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P51556

- DGKA_RAT

UniProt

P51556 - DGKA_RAT

Protein

Diacylglycerol kinase alpha

Gene

Dgka

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.

    Catalytic activityi

    ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

    Enzyme regulationi

    Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Calcium bindingi121 – 132121CuratedAdd
    BLAST
    Calcium bindingi166 – 177122CuratedAdd
    BLAST
    Zinc fingeri203 – 25149Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri267 – 31751Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium ion binding Source: InterPro
    3. diacylglycerol kinase activity Source: UniProtKB-EC
    4. NAD+ kinase activity Source: InterPro

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diacylglycerol kinase alpha (EC:2.7.1.107)
    Short name:
    DAG kinase alpha
    Alternative name(s):
    80 kDa diacylglycerol kinase
    Diglyceride kinase alpha
    Short name:
    DGK-alpha
    Gene namesi
    Name:Dgka
    Synonyms:Dagk, Dagk1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi70904. Dgka.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 727727Diacylglycerol kinase alphaPRO_0000218456Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei476 – 4761N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP51556.
    PRIDEiP51556.

    PTM databases

    PhosphoSiteiP51556.

    Expressioni

    Tissue specificityi

    Lymphocytes and oligodendroglial cells.

    Gene expression databases

    GenevestigatoriP51556.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000038628.

    Structurei

    3D structure databases

    ProteinModelPortaliP51556.
    SMRiP51556. Positions 1-116.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini108 – 14336EF-hand 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini153 – 18836EF-hand 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini365 – 498134DAGKcPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 DAGKc domain.PROSITE-ProRule annotation
    Contains 2 EF-hand domains.PROSITE-ProRule annotation
    Contains 2 phorbol-ester/DAG-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri203 – 25149Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri267 – 31751Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47311.
    HOGENOMiHOG000252931.
    HOVERGENiHBG051345.
    InParanoidiP51556.
    KOiK00901.
    PhylomeDBiP51556.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view]
    PfamiPF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 2 hits.
    PROSITEiPS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51556-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKDKGLISP EDFAQLQKYI DYSTKSVSDV LKVFEMNKYC QGDEIGYLGF    50
    EQFLKMYLEV EEVPHHLCWT LFWSFHSSQD LDEETESKAN VICLSDVYCY 100
    FTLLEGGSPE DKLEFTFKLY DMDRNGILDS TEVEKIILQM MRVAEYLDWD 150
    VSELRPILQE MMKEMDRDGS GCVSLAEWVR AGATTVPLLV LLGIDMTMKD 200
    DGHHIWRPKR FSRPVYCNLC ELSIGLGKQG LSCNLCKYIV HDHCAMKAQP 250
    CEVSTYAKSR KDIGVQPHVW VRGGCHSGRC DRCQKKIRTY HSLTGLHCVW 300
    CHLEIHDDCL QAVGPECDCG LLRDHILPPC SIYPRVLVSG QECKQKTTDV 350
    TSLCTPEAFR IEPVSNTHPL LVFINPKSGG KQGQSVLWKF QYILNPRQVF 400
    NLKDGPEPGL RFFKDVPQFR VLVCGGDGTV GWILETIDKA NFPIVPPVAV 450
    LPLGTGNDLA RCLRWGRGYE GENLRKILKD IEISKVVYLD RWLLEVIPQQ 500
    NGEKSDPVPS QIINNYFSIG VDASIAHRFH LMREKYPEKF NSRMKNKLWY 550
    LEFATSESIF STCKKLEESV TVEICGKLLD LSDLSLEGIA VLNIPSMHGG 600
    SNLWGDTKRP HGDTCGINQA LGSVAKIITD PDILKTCVPD MSDKRLEVVG 650
    IEGVIEMGQI YTRLKSAGHR LAKCSEITFQ TTKTLPMQVD GEPWMQAPCT 700
    IKITHKNQMP MLMGPAPSSS NFFGFWS 727
    Length:727
    Mass (Da):82,199
    Last modified:October 1, 1996 - v1
    Checksum:iB5A248ADD2F61C1D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S49760 mRNA. Translation: AAB24434.1.
    PIRiA56879.
    RefSeqiNP_542965.1. NM_080787.1.
    UniGeneiRn.1840.

    Genome annotation databases

    GeneIDi140866.
    KEGGirno:140866.
    UCSCiRGD:70904. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S49760 mRNA. Translation: AAB24434.1 .
    PIRi A56879.
    RefSeqi NP_542965.1. NM_080787.1.
    UniGenei Rn.1840.

    3D structure databases

    ProteinModelPortali P51556.
    SMRi P51556. Positions 1-116.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000038628.

    PTM databases

    PhosphoSitei P51556.

    Proteomic databases

    PaxDbi P51556.
    PRIDEi P51556.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 140866.
    KEGGi rno:140866.
    UCSCi RGD:70904. rat.

    Organism-specific databases

    CTDi 1606.
    RGDi 70904. Dgka.

    Phylogenomic databases

    eggNOGi NOG47311.
    HOGENOMi HOG000252931.
    HOVERGENi HBG051345.
    InParanoidi P51556.
    KOi K00901.
    PhylomeDBi P51556.

    Miscellaneous databases

    NextBioi 620731.

    Gene expression databases

    Genevestigatori P51556.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.238.110. 1 hit.
    InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR029477. DAG_kinase_typeI_N.
    IPR000756. Diacylglycerol_kin_accessory.
    IPR001206. Diacylglycerol_kinase_cat_dom.
    IPR011992. EF-hand-dom_pair.
    IPR018247. EF_Hand_1_Ca_BS.
    IPR002048. EF_hand_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    [Graphical view ]
    Pfami PF00130. C1_1. 2 hits.
    PF14513. DAG_kinase_N. 1 hit.
    PF00609. DAGK_acc. 1 hit.
    PF00781. DAGK_cat. 1 hit.
    PF13499. EF-hand_7. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 2 hits.
    SM00045. DAGKa. 1 hit.
    SM00046. DAGKc. 1 hit.
    SM00054. EFh. 2 hits.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 2 hits.
    PROSITEi PS50146. DAGK. 1 hit.
    PS00018. EF_HAND_1. 2 hits.
    PS50222. EF_HAND_2. 2 hits.
    PS00479. ZF_DAG_PE_1. 2 hits.
    PS50081. ZF_DAG_PE_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Gene cloning, sequence, expression and in situ localization of 80 kDa diacylglycerol kinase specific to oligodendrocyte of rat brain."
      Goto K., Watanabe M., Kondo H., Yuasa H., Sakane F., Kanoh H.
      Brain Res. Mol. Brain Res. 16:75-87(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiDGKA_RAT
    AccessioniPrimary (citable) accession number: P51556
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3