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P51489

- OPSD_RAT

UniProt

P51489 - OPSD_RAT

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Protein

Rhodopsin

Gene

Rho

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal (By similarity).By similarity

Absorptioni

Abs(max)=495 nm

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi201 – 2011ZincBy similarity
Binding sitei265 – 2651Retinal chromophoreBy similarity
Metal bindingi279 – 2791ZincBy similarity

GO - Molecular functioni

  1. G-protein coupled receptor activity Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. photoreceptor activity Source: UniProtKB-KW
  4. retinal binding Source: RGD
  5. spectrin binding Source: MGI

GO - Biological processi

  1. protein-chromophore linkage Source: UniProtKB-KW
  2. protein phosphorylation Source: Ensembl
  3. red, far-red light phototransduction Source: RGD
  4. retina development in camera-type eye Source: Ensembl
  5. rhodopsin mediated signaling pathway Source: RGD
  6. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Photoreceptor protein, Receptor, Retinal protein, Transducer

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Chromophore, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_202899. Activation of the phototransduction cascade.
REACT_209105. The canonical retinoid cycle in rods (twilight vision).
REACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_233455. G alpha (i) signalling events.
REACT_252226. Opsins.

Names & Taxonomyi

Protein namesi
Recommended name:
Rhodopsin
Gene namesi
Name:Rho
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi3573. Rho.

Subcellular locationi

Membrane; Multi-pass membrane protein
Note: Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3636ExtracellularAdd
BLAST
Transmembranei37 – 6125Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini62 – 7312CytoplasmicAdd
BLAST
Transmembranei74 – 9825Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini99 – 11315ExtracellularAdd
BLAST
Transmembranei114 – 13320Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini134 – 15219CytoplasmicAdd
BLAST
Transmembranei153 – 17624Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini177 – 20226ExtracellularAdd
BLAST
Transmembranei203 – 23028Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini231 – 25222CytoplasmicAdd
BLAST
Transmembranei253 – 27624Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini277 – 2848Extracellular
Transmembranei285 – 30925Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini310 – 34839CytoplasmicAdd
BLAST

GO - Cellular componenti

  1. cell-cell junction Source: Ensembl
  2. Golgi apparatus Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
  4. photoreceptor inner segment membrane Source: UniProtKB
  5. photoreceptor outer segment Source: RGD
  6. photoreceptor outer segment membrane Source: UniProtKB
  7. plasma membrane Source: UniProtKB
  8. rough endoplasmic reticulum membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 348348RhodopsinPRO_0000197708Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Glycosylationi2 – 21N-linked (GlcNAc...)By similarity
Glycosylationi15 – 151N-linked (GlcNAc...)By similarity
Disulfide bondi110 ↔ 187PROSITE-ProRule annotation
Modified residuei296 – 2961N6-(retinylidene)lysineBy similarity
Lipidationi322 – 3221S-palmitoyl cysteineBy similarity
Lipidationi323 – 3231S-palmitoyl cysteineBy similarity
Modified residuei334 – 3341PhosphoserineBy similarity
Modified residuei336 – 3361PhosphothreonineBy similarity
Modified residuei338 – 3381PhosphoserineBy similarity
Modified residuei340 – 3401PhosphothreonineBy similarity
Modified residuei342 – 3421PhosphothreonineBy similarity
Modified residuei343 – 3431PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.By similarity

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP51489.
PRIDEiP51489.

PTM databases

PhosphoSiteiP51489.

Expressioni

Tissue specificityi

Rod shaped photoreceptor cells which mediates vision in dim light.

Gene expression databases

GenevestigatoriP51489.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000061473.

Structurei

3D structure databases

ProteinModelPortaliP51489.
SMRiP51489. Positions 1-348.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni113 – 12513Retinal chromophore bindingBy similarityAdd
BLAST
Regioni207 – 2126Retinal chromophore bindingBy similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi134 – 1374'Ionic lock' involved in activated form stabilization

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311294.
GeneTreeiENSGT00760000118977.
HOGENOMiHOG000253932.
HOVERGENiHBG107442.
InParanoidiP51489.
KOiK04250.
OrthoDBiEOG72NRQJ.
PhylomeDBiP51489.
TreeFamiTF324998.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
4.10.840.10. 1 hit.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR027430. Retinal_BS.
IPR000732. Rhodopsin.
IPR019477. Rhodopsin_N.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF10413. Rhodopsin_N. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PR00579. RHODOPSIN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51489-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNGTEGPNFY VPFSNITGVV RSPFEQPQYY LAEPWQFSML AAYMFLLIVL
60 70 80 90 100
GFPINFLTLY VTVQHKKLRT PLNYILLNLA VADLFMVFGG FTTTLYTSLH
110 120 130 140 150
GYFVFGPTGC NLEGFFATLG GEIGLWSLVV LAIERYVVVC KPMSNFRFGE
160 170 180 190 200
NHAIMGVAFT WVMALACAAP PLVGWSRYIP EGMQCSCGID YYTLKPEVNN
210 220 230 240 250
ESFVIYMFVV HFTIPMIVIF FCYGQLVFTV KEAAAQQQES ATTQKAEKEV
260 270 280 290 300
TRMVIIMVIF FLICWLPYAS VAMYIFTHQG SNFGPIFMTL PAFFAKTASI
310 320 330 340
YNPIIYIMMN KQFRNCMLTS LCCGKNPLGD DEASATASKT ETSQVAPA
Length:348
Mass (Da):39,042
Last modified:October 1, 1996 - v1
Checksum:iDA0F3F90C30984BC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti320 – 3201S → T in CAA87081. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22180 Genomic DNA. Translation: AAA84439.1.
Z46957 mRNA. Translation: CAA87081.1.
PIRiS51677.
RefSeqiNP_254276.1. NM_033441.1.
UniGeneiRn.92530.

Genome annotation databases

EnsembliENSRNOT00000064603; ENSRNOP00000061473; ENSRNOG00000011144.
GeneIDi24717.
KEGGirno:24717.
UCSCiRGD:3573. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U22180 Genomic DNA. Translation: AAA84439.1 .
Z46957 mRNA. Translation: CAA87081.1 .
PIRi S51677.
RefSeqi NP_254276.1. NM_033441.1.
UniGenei Rn.92530.

3D structure databases

ProteinModelPortali P51489.
SMRi P51489. Positions 1-348.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000061473.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P51489.

Proteomic databases

PaxDbi P51489.
PRIDEi P51489.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000064603 ; ENSRNOP00000061473 ; ENSRNOG00000011144 .
GeneIDi 24717.
KEGGi rno:24717.
UCSCi RGD:3573. rat.

Organism-specific databases

CTDi 6010.
RGDi 3573. Rho.

Phylogenomic databases

eggNOGi NOG311294.
GeneTreei ENSGT00760000118977.
HOGENOMi HOG000253932.
HOVERGENi HBG107442.
InParanoidi P51489.
KOi K04250.
OrthoDBi EOG72NRQJ.
PhylomeDBi P51489.
TreeFami TF324998.

Enzyme and pathway databases

Reactomei REACT_202899. Activation of the phototransduction cascade.
REACT_209105. The canonical retinoid cycle in rods (twilight vision).
REACT_227662. Inactivation, recovery and regulation of the phototransduction cascade.
REACT_233455. G alpha (i) signalling events.
REACT_252226. Opsins.

Miscellaneous databases

NextBioi 604217.
PROi P51489.

Gene expression databases

Genevestigatori P51489.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
4.10.840.10. 1 hit.
InterProi IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR001760. Opsin.
IPR027430. Retinal_BS.
IPR000732. Rhodopsin.
IPR019477. Rhodopsin_N.
[Graphical view ]
Pfami PF00001. 7tm_1. 1 hit.
PF10413. Rhodopsin_N. 1 hit.
[Graphical view ]
PRINTSi PR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PR00579. RHODOPSIN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Isolation and coding sequence of the rat rod opsin gene."
    Barnstable C.J., Morabito M.A.
    J. Mol. Neurosci. 5:207-209(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Retinal rod cell.
  2. Huber A., Baker B.B., Sander P., Gerdon G., Paulsen R., Williams T.P.
    Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Retina.

Entry informationi

Entry nameiOPSD_RAT
AccessioniPrimary (citable) accession number: P51489
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3