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Protein

Diacylglycerol kinase alpha

Gene

Dgka

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulationi

Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi121 – 1321CuratedAdd BLAST12
Calcium bindingi166 – 1772CuratedAdd BLAST12
Zinc fingeri203 – 251Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri267 – 317Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Calcium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.1.107 5301
ReactomeiR-RNO-114508 Effects of PIP2 hydrolysis

Chemistry databases

SwissLipidsiSLP:000000738

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase alpha (EC:2.7.1.107)
Short name:
DAG kinase alpha
Alternative name(s):
80 kDa diacylglycerol kinase
Diglyceride kinase alpha
Short name:
DGK-alpha
Gene namesi
Name:Dgka
Synonyms:Dagk, Dagk1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi70904 Dgka

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002184561 – 727Diacylglycerol kinase alphaAdd BLAST727

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei476N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP51556
PRIDEiP51556

PTM databases

PhosphoSitePlusiP51556

Expressioni

Tissue specificityi

Lymphocytes and oligodendroglial cells.

Gene expression databases

BgeeiENSRNOG00000022943
GenevisibleiP51556 RN

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000038628

Structurei

3D structure databases

ProteinModelPortaliP51556
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini108 – 143EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini153 – 188EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini365 – 498DAGKcPROSITE-ProRule annotationAdd BLAST134

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri203 – 251Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST49
Zinc fingeri267 – 317Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1169 Eukaryota
ENOG410XQVB LUCA
GeneTreeiENSGT00760000119050
HOGENOMiHOG000252931
HOVERGENiHBG051345
InParanoidiP51556
KOiK00901
OMAiDGQHLWR
OrthoDBiEOG091G04A5
PhylomeDBiP51556

Family and domain databases

CDDicd00029 C1, 1 hit
cd00051 EFh, 1 hit
Gene3Di1.10.238.110, 1 hit
3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR029477 DAG_kinase_typeI_N
IPR037607 DGK
IPR038199 DGK_typeI_N_sf
IPR000756 Diacylglycerol_kin_accessory
IPR001206 Diacylglycerol_kinase_cat_dom
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR016064 NAD/diacylglycerol_kinase_sf
IPR002219 PE/DAG-bd
PANTHERiPTHR11255 PTHR11255, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF14513 DAG_kinase_N, 1 hit
PF00609 DAGK_acc, 1 hit
PF00781 DAGK_cat, 1 hit
PF13499 EF-hand_7, 1 hit
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00045 DAGKa, 1 hit
SM00046 DAGKc, 1 hit
SM00054 EFh, 2 hits
SUPFAMiSSF111331 SSF111331, 2 hits
SSF47473 SSF47473, 2 hits
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 2 hits
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequencei

Sequence statusi: Complete.

P51556-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKDKGLISP EDFAQLQKYI DYSTKSVSDV LKVFEMNKYC QGDEIGYLGF
60 70 80 90 100
EQFLKMYLEV EEVPHHLCWT LFWSFHSSQD LDEETESKAN VICLSDVYCY
110 120 130 140 150
FTLLEGGSPE DKLEFTFKLY DMDRNGILDS TEVEKIILQM MRVAEYLDWD
160 170 180 190 200
VSELRPILQE MMKEMDRDGS GCVSLAEWVR AGATTVPLLV LLGIDMTMKD
210 220 230 240 250
DGHHIWRPKR FSRPVYCNLC ELSIGLGKQG LSCNLCKYIV HDHCAMKAQP
260 270 280 290 300
CEVSTYAKSR KDIGVQPHVW VRGGCHSGRC DRCQKKIRTY HSLTGLHCVW
310 320 330 340 350
CHLEIHDDCL QAVGPECDCG LLRDHILPPC SIYPRVLVSG QECKQKTTDV
360 370 380 390 400
TSLCTPEAFR IEPVSNTHPL LVFINPKSGG KQGQSVLWKF QYILNPRQVF
410 420 430 440 450
NLKDGPEPGL RFFKDVPQFR VLVCGGDGTV GWILETIDKA NFPIVPPVAV
460 470 480 490 500
LPLGTGNDLA RCLRWGRGYE GENLRKILKD IEISKVVYLD RWLLEVIPQQ
510 520 530 540 550
NGEKSDPVPS QIINNYFSIG VDASIAHRFH LMREKYPEKF NSRMKNKLWY
560 570 580 590 600
LEFATSESIF STCKKLEESV TVEICGKLLD LSDLSLEGIA VLNIPSMHGG
610 620 630 640 650
SNLWGDTKRP HGDTCGINQA LGSVAKIITD PDILKTCVPD MSDKRLEVVG
660 670 680 690 700
IEGVIEMGQI YTRLKSAGHR LAKCSEITFQ TTKTLPMQVD GEPWMQAPCT
710 720
IKITHKNQMP MLMGPAPSSS NFFGFWS
Length:727
Mass (Da):82,199
Last modified:October 1, 1996 - v1
Checksum:iB5A248ADD2F61C1D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S49760 mRNA Translation: AAB24434.1
PIRiA56879
RefSeqiNP_542965.1, NM_080787.1
UniGeneiRn.1840

Genome annotation databases

EnsembliENSRNOT00000036373; ENSRNOP00000038628; ENSRNOG00000022943
GeneIDi140866
KEGGirno:140866
UCSCiRGD:70904 rat

Similar proteinsi

Entry informationi

Entry nameiDGKA_RAT
AccessioniPrimary (citable) accession number: P51556
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health