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P51569

- AGAL_MOUSE

UniProt

P51569 - AGAL_MOUSE

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Protein

Alpha-galactosidase A

Gene

Gla

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei170 – 1701NucleophileBy similarity
Active sitei231 – 2311Proton donorBy similarity

GO - Molecular functioni

  1. alpha-galactosidase activity Source: UniProtKB
  2. catalytic activity Source: UniProtKB
  3. galactoside binding Source: Ensembl
  4. protein homodimerization activity Source: UniProtKB
  5. raffinose alpha-galactosidase activity Source: UniProtKB-EC
  6. receptor binding Source: UniProtKB

GO - Biological processi

  1. glycoside catabolic process Source: RefGenome
  2. glycosylceramide catabolic process Source: MGI
  3. negative regulation of nitric oxide biosynthetic process Source: UniProtKB
  4. negative regulation of nitric-oxide synthase activity Source: UniProtKB
  5. oligosaccharide metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

ReactomeiREACT_199008. Glycosphingolipid metabolism.

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase A (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactosidase A
Alpha-D-galactoside galactohydrolase
Melibiase
Gene namesi
Name:Gla
Synonyms:Ags
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:1347344. Gla.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. extracellular region Source: UniProtKB
  3. extracellular vesicular exosome Source: Ensembl
  4. Golgi apparatus Source: UniProtKB
  5. lysosome Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131By similarityAdd
BLAST
Chaini32 – 419388Alpha-galactosidase APRO_0000001005Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 94By similarity
Disulfide bondi56 ↔ 63By similarity
Glycosylationi139 – 1391N-linked (GlcNAc...)By similarity
Disulfide bondi142 ↔ 172By similarity
Modified residuei186 – 1861Phosphotyrosine1 Publication
Glycosylationi192 – 1921N-linked (GlcNAc...)By similarity
Disulfide bondi202 ↔ 223By similarity
Glycosylationi215 – 2151N-linked (GlcNAc...)By similarity
Disulfide bondi378 ↔ 382By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP51569.
PaxDbiP51569.
PRIDEiP51569.

PTM databases

PhosphoSiteiP51569.

Expressioni

Gene expression databases

BgeeiP51569.
CleanExiMM_GLA.
ExpressionAtlasiP51569. baseline and differential.
GenevestigatoriP51569.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

IntActiP51569. 1 interaction.
MINTiMINT-4087336.

Structurei

3D structure databases

ProteinModelPortaliP51569.
SMRiP51569. Positions 32-418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni203 – 2075Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG68897.
HOVERGENiHBG001989.
InParanoidiP51569.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02065. Melibiase. 1 hit.
[Graphical view]
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51569-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLLSRDTRL VCELALCPLA LVFWSILGVR ALDNGLARTP TMGWLHWERF
60 70 80 90 100
MCNLDCQEEP DACISEQLFM QMAELMVSDG WRDAGYDYLC IDDCWMAPER
110 120 130 140 150
DSKGRLQADP QRFPSGIKHL ANYVHSKGLK LGIYADVGNK TCAGFPGSFG
160 170 180 190 200
SYDIDAQTFA DWGVDLLKFD GCHCDSVVSL ENGYKYMALA LNRTGRSIVY
210 220 230 240 250
SCEWPLYLRP FHKPNYTDIQ YYCNHWRNFD DVYDSWESIK NILSWTVVYQ
260 270 280 290 300
KEIVEVAGPG SWNDPDMLVI GNFGLSWDQQ VTQMALWAIM AAPLLMSNDL
310 320 330 340 350
RQISSQAKAL LQNKDVIAIN QDPLGKQGYC FRKENHIEVW ERPLSNLAWA
360 370 380 390 400
VAVRNLQEIG GPCPYTIQIS SLGRGLACNP GCIITQLLPE KVHLGFYEWT
410
LTLKTRVNPS GTVLFRLER
Length:419
Mass (Da):47,643
Last modified:October 1, 1996 - v1
Checksum:iBD5E6A99AC113613
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34071 mRNA. Translation: AAA96749.1.
L46651 Genomic DNA. Translation: AAA74453.1.
U58105 Genomic DNA. Translation: AAB47244.1.
U50716 mRNA. Translation: AAC52584.1.
U50715 Genomic DNA. Translation: AAC52583.1.
BC009021 mRNA. Translation: AAH09021.1.
PIRiJC4522.
UniGeneiMm.1114.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U34071 mRNA. Translation: AAA96749.1 .
L46651 Genomic DNA. Translation: AAA74453.1 .
U58105 Genomic DNA. Translation: AAB47244.1 .
U50716 mRNA. Translation: AAC52584.1 .
U50715 Genomic DNA. Translation: AAC52583.1 .
BC009021 mRNA. Translation: AAH09021.1 .
PIRi JC4522.
UniGenei Mm.1114.

3D structure databases

ProteinModelPortali P51569.
SMRi P51569. Positions 32-418.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P51569. 1 interaction.
MINTi MINT-4087336.

Protein family/group databases

CAZyi GH27. Glycoside Hydrolase Family 27.

PTM databases

PhosphoSitei P51569.

Proteomic databases

MaxQBi P51569.
PaxDbi P51569.
PRIDEi P51569.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:1347344. Gla.

Phylogenomic databases

eggNOGi NOG68897.
HOVERGENi HBG001989.
InParanoidi P51569.

Enzyme and pathway databases

Reactomei REACT_199008. Glycosphingolipid metabolism.

Miscellaneous databases

PROi P51569.
SOURCEi Search...

Gene expression databases

Bgeei P51569.
CleanExi MM_GLA.
ExpressionAtlasi P51569. baseline and differential.
Genevestigatori P51569.

Family and domain databases

Gene3Di 2.60.40.1180. 1 hit.
3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR013780. Glyco_hydro_13_b.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_GHD.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF02065. Melibiase. 1 hit.
[Graphical view ]
PRINTSi PR00740. GLHYDRLASE27.
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structural organization and expression of the mouse gene encoding alpha-galactosidase A."
    Ohshima T., Murray G.J., Nagle J.W., Quirk J.M., Kraus M.H., Barton N.W., Brady R.O., Kulkarni A.B.
    Gene 166:277-280(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Kidney.
  2. "Sixty-nine kilobases of contiguous human genomic sequence containing the alpha-galactosidase A and Bruton's tyrosine kinase loci."
    Oeltjen J.C., Liu X., Lu J., Allen R.C., Muzny D.M., Belmont J.W., Gibbs R.A.
    Mamm. Genome 6:334-338(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: C129.
  3. "The entire genomic sequence and cDNA expression of mouse alpha-galactosidase A."
    Gotlib R.W., Bishop D.F., Wang A.M., Zeidner K.M., Ioannou Y.I., Adler D.A., Disteche C.M., Desnick R.J.
    Biochem. Mol. Med. 57:139-148(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-186, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.

Entry informationi

Entry nameiAGAL_MOUSE
AccessioniPrimary (citable) accession number: P51569
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3