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Protein

Solute carrier family 15 member 1

Gene

Slc15a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Proton-coupled intake of oligopeptides of 2 to 4 amino acids with a preference for dipeptides. May constitute a major route for the absorption of protein digestion end-products.

GO - Molecular functioni

  • channel inhibitor activity Source: RGD
  • oligopeptide transporter activity Source: InterPro
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • negative regulation of amino acid transport Source: RGD
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Peptide transport, Protein transport, Symport, Transport

Protein family/group databases

TCDBi2.A.17.4.1. the proton-dependent oligopeptide transporter (pot/ptr) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 15 member 1
Alternative name(s):
Intestinal H(+)/peptide cotransporter
Oligopeptide transporter, small intestine isoform
Peptide transporter 1
Proton-coupled dipeptide cotransporter
Gene namesi
Name:Slc15a1
Synonyms:Pept1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621736. Slc15a1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalSequence analysisAdd
BLAST
Topological domaini22 – 5332ExtracellularSequence analysisAdd
BLAST
Transmembranei54 – 7421HelicalSequence analysisAdd
BLAST
Topological domaini75 – 828CytoplasmicSequence analysis
Transmembranei83 – 10321HelicalSequence analysisAdd
BLAST
Topological domaini104 – 11815ExtracellularSequence analysisAdd
BLAST
Transmembranei119 – 13921HelicalSequence analysisAdd
BLAST
Topological domaini140 – 16122CytoplasmicSequence analysisAdd
BLAST
Transmembranei162 – 18221HelicalSequence analysisAdd
BLAST
Topological domaini183 – 19816ExtracellularSequence analysisAdd
BLAST
Transmembranei199 – 21921HelicalSequence analysisAdd
BLAST
Topological domaini220 – 27657CytoplasmicSequence analysisAdd
BLAST
Transmembranei277 – 29721HelicalSequence analysisAdd
BLAST
Topological domaini298 – 32730ExtracellularSequence analysisAdd
BLAST
Transmembranei328 – 34821HelicalSequence analysisAdd
BLAST
Topological domaini349 – 36113CytoplasmicSequence analysisAdd
BLAST
Transmembranei362 – 38221HelicalSequence analysisAdd
BLAST
Topological domaini383 – 586204ExtracellularSequence analysisAdd
BLAST
Transmembranei587 – 60721HelicalSequence analysisAdd
BLAST
Topological domaini608 – 62114CytoplasmicSequence analysisAdd
BLAST
Transmembranei622 – 64221HelicalSequence analysisAdd
BLAST
Topological domaini643 – 6475ExtracellularSequence analysis
Transmembranei648 – 66821HelicalSequence analysisAdd
BLAST
Topological domaini669 – 71042CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL2073691.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 710710Solute carrier family 15 member 1PRO_0000064307Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi415 – 4151N-linked (GlcNAc...)Sequence analysis
Glycosylationi439 – 4391N-linked (GlcNAc...)Sequence analysis
Glycosylationi510 – 5101N-linked (GlcNAc...)Sequence analysis
Glycosylationi532 – 5321N-linked (GlcNAc...)Sequence analysis
Glycosylationi539 – 5391N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP51574.
PRIDEiP51574.

Expressioni

Tissue specificityi

Isoform 1 is highly expressed in small intestine. Isoform 2 is restricted to pinealocytes.4 Publications

Inductioni

Isoform 2 exhibits night/day variations with close to 100-fold increased expression at night. Up-regulation is due to a large degree to the release of norepinephrine from nerve terminals in the pineal gland and cAMP signaling pathway, which activates an alternative promoter within intron 20 resulting in isoform 2 production. Levels rapidly decrease after exposure to daylight and become undetectable at midday and late afternoon.2 Publications

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015890.

Chemistry

BindingDBiP51574.

Structurei

3D structure databases

ProteinModelPortaliP51574.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1237. Eukaryota.
COG3104. LUCA.
HOGENOMiHOG000285186.
HOVERGENiHBG052962.
InParanoidiP51574.
KOiK14206.
PhylomeDBiP51574.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR004768. Oligopep_transport.
IPR000109. POT_fam.
IPR018456. PTR2_symporter_CS.
[Graphical view]
PANTHERiPTHR11654. PTHR11654. 2 hits.
PfamiPF00854. PTR2. 2 hits.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00926. 2A1704. 1 hit.
PROSITEiPS01022. PTR2_1. 1 hit.
PS01023. PTR2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

Isoform 1 (identifier: P51574-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGMSKSRGCF GYPLSIFFIV VNEFCERFSY YGMRALLVLY FRNFLGWDDD
60 70 80 90 100
LSTAIYHTFV ALCYLTPILG ALIADSWLGK FKTIVSLSIV YTIGQAVISV
110 120 130 140 150
SSINDLTDHD HDGSPNNLPL HVALSMIGLA LIALGTGGIK PCVSAFGGDQ
160 170 180 190 200
FEEGQEKQRN RFFSIFYLAI NAGSLLSTII TPILRVQQCG IHSQQACYPL
210 220 230 240 250
AFGVPAALMA VALIVFVLGS GMYKKFQPQG NIMGKVAKCI RFAIKNRFRH
260 270 280 290 300
RSKAFPKRNH WLDWAKEKYD ERLISQIKIM TKVMFLYIPL PMFWALFDQQ
310 320 330 340 350
GSRWTLQATT MTGKIGTIEI QPDQMQTVNA ILIVIMVPIV DAVVYPLIAK
360 370 380 390 400
CGFNFTSLKK MTVGMFLASM AFVVAAIVQV EIDKTLPVFP SGNQVQIKVL
410 420 430 440 450
NIGNNDMAVY FPGKNVTVAQ MSQTDTFMTF DVDQLTSINV SSPGSPGVTT
460 470 480 490 500
VAHEFEPGHR HTLLVWGPNL YRVVKDGLNQ KPEKGENGIR FVSTLNEMIT
510 520 530 540 550
IKMSGKVYEN VTSHSASNYQ FFPSGQKDYT INTTEIAPNC SSDFKSSNLD
560 570 580 590 600
FGSAYTYVIR SRASDGCLEV KEFEDIPPNT VNMALQIPQY FLLTCGEVVF
610 620 630 640 650
SVTGLEFSYS QAPSNMKSVL QAGWLLTVAI GNIIVLIVAE AGHFDKQWAE
660 670 680 690 700
YVLFASLLLV VCIIFAIMAR FYTYINPAEI EAQFDEDEKK KGVGKENPYS
710
SLEPVSQTNM
Length:710
Mass (Da):78,929
Last modified:October 1, 1996 - v1
Checksum:i435727A6C76F2D7B
GO
Isoform 2 (identifier: P51574-2) [UniParc]FASTAAdd to basket

Also known as: pgPepT1

The sequence of this isoform differs from the canonical sequence as follows:
     1-560: Missing.
     561-563: SRA → MVQ

Show »
Length:150
Mass (Da):16,710
Checksum:i6A9C0D099A810073
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti241 – 2411R → G in BAA08844 (PubMed:8531138).Curated
Sequence conflicti259 – 2591N → E in BAA08844 (PubMed:8531138).Curated
Sequence conflicti279 – 2802IM → MV in BAA08844 (PubMed:8531138).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 560560Missing in isoform 2. 1 PublicationVSP_042081Add
BLAST
Alternative sequencei561 – 5633SRA → MVQ in isoform 2. 1 PublicationVSP_042082

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50664 mRNA. Translation: BAA09318.1.
D50306 mRNA. Translation: BAA08844.1.
AY860424 mRNA. Translation: AAW80389.1.
PIRiS72497.
RefSeqiNP_001073307.1. NM_001079838.1. [P51574-2]
NP_476462.1. NM_057121.1. [P51574-1]
UniGeneiRn.10500.

Genome annotation databases

GeneIDi117261.
KEGGirno:117261.

Keywords - Coding sequence diversityi

Alternative promoter usage

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50664 mRNA. Translation: BAA09318.1.
D50306 mRNA. Translation: BAA08844.1.
AY860424 mRNA. Translation: AAW80389.1.
PIRiS72497.
RefSeqiNP_001073307.1. NM_001079838.1. [P51574-2]
NP_476462.1. NM_057121.1. [P51574-1]
UniGeneiRn.10500.

3D structure databases

ProteinModelPortaliP51574.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000015890.

Chemistry

BindingDBiP51574.
ChEMBLiCHEMBL2073691.

Protein family/group databases

TCDBi2.A.17.4.1. the proton-dependent oligopeptide transporter (pot/ptr) family.

Proteomic databases

PaxDbiP51574.
PRIDEiP51574.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi117261.
KEGGirno:117261.

Organism-specific databases

CTDi6564.
RGDi621736. Slc15a1.

Phylogenomic databases

eggNOGiKOG1237. Eukaryota.
COG3104. LUCA.
HOGENOMiHOG000285186.
HOVERGENiHBG052962.
InParanoidiP51574.
KOiK14206.
PhylomeDBiP51574.

Miscellaneous databases

PROiP51574.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR004768. Oligopep_transport.
IPR000109. POT_fam.
IPR018456. PTR2_symporter_CS.
[Graphical view]
PANTHERiPTHR11654. PTHR11654. 2 hits.
PfamiPF00854. PTR2. 2 hits.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 2 hits.
TIGRFAMsiTIGR00926. 2A1704. 1 hit.
PROSITEiPS01022. PTR2_1. 1 hit.
PS01023. PTR2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Sequence, tissue distribution and developmental changes in rat intestinal oligopeptide transporter."
    Miyamoto K., Shiraga T., Morita K., Yamamoto H., Haga H., Taketani Y., Tamai I., Sai Y., Tsuji A., Takeda E.
    Biochim. Biophys. Acta 1305:34-38(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Small intestine.
  2. "Cloning and characterization of a rat H+/peptide cotransporter mediating absorption of beta-lactam antibiotics in the intestine and kidney."
    Saito H., Okuda M., Terada T., Sasaki S., Inui K.
    J. Pharmacol. Exp. Ther. 275:1631-1637(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  3. "A novel pineal-specific product of the oligopeptide transporter PepT1 gene: circadian expression mediated by cAMP activation of an intronic promoter."
    Gaildrat P., Moller M., Mukda S., Humphries A., Carter D.A., Ganapathy V., Klein D.C.
    J. Biol. Chem. 280:16851-16860(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), ALTERNATIVE PROMOTER USAGE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION.
  4. Cited for: TISSUE SPECIFICITY, INDUCTION.

Entry informationi

Entry nameiS15A1_RAT
AccessioniPrimary (citable) accession number: P51574
Secondary accession number(s): Q5EML1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.