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Protein

Transcription factor 12

Gene

Tcf12

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Activates transcription by binding to the E box (5'-CANNTG-3') (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription factor 12
Short name:
TCF-12
Alternative name(s):
DNA-binding protein HTF4
E-box-binding protein
Salivary-specific cAMP response element-binding protein alpha
Short name:
SCBP-alpha
Transcription factor HTF-4
Gene namesi
Name:Tcf12
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 8

Organism-specific databases

RGDi3829. Tcf12.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 707707Transcription factor 12PRO_0000127232Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei47 – 471PhosphoserineBy similarity
Modified residuei67 – 671PhosphoserineCombined sources
Modified residuei79 – 791PhosphoserineCombined sources
Modified residuei98 – 981PhosphoserineBy similarity
Modified residuei116 – 1161PhosphoserineBy similarity
Modified residuei124 – 1241PhosphoserineBy similarity
Modified residuei313 – 3131PhosphothreonineBy similarity
Modified residuei333 – 3331PhosphoserineBy similarity
Cross-linki544 – 544Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei565 – 5651PhosphoserineBy similarity
Cross-linki575 – 575Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei582 – 5821PhosphothreonineBy similarity
Modified residuei583 – 5831PhosphoserineBy similarity
Modified residuei584 – 5841PhosphoserineBy similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP51514.

PTM databases

iPTMnetiP51514.
PhosphoSiteiP51514.

Expressioni

Tissue specificityi

Isoform gamma is highly expressed in lung, kidney, spleen, and is expressed at reduced levels in heart, muscle, liver, pituitary, brain and the trigeminal ganglion. The expression of isoform alpha predominates over isoform gamma in the pituitary and the brain.

Gene expression databases

ExpressionAtlasiP51514. differential.
GenevisibleiP51514. RN.

Interactioni

Subunit structurei

Efficient DNA binding requires dimerization with another bHLH protein. Forms homo- or heterooligomers with myogenin, E12 and ITF2 proteins. Interacts with PTF1. Interacts with RUNX1T1. Interacts with NEUROD2 (By similarity). Interacts with BHLHA9.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023634.

Structurei

3D structure databases

ProteinModelPortaliP51514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini602 – 65554bHLHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni119 – 14022Leucine-zipperAdd
BLAST
Regioni657 – 68024Class A specific domainAdd
BLAST

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3910. Eukaryota.
ENOG410XYUA. LUCA.
GeneTreeiENSGT00510000046438.
HOGENOMiHOG000234180.
HOVERGENiHBG003854.
InParanoidiP51514.
KOiK15603.
PhylomeDBiP51514.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform Alpha (identifier: P51514-1) [UniParc]FASTAAdd to basket

Also known as: Beta

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNPQQQRMAA IGTDKELSDL LDFSAMFSPP VNSGKTRPTT LGSSQFSGSG
60 70 80 90 100
MDERGGTTSW GTSGQPSPSY DSSRGFTDSP HYSDHLNDSR LGTHEGLSPT
110 120 130 140 150
PFMNSNLIGK TSERGSFSLY SRDSGLSGCQ SSLLRQDLGL GSPAQLSSSG
160 170 180 190 200
KPGTPYYSFS ATSSRRRPLH DSVALDPLQA KKVRKVPPGL PSSVYAPSPN
210 220 230 240 250
SDDFNRESPS YPSPKPPTSM FASTFFMQDG THSSSDLWSS SNGMSQPGFG
260 270 280 290 300
GILGTSTSHM SQSSSYGSLH SHDRLSYPPH SVSPTDINTS LPPMSSFHRG
310 320 330 340 350
STSSSPYVAA SHTPPINGSD SILGTRGNAA GSSQTGDALG KALASIYSPD
360 370 380 390 400
HTSSSFPSNP STPVGSPSPL TAGTSQWPRA GGQAPSSPSY ENSLHSLKNR
410 420 430 440 450
VEQQLHEHLQ DAMSFLKDVC EQSRMEDRLD RLDDAIHVLR NHAVGPSTSL
460 470 480 490 500
PTSHSDIHSL LGPSHNAPIG NLNSNYGGSS LVTNSRSASM VGTHREDSVS
510 520 530 540 550
LNGNHSVLSS TVAASNTELN HKTPESFRGG VQNQSGSVVP TEIKTENKEK
560 570 580 590 600
DENLHEPPSS DDMKSDDESS QKDIKVSSRG RTSSTNEDED LNPEQKIERE
610 620 630 640 650
KERRMANNAR ERLRVRDINE AFKELGRMCQ LHLKSEKPQT KLLILHQAVA
660 670 680 690 700
VILSLEQQVR ERNLNPKAAC LKRREEEKVS AASAEPPTTL PGTHPGLSET

TNPMGHL
Length:707
Mass (Da):75,896
Last modified:October 1, 1996 - v1
Checksum:iCC4AC60294601E2B
GO
Isoform Beta (identifier: P51514-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     422-422: Missing.

Show »
Length:706
Mass (Da):75,768
Checksum:iA99DEED24928875E
GO
Isoform Gamma (identifier: P51514-3) [UniParc]FASTAAdd to basket

Also known as: Alpha

The sequence of this isoform differs from the canonical sequence as follows:
     398-422: Missing.

Show »
Length:682
Mass (Da):72,888
Checksum:i3F82ADCDD66FA050
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti80 – 801P → A in AAB25128 (PubMed:8422988).Curated
Sequence conflicti80 – 801P → A in AAB25129 (PubMed:8422988).Curated
Sequence conflicti124 – 1241S → C in AAB25128 (PubMed:8422988).Curated
Sequence conflicti124 – 1241S → C in AAB25129 (PubMed:8422988).Curated
Sequence conflicti159 – 1591F → S in AAB25128 (PubMed:8422988).Curated
Sequence conflicti159 – 1591F → S in AAB25129 (PubMed:8422988).Curated
Sequence conflicti541 – 5411T → N in AAB25128 (PubMed:8422988).Curated
Sequence conflicti541 – 5411T → N in AAB25129 (PubMed:8422988).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei398 – 42225Missing in isoform Gamma. CuratedVSP_002106Add
BLAST
Alternative sequencei422 – 4221Missing in isoform Beta. CuratedVSP_002107

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09656 mRNA. Translation: AAA42115.1.
S53913 mRNA. Translation: AAB25128.2.
S53920 mRNA. Translation: AAB25129.2. Sequence problems.
S53922 Genomic DNA. No translation available.
S53921 Genomic DNA. No translation available.
PIRiA46691.
RefSeqiNP_037308.1. NM_013176.2. [P51514-1]
XP_006243408.1. XM_006243346.1. [P51514-2]
XP_006243411.1. XM_006243349.1. [P51514-3]
UniGeneiRn.165133.
Rn.9916.

Genome annotation databases

EnsembliENSRNOT00000081185; ENSRNOP00000074384; ENSRNOG00000057754. [P51514-1]
GeneIDi25720.
KEGGirno:25720.
UCSCiRGD:3829. rat. [P51514-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L09656 mRNA. Translation: AAA42115.1.
S53913 mRNA. Translation: AAB25128.2.
S53920 mRNA. Translation: AAB25129.2. Sequence problems.
S53922 Genomic DNA. No translation available.
S53921 Genomic DNA. No translation available.
PIRiA46691.
RefSeqiNP_037308.1. NM_013176.2. [P51514-1]
XP_006243408.1. XM_006243346.1. [P51514-2]
XP_006243411.1. XM_006243349.1. [P51514-3]
UniGeneiRn.165133.
Rn.9916.

3D structure databases

ProteinModelPortaliP51514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023634.

PTM databases

iPTMnetiP51514.
PhosphoSiteiP51514.

Proteomic databases

PaxDbiP51514.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000081185; ENSRNOP00000074384; ENSRNOG00000057754. [P51514-1]
GeneIDi25720.
KEGGirno:25720.
UCSCiRGD:3829. rat. [P51514-1]

Organism-specific databases

CTDi6938.
RGDi3829. Tcf12.

Phylogenomic databases

eggNOGiKOG3910. Eukaryota.
ENOG410XYUA. LUCA.
GeneTreeiENSGT00510000046438.
HOGENOMiHOG000234180.
HOVERGENiHBG003854.
InParanoidiP51514.
KOiK15603.
PhylomeDBiP51514.

Miscellaneous databases

PROiP51514.

Gene expression databases

ExpressionAtlasiP51514. differential.
GenevisibleiP51514. RN.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The helix-loop-helix proteins (salivary-specific cAMP response element-binding proteins) can modulate cAMP-inducible RP4 gene expression in salivary cells."
    Lin H.H., Li W.-Y., Ann D.K.
    J. Biol. Chem. 268:10214-10220(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Salivary gland.
  2. "Tissue-specific RNA splicing generates an ankyrin-like domain that affects the dimerization and DNA-binding properties of a bHLH protein."
    Klein E.S., Simmons D.M., Swanson L.W., Rosenfeld M.G.
    Genes Dev. 7:55-71(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 80-707.
  3. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-67 AND SER-79, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHTF4_RAT
AccessioniPrimary (citable) accession number: P51514
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 6, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.