P51513 (NOVA1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-binding protein Nova-1

Alternative name(s):
Neuro-oncological ventral antigen 1
Onconeural ventral antigen 1
Paraneoplastic Ri antigen
Ventral neuron-specific protein 1

Gene names

Name:

NOVA1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May regulate RNA splicing or metabolism in a specific subset of developing neurons.
Subunit structure	Interacts with PTBP2; the interaction is direct By similarity.
Subcellular location	Nucleus.
Tissue specificity	Brain.
Sequence similarities	Contains 3 KH domains.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA splicing Traceable author statement Ref.1. Source: ProtInc locomotory behavior Traceable author statement PubMed 8558240. Source: ProtInc mRNA splicing, via spliceosome Inferred from electronic annotation. Source: Compara synaptic transmission Traceable author statement PubMed 8558240. Source: ProtInc
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Traceable author statement PubMed 8558240. Source: ProtInc mRNA binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P51513-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P51513-2)

Also known as: Tumor;

The sequence of this isoform differs from the canonical sequence as follows:
     88-90: Missing.
     177-184: VKIIVPNS → KHNISWIS
     185-510: Missing.

Isoform 3 (identifier: P51513-3)

The sequence of this isoform differs from the canonical sequence as follows:
     153-176: Missing.

Isoform 4 (identifier: P51513-4)

The sequence of this isoform differs from the canonical sequence as follows:
     88-90: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 510

510

RNA-binding protein Nova-1

PRO_0000050116

Regions

Domain

49 – 119

71

KH 1

Domain

174 – 240

67

KH 2

Domain

424 – 491

68

KH 3

Motif

27 – 43

17

Bipartite nuclear localization signal Potential

Compositional bias

276 – 412

137

Ala-rich

Amino acid modifications

Modified residue

157

1

Phosphoserine By similarity

Natural variations

Alternative sequence

88 – 90

3

Missing in isoform 2 and isoform 4.

VSP_002841

Alternative sequence

153 – 176

24

Missing in isoform 3.

VSP_002842

Alternative sequence

177 – 184

8

VKIIVPNS → KHNISWIS in isoform 2.

VSP_002843

Alternative sequence

185 – 510

326

Missing in isoform 2.

VSP_002844

Secondary structure

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510

Helix Strand Turn

Details...

Beta strand

50 – 57

8

Helix

58 – 65

8

Helix

67 – 69

3

Helix

70 – 79

10

Beta strand

98 – 107

10

Helix

109 – 123

15

Helix

173 – 175

3

Beta strand

176 – 182

7

Helix

183 – 190

8

Helix

192 – 194

3

Helix

195 – 203

9

Beta strand

207 – 210

4

Beta strand

221 – 229

9

Helix

230 – 245

16

Beta strand

425 – 432

8

Turn

433 – 436

4

Helix

437 – 440

4

Helix

442 – 444

3

Helix

445 – 454

10

Beta strand

457 – 460

4

Beta strand

472 – 479

8

Helix

481 – 494

14

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7B9BA8B67CAB6984	FASTA	510	52,056
	10 20 30 40 50 60 MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA 70 80 90 100 110 120 AGSIIGKGGQ TIVQLQKETG ATIKLSKLSK SKDFYPGTTE RVCLIQGTVE ALNAVHGFIA 130 140 150 160 170 180 EKIREMPQNV AKTEPVSILQ PQTTVNPDRI KQTLPSSPTT TKSSPSDPMT TSRANQVKII 190 200 210 220 230 240 VPNSTAGLII GKGGATVKAV MEQSGAWVQL SQKPDGINLQ ERVVTVSGEP EQNRKAVELI 250 260 270 280 290 300 IQKIQEDPQS GSCLNISYAN VTGPVANSNP TGSPYANTAE VLPTAAAAAG LLGHANLAGV 310 320 330 340 350 360 AAFPAVLSGF TGNDLVAITS ALNTLASYGY NLNTLGLGLS QAAATGALAA AAASANPAAA 370 380 390 400 410 420 AANLLATYAS EASASGSTAG GTAGTFALGS LAAATAATNG YFGAASPLAA SAILGTEKST 430 440 450 460 470 480 DGSKDVVEIA VPENLVGAIL GKGGKTLVEY QELTGARIQI SKKGEFVPGT RNRKVTITGT 490 500 510 PAATQAAQYL ITQRITYEQG VRAANPQKVG
	Isoform 2 (Tumor) [UniParc]. Checksum: 23EF1C6F5541D52D	FASTA	181	19,491
	10 20 30 40 50 60 MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA 70 80 90 100 110 120 AGSIIGKGGQ TIVQLQKETG ATIKLSKSKD FYPGTTERVC LIQGTVEALN AVHGFIAEKI 130 140 150 160 170 180 REMPQNVAKT EPVSILQPQT TVNPDRIKQT LPSSPTTTKS SPSDPMTTSR ANQKHNISWI S
	Isoform 3 [UniParc]. Checksum: 7F9A8331C469877E	FASTA	486	49,581
	10 20 30 40 50 60 MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA 70 80 90 100 110 120 AGSIIGKGGQ TIVQLQKETG ATIKLSKLSK SKDFYPGTTE RVCLIQGTVE ALNAVHGFIA 130 140 150 160 170 180 EKIREMPQNV AKTEPVSILQ PQTTVNPDRI KQVKIIVPNS TAGLIIGKGG ATVKAVMEQS 190 200 210 220 230 240 GAWVQLSQKP DGINLQERVV TVSGEPEQNR KAVELIIQKI QEDPQSGSCL NISYANVTGP 250 260 270 280 290 300 VANSNPTGSP YANTAEVLPT AAAAAGLLGH ANLAGVAAFP AVLSGFTGND LVAITSALNT 310 320 330 340 350 360 LASYGYNLNT LGLGLSQAAA TGALAAAAAS ANPAAAAANL LATYASEASA SGSTAGGTAG 370 380 390 400 410 420 TFALGSLAAA TAATNGYFGA ASPLAASAIL GTEKSTDGSK DVVEIAVPEN LVGAILGKGG 430 440 450 460 470 480 KTLVEYQELT GARIQISKKG EFVPGTRNRK VTITGTPAAT QAAQYLITQR ITYEQGVRAA NPQKVG
	Isoform 4 [UniParc]. Checksum: DBF455477988CDCA	FASTA	507	51,727
	10 20 30 40 50 60 MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA 70 80 90 100 110 120 AGSIIGKGGQ TIVQLQKETG ATIKLSKSKD FYPGTTERVC LIQGTVEALN AVHGFIAEKI 130 140 150 160 170 180 REMPQNVAKT EPVSILQPQT TVNPDRIKQT LPSSPTTTKS SPSDPMTTSR ANQVKIIVPN 190 200 210 220 230 240 STAGLIIGKG GATVKAVMEQ SGAWVQLSQK PDGINLQERV VTVSGEPEQN RKAVELIIQK 250 260 270 280 290 300 IQEDPQSGSC LNISYANVTG PVANSNPTGS PYANTAEVLP TAAAAAGLLG HANLAGVAAF 310 320 330 340 350 360 PAVLSGFTGN DLVAITSALN TLASYGYNLN TLGLGLSQAA ATGALAAAAA SANPAAAAAN 370 380 390 400 410 420 LLATYASEAS ASGSTAGGTA GTFALGSLAA ATAATNGYFG AASPLAASAI LGTEKSTDGS 430 440 450 460 470 480 KDVVEIAVPE NLVGAILGKG GKTLVEYQEL TGARIQISKK GEFVPGTRNR KVTITGTPAA 490 500 TQAAQYLITQ RITYEQGVRA ANPQKVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nova, the paraneoplastic Ri antigen, is homologous to an RNA-binding protein and is specifically expressed in the developing motor system." Buckanovich R.J., Posner J.B., Darnell R.B. Neuron 11:657-672(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Cerebellum and Hippocampus.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2 AND 4). Tissue: Amygdala.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Brain.
[5]	Dmitrenko V.V., Garifulin O.M., Shostak K.A., Smikodub A.I., Kavsan V.M. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-34. Tissue: Fetal brain.
[6]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"Crystal structures of Nova-1 and Nova-2 K-homology RNA-binding domains." Lewis H.A., Chen H., Edo C., Buckanovich R.J., Yang Y.Y.-L., Musunuru K., Zhong R., Darnell R.B., Burley S.K. Structure 7:191-203(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 423-495.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U04840 mRNA. Translation: AAA16022.1.
AK289639 mRNA. Translation: BAF82328.1.
AK291022 mRNA. Translation: BAF83711.1.
CH471078 Genomic DNA. Translation: EAW65991.1.
CH471078 Genomic DNA. Translation: EAW65993.1.
CH471078 Genomic DNA. Translation: EAW65995.1.
BC075038 mRNA. Translation: AAH75038.1.
BC075039 mRNA. Translation: AAH75039.1.
Z70771 mRNA. Translation: CAA94810.1.

IPI

IPI00019244.
IPI00221187.
IPI00221188.
IPI00816603.

PIR

I38489.

RefSeq

NP_002506.2. NM_002515.2.
NP_006480.2. NM_006489.2.
NP_006482.1. NM_006491.2.

UniGene

Hs.31588.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DT4	X-ray	2.60	A	424-495	[»]
2ANN	X-ray	2.30	A	49-249	[»]
2ANR	X-ray	1.94	A	49-249	[»]

ProteinModelPortal

P51513.

ModBase

Search...

Protein-protein interaction databases

IntAct

P51513. 2 interactions.

MINT

MINT-1381951.

STRING

9606.ENSP00000267422.

PTM databases

PhosphoSite

P51513.

Polymorphism databases

DMDM

1709303.

Proteomic databases

PaxDb

P51513.

PRIDE

P51513.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000344429; ENSP00000342387; ENSG00000139910.
ENST00000539517; ENSP00000438875; ENSG00000139910.

GeneID

4857.

KEGG

hsa:4857.

UCSC

uc001wpy.3. human.
uc001wqa.3. human.
uc001wqb.3. human.

Organism-specific databases

CTD

4857.

GeneCards

GC14M026915.

H-InvDB

HIX0037755.

HGNC

HGNC:7886. NOVA1.

HPA

HPA004155.

MIM

602157. gene.

neXtProt

NX_P51513.

PharmGKB

PA31688.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG258094.

HOGENOM

HOG000007833.

HOVERGEN

HBG082048.

InParanoid

P51513.

KO

K14944.

OMA

FRQNNTA.

Gene expression databases

ArrayExpress

P51513.

Bgee

P51513.

CleanEx

HS_NOVA1.

Genevestigator

P51513.

GermOnline

ENSG00000139910. Homo sapiens.

Family and domain databases

InterPro

IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
[Graphical view]

Pfam

PF00013. KH_1. 3 hits.
[Graphical view]

SMART

SM00322. KH. 3 hits.
[Graphical view]

PROSITE

PS50084. KH_TYPE_1. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

NOVA1. human.

EvolutionaryTrace

P51513.

GenomeRNAi

4857.

NextBio

18710.

SOURCE

Search...

Entry information

Entry name

NOVA1_HUMAN

Accession

Primary (citable) accession number: P51513
Secondary accession number(s): A8K0S4 , A8K4Q7, D3DS82, Q6B004

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.