Skip Header

Contribute Send feedback
Read comments (?) or add your own

P51556 (DGKA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diacylglycerol kinase alpha
Short name=DAG kinase alpha
EC=2.7.1.107
Alternative name(s):
80 kDa diacylglycerol kinase
Diglyceride kinase alpha
Short name=DGK-alpha
Gene names
Name:Dgka
Synonyms:Dagk, Dagk1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length727 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Upon cell stimulation converts the second messenger diacylglycerol into phosphatidate, initiating the resynthesis of phosphatidylinositols and attenuating protein kinase C activity.

Catalytic activity

ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Enzyme regulation

Stimulated by calcium and phosphatidylserine. Phosphorylated by protein kinase C By similarity.

Subunit structure

Monomer By similarity.

Tissue specificity

Lymphocytes and oligodendroglial cells.

Sequence similarities

Belongs to the eukaryotic diacylglycerol kinase family.

Contains 1 DAGKc domain.

Contains 2 EF-hand domains.

Contains 2 phorbol-ester/DAG-type zinc fingers.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 727727Diacylglycerol kinase alpha
PRO_0000218456

Regions

Domain108 – 14336EF-hand 1
Domain153 – 18836EF-hand 2
Domain365 – 498134DAGKc
Calcium binding121 – 132121 Probable
Calcium binding166 – 177122 Probable
Zinc finger203 – 25149Phorbol-ester/DAG-type 1
Zinc finger267 – 31751Phorbol-ester/DAG-type 2

Amino acid modifications

Modified residue221Phosphotyrosine By similarity
Modified residue4761N6-acetyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P51556 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B5A248ADD2F61C1D

FASTA72782,199
        10         20         30         40         50         60 
MAKDKGLISP EDFAQLQKYI DYSTKSVSDV LKVFEMNKYC QGDEIGYLGF EQFLKMYLEV 

        70         80         90        100        110        120 
EEVPHHLCWT LFWSFHSSQD LDEETESKAN VICLSDVYCY FTLLEGGSPE DKLEFTFKLY 

       130        140        150        160        170        180 
DMDRNGILDS TEVEKIILQM MRVAEYLDWD VSELRPILQE MMKEMDRDGS GCVSLAEWVR 

       190        200        210        220        230        240 
AGATTVPLLV LLGIDMTMKD DGHHIWRPKR FSRPVYCNLC ELSIGLGKQG LSCNLCKYIV 

       250        260        270        280        290        300 
HDHCAMKAQP CEVSTYAKSR KDIGVQPHVW VRGGCHSGRC DRCQKKIRTY HSLTGLHCVW 

       310        320        330        340        350        360 
CHLEIHDDCL QAVGPECDCG LLRDHILPPC SIYPRVLVSG QECKQKTTDV TSLCTPEAFR 

       370        380        390        400        410        420 
IEPVSNTHPL LVFINPKSGG KQGQSVLWKF QYILNPRQVF NLKDGPEPGL RFFKDVPQFR 

       430        440        450        460        470        480 
VLVCGGDGTV GWILETIDKA NFPIVPPVAV LPLGTGNDLA RCLRWGRGYE GENLRKILKD 

       490        500        510        520        530        540 
IEISKVVYLD RWLLEVIPQQ NGEKSDPVPS QIINNYFSIG VDASIAHRFH LMREKYPEKF 

       550        560        570        580        590        600 
NSRMKNKLWY LEFATSESIF STCKKLEESV TVEICGKLLD LSDLSLEGIA VLNIPSMHGG 

       610        620        630        640        650        660 
SNLWGDTKRP HGDTCGINQA LGSVAKIITD PDILKTCVPD MSDKRLEVVG IEGVIEMGQI 

       670        680        690        700        710        720 
YTRLKSAGHR LAKCSEITFQ TTKTLPMQVD GEPWMQAPCT IKITHKNQMP MLMGPAPSSS 


NFFGFWS

« Hide

References

[1]"Gene cloning, sequence, expression and in situ localization of 80 kDa diacylglycerol kinase specific to oligodendrocyte of rat brain."
Goto K., Watanabe M., Kondo H., Yuasa H., Sakane F., Kanoh H.
Brain Res. Mol. Brain Res. 16:75-87(1992) [PubMed: 1339302] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		S49760 mRNA. Translation: AAB24434.1.
IPIIPI00203295.
PIRA56879.
RefSeqNP_542965.1. NM_080787.1.
UniGeneRn.1840.

3D structure databases

ProteinModelPortalP51556.
SMRP51556. Positions 1-116.
ModBaseSearch...

Protein-protein interaction databases

STRINGP51556.

PTM databases

PhosphoSiteP51556.

Proteomic databases

PRIDEP51556.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID140866.
KEGGrno:140866.

Organism-specific databases

CTD1606.
RGD70904. Dgka.

Phylogenomic databases

eggNOGroNOG10067.
GeneTreeENSGT00600000084185.
HOVERGENHBG051345.
InParanoidP51556.
OrthoDBEOG4T782Q.

Gene expression databases

ArrayExpressP51556.
GenevestigatorP51556.
GermOnlineENSRNOG00000022943. Rattus norvegicus.

Family and domain databases

InterProIPR000756. Diacylglycerol_kin_accessory.
IPR001206. Diacylglycerol_kinase_cat_dom.
IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca-bd.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 1 hit.
KOK00901.
PfamPF00130. C1_1. 2 hits.
PF00609. DAGK_acc. 1 hit.
PF00781. DAGK_cat. 1 hit.
[Graphical view]
SMARTSM00109. C1. 2 hits.
SM00045. DAGKa. 1 hit.
SM00046. DAGKc. 1 hit.
SM00054. EFh. 2 hits.
[Graphical view]
PROSITEPS50146. DAGK. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 2 hits.
PS00479. ZF_DAG_PE_1. 2 hits.
PS50081. ZF_DAG_PE_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio620731.

Entry information

Entry nameDGKA_RAT
AccessionPrimary (citable) accession number: P51556
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 16, 2011
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents