Reviewed, UniProtKB/Swiss-Prot P51566 (AFC1_ARATH)

Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase AFC1
    EC=2.7.12.1

Gene names

Name:	AFC1
Synonyms:	AME2
Ordered Locus Names:	At3g53570
ORF Names:	F4P12_270

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activator of yeast transcription factor, STE12.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Lammer subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro regulation of transcription Ref.1 Traceable author statement. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: P51566-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Serine/threonine-protein kinase AFC1		PRO_0000085600
Regions
	Domain	115 – 443	329	Protein kinase
	Nucleotide binding	121 – 129	9	ATP By similarity
Sites
	Active site	240	1	Proton acceptor By similarity
	Binding site	144	1	ATP By similarity
Experimental info
	Sequence conflict	117	1	I → M in AAA57117. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified December 6, 2002. Version 2. Checksum: A885FD32CE11B181	FASTA	467	54,198
	10 20 30 40 50 60 MQSSVYRDKA SSIAMILETQ RNVEFPHRIV DKRPRKRPRL TWDAAPPLLP PPPPPTVFQP 70 80 90 100 110 120 PLYYGPEFAS GLVPNFVYPN MFYNGLPRQG SPPWRPDDKD GHYVFVVGDT LTPRYQILSK 130 140 150 160 170 180 MGEGTFGQVL ECFDNKNKEV VAIKVIRSIN KYREAAMIEI DVLQRLTRHD VGGSRCVQIR 190 200 210 220 230 240 NWFDYRNHIC IVFEKLGPSL YDFLRKNSYR SFPIDLVREL GRQLLESVAY MHDLRLIHTD 250 260 270 280 290 300 LKPENILLVS SEYIKIPDYK FLSRPTKDGS YFKNLPKSSA IKLIDFGSTT FEHQDHNYIV 310 320 330 340 350 360 STRHYRAPEV ILGVGWNYPC DLWSIGCILV ELCSGEALFQ THENLEHLAM MERVLGPLPP 370 380 390 400 410 420 HMVLRADRRS EKYFRRGAKL DWPEGATSRD SLKAVWKLPR LPNLIMQHVD HSAGDLIDLL 430 440 450 460 QGLLRYDPTE RFKAREALNH PFFTRSREQS IPPFNPNPHP FLYNQKN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AFC1, a LAMMER kinase from Arabidopsis thaliana, activates STE12-dependent processes in yeast." Bender J., Fink G.R. Proc. Natl. Acad. Sci. U.S.A. 91:12105-12109(1994) [PubMed: 7991592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Landsberg erecta.
[2]	"A.thaliana genes encoding protein kinases of a new family." Kuromori T., Yamamoto M. Submitted (JAN-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed: 11130713] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U16176 mRNA. Translation: AAA57117.1. D45354 mRNA. Translation: BAA08215.1. AL132966 Genomic DNA. Translation: CAB67664.1.
IPI	IPI00525997.
PIR	S71169.
RefSeq	NP_001030853.1. NP_190925.1. NP_850695.2.
UniGene	At.277
3D structure databases
HSSP	HSSP built from PDB template 1H27 based on UniProtKB P24941.
ModBase	Search...
Proteomic databases
PRIDE	P51566.
Genome annotation databases
GeneID	824525.
GenomeReviews	Gene locus AT3G53570 in contig BA000014_GR.
KEGG	ath:AT3G53570.
Organism-specific databases
TAIR	At3g53570.
Phylogenomic databases
OMA	P51566. KYVRENC.
Enzyme and pathway databases
BRENDA	2.7.12.1. 302.
Gene expression databases
ArrayExpress	P51566.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AFC1_ARATH

Accession

Primary (citable) accession number: P51566
Secondary accession number(s): Q39184

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 6, 2002
Last modified:	June 16, 2009
This is version 64 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)