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P51489

- OPSD_RAT

UniProt

P51489 - OPSD_RAT

Protein

Rhodopsin

Gene

Rho

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal By similarity.By similarity

    Absorptioni

    Abs(max)=495 nm

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi201 – 2011ZincBy similarity
    Binding sitei265 – 2651Retinal chromophoreBy similarity
    Metal bindingi279 – 2791ZincBy similarity

    GO - Molecular functioni

    1. G-protein coupled receptor activity Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. photoreceptor activity Source: UniProtKB-KW
    4. retinal binding Source: RGD
    5. spectrin binding Source: MGI

    GO - Biological processi

    1. protein-chromophore linkage Source: UniProtKB-KW
    2. protein phosphorylation Source: Ensembl
    3. red, far-red light phototransduction Source: RGD
    4. retina development in camera-type eye Source: Ensembl
    5. rhodopsin mediated signaling pathway Source: RGD
    6. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    Chromophore, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_202899. Activation of the phototransduction cascade.
    REACT_209105. The canonical retinoid cycle in rods (twilight vision).
    REACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rhodopsin
    Gene namesi
    Name:Rho
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi3573. Rho.

    Subcellular locationi

    Membrane; Multi-pass membrane protein
    Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: Ensembl
    2. integral component of membrane Source: UniProtKB-KW
    3. photoreceptor inner segment membrane Source: UniProtKB
    4. photoreceptor outer segment Source: RGD
    5. photoreceptor outer segment membrane Source: UniProtKB
    6. plasma membrane Source: UniProtKB
    7. rough endoplasmic reticulum membrane Source: RGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 348348RhodopsinPRO_0000197708Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Glycosylationi2 – 21N-linked (GlcNAc...)By similarity
    Glycosylationi15 – 151N-linked (GlcNAc...)By similarity
    Disulfide bondi110 ↔ 187PROSITE-ProRule annotation
    Modified residuei296 – 2961N6-(retinylidene)lysineBy similarity
    Lipidationi322 – 3221S-palmitoyl cysteineBy similarity
    Lipidationi323 – 3231S-palmitoyl cysteineBy similarity
    Modified residuei334 – 3341PhosphoserineBy similarity
    Modified residuei336 – 3361PhosphothreonineBy similarity
    Modified residuei338 – 3381PhosphoserineBy similarity
    Modified residuei340 – 3401PhosphothreonineBy similarity
    Modified residuei342 – 3421PhosphothreonineBy similarity
    Modified residuei343 – 3431PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.By similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP51489.
    PRIDEiP51489.

    PTM databases

    PhosphoSiteiP51489.

    Expressioni

    Tissue specificityi

    Rod shaped photoreceptor cells which mediates vision in dim light.

    Gene expression databases

    GenevestigatoriP51489.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000061473.

    Structurei

    3D structure databases

    ProteinModelPortaliP51489.
    SMRiP51489. Positions 1-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3636ExtracellularAdd
    BLAST
    Topological domaini62 – 7312CytoplasmicAdd
    BLAST
    Topological domaini99 – 11315ExtracellularAdd
    BLAST
    Topological domaini134 – 15219CytoplasmicAdd
    BLAST
    Topological domaini177 – 20226ExtracellularAdd
    BLAST
    Topological domaini231 – 25222CytoplasmicAdd
    BLAST
    Topological domaini277 – 2848Extracellular
    Topological domaini310 – 34839CytoplasmicAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei37 – 6125Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei74 – 9825Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei114 – 13320Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei153 – 17624Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei203 – 23028Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei253 – 27624Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei285 – 30925Helical; Name=7Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni113 – 12513Retinal chromophore bindingBy similarityAdd
    BLAST
    Regioni207 – 2126Retinal chromophore bindingBy similarity

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi134 – 1374'Ionic lock' involved in activated form stabilization

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG311294.
    GeneTreeiENSGT00710000106574.
    HOGENOMiHOG000253932.
    HOVERGENiHBG107442.
    InParanoidiP51489.
    KOiK04250.
    OrthoDBiEOG72NRQJ.
    PhylomeDBiP51489.
    TreeFamiTF324998.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    4.10.840.10. 1 hit.
    InterProiIPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001760. Opsin.
    IPR027430. Retinal_BS.
    IPR000732. Rhodopsin.
    IPR019477. Rhodopsin_N.
    [Graphical view]
    PfamiPF00001. 7tm_1. 1 hit.
    PF10413. Rhodopsin_N. 1 hit.
    [Graphical view]
    PRINTSiPR00237. GPCRRHODOPSN.
    PR00238. OPSIN.
    PR00579. RHODOPSIN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    PS00238. OPSIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51489-1 [UniParc]FASTAAdd to Basket

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    MNGTEGPNFY VPFSNITGVV RSPFEQPQYY LAEPWQFSML AAYMFLLIVL    50
    GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH 100
    GYFVFGPTGC NLEGFFATLG GEIGLWSLVV LAIERYVVVC KPMSNFRFGE 150
    NHAIMGVAFT WVMALACAAP PLVGWSRYIP EGMQCSCGID YYTLKPEVNN 200
    ESFVIYMFVV HFTIPMIVIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV 250
    TRMVIIMVIF FLICWLPYAS VAMYIFTHQG SNFGPIFMTL PAFFAKTASI 300
    YNPIIYIMMN KQFRNCMLTS LCCGKNPLGD DEASATASKT ETSQVAPA 348
    Length:348
    Mass (Da):39,042
    Last modified:October 1, 1996 - v1
    Checksum:iDA0F3F90C30984BC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti320 – 3201S → T in CAA87081. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22180 Genomic DNA. Translation: AAA84439.1.
    Z46957 mRNA. Translation: CAA87081.1.
    PIRiS51677.
    RefSeqiNP_254276.1. NM_033441.1.
    UniGeneiRn.92530.

    Genome annotation databases

    EnsembliENSRNOT00000064603; ENSRNOP00000061473; ENSRNOG00000011144.
    GeneIDi24717.
    KEGGirno:24717.
    UCSCiRGD:3573. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U22180 Genomic DNA. Translation: AAA84439.1 .
    Z46957 mRNA. Translation: CAA87081.1 .
    PIRi S51677.
    RefSeqi NP_254276.1. NM_033441.1.
    UniGenei Rn.92530.

    3D structure databases

    ProteinModelPortali P51489.
    SMRi P51489. Positions 1-348.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000061473.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P51489.

    Proteomic databases

    PaxDbi P51489.
    PRIDEi P51489.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000064603 ; ENSRNOP00000061473 ; ENSRNOG00000011144 .
    GeneIDi 24717.
    KEGGi rno:24717.
    UCSCi RGD:3573. rat.

    Organism-specific databases

    CTDi 6010.
    RGDi 3573. Rho.

    Phylogenomic databases

    eggNOGi NOG311294.
    GeneTreei ENSGT00710000106574.
    HOGENOMi HOG000253932.
    HOVERGENi HBG107442.
    InParanoidi P51489.
    KOi K04250.
    OrthoDBi EOG72NRQJ.
    PhylomeDBi P51489.
    TreeFami TF324998.

    Enzyme and pathway databases

    Reactomei REACT_202899. Activation of the phototransduction cascade.
    REACT_209105. The canonical retinoid cycle in rods (twilight vision).
    REACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.

    Miscellaneous databases

    NextBioi 604217.
    PROi P51489.

    Gene expression databases

    Genevestigatori P51489.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    4.10.840.10. 1 hit.
    InterProi IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    IPR001760. Opsin.
    IPR027430. Retinal_BS.
    IPR000732. Rhodopsin.
    IPR019477. Rhodopsin_N.
    [Graphical view ]
    Pfami PF00001. 7tm_1. 1 hit.
    PF10413. Rhodopsin_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00237. GPCRRHODOPSN.
    PR00238. OPSIN.
    PR00579. RHODOPSIN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    PS00238. OPSIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and coding sequence of the rat rod opsin gene."
      Barnstable C.J., Morabito M.A.
      J. Mol. Neurosci. 5:207-209(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Retinal rod cell.
    2. Huber A., Baker B.B., Sander P., Gerdon G., Paulsen R., Williams T.P.
      Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Retina.

    Entry informationi

    Entry nameiOPSD_RAT
    AccessioniPrimary (citable) accession number: P51489
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3