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Protein

Mannan endo-1,4-beta-mannosidase

Gene

manA

Organism
Streptomyces lividans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

pH dependencei

Optimum pH is 6.8.

Temperature dependencei

Optimum temperature is 58 degrees Celsius.

GO - Molecular functioni

  1. cellulose binding Source: InterPro
  2. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiCBM10. Carbohydrate-Binding Module Family 10.
GH5. Glycoside Hydrolase Family 5.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannan endo-1,4-beta-mannosidase (EC:3.2.1.78)
Alternative name(s):
1,4-beta-D-mannan mannanohydrolase
Beta-mannanase
Gene namesi
Name:manA
OrganismiStreptomyces lividans
Taxonomic identifieri1916 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 35351 PublicationAdd
BLAST
Chaini36 – 383348Mannan endo-1,4-beta-mannosidasePRO_0000007900Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP51529.
SMRiP51529. Positions 37-336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini345 – 37531CBM10Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi336 – 3405Poly-Gly

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR002883. CBM10/Dockerin_dom.
IPR009031. CBM_fam10.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02013. CBM_10. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTiSM01064. CBM_10. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51529-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRNARSTLIT TAGMAFAVLG LLFALAGPSA GRAEAAAGGI HVSNGRVVEG
60 70 80 90 100
NGSAFVMRGV NHAYTWYPDR TGSIADIAAK GANTVRVVLS SGGRWTKTSA
110 120 130 140 150
SEVSALIGQC KANKVICVLE VHDTTGYGKD GATSLDQAGD YWVGVKSAAW
160 170 180 190 200
RAQEDYVVVN IGNEPFGNTN YAAWTDATKS AIGKLRGAGL GHALMVDAPN
210 220 230 240 250
WGQDWSGTMR SNAASVFASD PDRNTVFSIH MYGVYDTAAE VRDYLNAFVG
260 270 280 290 300
NGLPIVVGEF GDQHSDGNPD EDAIMATAQS LGVGYLGWSW SGNGGGVEYL
310 320 330 340 350
DMVNGFDPNS LTSWGNRILY GSNGIAATSR TATVYGGGGG STGGTAPNGY
360 370 380
PYCVNGGASD PDGDGWGWEN SRSCVVRGSA ADH
Length:383
Mass (Da):39,682
Last modified:May 30, 2000 - v2
Checksum:i5DB4B407C64E94C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92297 Genomic DNA. Translation: AAA26710.2.
PIRiS30386.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M92297 Genomic DNA. Translation: AAA26710.2.
PIRiS30386.

3D structure databases

ProteinModelPortaliP51529.
SMRiP51529. Positions 37-336.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiCBM10. Carbohydrate-Binding Module Family 10.
GH5. Glycoside Hydrolase Family 5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR002883. CBM10/Dockerin_dom.
IPR009031. CBM_fam10.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF02013. CBM_10. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTiSM01064. CBM_10. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Beta-mannanase of Streptomyces lividans 66: cloning and DNA sequence of the manA gene and characterization of the enzyme."
    Arcand N., Kluepfel D., Paradis F.W., Morosoli R., Shareck F.
    Biochem. J. 290:857-863(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-42.
    Strain: 66 / 1326.
  2. Shareck F.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.

Entry informationi

Entry nameiMANA_STRLI
AccessioniPrimary (citable) accession number: P51529
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: May 30, 2000
Last modified: October 1, 2014
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.