Reviewed, UniProtKB/Swiss-Prot P51542 (CP7A1_RABIT)

Names and origin

Protein names

Recommended name:
    Cholesterol 7-alpha-monooxygenase
    EC=1.14.13.17
Alternative name(s):
    Cytochrome P450 7A1
    CYPVII
    Cholesterol 7-alpha-hydroxylase

Gene names

Name:	CYP7A1
Synonyms:	CYP7

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Cholesterol + NADPH + O2 = 7-alpha-hydroxycholesterol + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Lipid metabolism; bile acid biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	cholesterol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cholesterol 7-alpha-monooxygenase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Cholesterol 7-alpha-monooxygenase		PRO_0000051904
Sites
	Metal binding	441	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51542-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: FEF1247B151AA6B1	FASTA	501	58,090
	10 20 30 40 50 60 MITIFWIWGI CLSVCCCLWL ILGLRRRRMG EPPLEKGWIP YLGCALQFGA NPLDFLRANQ 70 80 90 100 110 120 RKYGHVFTCK LMGKYVHFIT NSLSYHKVLC HGKYFDWKKF HFTTSAKAFG HRSIDPRDGN 130 140 150 160 170 180 TTENINNTFN KTLQGDALIS LTDAMMENLQ LTLRRPEPKS RAWVTEGMYS FCYRVMFEAG 190 200 210 220 230 240 YLTLFGRELT RQDAQRAFIL NSLEDFKQFD KVFPALVAGL PIHIFMTAHN AREKLAEGLK 250 260 270 280 290 300 HDNLRTRDHI SELIRLRMFL NDTLSTFDAM EKAKTHLAIL WASQANTIPA TFWSLFHMMR 310 320 330 340 350 360 SSEALKAATE EVNKALEDAD QQINFEGKPI HLNQTQLNDM PVLDSIIKES LRLSSASLNI 370 380 390 400 410 420 RTAKEDFTLH LEDGSYNIRK DDIIALYPQL MHLDPEIYPD PMTFKYDRYL DENRKTKTTF 430 440 450 460 470 480 YSKGLKLKYY YMPFGSGATI CPGRLFAIQE IKQFLILMLS YFELEFVDSH VKCPPLDQSR 490 500 AGLGILPPLN DIEFKYKFKH L

References

[1]

"Synchronous circadian rhythms of mRNA levels and activities of cholesterol 7 alpha-hydroxylase in the rabbit and rat." Kai M., Eto T., Kondo K., Setoguchi Y., Higashi S., Maeda Y., Setoguchi T. J. Lipid Res. 36:367-374(1995) [PubMed: 7751825] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L10754 mRNA. Translation: AAA74382.1.
PIR	I46701.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P51542.
Enzyme and pathway databases
BRENDA	1.14.13.17. 255.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP7A1_RABIT

Accession

Primary (citable) accession number: P51542

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)