P51576 (P2RX1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
P2X purinoceptor 1
Short name=P2X1

Alternative name(s):
ATP receptor
Purinergic receptor

Gene names

Name:

P2rx1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Ligand-gated ion channel with relatively high calcium permeability. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Seems to be linked to apoptosis, by increasing the intracellular concentration of calcium in the presence of ATP, leading to programmed cell death By similarity.
Subunit structure	Homo- or heteropolymers By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the P2X receptor family.

Ontologies

Keywords
Biological process	Apoptosis Ion transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Ionic channel Ligand-gated ion channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of cysteine-type endopeptidase activity involved in apoptotic process Inferred from direct assay. Source: MGI ceramide biosynthetic process Inferred from direct assay. Source: MGI insemination Inferred from mutant phenotype. Source: MGI platelet activation Inferred from mutant phenotype. Source: MGI positive regulation of ion transport Inferred from mutant phenotype. Source: MGI regulation of calcium ion transport Inferred from mutant phenotype. Source: MGI regulation of vascular smooth muscle contraction Inferred from mutant phenotype. Source: MGI response to ATP Inferred from mutant phenotype. Source: MGI serotonin secretion by platelet Inferred from mutant phenotype. Source: MGI
Cellular component	external side of cell outer membrane Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW postsynaptic membrane Inferred from direct assay. Source: MGI
Molecular function	extracellular ATP-gated cation channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome purinergic nucleotide receptor activity Inferred from mutant phenotype. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	P2X purinoceptor 1		PRO_0000161546
Regions
	Topological domain	1 – 28	28	Cytoplasmic Potential
	Transmembrane	29 – 50	22	Helical; Name=1; Potential
	Topological domain	51 – 338	288	Extracellular Potential
	Transmembrane	339 – 358	20	Helical; Name=2; Potential
	Topological domain	359 – 399	41	Cytoplasmic Potential
	Region	331 – 338	8	Pore-forming motif Potential
	Compositional bias	351 – 354	4	Poly-Leu
Amino acid modifications
	Glycosylation	153	1	N-linked (GlcNAc...) Potential
	Glycosylation	184	1	N-linked (GlcNAc...) Potential
	Glycosylation	210	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Disulfide bond	117 ↔ 165		By similarity
	Disulfide bond	126 ↔ 149		By similarity
	Disulfide bond	132 ↔ 159		By similarity
	Disulfide bond	217 ↔ 227		By similarity
	Disulfide bond	261 ↔ 270		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51576 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8CBF6B97F569472E	FASTA	399	44,851
	10 20 30 40 50 60 MARRLQDELS AFFFEYDTPR MVLVRNKKVG VIFRLIQLVV LVYVIGWVFV YEKGYQTSSG 70 80 90 100 110 120 LISSVSVKLK GLAVTQLQGL GPQVWDVADY VFPAHGDSSF VVMTNFIMTP QQAQGHCAEN 130 140 150 160 170 180 PEGGICQDDS GCTPGKAERK AQGIRTGNCV PFNGTVKTCE IFGWCPVEVD DKIPSPALLH 190 200 210 220 230 240 EAENFTLFIK NSISFPRFKV NRRNLVEEVN GTYMKKCLYH KILHPLCPVF SLGYVVRESG 250 260 270 280 290 300 QDFRSLAEKG GVVGITIDWE CDLDWHVRHC KPIYQFHGLY GEKNLSPGFN FRFARHFVQN 310 320 330 340 350 360 GTNRRHLFKV FGIRFDILVD GKAGKFDIIP TMTTIGSGIG IFGVATVLCD LLLLHILPKR 370 380 390 HYYKQKKFKY AEDMGPGEGE RDPAATSSTL GLQENMRTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization and chromosomal localization of a human P2X receptor from the urinary bladder." Valera S., Talabot F., Evans R.J., Gos A., Antonarakis S.E., Morris M.A., Buell G.N. Recept. Channels 3:283-289(1995) [PubMed: 8834001] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: OF1. Tissue: Urinary bladder smooth muscle.
[2]	"Mouse P2X1 purinergic receptor gene structure." Yu X.-M., Connolly A.J. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/SvJ.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Urinary bladder.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X84896 mRNA. Translation: CAA59322.1. AF250123, AF250121, AF250122 Genomic DNA. Translation: AAF68968.1. AK035304 mRNA. Translation: BAC29024.1. AL670399 Genomic DNA. Translation: CAI24783.1.
IPI	IPI00123864.
RefSeq	NP_032797.3. NM_008771.3.
UniGene	Mm.25722.
3D structure databases
ProteinModelPortal	P51576.
SMR	P51576. Positions 30-354.
ModBase	Search...
Protein-protein interaction databases
STRING	P51576.
PTM databases
PhosphoSite	P51576.
Proteomic databases
PRIDE	P51576.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000021141; ENSMUSP00000021141; ENSMUSG00000020787.
GeneID	18436.
KEGG	mmu:18436.
Organism-specific databases
CTD	5023.
MGI	MGI:1098235. P2rx1.
Phylogenomic databases
eggNOG	maNOG05754.
HOGENOM	HBG713324.
HOVERGEN	HBG053086.
InParanoid	P51576.
OMA	FNFRFAR.
OrthoDB	EOG42FSHT.
PhylomeDB	P51576.
Gene expression databases
ArrayExpress	P51576.
Bgee	P51576.
Genevestigator	P51576.
GermOnline	ENSMUSG00000020787. Mus musculus.
Family and domain databases
InterPro	IPR003044. P2X1_purnocptor. IPR001429. P2X_purnocptor. [Graphical view]
KO	K05215.
PANTHER	PTHR10125. ATP_P2X_rcpt. 1 hit. PTHR10125:SF9. PTHR10125:SF9. 1 hit.
Pfam	PF00864. P2X_receptor. 1 hit. [Graphical view]
PRINTS	PR01308. P2X1RECEPTOR. PR01307. P2XRECEPTOR.
TIGRFAMs	TIGR00863. P2X. 1 hit.
PROSITE	PS01212. P2X_RECEPTOR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	294106.
SOURCE	Search...

Entry information

Entry name

P2RX1_MOUSE

Accession

Primary (citable) accession number: P51576
Secondary accession number(s): Q5SRU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program