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Protein

P2X purinoceptor 4

Gene

P2rx4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.

GO - Molecular functioni

  • ATP binding Source: BHF-UCL
  • copper ion binding Source: BHF-UCL
  • drug binding Source: RGD
  • extracellular ATP-gated cation channel activity Source: BHF-UCL
  • purinergic nucleotide receptor activity Source: BHF-UCL
  • receptor binding Source: BHF-UCL
  • zinc ion binding Source: BHF-UCL

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-139853. Elevation of cytosolic Ca2+ levels.
R-RNO-418346. Platelet homeostasis.

Names & Taxonomyi

Protein namesi
Recommended name:
P2X purinoceptor 4
Short name:
P2X4
Alternative name(s):
ATP receptor
Purinergic receptor
Gene namesi
Name:P2rx4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi62073. P2rx4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3333CytoplasmicSequence analysisAdd
BLAST
Transmembranei34 – 5421Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini55 – 338284ExtracellularSequence analysisAdd
BLAST
Transmembranei339 – 35921Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini360 – 38829CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • axon Source: RGD
  • cell junction Source: Ensembl
  • dendrite Source: RGD
  • dendritic spine Source: RGD
  • extracellular exosome Source: Ensembl
  • integral component of nuclear inner membrane Source: GO_Central
  • integral component of plasma membrane Source: UniProtKB
  • lysosomal membrane Source: Ensembl
  • neuronal cell body Source: RGD
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: BHF-UCL
  • postsynaptic density Source: RGD
  • synapse Source: RGD
  • terminal bouton Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi249 – 2491E → K: Restores antagonism by PPADS. 1 Publication

Chemistry

ChEMBLiCHEMBL2818.
GuidetoPHARMACOLOGYi481.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 388388P2X purinoceptor 4PRO_0000161554Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi75 – 751N-linked (GlcNAc...)Sequence analysis
Glycosylationi110 – 1101N-linked (GlcNAc...)Sequence analysis
Disulfide bondi116 ↔ 165By similarity
Disulfide bondi126 ↔ 149By similarity
Disulfide bondi132 ↔ 159By similarity
Glycosylationi153 – 1531N-linked (GlcNAc...)Sequence analysis
Glycosylationi184 – 1841N-linked (GlcNAc...)Sequence analysis
Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence analysis
Glycosylationi208 – 2081N-linked (GlcNAc...)Sequence analysis
Disulfide bondi217 ↔ 227By similarity
Disulfide bondi261 ↔ 270By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP51577.
PRIDEiP51577.

PTM databases

PhosphoSiteiP51577.

Expressioni

Tissue specificityi

Widespread distribution in the brain. Also expressed in epithelial cells.

Gene expression databases

BgeeiENSRNOG00000001300.
GenevisibleiP51577. RN.

Interactioni

Subunit structurei

Functional P2XRs are organized as homomeric and heteromeric trimers.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
P2rx2P496534EBI-9511617,EBI-9511543

GO - Molecular functioni

  • receptor binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi248281. 2 interactions.
DIPiDIP-48411N.
IntActiP51577. 2 interactions.
STRINGi10116.ENSRNOP00000001752.

Chemistry

BindingDBiP51577.

Structurei

Secondary structure

1
388
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi114 – 1174Combined sources
Turni129 – 1313Combined sources
Turni139 – 1413Combined sources
Beta strandi143 – 1508Combined sources
Beta strandi153 – 1553Combined sources
Beta strandi158 – 1636Combined sources
Beta strandi379 – 3813Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BP5X-ray2.80P375-384[»]
2RUPNMR-A111-167[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51577.

Family & Domainsi

Sequence similaritiesi

Belongs to the P2X receptor family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IFJF. Eukaryota.
ENOG410XR0C. LUCA.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiP51577.
KOiK05218.
OMAiTMINVGS.
OrthoDBiEOG091G0A2U.
PhylomeDBiP51577.
TreeFamiTF328633.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003047. P2X4_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF18. PTHR10125:SF18. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01311. P2X4RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51577-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGCCSVLGS FLFEYDTPRI VLIRSRKVGL MNRAVQLLIL AYVIGWVFVW
60 70 80 90 100
EKGYQETDSV VSSVTTKAKG VAVTNTSQLG FRIWDVADYV IPAQEENSLF
110 120 130 140 150
IMTNMIVTVN QTQSTCPEIP DKTSICNSDA DCTPGSVDTH SSGVATGRCV
160 170 180 190 200
PFNESVKTCE VAAWCPVEND VGVPTPAFLK AAENFTLLVK NNIWYPKFNF
210 220 230 240 250
SKRNILPNIT TSYLKSCIYN AQTDPFCPIF RLGTIVEDAG HSFQEMAVEG
260 270 280 290 300
GIMGIQIKWD CNLDRAASLC LPRYSFRRLD TRDLEHNVSP GYNFRFAKYY
310 320 330 340 350
RDLAGKEQRT LTKAYGIRFD IIVFGKAGKF DIIPTMINVG SGLALLGVAT
360 370 380
VLCDVIVLYC MKKKYYYRDK KYKYVEDYEQ GLSGEMNQ
Length:388
Mass (Da):43,501
Last modified:October 1, 1996 - v1
Checksum:iC7B0FC9A94EF8D9A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti136 – 1372SV → LR in AAC52380 (PubMed:8551329).Curated
Sequence conflicti237 – 2371E → G in CAA61037 (PubMed:8598206).Curated
Sequence conflicti305 – 3051G → A in CAA62607 (PubMed:7498461).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32497 mRNA. Translation: AAC52380.1.
X91200 mRNA. Translation: CAA62607.1.
U47031 mRNA. Translation: AAA99777.1.
X87763 mRNA. Translation: CAA61037.1.
X93565 mRNA. Translation: CAA63778.1.
BC078792 mRNA. Translation: AAH78792.1.
PIRiJC6137.
RefSeqiNP_113782.1. NM_031594.1.
UniGeneiRn.7176.

Genome annotation databases

EnsembliENSRNOT00000001752; ENSRNOP00000001752; ENSRNOG00000001300.
GeneIDi29659.
KEGGirno:29659.
UCSCiRGD:62073. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32497 mRNA. Translation: AAC52380.1.
X91200 mRNA. Translation: CAA62607.1.
U47031 mRNA. Translation: AAA99777.1.
X87763 mRNA. Translation: CAA61037.1.
X93565 mRNA. Translation: CAA63778.1.
BC078792 mRNA. Translation: AAH78792.1.
PIRiJC6137.
RefSeqiNP_113782.1. NM_031594.1.
UniGeneiRn.7176.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BP5X-ray2.80P375-384[»]
2RUPNMR-A111-167[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248281. 2 interactions.
DIPiDIP-48411N.
IntActiP51577. 2 interactions.
STRINGi10116.ENSRNOP00000001752.

Chemistry

BindingDBiP51577.
ChEMBLiCHEMBL2818.
GuidetoPHARMACOLOGYi481.

PTM databases

PhosphoSiteiP51577.

Proteomic databases

PaxDbiP51577.
PRIDEiP51577.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001752; ENSRNOP00000001752; ENSRNOG00000001300.
GeneIDi29659.
KEGGirno:29659.
UCSCiRGD:62073. rat.

Organism-specific databases

CTDi5025.
RGDi62073. P2rx4.

Phylogenomic databases

eggNOGiENOG410IFJF. Eukaryota.
ENOG410XR0C. LUCA.
GeneTreeiENSGT00390000016028.
HOGENOMiHOG000232042.
HOVERGENiHBG053086.
InParanoidiP51577.
KOiK05218.
OMAiTMINVGS.
OrthoDBiEOG091G0A2U.
PhylomeDBiP51577.
TreeFamiTF328633.

Enzyme and pathway databases

ReactomeiR-RNO-139853. Elevation of cytosolic Ca2+ levels.
R-RNO-418346. Platelet homeostasis.

Miscellaneous databases

EvolutionaryTraceiP51577.
PROiP51577.

Gene expression databases

BgeeiENSRNOG00000001300.
GenevisibleiP51577. RN.

Family and domain databases

Gene3Di2.60.490.10. 1 hit.
InterProiIPR003047. P2X4_purnocptor.
IPR027309. P2X_extracellular_dom.
IPR001429. P2X_purnocptor.
[Graphical view]
PANTHERiPTHR10125. PTHR10125. 1 hit.
PTHR10125:SF18. PTHR10125:SF18. 1 hit.
PfamiPF00864. P2X_receptor. 1 hit.
[Graphical view]
PIRSFiPIRSF005713. P2X_purinoceptor. 1 hit.
PRINTSiPR01311. P2X4RECEPTOR.
PR01307. P2XRECEPTOR.
TIGRFAMsiTIGR00863. P2X. 1 hit.
PROSITEiPS01212. P2X_RECEPTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiP2RX4_RAT
AccessioniPrimary (citable) accession number: P51577
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.