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Protein

Phospholipase A1

Gene
N/A
Organism
Vespula maculifrons (Eastern yellow jacket) (Wasp)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities.By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei137 – 1371NucleophileBy similarity
Active sitei165 – 1651Charge relay systemPROSITE-ProRule annotation
Active sitei229 – 2291Charge relay systemPROSITE-ProRule annotation

GO - Molecular functioni

  1. 1-acyl-2-lysophosphatidylserine acylhydrolase activity Source: UniProtKB-EC
  2. phosphatidylcholine 1-acylhydrolase activity Source: UniProtKB-EC
  3. phosphatidylserine 1-acylhydrolase activity Source: UniProtKB-EC
  4. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A1 (EC:3.1.1.32, EC:3.1.1.4)
Alternative name(s):
Allergen Ves m I
Allergen: Ves m 1
OrganismiVespula maculifrons (Eastern yellow jacket) (Wasp)
Taxonomic identifieri7453 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataVespoideaVespidaeVespinaeVespula

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei3514. Ves m 1.0101.
661. Ves m 1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Phospholipase A1PRO_0000090378Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi4 ↔ 244By similarity
Disulfide bondi87 ↔ 227By similarity
Disulfide bondi176 ↔ 219By similarity
Disulfide bondi181 ↔ 261By similarity
Disulfide bondi239 ↔ 245By similarity
Disulfide bondi268 ↔ 293By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP51528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002334. Allerg_PlipaseA1.
IPR013818. Lipase_N.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
[Graphical view]
PRINTSiPR00825. DOLALLERGEN.
PR00821. TAGLIPASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
GPKCPFNSDT VSIIIETREN RNRDLYTLQT LQNHPEFKKK TITRPVVFIT
60 70 80 90 100
HGFTSSASEK NFINLAKALV DKDNYMVISI DWQTAACTNE YPGLKYAYYP
110 120 130 140 150
TAASNTRLVG QYIATITQKL VKDYKISMAN IRLIGHSLGA HVSGFAGKRV
160 170 180 190 200
QELKLGKYSE IIGLDPARPS FDSNHCSERL CETDAEYVQI IHTSNYLGTE
210 220 230 240 250
KILGTVDFYM NNGKNNPGCG RFFSEVCSHT RAVIYMAECI KHECCLIGIP
260 270 280 290 300
RSKSSQPISR CTKQECVCVG LNAKKYPSRG SFYVPVESTA PFCNNKGKII
Length:300
Mass (Da):33,540
Last modified:September 30, 1996 - v1
Checksum:i8EE2DE20BD69CCF6
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti97 – 971A → M.
Natural varianti191 – 1911I → P.
Natural varianti202 – 2021I → L.

Sequence databases

PIRiA44564.

Cross-referencesi

Sequence databases

PIRiA44564.

3D structure databases

ProteinModelPortaliP51528.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei3514. Ves m 1.0101.
661. Ves m 1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002334. Allerg_PlipaseA1.
IPR013818. Lipase_N.
IPR000734. TAG_lipase.
[Graphical view]
PANTHERiPTHR11610. PTHR11610. 1 hit.
PfamiPF00151. Lipase. 1 hit.
[Graphical view]
PRINTSiPR00825. DOLALLERGEN.
PR00821. TAGLIPASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Allergens in hymenoptera venom. XXVI: the complete amino acid sequences of two vespid venom phospholipases."
    Hoffman D.R.
    Int. Arch. Allergy Immunol. 104:184-190(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Tissue: Venom.

Entry informationi

Entry nameiPA1_VESMC
AccessioniPrimary (citable) accession number: P51528
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: September 30, 1996
Last modified: January 6, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.