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Protein

Dual specificity protein phosphatase 3

Gene

DUSP3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Shows activity both for tyrosine-protein phosphate and serine-protein phosphate, but displays a strong preference toward phosphotyrosines. Specifically dephosphorylates and inactivates ERK1 and ERK2.2 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation
[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei124Phosphocysteine intermediate1

GO - Molecular functioni

  • MAP kinase phosphatase activity Source: UniProtKB
  • phosphatase activity Source: UniProtKB
  • protein kinase binding Source: MGI
  • protein tyrosine/serine/threonine phosphatase activity Source: UniProtKB
  • protein tyrosine phosphatase activity Source: UniProtKB

GO - Biological processi

  • dephosphorylation Source: UniProtKB
  • inactivation of MAPK activity Source: UniProtKB
  • in utero embryonic development Source: Ensembl
  • negative regulation of ERK1 and ERK2 cascade Source: UniProtKB
  • negative regulation of JNK cascade Source: UniProtKB
  • negative regulation of MAPK cascade Source: UniProtKB
  • negative regulation of T cell activation Source: UniProtKB
  • negative regulation of T cell receptor signaling pathway Source: UniProtKB
  • peptidyl-tyrosine dephosphorylation Source: UniProtKB
  • positive regulation of mitotic cell cycle Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Enzyme and pathway databases

BioCyciZFISH:HS03174-MONOMER.
ReactomeiR-HSA-202670. ERKs are inactivated.
SignaLinkiP51452.
SIGNORiP51452.

Names & Taxonomyi

Protein namesi
Recommended name:
Dual specificity protein phosphatase 3 (EC:3.1.3.16, EC:3.1.3.48)
Alternative name(s):
Dual specificity protein phosphatase VHR
Vaccinia H1-related phosphatase
Short name:
VHR
Gene namesi
Name:DUSP3
Synonyms:VHR
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:3069. DUSP3.

Subcellular locationi

  • Nucleus 1 Publication

GO - Cellular componenti

  • cytoplasm Source: HPA
  • cytosol Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • immunological synapse Source: UniProtKB
  • nucleoplasm Source: HPA
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi1845.
OpenTargetsiENSG00000108861.
PharmGKBiPA27526.

Chemistry databases

ChEMBLiCHEMBL2635.

Polymorphism and mutation databases

BioMutaiDUSP3.
DMDMi1718191.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000947951 – 185Dual specificity protein phosphatase 3Add BLAST185

Proteomic databases

EPDiP51452.
MaxQBiP51452.
PaxDbiP51452.
PeptideAtlasiP51452.
PRIDEiP51452.

2D gel databases

UCD-2DPAGEP51452.

PTM databases

DEPODiP51452.
iPTMnetiP51452.
PhosphoSitePlusiP51452.

Expressioni

Gene expression databases

BgeeiENSG00000108861.
CleanExiHS_DUSP3.
ExpressionAtlasiP51452. baseline and differential.
GenevisibleiP51452. HS.

Organism-specific databases

HPAiHPA063616.

Interactioni

Subunit structurei

Interacts with VRK3, which seems to activate it's phosphatase activity.By similarity

GO - Molecular functioni

  • protein kinase binding Source: MGI

Protein-protein interaction databases

BioGridi108178. 15 interactors.
IntActiP51452. 6 interactors.
MINTiMINT-5001069.
STRINGi9606.ENSP00000226004.

Chemistry databases

BindingDBiP51452.

Structurei

Secondary structure

1185
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 16Combined sources9
Beta strandi19 – 21Combined sources3
Beta strandi27 – 34Combined sources8
Beta strandi37 – 40Combined sources4
Helixi42 – 45Combined sources4
Helixi48 – 54Combined sources7
Beta strandi58 – 61Combined sources4
Beta strandi64 – 67Combined sources4
Helixi76 – 78Combined sources3
Turni79 – 82Combined sources4
Beta strandi84 – 87Combined sources4
Helixi98 – 101Combined sources4
Helixi102 – 114Combined sources13
Turni115 – 117Combined sources3
Beta strandi120 – 123Combined sources4
Beta strandi125 – 129Combined sources5
Helixi130 – 143Combined sources14
Helixi147 – 157Combined sources11
Helixi164 – 179Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J4XX-ray2.75A2-185[»]
1VHRX-ray2.10A/B2-185[»]
3F81X-ray1.90A/B3-185[»]
ProteinModelPortaliP51452.
SMRiP51452.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51452.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 169Tyrosine-protein phosphataseAdd BLAST71

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000233767.
HOVERGENiHBG001524.
InParanoidiP51452.
KOiK17614.
OMAiQKMDVRS.
OrthoDBiEOG091G0249.
PhylomeDBiP51452.
TreeFamiTF105128.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020405. Atypical_DUSP_famA.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01909. ADSPHPHTASEA.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P51452-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK
60 70 80 90 100
LQKLGITHVL NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA
110 120 130 140 150
YFERAADFID QALAQKNGRV LVHCREGYSR SPTLVIAYLM MRQKMDVKSA
160 170 180
LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP
Length:185
Mass (Da):20,478
Last modified:October 1, 1996 - v1
Checksum:iC1045DD9B226FD94
GO
Isoform 2 (identifier: P51452-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-42: MSGSFELSVQDLNDLLSDGSGCYSLPSQPCNEVTPRIYVGNA → M

Note: No experimental confirmation available.
Show »
Length:144
Mass (Da):16,163
Checksum:i21CA189AF19D20B3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0562841 – 42MSGSF…YVGNA → M in isoform 2. 1 PublicationAdd BLAST42

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05147 mRNA. Translation: AAA35777.1.
BT019522 mRNA. Translation: AAV38329.1.
CH471178 Genomic DNA. Translation: EAW51667.1.
CH471178 Genomic DNA. Translation: EAW51668.1.
BC002682 mRNA. Translation: AAH02682.1.
BC035701 mRNA. Translation: AAH35701.1.
AC003098 Genomic DNA. No translation available.
AC055813 Genomic DNA. No translation available.
CCDSiCCDS11469.1. [P51452-1]
PIRiA47196.
RefSeqiNP_004081.1. NM_004090.3. [P51452-1]
UniGeneiHs.181046.

Genome annotation databases

EnsembliENST00000226004; ENSP00000226004; ENSG00000108861. [P51452-1]
GeneIDi1845.
KEGGihsa:1845.
UCSCiuc002ied.5. human. [P51452-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05147 mRNA. Translation: AAA35777.1.
BT019522 mRNA. Translation: AAV38329.1.
CH471178 Genomic DNA. Translation: EAW51667.1.
CH471178 Genomic DNA. Translation: EAW51668.1.
BC002682 mRNA. Translation: AAH02682.1.
BC035701 mRNA. Translation: AAH35701.1.
AC003098 Genomic DNA. No translation available.
AC055813 Genomic DNA. No translation available.
CCDSiCCDS11469.1. [P51452-1]
PIRiA47196.
RefSeqiNP_004081.1. NM_004090.3. [P51452-1]
UniGeneiHs.181046.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J4XX-ray2.75A2-185[»]
1VHRX-ray2.10A/B2-185[»]
3F81X-ray1.90A/B3-185[»]
ProteinModelPortaliP51452.
SMRiP51452.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108178. 15 interactors.
IntActiP51452. 6 interactors.
MINTiMINT-5001069.
STRINGi9606.ENSP00000226004.

Chemistry databases

BindingDBiP51452.
ChEMBLiCHEMBL2635.

PTM databases

DEPODiP51452.
iPTMnetiP51452.
PhosphoSitePlusiP51452.

Polymorphism and mutation databases

BioMutaiDUSP3.
DMDMi1718191.

2D gel databases

UCD-2DPAGEP51452.

Proteomic databases

EPDiP51452.
MaxQBiP51452.
PaxDbiP51452.
PeptideAtlasiP51452.
PRIDEiP51452.

Protocols and materials databases

DNASUi1845.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000226004; ENSP00000226004; ENSG00000108861. [P51452-1]
GeneIDi1845.
KEGGihsa:1845.
UCSCiuc002ied.5. human. [P51452-1]

Organism-specific databases

CTDi1845.
DisGeNETi1845.
GeneCardsiDUSP3.
H-InvDBHIX0013868.
HGNCiHGNC:3069. DUSP3.
HPAiHPA063616.
MIMi600183. gene.
neXtProtiNX_P51452.
OpenTargetsiENSG00000108861.
PharmGKBiPA27526.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1716. Eukaryota.
COG2453. LUCA.
GeneTreeiENSGT00760000118853.
HOGENOMiHOG000233767.
HOVERGENiHBG001524.
InParanoidiP51452.
KOiK17614.
OMAiQKMDVRS.
OrthoDBiEOG091G0249.
PhylomeDBiP51452.
TreeFamiTF105128.

Enzyme and pathway databases

BioCyciZFISH:HS03174-MONOMER.
ReactomeiR-HSA-202670. ERKs are inactivated.
SignaLinkiP51452.
SIGNORiP51452.

Miscellaneous databases

ChiTaRSiDUSP3. human.
EvolutionaryTraceiP51452.
GeneWikiiDUSP3.
GenomeRNAii1845.
PROiP51452.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000108861.
CleanExiHS_DUSP3.
ExpressionAtlasiP51452. baseline and differential.
GenevisibleiP51452. HS.

Family and domain databases

CDDicd00127. DSPc. 1 hit.
Gene3Di3.90.190.10. 1 hit.
InterProiIPR020417. Atypical_DUSP.
IPR020405. Atypical_DUSP_famA.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR024950. DUSP.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR016130. Tyr_Pase_AS.
IPR000387. TYR_PHOSPHATASE_dom.
IPR020422. TYR_PHOSPHATASE_DUAL_dom.
[Graphical view]
PANTHERiPTHR10159. PTHR10159. 1 hit.
PfamiPF00782. DSPc. 1 hit.
[Graphical view]
PRINTSiPR01908. ADSPHPHTASE.
PR01909. ADSPHPHTASEA.
SMARTiSM00195. DSPc. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 1 hit.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDUS3_HUMAN
AccessioniPrimary (citable) accession number: P51452
Secondary accession number(s): D3DX45, Q5U0J1, Q8IYJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 161 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.