P51475 (OPSP_CHICK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Pinopsin Alternative name(s): Pineal gland-specific opsin Short name=P-opsin Short name=Pineal opsin
Organism	Gallus gallus (Chicken) [Reference proteome]
Taxonomic identifier	9031 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Sauropsida › Sauria › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Produces a slow and prolonged phototransduction response consistent with the non-visual function of pineal photoreception.
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Pineal gland.
Post-translational modification	Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region By similarity.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
Biophysicochemical properties	Absorption: Abs(max)=~470 nm

Ontologies

Keywords
Biological process	Sensory transduction
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Chromophore
Molecular function	G-protein coupled receptor Photoreceptor protein Receptor Retinal protein Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phototransduction Inferred from electronic annotation. Source: UniProtKB-KW protein-chromophore linkage Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor outer segment Inferred from electronic annotation. Source: Compara
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW photoreceptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 351	351	Pinopsin		PRO_0000197807
Regions
	Topological domain	1 – 30	30	Extracellular
	Transmembrane	31 – 55	25	Helical; Name=1; Potential
	Topological domain	56 – 67	12	Cytoplasmic
	Transmembrane	68 – 92	25	Helical; Name=2; Potential
	Topological domain	93 – 107	15	Extracellular
	Transmembrane	108 – 127	20	Helical; Name=3; Potential
	Topological domain	128 – 146	19	Cytoplasmic
	Transmembrane	147 – 170	24	Helical; Name=4; Potential
	Topological domain	171 – 194	24	Extracellular
	Transmembrane	195 – 222	28	Helical; Name=5; Potential
	Topological domain	223 – 244	22	Cytoplasmic
	Transmembrane	245 – 268	24	Helical; Name=6; Potential
	Topological domain	269 – 276	8	Extracellular
	Transmembrane	277 – 301	25	Helical; Name=7; Potential
	Topological domain	302 – 351	50	Cytoplasmic
Amino acid modifications
	Modified residue	288	1	N6-(retinylidene)lysine
	Lipidation	314	1	S-palmitoyl cysteine By similarity
	Lipidation	315	1	S-palmitoyl cysteine By similarity
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Glycosylation	192	1	N-linked (GlcNAc...) Potential
	Disulfide bond	104 ↔ 181		By similarity
Experimental info
	Sequence conflict	28	1	Q → W in AAB47565. Ref.3
	Sequence conflict	135	1	K → R in AAA64223. Ref.2
	Sequence conflict	163	1	A → T in AAA64223. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P51475 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 56BDFAD187008338	FASTA	351	38,174
	10 20 30 40 50 60 MSSNSSQAPP NGTPGPFDGP QWPYQAPQST YVGVAVLMGT VVACASVVNG LVIVVSICYK 70 80 90 100 110 120 KLRSPLNYIL VNLAVADLLV TLCGSSVSLS NNINGFFVFG RRMCELEGFM VSLTGIVGLW 130 140 150 160 170 180 SLAILALERY VVVCKPLGDF QFQRRHAVSG CAFTWGWALL WSAPPLLGWS SYVPEGLRTS 190 200 210 220 230 240 CGPNWYTGGS NNNSYILSLF VTCFVLPLSL ILFSYTNLLL TLRAAAAQQK EADTTQRAER 250 260 270 280 290 300 EVTRMVIVMV MAFLLCWLPY STFALVVATH KGIIIQPVLA SLPSYFSKTA TVYNPIIYVF 310 320 330 340 350 MNKQFQSCLL EMLCCGYQPQ RTGKASPGTP GPHADVTAAG LRNKVMPAHP V

References

[1]	"Pineal opsin: a nonvisual opsin expressed in chick pineal." Max M., McKinnon P.J., Seidenman K.J., Barrett R.K., Applebury M.L., Takahashi J.S., Margolskee R.F. Science 267:1502-1506(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Tissue: Pineal gland.
[2]	"Pinopsin is a chicken pineal photoreceptive molecule." Okano T., Yoshizawa T., Fukada Y. Nature 372:94-97(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pineal gland.
[3]	"Light-dependent activation of rod transducin by pineal opsin." Max M., Surya A., Takahashi J.S., Margolskee R.F., Knox B.E. J. Biol. Chem. 273:26820-26826(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: White leghorn. Tissue: Pineal gland.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U15762 mRNA. Translation: AAA64223.1. U87449 Genomic DNA. Translation: AAB47565.1.
IPI	IPI00596875.
PIR	A55962.
RefSeq	NP_990740.1. NM_205409.1.
UniGene	Gga.786.
3D structure databases
ProteinModelPortal	P51475.
ModBase	Search...
Protein-protein interaction databases
STRING	9031.ENSGALP00000007864.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PaxDb	P51475.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSGALT00000007878; ENSGALP00000007864; ENSGALG00000004924.
GeneID	396377.
KEGG	gga:396377.
Organism-specific databases
CTD	5956.
Phylogenomic databases
eggNOG	NOG240324.
GeneTree	ENSGT00700000104057.
HOGENOM	HOG000253932.
HOVERGEN	HBG107442.
InParanoid	P51475.
KO	K04251.
OrthoDB	EOG4HMJ9W.
Family and domain databases
InterPro	IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. IPR001760. Opsin. IPR002206. Opsin_pineal. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00238. OPSIN. PR00666. PINOPSIN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. PS00238. OPSIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20816419.

Entry information

Entry name

OPSP_CHICK

Accession

Primary (citable) accession number: P51475
Secondary accession number(s): P79794

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program