Reviewed, UniProtKB/Swiss-Prot P51513 (NOVA1_HUMAN)

Names and origin

Protein names

Recommended name:
    RNA-binding protein Nova-1
Alternative name(s):
    Neuro-oncological ventral antigen 1
    Onconeural ventral antigen 1
    Paraneoplastic Ri antigen
    Ventral neuron-specific protein 1

Gene names

Name:

NOVA1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May regulate RNA splicing or metabolism in a specific subset of developing neurons.
Subunit structure	Interacts with PTBP2 By similarity.
Subcellular location	Nucleus.
Tissue specificity	Brain.
Involvement in disease	Autoantigen in the paraneoplastic opsoclonus myoclonus ataxia (POMA), a paraneoplastic neurological syndrome/disorder (PNS/D) associated with breast cancer, fallopian cancer, and SCLCa and characterized primarily by loss of inhibitory control of motor neurons in the spinal cord and brainstem. Recognized by the IgG autoantibody ANNA-2 (also called anti-Ri).
Sequence similarities	Contains 3 KH domains.

Ontologies

Keywords
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	RNA-binding
Technical term	3D-structure
Gene Ontology (GO)
Biological process	locomotory behavior Traceable author statement. Source: ProtInc synaptic transmission Traceable author statement. Source: ProtInc
Cellular component	nucleolus Inferred from direct assay. Source: HPA
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P51513-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P51513-2)

Also known as: Tumor;

The sequence of this isoform differs from the canonical sequence as follows:
     88-90: Missing.
     177-184: VKIIVPNS → KHNISWIS
     185-510: Missing.

Isoform 3 (identifier: P51513-3)

The sequence of this isoform differs from the canonical sequence as follows:
     153-176: Missing.

Isoform 4 (identifier: P51513-4)

The sequence of this isoform differs from the canonical sequence as follows:
     88-90: Missing.

Notes: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 510

510

RNA-binding protein Nova-1

PRO_0000050116

Regions

Domain

49 – 119

71

KH 1

Domain

174 – 240

67

KH 2

Domain

424 – 491

68

KH 3

Motif

27 – 43

17

Bipartite nuclear localization signal Potential

Compositional bias

276 – 412

137

Ala-rich

Natural variations

Alternative sequence

88 – 90

3

Missing in isoform 2 and isoform 4.

VSP_002841

Alternative sequence

153 – 176

24

Missing in isoform 3.

VSP_002842

Alternative sequence

177 – 184

8

VKIIVPNS → KHNISWIS in isoform 2.

VSP_002843

Alternative sequence

185 – 510

326

Missing in isoform 2.

VSP_002844

Secondary structure

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510

Helix Strand Turn

Details...

Beta strand

50 – 57

8

Helix

58 – 65

8

Helix

67 – 69

3

Helix

70 – 79

10

Beta strand

82 – 88

7

Beta strand

98 – 107

10

Helix

109 – 123

15

Helix

148 – 175

28

Beta strand

176 – 182

7

Helix

183 – 190

8

Helix

192 – 194

3

Helix

195 – 204

10

Beta strand

207 – 210

4

Beta strand

221 – 228

8

Helix

230 – 246

17

Beta strand

425 – 432

8

Turn

433 – 436

4

Helix

437 – 440

4

Helix

442 – 444

3

Helix

445 – 454

10

Beta strand

457 – 460

4

Beta strand

472 – 479

8

Helix

481 – 494

14

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7B9BA8B67CAB6984	FASTA	510	52,056
	10 20 30 40 50 60 MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA 70 80 90 100 110 120 AGSIIGKGGQ TIVQLQKETG ATIKLSKLSK SKDFYPGTTE RVCLIQGTVE ALNAVHGFIA 130 140 150 160 170 180 EKIREMPQNV AKTEPVSILQ PQTTVNPDRI KQTLPSSPTT TKSSPSDPMT TSRANQVKII 190 200 210 220 230 240 VPNSTAGLII GKGGATVKAV MEQSGAWVQL SQKPDGINLQ ERVVTVSGEP EQNRKAVELI 250 260 270 280 290 300 IQKIQEDPQS GSCLNISYAN VTGPVANSNP TGSPYANTAE VLPTAAAAAG LLGHANLAGV 310 320 330 340 350 360 AAFPAVLSGF TGNDLVAITS ALNTLASYGY NLNTLGLGLS QAAATGALAA AAASANPAAA 370 380 390 400 410 420 AANLLATYAS EASASGSTAG GTAGTFALGS LAAATAATNG YFGAASPLAA SAILGTEKST 430 440 450 460 470 480 DGSKDVVEIA VPENLVGAIL GKGGKTLVEY QELTGARIQI SKKGEFVPGT RNRKVTITGT 490 500 510 PAATQAAQYL ITQRITYEQG VRAANPQKVG
	Isoform 2 (Tumor) [UniParc]. Checksum: 23EF1C6F5541D52D		181	19,491
	10 20 30 40 50 60 MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA 70 80 90 100 110 120 AGSIIGKGGQ TIVQLQKETG ATIKLSKSKD FYPGTTERVC LIQGTVEALN AVHGFIAEKI 130 140 150 160 170 180 REMPQNVAKT EPVSILQPQT TVNPDRIKQT LPSSPTTTKS SPSDPMTTSR ANQKHNISWI S
	Isoform 3 [UniParc]. Checksum: 7F9A8331C469877E		486	49,581
	10 20 30 40 50 60 MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA 70 80 90 100 110 120 AGSIIGKGGQ TIVQLQKETG ATIKLSKLSK SKDFYPGTTE RVCLIQGTVE ALNAVHGFIA 130 140 150 160 170 180 EKIREMPQNV AKTEPVSILQ PQTTVNPDRI KQVKIIVPNS TAGLIIGKGG ATVKAVMEQS 190 200 210 220 230 240 GAWVQLSQKP DGINLQERVV TVSGEPEQNR KAVELIIQKI QEDPQSGSCL NISYANVTGP 250 260 270 280 290 300 VANSNPTGSP YANTAEVLPT AAAAAGLLGH ANLAGVAAFP AVLSGFTGND LVAITSALNT 310 320 330 340 350 360 LASYGYNLNT LGLGLSQAAA TGALAAAAAS ANPAAAAANL LATYASEASA SGSTAGGTAG 370 380 390 400 410 420 TFALGSLAAA TAATNGYFGA ASPLAASAIL GTEKSTDGSK DVVEIAVPEN LVGAILGKGG 430 440 450 460 470 480 KTLVEYQELT GARIQISKKG EFVPGTRNRK VTITGTPAAT QAAQYLITQR ITYEQGVRAA NPQKVG
	Isoform 4 [UniParc]. Checksum: DBF455477988CDCA		507	51,727
	10 20 30 40 50 60 MMAAAPIQQN GTHTGVPIDL DPPDSRKRPL EAPPEAGSTK RTNTGEDGQY FLKVLIPSYA 70 80 90 100 110 120 AGSIIGKGGQ TIVQLQKETG ATIKLSKSKD FYPGTTERVC LIQGTVEALN AVHGFIAEKI 130 140 150 160 170 180 REMPQNVAKT EPVSILQPQT TVNPDRIKQT LPSSPTTTKS SPSDPMTTSR ANQVKIIVPN 190 200 210 220 230 240 STAGLIIGKG GATVKAVMEQ SGAWVQLSQK PDGINLQERV VTVSGEPEQN RKAVELIIQK 250 260 270 280 290 300 IQEDPQSGSC LNISYANVTG PVANSNPTGS PYANTAEVLP TAAAAAGLLG HANLAGVAAF 310 320 330 340 350 360 PAVLSGFTGN DLVAITSALN TLASYGYNLN TLGLGLSQAA ATGALAAAAA SANPAAAAAN 370 380 390 400 410 420 LLATYASEAS ASGSTAGGTA GTFALGSLAA ATAATNGYFG AASPLAASAI LGTEKSTDGS 430 440 450 460 470 480 KDVVEIAVPE NLVGAILGKG GKTLVEYQEL TGARIQISKK GEFVPGTRNR KVTITGTPAA 490 500 TQAAQYLITQ RITYEQGVRA ANPQKVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nova, the paraneoplastic Ri antigen, is homologous to an RNA-binding protein and is specifically expressed in the developing motor system." Buckanovich R.J., Posner J.B., Darnell R.B. Neuron 11:657-672(1993) [PubMed: 8398153] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3). Tissue: Cerebellum and Hippocampus.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4). Tissue: Brain.
[3]	Dmitrenko V.V., Garifulin O.M., Shostak K.A., Smikodub A.I., Kavsan V.M. Submitted (APR-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-34. Tissue: Fetal brain.
[4]	"Crystal structures of Nova-1 and Nova-2 K-homology RNA-binding domains." Lewis H.A., Chen H., Edo C., Buckanovich R.J., Yang Y.Y.-L., Musunuru K., Zhong R., Darnell R.B., Burley S.K. Structure 7:191-203(1999) [PubMed: 10368286] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 423-495.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U04840 mRNA. Translation: AAA16022.1.
BC075038 mRNA. Translation: AAH75038.1.
BC075039 mRNA. Translation: AAH75039.1.
Z70771 mRNA. Translation: CAA94810.1.

PIR

I38489.

RefSeq

NP_002506.2.
NP_006480.2.
NP_006482.1.

UniGene

Hs.31588

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1DT4	X-ray	2.60	A	424-495	[»]
2ANN	X-ray	2.30	A	49-249	[»]
2ANR	X-ray	1.94	A	49-249	[»]

SMR

P51513. Positions 424-509.

ModBase

Search...

Protein-protein interaction databases

IntAct

P51513.

PTM databases

PhosphoSite

P51513.

Genome annotation databases

Ensembl

ENSG00000139910. Homo sapiens. [Contig view]

GeneID

4857.

KEGG

hsa:4857.

Organism-specific databases

H-InvDB

HIX0037755.

HGNC

HGNC:7886. NOVA1.

HPA

HPA004155.

MIM

602157. gene.

PharmGKB

PA31688.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

P51513.

HOVERGEN

P51513.

Gene expression databases

ArrayExpress

P51513.

CleanEx

HS_NOVA1.

GermOnline

ENSG00000139910. Homo sapiens.

Family and domain databases

InterPro

IPR004087. KH.
IPR004088. KH_type_1.
[Graphical view]

Pfam

PF00013. KH_1. 3 hits.
[Graphical view]

SMART

SM00322. KH. 3 hits.
[Graphical view]

PROSITE

PS50084. KH_TYPE_1. 3 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

18710.

SOURCE

Search...

Entry information

Entry name

NOVA1_HUMAN

Accession

Primary (citable) accession number: P51513
Secondary accession number(s): Q6B004

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)