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P51529

- MANA_STRLI

UniProt

P51529 - MANA_STRLI

Protein

Mannan endo-1,4-beta-mannosidase

Gene

manA

Organism
Streptomyces lividans
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (30 May 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans.

    pH dependencei

    Optimum pH is 6.8.

    Temperature dependencei

    Optimum temperature is 58 degrees Celsius.

    GO - Molecular functioni

    1. cellulose binding Source: InterPro
    2. mannan endo-1,4-beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiCBM10. Carbohydrate-Binding Module Family 10.
    GH5. Glycoside Hydrolase Family 5.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mannan endo-1,4-beta-mannosidase (EC:3.2.1.78)
    Alternative name(s):
    1,4-beta-D-mannan mannanohydrolase
    Beta-mannanase
    Gene namesi
    Name:manA
    OrganismiStreptomyces lividans
    Taxonomic identifieri1916 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 35351 PublicationAdd
    BLAST
    Chaini36 – 383348Mannan endo-1,4-beta-mannosidasePRO_0000007900Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    3D structure databases

    ProteinModelPortaliP51529.
    SMRiP51529. Positions 37-336.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini345 – 37531CBM10Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi336 – 3405Poly-Gly

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR002883. CBM10/Dockerin_dom.
    IPR009031. CBM_fam10.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF02013. CBM_10. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view]
    SMARTiSM01064. CBM_10. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P51529-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRNARSTLIT TAGMAFAVLG LLFALAGPSA GRAEAAAGGI HVSNGRVVEG    50
    NGSAFVMRGV NHAYTWYPDR TGSIADIAAK GANTVRVVLS SGGRWTKTSA 100
    SEVSALIGQC KANKVICVLE VHDTTGYGKD GATSLDQAGD YWVGVKSAAW 150
    RAQEDYVVVN IGNEPFGNTN YAAWTDATKS AIGKLRGAGL GHALMVDAPN 200
    WGQDWSGTMR SNAASVFASD PDRNTVFSIH MYGVYDTAAE VRDYLNAFVG 250
    NGLPIVVGEF GDQHSDGNPD EDAIMATAQS LGVGYLGWSW SGNGGGVEYL 300
    DMVNGFDPNS LTSWGNRILY GSNGIAATSR TATVYGGGGG STGGTAPNGY 350
    PYCVNGGASD PDGDGWGWEN SRSCVVRGSA ADH 383
    Length:383
    Mass (Da):39,682
    Last modified:May 30, 2000 - v2
    Checksum:i5DB4B407C64E94C3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92297 Genomic DNA. Translation: AAA26710.2.
    PIRiS30386.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M92297 Genomic DNA. Translation: AAA26710.2 .
    PIRi S30386.

    3D structure databases

    ProteinModelPortali P51529.
    SMRi P51529. Positions 37-336.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi CBM10. Carbohydrate-Binding Module Family 10.
    GH5. Glycoside Hydrolase Family 5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR002883. CBM10/Dockerin_dom.
    IPR009031. CBM_fam10.
    IPR001547. Glyco_hydro_5.
    IPR018087. Glyco_hydro_5_CS.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF02013. CBM_10. 1 hit.
    PF00150. Cellulase. 1 hit.
    [Graphical view ]
    SMARTi SM01064. CBM_10. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Beta-mannanase of Streptomyces lividans 66: cloning and DNA sequence of the manA gene and characterization of the enzyme."
      Arcand N., Kluepfel D., Paradis F.W., Morosoli R., Shareck F.
      Biochem. J. 290:857-863(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 36-42.
      Strain: 66 / 1326.
    2. Shareck F.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.

    Entry informationi

    Entry nameiMANA_STRLI
    AccessioniPrimary (citable) accession number: P51529
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: May 30, 2000
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3