P51577 (P2RX4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2X purinoceptor 4
Short name=P2X4

Alternative name(s):
ATP receptor
Purinergic receptor

Gene names

Name:

P2rx4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
Subunit structure	Functional P2XRs are organized as homomeric and heteromeric trimers By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Widespread distribution in the brain. Also expressed in epithelial cells.
Sequence similarities	Belongs to the P2X receptor family.

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ion channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	calcium ion transport Inferred from electronic annotation. Source: Compara negative regulation of cardiac muscle hypertrophy Inferred from electronic annotation. Source: Compara nitric oxide biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of calcium ion transport into cytosol Inferred from electronic annotation. Source: Compara positive regulation of calcium-mediated signaling Inferred from electronic annotation. Source: Compara protein homooligomerization Inferred from direct assay PubMed 15313628. Source: RGD regulation of action potential in neuron Inferred from direct assay PubMed 12819199PubMed 16282518PubMed 16934235. Source: RGD regulation of apoptotic process Inferred from electronic annotation. Source: Compara regulation of blood pressure Inferred from electronic annotation. Source: Compara regulation of cardiac muscle contraction Inferred from electronic annotation. Source: Compara regulation of excitatory postsynaptic membrane potential Inferred from electronic annotation. Source: Compara regulation of sodium ion transport Inferred from direct assay PubMed 17785580. Source: BHF-UCL relaxation of cardiac muscle Inferred from electronic annotation. Source: Compara response to ATP Inferred from electronic annotation. Source: Compara response to fluid shear stress Inferred from electronic annotation. Source: Compara sensory perception of pain Inferred from mutant phenotype PubMed 14978347. Source: BHF-UCL vasodilation Inferred from electronic annotation. Source: Compara
Cellular_component	apical part of cell Inferred from electronic annotation. Source: Compara cell junction Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay PubMed 11160443PubMed 15476696. Source: RGD perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay PubMed 17785580. Source: BHF-UCL postsynaptic density Inferred from direct assay PubMed 11160443PubMed 15476696. Source: RGD terminal bouton Inferred from direct assay PubMed 15476696. Source: RGD
Molecular_function	ATP binding Inferred by curator PubMed 12819199. Source: BHF-UCL copper ion binding Inferred from direct assay PubMed 12819199. Source: BHF-UCL drug binding Inferred from direct assay PubMed 16282518. Source: RGD extracellular ATP-gated cation channel activity Inferred from direct assay PubMed 12819199. Source: BHF-UCL purinergic nucleotide receptor activity Inferred from direct assay PubMed 12819199. Source: BHF-UCL zinc ion binding Inferred from direct assay PubMed 12819199. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 388

388

P2X purinoceptor 4

PRO_0000161554

Regions

Topological domain

1 – 33

33

Cytoplasmic Potential

Transmembrane

34 – 54

21

Helical; Name=1; Potential

Topological domain

55 – 338

284

Extracellular Potential

Transmembrane

339 – 359

21

Helical; Name=2; Potential

Topological domain

360 – 388

29

Cytoplasmic Potential

Amino acid modifications

Modified residue

378

1

Phosphotyrosine By similarity

Glycosylation

75

1

N-linked (GlcNAc...) Potential

Glycosylation

110

1

N-linked (GlcNAc...) Potential

Glycosylation

153

1

N-linked (GlcNAc...) Potential

Glycosylation

184

1

N-linked (GlcNAc...) Potential

Glycosylation

199

1

N-linked (GlcNAc...) Potential

Glycosylation

208

1

N-linked (GlcNAc...) Potential

Disulfide bond

116 ↔ 165

By similarity

Disulfide bond

126 ↔ 149

By similarity

Disulfide bond

132 ↔ 159

By similarity

Disulfide bond

217 ↔ 227

By similarity

Disulfide bond

261 ↔ 270

By similarity

Experimental info

Mutagenesis

249

1

E → K: Restores antagonism by PPADS. Ref.4

Sequence conflict

136 – 137

2

SV → LR in AAC52380. Ref.1

Sequence conflict

237

1

E → G in CAA61037. Ref.4

Sequence conflict

305

1

G → A in CAA62607. Ref.2

Secondary structure

1

.

.

.

388

Helix Strand Turn

Details...

Beta strand

379 – 381

3

Sequences

	Sequence		Length	Mass (Da)
	P51577 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C7B0FC9A94EF8D9A	FASTA	388	43,501
	10 20 30 40 50 60 MAGCCSVLGS FLFEYDTPRI VLIRSRKVGL MNRAVQLLIL AYVIGWVFVW EKGYQETDSV 70 80 90 100 110 120 VSSVTTKAKG VAVTNTSQLG FRIWDVADYV IPAQEENSLF IMTNMIVTVN QTQSTCPEIP 130 140 150 160 170 180 DKTSICNSDA DCTPGSVDTH SSGVATGRCV PFNESVKTCE VAAWCPVEND VGVPTPAFLK 190 200 210 220 230 240 AAENFTLLVK NNIWYPKFNF SKRNILPNIT TSYLKSCIYN AQTDPFCPIF RLGTIVEDAG 250 260 270 280 290 300 HSFQEMAVEG GIMGIQIKWD CNLDRAASLC LPRYSFRRLD TRDLEHNVSP GYNFRFAKYY 310 320 330 340 350 360 RDLAGKEQRT LTKAYGIRFD IIVFGKAGKF DIIPTMINVG SGLALLGVAT VLCDVIVLYC 370 380 MKKKYYYRDK KYKYVEDYEQ GLSGEMNQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel neuronal P2x ATP receptor ion channel with widespread distribution in the brain." Seguela P., Haghighi A., Soghomonian J.J., Cooper E. J. Neurosci. 16:448-455(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Forebrain.
[2]	"A P2X purinoceptor cDNA conferring a novel pharmacological profile." Bo X., Zhang Y., Nassar M., Burnstock G., Schoepfer R. FEBS Lett. 375:129-133(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning and pharmacological characterization of a fourth P2X receptor subtype widely expressed in brain and peripheral tissues including various endocrine tissues." Wang C.-Z., Namba N., Gonoi T., Inagaki N., Seino S. Biochem. Biophys. Res. Commun. 220:196-202(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreatic islet.
[4]	"An antagonist-insensitive P2X receptor expressed in epithelia and brain." Buell G.N., Lewis C., Collo G., North R.A., Surprenant A. EMBO J. 15:55-62(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF GLU-249. Tissue: Superior cervical ganglion.
[5]	"P2X4: an ATP-activated ionotropic receptor cloned from rat brain." Soto F., Garcia-Guzman M., Gomez-Hernandez J.M., Hollmann M., Karschin C., Stuemer W. Proc. Natl. Acad. Sci. U.S.A. 93:3684-3688(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U32497 mRNA. Translation: AAC52380.1.
X91200 mRNA. Translation: CAA62607.1.
U47031 mRNA. Translation: AAA99777.1.
X87763 mRNA. Translation: CAA61037.1.
X93565 mRNA. Translation: CAA63778.1.
BC078792 mRNA. Translation: AAH78792.1.

IPI

IPI00324987.

PIR

JC6137.

RefSeq

NP_113782.1. NM_031594.1.

UniGene

Rn.7176.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BP5	X-ray	2.80	P	375-384	[»]

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-48411N.

STRING

10116.ENSRNOP00000001752.

Proteomic databases

PaxDb

P51577.

PRIDE

P51577.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000001752; ENSRNOP00000001752; ENSRNOG00000001300.

GeneID

29659.

KEGG

rno:29659.

UCSC

RGD:62073. rat.

Organism-specific databases

CTD

5025.

RGD

62073. P2rx4.

Phylogenomic databases

eggNOG

NOG47843.

GeneTree

ENSGT00390000016028.

HOGENOM

HOG000232042.

HOVERGEN

HBG053086.

InParanoid

P51577.

KO

K05218.

OMA

TMINVGS.

OrthoDB

EOG418BNK.

Gene expression databases

ArrayExpress

P51577.

Genevestigator

P51577.

GermOnline

ENSRNOG00000001300. Rattus norvegicus.

Family and domain databases

Gene3D

2.60.490.10. 1 hit.

InterPro

IPR003047. P2X4_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]

PANTHER

PTHR10125. PTHR10125. 1 hit.
PTHR10125:SF18. PTHR10125:SF18. 1 hit.

Pfam

PF00864. P2X_receptor. 1 hit.
[Graphical view]

PRINTS

PR01311. P2X4RECEPTOR.
PR01307. P2XRECEPTOR.

TIGRFAMs

TIGR00863. P2X. 1 hit.

PROSITE

PS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

P51577.

ChEMBL

CHEMBL2818.

EvolutionaryTrace

P51577.

NextBio

609955.

Entry information

Entry name

P2RX4_RAT

Accession

Primary (citable) accession number: P51577

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program