Reviewed, UniProtKB/Swiss-Prot P51568 (AFC3_ARATH)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase AFC3
    EC=2.7.12.1

Gene names

Name:	AFC3
Synonyms:	AME3
Ordered Locus Names:	At4g32660
ORF Names:	F4D11.140

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P51568-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Serine/threonine-protein kinase AFC3		PRO_0000085602
Regions
	Domain	71 – 396	326	Protein kinase
	Nucleotide binding	77 – 85	9	ATP By similarity
Sites
	Active site	196	1	Proton acceptor By similarity
	Binding site	100	1	ATP By similarity
Experimental info
	Sequence conflict	8	1	S → N in AAA57119. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 8F707ABDCF9B1352	FASTA	400	46,224
	10 20 30 40 50 60 MIANGFESMD KERVRKRPRM TWDEAPAEPE AKRAVIKGHG SDGRILSPPL RDDDRDGHYV 70 80 90 100 110 120 FSLRDNLTPR YKILSKMGEG TFGRVLECWD RDTKEYVAIK IIRSIKKYRD AAMIEIDVLQ 130 140 150 160 170 180 KLVKSDKGRT RCVQMKNWFD YRNHICIVFE KLGPSLFDFL KRNKYSAFPL ALVRDFGCQL 190 200 210 220 230 240 LESVAYMHEL QLVHTDLKPE NILLVSSENV KLPDNKRSAA NETHFRCLPK SSAIKLIDFG 250 260 270 280 290 300 STVCDNRIHH SIVQTRHYRS PEVILGLGWS YQCDLWSIGC ILFELCTGEA LFQTHDNLEH 310 320 330 340 350 360 LAMMERALGP LPEHMTRKAS RGAEKYFRRG CRLNWPEGAN SRESIRAVKR LDRLKDMVSK 370 380 390 400 HVDNTRSRFA DLLYGLLAYD PSERLTANEA LDHPFFKSSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A.thaliana genes encoding protein kinases of a new family." Kuromori T., Yamamoto M. Submitted (FEB-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed: 10617198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"AFC1, a LAMMER kinase from Arabidopsis thaliana, activates STE12-dependent processes in yeast." Bender J., Fink G.R. Proc. Natl. Acad. Sci. U.S.A. 91:12105-12109(1994) [PubMed: 7991592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 6-400. Strain: cv. Landsberg erecta.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D45355 mRNA. Translation: BAA08216.1. AL022537 Genomic DNA. Translation: CAA18595.1. AL161581 Genomic DNA. Translation: CAB79983.1. U16178 mRNA. Translation: AAA57119.1.
IPI	IPI00548682.
PIR	T04460.
RefSeq	NP_194992.1.
UniGene	At.21547
3D structure databases
HSSP	HSSP built from PDB template 1HOW based on UniProtKB Q03656.
ModBase	Search...
Proteomic databases
PRIDE	P51568.
Genome annotation databases
GeneID	829401.
GenomeReviews	Gene locus AT4G32660 in contig CT486007_GR.
Organism-specific databases
TAIR	At4g32660.
Phylogenomic databases
OMA	P51568. VAYMHEL.
Enzyme and pathway databases
BRENDA	2.7.12.1. 302.
Gene expression databases
ArrayExpress	P51568.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFC3_ARATH

Accession

Primary (citable) accession number: P51568
Secondary accession number(s): Q39185

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 15, 1998
Last modified:	June 16, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)