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Reviewed, UniProtKB/Swiss-Prot P51568 (AFC3_ARATH)

Last modified June 16, 2009. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Serine/threonine-protein kinase AFC3
    EC=2.7.12.1
Gene names
Name: AFC3
Synonyms: AME3
Ordered Locus Names: At4g32660
ORF Names: F4D11.140
OrganismArabidopsis thaliana (Mouse-ear cress) [Complete proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IIBrassicalesBrassicaceaeArabidopsis

Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily.

Contains 1 protein kinase domain.

Ontologies

Keywords
   Coding sequence diversityAlternative splicing
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processprotein amino acid phosphorylation

Inferred from electronic annotation. Source: InterPro

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein serine/threonine kinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: P51568-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Serine/threonine-protein kinase AFC3
PRO_0000085602

Regions

Domain71 – 396326Protein kinase
Nucleotide binding77 – 859ATP By similarity

Sites

Active site1961Proton acceptor By similarity
Binding site1001ATP By similarity

Experimental info

Sequence conflict81S → N in AAA57119. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 8F707ABDCF9B1352

FASTA40046,224
        10         20         30         40         50         60 
MIANGFESMD KERVRKRPRM TWDEAPAEPE AKRAVIKGHG SDGRILSPPL RDDDRDGHYV 

        70         80         90        100        110        120 
FSLRDNLTPR YKILSKMGEG TFGRVLECWD RDTKEYVAIK IIRSIKKYRD AAMIEIDVLQ 

       130        140        150        160        170        180 
KLVKSDKGRT RCVQMKNWFD YRNHICIVFE KLGPSLFDFL KRNKYSAFPL ALVRDFGCQL 

       190        200        210        220        230        240 
LESVAYMHEL QLVHTDLKPE NILLVSSENV KLPDNKRSAA NETHFRCLPK SSAIKLIDFG 

       250        260        270        280        290        300 
STVCDNRIHH SIVQTRHYRS PEVILGLGWS YQCDLWSIGC ILFELCTGEA LFQTHDNLEH 

       310        320        330        340        350        360 
LAMMERALGP LPEHMTRKAS RGAEKYFRRG CRLNWPEGAN SRESIRAVKR LDRLKDMVSK 

       370        380        390        400 
HVDNTRSRFA DLLYGLLAYD PSERLTANEA LDHPFFKSSS 

« Hide

References

« Hide 'large scale' references
[1]"A.thaliana genes encoding protein kinases of a new family."
Kuromori T., Yamamoto M.
Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana."
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. expand/collapse author list , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.
Nature 402:769-777(1999) [PubMed: 10617198] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]"AFC1, a LAMMER kinase from Arabidopsis thaliana, activates STE12-dependent processes in yeast."
Bender J., Fink G.R.
Proc. Natl. Acad. Sci. U.S.A. 91:12105-12109(1994) [PubMed: 7991592] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-400.
Strain: cv. Landsberg erecta.

Cross-references

Sequence databases

D45355 mRNA. Translation: BAA08216.1.
AL022537 Genomic DNA. Translation: CAA18595.1.
AL161581 Genomic DNA. Translation: CAB79983.1.
U16178 mRNA. Translation: AAA57119.1.
IPIIPI00548682.
PIRT04460.
RefSeqNP_194992.1.
UniGeneAt.21547

3D structure databases

HSSPHSSP built from PDB template 1HOW based on UniProtKB Q03656.
ModBaseSearch...

Proteomic databases

PRIDEP51568.

Genome annotation databases

GeneID829401.
GenomeReviewsGene locus AT4G32660 in contig CT486007_GR.

Organism-specific databases

TAIRAt4g32660.

Phylogenomic databases

OMAP51568. VAYMHEL.

Enzyme and pathway databases

BRENDA2.7.12.1. 302.

Gene expression databases

ArrayExpressP51568.

Family and domain databases

InterProIPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. False negative.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAFC3_ARATH
AccessionPrimary (citable) accession number: P51568
Secondary accession number(s): Q39185
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 15, 1998
Last modified: June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents