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P51569

- AGAL_MOUSE

UniProt

P51569 - AGAL_MOUSE

Protein

Alpha-galactosidase A

Gene

Gla

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei170 – 1701NucleophileBy similarity
    Active sitei231 – 2311Proton donorBy similarity

    GO - Molecular functioni

    1. alpha-galactosidase activity Source: UniProtKB
    2. catalytic activity Source: UniProtKB
    3. galactoside binding Source: Ensembl
    4. protein homodimerization activity Source: UniProtKB
    5. raffinose alpha-galactosidase activity Source: UniProtKB-EC
    6. receptor binding Source: UniProtKB

    GO - Biological processi

    1. glycoside catabolic process Source: RefGenome
    2. glycosylceramide catabolic process Source: MGI
    3. negative regulation of nitric oxide biosynthetic process Source: UniProtKB
    4. negative regulation of nitric-oxide synthase activity Source: UniProtKB
    5. oligosaccharide metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    ReactomeiREACT_199008. Glycosphingolipid metabolism.

    Protein family/group databases

    CAZyiGH27. Glycoside Hydrolase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-galactosidase A (EC:3.2.1.22)
    Alternative name(s):
    Alpha-D-galactosidase A
    Alpha-D-galactoside galactohydrolase
    Melibiase
    Gene namesi
    Name:Gla
    Synonyms:Ags
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:1347344. Gla.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. extracellular region Source: UniProtKB
    3. Golgi apparatus Source: UniProtKB
    4. lysosome Source: UniProtKB

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3131By similarityAdd
    BLAST
    Chaini32 – 419388Alpha-galactosidase APRO_0000001005Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi52 ↔ 94By similarity
    Disulfide bondi56 ↔ 63By similarity
    Glycosylationi139 – 1391N-linked (GlcNAc...)By similarity
    Disulfide bondi142 ↔ 172By similarity
    Modified residuei186 – 1861Phosphotyrosine1 Publication
    Glycosylationi192 – 1921N-linked (GlcNAc...)By similarity
    Disulfide bondi202 ↔ 223By similarity
    Glycosylationi215 – 2151N-linked (GlcNAc...)By similarity
    Disulfide bondi378 ↔ 382By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP51569.
    PaxDbiP51569.
    PRIDEiP51569.

    PTM databases

    PhosphoSiteiP51569.

    Expressioni

    Gene expression databases

    ArrayExpressiP51569.
    BgeeiP51569.
    CleanExiMM_GLA.
    GenevestigatoriP51569.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    IntActiP51569. 1 interaction.
    MINTiMINT-4087336.

    Structurei

    3D structure databases

    ProteinModelPortaliP51569.
    SMRiP51569. Positions 32-418.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni203 – 2075Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 27 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG68897.
    HOVERGENiHBG001989.
    InParanoidiP51569.

    Family and domain databases

    Gene3Di2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02065. Melibiase. 1 hit.
    [Graphical view]
    PRINTSiPR00740. GLHYDRLASE27.
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P51569-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKLLSRDTRL VCELALCPLA LVFWSILGVR ALDNGLARTP TMGWLHWERF    50
    MCNLDCQEEP DACISEQLFM QMAELMVSDG WRDAGYDYLC IDDCWMAPER 100
    DSKGRLQADP QRFPSGIKHL ANYVHSKGLK LGIYADVGNK TCAGFPGSFG 150
    SYDIDAQTFA DWGVDLLKFD GCHCDSVVSL ENGYKYMALA LNRTGRSIVY 200
    SCEWPLYLRP FHKPNYTDIQ YYCNHWRNFD DVYDSWESIK NILSWTVVYQ 250
    KEIVEVAGPG SWNDPDMLVI GNFGLSWDQQ VTQMALWAIM AAPLLMSNDL 300
    RQISSQAKAL LQNKDVIAIN QDPLGKQGYC FRKENHIEVW ERPLSNLAWA 350
    VAVRNLQEIG GPCPYTIQIS SLGRGLACNP GCIITQLLPE KVHLGFYEWT 400
    LTLKTRVNPS GTVLFRLER 419
    Length:419
    Mass (Da):47,643
    Last modified:October 1, 1996 - v1
    Checksum:iBD5E6A99AC113613
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34071 mRNA. Translation: AAA96749.1.
    L46651 Genomic DNA. Translation: AAA74453.1.
    U58105 Genomic DNA. Translation: AAB47244.1.
    U50716 mRNA. Translation: AAC52584.1.
    U50715 Genomic DNA. Translation: AAC52583.1.
    BC009021 mRNA. Translation: AAH09021.1.
    PIRiJC4522.
    UniGeneiMm.1114.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U34071 mRNA. Translation: AAA96749.1 .
    L46651 Genomic DNA. Translation: AAA74453.1 .
    U58105 Genomic DNA. Translation: AAB47244.1 .
    U50716 mRNA. Translation: AAC52584.1 .
    U50715 Genomic DNA. Translation: AAC52583.1 .
    BC009021 mRNA. Translation: AAH09021.1 .
    PIRi JC4522.
    UniGenei Mm.1114.

    3D structure databases

    ProteinModelPortali P51569.
    SMRi P51569. Positions 32-418.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P51569. 1 interaction.
    MINTi MINT-4087336.

    Protein family/group databases

    CAZyi GH27. Glycoside Hydrolase Family 27.

    PTM databases

    PhosphoSitei P51569.

    Proteomic databases

    MaxQBi P51569.
    PaxDbi P51569.
    PRIDEi P51569.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:1347344. Gla.

    Phylogenomic databases

    eggNOGi NOG68897.
    HOVERGENi HBG001989.
    InParanoidi P51569.

    Enzyme and pathway databases

    Reactomei REACT_199008. Glycosphingolipid metabolism.

    Miscellaneous databases

    PROi P51569.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P51569.
    Bgeei P51569.
    CleanExi MM_GLA.
    Genevestigatori P51569.

    Family and domain databases

    Gene3Di 2.60.40.1180. 1 hit.
    3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR013780. Glyco_hydro_13_b.
    IPR002241. Glyco_hydro_27.
    IPR000111. Glyco_hydro_GHD.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02065. Melibiase. 1 hit.
    [Graphical view ]
    PRINTSi PR00740. GLHYDRLASE27.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00512. ALPHA_GALACTOSIDASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural organization and expression of the mouse gene encoding alpha-galactosidase A."
      Ohshima T., Murray G.J., Nagle J.W., Quirk J.M., Kraus M.H., Barton N.W., Brady R.O., Kulkarni A.B.
      Gene 166:277-280(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Kidney.
    2. "Sixty-nine kilobases of contiguous human genomic sequence containing the alpha-galactosidase A and Bruton's tyrosine kinase loci."
      Oeltjen J.C., Liu X., Lu J., Allen R.C., Muzny D.M., Belmont J.W., Gibbs R.A.
      Mamm. Genome 6:334-338(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: C129.
    3. "The entire genomic sequence and cDNA expression of mouse alpha-galactosidase A."
      Gotlib R.W., Bishop D.F., Wang A.M., Zeidner K.M., Ioannou Y.I., Adler D.A., Disteche C.M., Desnick R.J.
      Biochem. Mol. Med. 57:139-148(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    5. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
      Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
      J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-186, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Brain.

    Entry informationi

    Entry nameiAGAL_MOUSE
    AccessioniPrimary (citable) accession number: P51569
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3