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Protein

Dual specificity protein phosphatase 3

Gene

DUSP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124Phosphocysteine intermediate1

GO - Molecular functioni

  • MAP kinase phosphatase activity Source: UniProtKB
  • phosphatase activity Source: UniProtKB
  • protein kinase binding Source: MGI
  • protein tyrosine/serine/threonine phosphatase activity Source: UniProtKB
  • protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  • dephosphorylation Source: UniProtKB
  • inactivation of MAPK activity Source: UniProtKB
  • in utero embryonic development Source: Ensembl
  • negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • negative regulation of JNK cascade Source: UniProtKB
  • negative regulation of MAPK cascade Source: UniProtKB
  • negative regulation of T cell activation Source: UniProtKB
  • negative regulation of T cell receptor signaling pathway Source: UniProtKB
  • peptidyl-tyrosine dephosphorylation Source: UniProtKB
  • positive regulation of mitotic cell cycle Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protein phosphatase

Enzyme and pathway databases

ReactomeiR-HSA-202670 ERKs are inactivated
SignaLinkiP51452
SIGNORiP51452

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 3 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Dual specificity protein phosphatase VHR
Vaccinia H1-related phosphatase
Short name:
VHR
Gene namesi
Name:DUSP3
Synonyms:VHR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

EuPathDBiHostDB:ENSG00000108861.8
HGNCiHGNC:3069 DUSP3
MIMi600183 gene
neXtProtiNX_P51452

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1845
OpenTargetsiENSG00000108861
PharmGKBiPA27526

Chemistry databases

ChEMBLiCHEMBL2635

Polymorphism and mutation databases

BioMutaiDUSP3
DMDMi1718191

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000947951 – 185Dual specificity protein phosphatase 3Add BLAST185

Proteomic databases

EPDiP51452
MaxQBiP51452
PaxDbiP51452
PeptideAtlasiP51452
PRIDEiP51452
ProteomicsDBi56308

2D gel databases

UCD-2DPAGEiP51452

PTM databases

DEPODiP51452
iPTMnetiP51452
PhosphoSitePlusiP51452

Expressioni

Gene expression databases

BgeeiENSG00000108861
CleanExiHS_DUSP3
ExpressionAtlasiP51452 baseline and differential
GenevisibleiP51452 HS

Organism-specific databases

HPAiHPA063616

Interactioni

Subunit structurei

Interacts with VRK3, which seems to activate it's phosphatase activity.By similarity

GO - Molecular functioni

  • protein kinase binding Source: MGI

Protein-protein interaction databases

BioGridi108178, 36 interactors
IntActiP51452, 7 interactors
MINTiP51452
STRINGi9606.ENSP00000226004

Chemistry databases

BindingDBiP51452

Structurei

Secondary structure

1185
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 16Combined sources9
Beta strandi19 – 21Combined sources3
Beta strandi27 – 34Combined sources8
Beta strandi37 – 40Combined sources4
Helixi42 – 45Combined sources4
Helixi48 – 54Combined sources7
Beta strandi58 – 61Combined sources4
Beta strandi64 – 67Combined sources4
Helixi76 – 78Combined sources3
Turni79 – 82Combined sources4
Beta strandi84 – 87Combined sources4
Helixi98 – 101Combined sources4
Helixi102 – 114Combined sources13
Turni115 – 117Combined sources3
Beta strandi120 – 123Combined sources4
Beta strandi125 – 129Combined sources5
Helixi130 – 143Combined sources14
Helixi147 – 157Combined sources11
Helixi164 – 179Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J4XX-ray2.75A2-185[»]
1VHRX-ray2.10A/B2-185[»]
3F81X-ray1.90A/B3-185[»]
ProteinModelPortaliP51452
SMRiP51452
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51452

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 169Tyrosine-protein phosphataseAdd BLAST71

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716 Eukaryota
COG2453 LUCA
GeneTreeiENSGT00760000118853
HOGENOMiHOG000233767
HOVERGENiHBG001524
InParanoidiP51452
KOiK17614
OMAiQLCQLNE
OrthoDBiEOG091G0249
PhylomeDBiP51452
TreeFamiTF105128

Family and domain databases

CDDicd00127 DSPc, 1 hit
Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR020417 Atypical_DUSP
IPR020405 Atypical_DUSP_famA
IPR000340 Dual-sp_phosphatase_cat-dom
IPR024950 DUSP
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
IPR020422 TYR_PHOSPHATASE_DUAL_dom
PANTHERiPTHR10159 PTHR10159, 1 hit
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PRINTSiPR01908 ADSPHPHTASE
PR01909 ADSPHPHTASEA
SMARTiView protein in SMART
SM00195 DSPc, 1 hit
SUPFAMiSSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
PS50054 TYR_PHOSPHATASE_DUAL, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51452-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK
60 70 80 90 100
LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA
110 120 130 140 150
YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA
160 170 180
LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP
Length:185
Mass (Da):20,478
Last modified:October 1, 1996 - v1
Checksum:iC1045DD9B226FD94
GO
Isoform 2 (identifier: P51452-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: MSGSFELSVQDLNDLLSDGSGCYSLPSQPCNEVTPRIYVGNA → M

Note: No experimental confirmation available.
Show »
Length:144
Mass (Da):16,163
Checksum:i21CA189AF19D20B3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0562841 – 42MSGSF…YVGNA → M in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05147 mRNA Translation: AAA35777.1
BT019522 mRNA Translation: AAV38329.1
CH471178 Genomic DNA Translation: EAW51667.1
CH471178 Genomic DNA Translation: EAW51668.1
BC002682 mRNA Translation: AAH02682.1
BC035701 mRNA Translation: AAH35701.1
AC003098 Genomic DNA No translation available.
AC055813 Genomic DNA No translation available.
CCDSiCCDS11469.1 [P51452-1]
PIRiA47196
RefSeqiNP_004081.1, NM_004090.3 [P51452-1]
UniGeneiHs.181046

Genome annotation databases

EnsembliENST00000226004; ENSP00000226004; ENSG00000108861 [P51452-1]
GeneIDi1845
KEGGihsa:1845
UCSCiuc002ied.5 human [P51452-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDUS3_HUMAN
AccessioniPrimary (citable) accession number: P51452
Secondary accession number(s): D3DX45, Q5U0J1, Q8IYJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 20, 2018
This is version 171 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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