357752 Genomic DNA. Translation: CAI41492.1.
BC095503 mRNA. Translation: AAH95503.1.
BC096067 mRNA. Translation: AAH96067.1.
BC096068 mRNA. Translation: AAH96068.1.
BC096069 mRNA. Translation: AAH96069.1.
BC096070 mRNA. Translation: AAH96070.1.
CCDSiCCDS14398.1.
PIRiS68679.
RefSeqiNP_002556.1. NM_002565.3.
UniGeneiHs.673854.

Genome annotation databases

EnsembliENST00000374519; ENSP00000363643; ENSG00000186912.
GeneIDi5030.
KEGGihsa:5030.
UCSCiuc004dxz.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X91852 Genomic DNA. Translation: CAA62963.1.
U40223 Genomic DNA. Translation: AAC50347.1.
X96597 Genomic DNA. Translation: CAA65415.1.
AL357752 Genomic DNA. Translation: CAI41492.1.
BC095503 mRNA. Translation: AAH95503.1.
BC096067 mRNA. Translation: AAH96067.1.
BC096068 mRNA. Translation: AAH96068.1.
BC096069 mRNA. Translation: AAH96069.1.
BC096070 mRNA. Translation: AAH96070.1.
CCDSiCCDS14398.1.
PIRiS68679.
RefSeqiNP_002556.1. NM_002565.3.
UniGeneiHs.673854.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2B6Qmodel-A1-365[»]
ProteinModelPortaliP51582.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP51582. 1 interactor.
STRINGi9606.ENSP00000363643.

Chemistry databases

BindingDBiP51582.
ChEMBLiCHEMBL2123.
GuidetoPHARMACOLOGYi325.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiP51582.
PhosphoSitePlusiP51582.

Polymorphism and mutation databases

BioMutaiP2RY4.
DMDMi1709524.

Proteomic databases

PaxDbiP51582.
PeptideAtlasiP51582.
PRIDEiP51582.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000374519; ENSP00000363643; ENSG00000186912.
GeneIDi5030.
KEGGihsa:5030.
UCSCiuc004dxz.2. human.

Organism-specific databases

CTDi5030.
DisGeNETi5030.
GeneCardsiP2RY4.
HGNCiHGNC:8542. P2RY4.
HPAiCAB022644.
MIMi300038. gene.
neXtProtiNX_P51582.
OpenTargetsiENSG00000186912.
PharmGKBiPA32871.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IIAQ. Eukaryota.
ENOG410XQNZ. LUCA.
GeneTreeiENSGT00760000118784.
HOGENOMiHOG000231307.
HOVERGENiHBG101120.
InParanoidiP51582.
KOiK04271.
OMAiDATATYM.
OrthoDBiEOG091G09D4.
PhylomeDBiP51582.
TreeFamiTF350009.

Enzyme and pathway databases

ReactomeiR-HSA-417957. P2Y receptors.
R-HSA-418594. G alpha (i) signalling events.

Miscellaneous databases

GeneWikiiP2RY4.
GenomeRNAii5030.
PROiP51582.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000186912.
CleanExiHS_P2RY4.
GenevisibleiP51582. HS.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR000018. P2Y4.
[Graphical view]
PANTHERiPTHR24231:SF21. PTHR24231:SF21. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR01066. P2Y4PRNOCPTR.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2RY4_HUMAN
AccessioniPrimary (citable) accession number: P51582
Secondary accession number(s): Q4VBB7
, Q4VBB8, Q502W2, Q5JT22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. Human chromosome X
    Human chromosome X: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.