Reviewed, UniProtKB/Swiss-Prot P51452 (DUS3_HUMAN)

Names and origin

Protein names

Recommended name:
    Dual specificity protein phosphatase 3
    EC=3.1.3.48
    EC=3.1.3.16
Alternative name(s):
    Dual specificity protein phosphatase VHR

Gene names

Name:	DUSP3
Synonyms:	VHR

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	185 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein shows activity both toward tyrosine-protein phosphate as well as with serine-protein phosphate.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Sequence similarities	Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. Contains 1 tyrosine-protein phosphatase domain.

Ontologies

Keywords
Molecular function	Hydrolase Protein phosphatase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Ref.1 Traceable author statement. Source: ProtInc
Cellular component	nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	protein tyrosine phosphatase activity Ref.1 Inferred from Experiment. Source: Reactome
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 185

185

Dual specificity protein phosphatase 3

PRO_0000094795

Regions

Domain

99 – 169

71

Tyrosine-protein phosphatase

Sites

Active site

124

1

Phosphocysteine intermediate

Secondary structure

1

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185

Helix Strand Turn

Details...

Helix

9 – 16

8

Beta strand

17 – 21

5

Beta strand

27 – 34

8

Beta strand

37 – 40

4

Helix

42 – 45

4

Helix

48 – 54

7

Beta strand

58 – 61

4

Beta strand

64 – 67

4

Helix

75 – 78

4

Turn

79 – 82

4

Beta strand

84 – 87

4

Helix

98 – 101

4

Helix

102 – 113

12

Beta strand

120 – 123

4

Beta strand

125 – 129

5

Helix

130 – 142

13

Helix

147 – 157

11

Helix

164 – 179

16

Sequences

	Sequence		Length	Mass (Da)
	P51452-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: C1045DD9B226FD94	FASTA	185	20,478
	10 20 30 40 50 60 MSGSFELSVQ DLNDLLSDGS GCYSLPSQPC NEVTPRIYVG NASVAQDIPK LQKLGITHVL 70 80 90 100 110 120 NAAEGRSFMH VNTNANFYKD SGITYLGIKA NDTQEFNLSA YFERAADFID QALAQKNGRV 130 140 150 160 170 180 LVHCREGYSR SPTLVIAYLM MRQKMDVKSA LSIVRQNREI GPNDGFLAQL CQLNDRLAKE GKLKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Expression cloning of a human dual-specificity phosphatase." Ishibashi T., Bottaro D.P., Chan A., Miki T., Aaronson S.A. Proc. Natl. Acad. Sci. U.S.A. 89:12170-12174(1992) [PubMed: 1281549] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[3]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[4]	"Crystal structure of the dual specificity protein phosphatase VHR." Yuvaniyama J., Denu J.M., Dixon J.E., Saper M.A. Science 272:1328-1331(1996) [PubMed: 8650541] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

L05147 mRNA. Translation: AAA35777.1.
BC002682 mRNA. Translation: AAH02682.1.

IPI

IPI00018671.

PIR

A47196.

RefSeq

NP_004081.1.

UniGene

Hs.695925

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J4X	X-ray	2.75	A	2-185	[»]
1VHR	X-ray	2.10	A/B	2-185	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P51452. 2 interactions.

PTM databases

PhosphoSite

P51452.

Proteomic databases

PRIDE

P51452.

Genome annotation databases

Ensembl

ENSG00000108861. Homo sapiens. [Contig view]

GeneID

1845.

KEGG

hsa:1845.

Organism-specific databases

GeneCards

GC17M039199.

H-InvDB

HIX0013868.

HGNC

HGNC:3069. DUSP3.

MIM

600183. gene.

PharmGKB

PA27526.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P51452.

HOVERGEN

P51452.

OMA

P51452. YLMLRQK.

Enzyme and pathway databases

BRENDA

3.1.3.16. 247.
3.1.3.48. 247.

Reactome

REACT_11061. Signalling by NGF.

Gene expression databases

ArrayExpress

P51452.

Bgee

P51452.

CleanEx

HS_DUSP3.

GermOnline

ENSG00000108861. Homo sapiens.

Family and domain databases

InterPro

IPR000387. Tyr_Pase.
IPR016130. Tyr_Pase_AS.
IPR000340. Tyr_Pase_dual_specific.
[Graphical view]

Pfam

PF00782. DSPc. 1 hit.
[Graphical view]

SMART

SM00195. DSPc. 1 hit.
[Graphical view]

PROSITE

PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
PS50054. TYR_PHOSPHATASE_DUAL. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

7555.

SOURCE

Search...

Entry information

Entry name

DUS3_HUMAN

Accession

Primary (citable) accession number: P51452

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)