Alpha-galactosidase A precursor - Mus musculus (Mouse)

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Reviewed, UniProtKB/Swiss-Prot P51569 (AGAL_MOUSE)

Last modified October 13, 2009. Version 72. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Alpha-galactosidase A
    EC=3.2.1.22
Alternative name(s):
    Alpha-D-galactoside galactohydrolase
    Alpha-D-galactosidase A
    Melibiase

Gene names

Name:	Gla
Synonyms:	Ags

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactohydrolase.
Subunit structure	Homodimer.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the glycosyl hydrolase 27 family.

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Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	glycosylceramide catabolic process Inferred from direct assay. Source: MGI negative regulation of nitric oxide biosynthetic process Inferred from mutant phenotype. Source: UniProtKB negative regulation of nitric-oxide synthase activity Inferred from mutant phenotype. Source: UniProtKB oligosaccharide metabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from sequence or structural similarity. Source: UniProtKB extracellular region Inferred from sequence or structural similarity. Source: UniProtKB lysosome Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	alpha-galactosidase activity Inferred from sequence or structural similarity. Source: UniProtKB cation binding Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB receptor binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 31

By similarity

Chain

32 – 419

388

Alpha-galactosidase A

PRO_0000001005

Regions

Region

203 – 207

Substrate binding By similarity

Sites

Active site

170

Nucleophile By similarity

Active site

231

Proton donor By similarity

Amino acid modifications

Modified residue

186

Phosphotyrosine Ref.5

Glycosylation

139

N-linked (GlcNAc...) By similarity

Glycosylation

192

N-linked (GlcNAc...) By similarity

Glycosylation

215

N-linked (GlcNAc...) By similarity

Disulfide bond

52 ↔ 94

By similarity

Disulfide bond

56 ↔ 63

By similarity

Disulfide bond

142 ↔ 172

By similarity

Disulfide bond

202 ↔ 223

By similarity

Disulfide bond

378 ↔ 382

By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P51569-1 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: BD5E6A99AC113613
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FASTA

419

47,643

        10         20         30         40         50         60 
MKLLSRDTRL VCELALCPLA LVFWSILGVR ALDNGLARTP TMGWLHWERF MCNLDCQEEP 

        70         80         90        100        110        120 
DACISEQLFM QMAELMVSDG WRDAGYDYLC IDDCWMAPER DSKGRLQADP QRFPSGIKHL 

       130        140        150        160        170        180 
ANYVHSKGLK LGIYADVGNK TCAGFPGSFG SYDIDAQTFA DWGVDLLKFD GCHCDSVVSL 

       190        200        210        220        230        240 
ENGYKYMALA LNRTGRSIVY SCEWPLYLRP FHKPNYTDIQ YYCNHWRNFD DVYDSWESIK 

       250        260        270        280        290        300 
NILSWTVVYQ KEIVEVAGPG SWNDPDMLVI GNFGLSWDQQ VTQMALWAIM AAPLLMSNDL 

       310        320        330        340        350        360 
RQISSQAKAL LQNKDVIAIN QDPLGKQGYC FRKENHIEVW ERPLSNLAWA VAVRNLQEIG 

       370        380        390        400        410 
GPCPYTIQIS SLGRGLACNP GCIITQLLPE KVHLGFYEWT LTLKTRVNPS GTVLFRLER

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural organization and expression of the mouse gene encoding alpha-galactosidase A." Ohshima T., Murray G.J., Nagle J.W., Quirk J.M., Kraus M.H., Barton N.W., Brady R.O., Kulkarni A.B. Gene 166:277-280(1995) [PubMed: 8543175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Kidney.
[2]	"Sixty-nine kilobases of contiguous human genomic sequence containing the alpha-galactosidase A and Bruton's tyrosine kinase loci." Oeltjen J.C., Liu X., Lu J., Allen R.C., Muzny D.M., Belmont J.W., Gibbs R.A. Mamm. Genome 6:334-338(1995) [PubMed: 7626884] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: C129.
[3]	"The entire genomic sequence and cDNA expression of mouse alpha-galactosidase A." Gotlib R.W., Bishop D.F., Wang A.M., Zeidner K.M., Ioannou Y.I., Adler D.A., Disteche C.M., Desnick R.J. Biochem. Mol. Med. 57:139-148(1996) [PubMed: 8733892] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[5]	"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain." Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P. J. Proteome Res. 7:311-318(2008) [PubMed: 18034455] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-186, MASS SPECTROMETRY. Tissue: Brain.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	U34071 mRNA. Translation: AAA96749.1. L46651 Genomic DNA. Translation: AAA74453.1. U58105 Genomic DNA. Translation: AAB47244.1. U50716 mRNA. Translation: AAC52584.1. U50715 Genomic DNA. Translation: AAC52583.1. BC009021 mRNA. Translation: AAH09021.1.
IPI	IPI00123814.
PIR	JC4522.
UniGene	Mm.1114
3D structure databases
SMR	P51569. Positions 32-418.
ModBase	Search...
Protein-protein interaction databases
STRING	P51569.
Protein family/group databases
CAZy	GH27. Glycoside Hydrolase Family 27.
PTM databases
PhosphoSite	P51569.
Proteomic databases
PRIDE	P51569.
Genome annotation databases
Ensembl	ENSMUST00000033621; ENSMUSP00000033621; ENSMUSG00000031266; Mus musculus. [Genome view]
UCSC	uc009ugg.1. mouse.
Organism-specific databases
MGI	MGI:1347344. Gla.
Phylogenomic databases
HOVERGEN	P51569.
Enzyme and pathway databases
BRENDA	3.2.1.22. 244.
Gene expression databases
ArrayExpress	P51569.
Bgee	P51569.
CleanEx	MM_GLA.
Genevestigator	P51569.
GermOnline	ENSMUSG00000031266. Mus musculus.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR013780. Glyco_hydro_13_b. IPR002241. Glyco_hydro_27. IPR000111. Glyco_hydro_GHD. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit. G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit.
Pfam	PF02065. Melibiase. 1 hit. [Graphical view]
PRINTS	PR00740. GLHYDRLASE27.
ProDom	PD002572. Glyco_hydro_GHD. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00512. ALPHA_GALACTOSIDASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

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Entry information

Entry name

AGAL_MOUSE

Accession

Primary (citable) accession number: P51569

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents