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P51556

- DGKA_RAT

UniProt

P51556 - DGKA_RAT

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Protein
Diacylglycerol kinase alpha
Gene
Dgka, Dagk, Dagk1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi121 – 132121 Inferred
Add
BLAST
Calcium bindingi166 – 177122 Inferred
Add
BLAST
Zinc fingeri203 – 25149Phorbol-ester/DAG-type 1
Add
BLAST
Zinc fingeri267 – 31751Phorbol-ester/DAG-type 2
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. NAD+ kinase activity Source: InterPro
  3. calcium ion binding Source: InterPro
  4. diacylglycerol kinase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. protein kinase C-activating G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase alpha (EC:2.7.1.107)
Short name:
DAG kinase alpha
Alternative name(s):
80 kDa diacylglycerol kinase
Diglyceride kinase alpha
Short name:
DGK-alpha
Gene namesi
Name:Dgka
Synonyms:Dagk, Dagk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi70904. Dgka.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 727727Diacylglycerol kinase alpha
PRO_0000218456Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei476 – 4761N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP51556.
PRIDEiP51556.

PTM databases

PhosphoSiteiP51556.

Expressioni

Tissue specificityi

Lymphocytes and oligodendroglial cells.

Gene expression databases

GenevestigatoriP51556.

Interactioni

Subunit structurei

Monomer By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038628.

Structurei

3D structure databases

ProteinModelPortaliP51556.
SMRiP51556. Positions 1-116.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini108 – 14336EF-hand 1
Add
BLAST
Domaini153 – 18836EF-hand 2
Add
BLAST
Domaini365 – 498134DAGKc
Add
BLAST

Sequence similaritiesi

Contains 1 DAGKc domain.
Contains 2 EF-hand domains.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47311.
HOGENOMiHOG000252931.
HOVERGENiHBG051345.
InParanoidiP51556.
KOiK00901.
PhylomeDBiP51556.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProiIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
PfamiPF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 2 hits.
PROSITEiPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51556-1 [UniParc]FASTAAdd to Basket

« Hide

MAKDKGLISP EDFAQLQKYI DYSTKSVSDV LKVFEMNKYC QGDEIGYLGF    50
EQFLKMYLEV EEVPHHLCWT LFWSFHSSQD LDEETESKAN VICLSDVYCY 100
FTLLEGGSPE DKLEFTFKLY DMDRNGILDS TEVEKIILQM MRVAEYLDWD 150
VSELRPILQE MMKEMDRDGS GCVSLAEWVR AGATTVPLLV LLGIDMTMKD 200
DGHHIWRPKR FSRPVYCNLC ELSIGLGKQG LSCNLCKYIV HDHCAMKAQP 250
CEVSTYAKSR KDIGVQPHVW VRGGCHSGRC DRCQKKIRTY HSLTGLHCVW 300
CHLEIHDDCL QAVGPECDCG LLRDHILPPC SIYPRVLVSG QECKQKTTDV 350
TSLCTPEAFR IEPVSNTHPL LVFINPKSGG KQGQSVLWKF QYILNPRQVF 400
NLKDGPEPGL RFFKDVPQFR VLVCGGDGTV GWILETIDKA NFPIVPPVAV 450
LPLGTGNDLA RCLRWGRGYE GENLRKILKD IEISKVVYLD RWLLEVIPQQ 500
NGEKSDPVPS QIINNYFSIG VDASIAHRFH LMREKYPEKF NSRMKNKLWY 550
LEFATSESIF STCKKLEESV TVEICGKLLD LSDLSLEGIA VLNIPSMHGG 600
SNLWGDTKRP HGDTCGINQA LGSVAKIITD PDILKTCVPD MSDKRLEVVG 650
IEGVIEMGQI YTRLKSAGHR LAKCSEITFQ TTKTLPMQVD GEPWMQAPCT 700
IKITHKNQMP MLMGPAPSSS NFFGFWS 727
Length:727
Mass (Da):82,199
Last modified:October 1, 1996 - v1
Checksum:iB5A248ADD2F61C1D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S49760 mRNA. Translation: AAB24434.1.
PIRiA56879.
RefSeqiNP_542965.1. NM_080787.1.
UniGeneiRn.1840.

Genome annotation databases

GeneIDi140866.
KEGGirno:140866.
UCSCiRGD:70904. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S49760 mRNA. Translation: AAB24434.1 .
PIRi A56879.
RefSeqi NP_542965.1. NM_080787.1.
UniGenei Rn.1840.

3D structure databases

ProteinModelPortali P51556.
SMRi P51556. Positions 1-116.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000038628.

PTM databases

PhosphoSitei P51556.

Proteomic databases

PaxDbi P51556.
PRIDEi P51556.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 140866.
KEGGi rno:140866.
UCSCi RGD:70904. rat.

Organism-specific databases

CTDi 1606.
RGDi 70904. Dgka.

Phylogenomic databases

eggNOGi NOG47311.
HOGENOMi HOG000252931.
HOVERGENi HBG051345.
InParanoidi P51556.
KOi K00901.
PhylomeDBi P51556.

Miscellaneous databases

NextBioi 620731.

Gene expression databases

Genevestigatori P51556.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.238.110. 1 hit.
InterProi IPR016064. ATP-NAD_kinase_PpnK-typ.
IPR029477. DAG_kinase_typeI_N.
IPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view ]
Pfami PF00130. C1_1. 2 hits.
PF14513. DAG_kinase_N. 1 hit.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view ]
SUPFAMi SSF111331. SSF111331. 2 hits.
PROSITEi PS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Gene cloning, sequence, expression and in situ localization of 80 kDa diacylglycerol kinase specific to oligodendrocyte of rat brain."
    Goto K., Watanabe M., Kondo H., Yuasa H., Sakane F., Kanoh H.
    Brain Res. Mol. Brain Res. 16:75-87(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiDGKA_RAT
AccessioniPrimary (citable) accession number: P51556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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