P51528 (PA1_VESMC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phospholipase A1 EC=3.1.1.32 EC=3.1.1.4 Alternative name(s): Allergen Ves m I Allergen=Ves m 1
Organism	Vespula maculifrons (Eastern yellow jacket) (Wasp)
Taxonomic identifier	7453 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Aculeata › Vespoidea › Vespidae › Vespinae › Vespula

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolysis of phosphatidylcholine with phospholipase A2 (EC 3.1.1.4) and phospholipase A1 (EC 3.1.1.32) activities By similarity.
Catalytic activity	Phosphatidylcholine + H2O = 2-acylglycerophosphocholine + a carboxylate. Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.
Subunit structure	Monomer.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	Contains six disulfide bonds By similarity.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Secreted
Disease	Allergen
Molecular function	Hydrolase
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-acyl-2-lysophosphatidylserine acylhydrolase activity Inferred from electronic annotation. Source: EC phosphatidylcholine 1-acylhydrolase activity Inferred from electronic annotation. Source: EC phosphatidylserine 1-acylhydrolase activity Inferred from electronic annotation. Source: EC phospholipase A2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Phospholipase A1		PRO_0000090378
Sites
	Active site	137	1	Nucleophile By similarity
	Active site	165	1	Charge relay system By similarity
	Active site	229	1	Charge relay system By similarity
Natural variations
	Natural variant	97	1	A → M.
	Natural variant	191	1	I → P.
	Natural variant	202	1	I → L.

Sequences

	Sequence		Length	Mass (Da)
	P51528 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 8EE2DE20BD69CCF6	FASTA	300	33,540
	10 20 30 40 50 60 GPKCPFNSDT VSIIIETREN RNRDLYTLQT LQNHPEFKKK TITRPVVFIT HGFTSSASEK 70 80 90 100 110 120 NFINLAKALV DKDNYMVISI DWQTAACTNE YPGLKYAYYP TAASNTRLVG QYIATITQKL 130 140 150 160 170 180 VKDYKISMAN IRLIGHSLGA HVSGFAGKRV QELKLGKYSE IIGLDPARPS FDSNHCSERL 190 200 210 220 230 240 CETDAEYVQI IHTSNYLGTE KILGTVDFYM NNGKNNPGCG RFFSEVCSHT RAVIYMAECI 250 260 270 280 290 300 KHECCLIGIP RSKSSQPISR CTKQECVCVG LNAKKYPSRG SFYVPVESTA PFCNNKGKII

References

[1]	"Allergens in hymenoptera venom. XXVI: the complete amino acid sequences of two vespid venom phospholipases." Hoffman D.R. Int. Arch. Allergy Immunol. 104:184-190(1994) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Venom.

Cross-references

Sequence databases
PIR	A44564.
3D structure databases
ProteinModelPortal	P51528.
ModBase	Search...
Protein family/group databases
Allergome	3514. Ves m 1.0101. 661. Ves m 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002334. Dol/Ves_allerg. IPR000734. Lipase. IPR013818. Lipase_N. [Graphical view]
PANTHER	PTHR11610. PTHR11610. 1 hit.
Pfam	PF00151. Lipase. 1 hit. [Graphical view]
PRINTS	PR00825. DOLALLERGEN. PR00821. TAGLIPASE.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PA1_VESMC

Accession

Primary (citable) accession number: P51528

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)