Reviewed, UniProtKB/Swiss-Prot P51451 (BLK_HUMAN)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase BLK
    EC=2.7.10.2
Alternative name(s):
    B lymphocyte kinase
    p55-BLK

Gene names

Name:

BLK

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	505 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May function in a signal transduction pathway that is restricted to B-lymphoid cells.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. Contains 1 protein kinase domain. Contains 1 SH2 domain. Contains 1 SH3 domain.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	SH2 domain SH3 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Lipoprotein Myristate Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro protein kinase cascade Traceable author statement. Source: ProtInc
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 505	504	Tyrosine-protein kinase BLK		PRO_0000088061
Regions
	Domain	58 – 118	61	SH3
	Domain	124 – 220	97	SH2
	Domain	241 – 494	254	Protein kinase
	Nucleotide binding	247 – 255	9	ATP By similarity
Sites
	Active site	360	1	Proton acceptor By similarity
	Binding site	269	1	ATP By similarity
Amino acid modifications
	Modified residue	389	1	Phosphotyrosine; by autocatalysis By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
Natural variations
	Natural variant	48	1	T → I		VAR_041672
	Natural variant	71	1	A → T in a colorectal adenocarcinoma sample; somatic mutation.		VAR_041673
Experimental info
	Sequence conflict	287	1	V → M in CAA83965. Ref.1
	Sequence conflict	407	1	I → Y in AAB33265. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P51451-1 [UniParc]. Last modified August 21, 2007. Version 3. Checksum: B5F739BEF8389176	FASTA	505	57,706
	10 20 30 40 50 60 MGLVSSKKPD KEKPIKEKDK GQWSPLKVSA QDKDAPPLPP LVVFNHLTPP PPDEHLDEDK 70 80 90 100 110 120 HFVVALYDYT AMNDRDLQML KGEKLQVLKG TGDWWLARSL VTGREGYVPS NFVARVESLE 130 140 150 160 170 180 MERWFFRSQG RKEAERQLLA PINKAGSFLI RESETNKGAF SLSVKDVTTQ GELIKHYKIR 190 200 210 220 230 240 CLDEGGYYIS PRITFPSLQA LVQHYSKKGD GLCQRLTLPC VRPAPQNPWA QDEWEIPRQS 250 260 270 280 290 300 LRLVRKLGSG QFGEVWMGYY KNNMKVAIKT LKEGTMSPEA FLGEANVMKA LQHERLVRLY 310 320 330 340 350 360 AVVTKEPIYI VTEYMARGCL LDFLKTDEGS RLSLPRLIDM SAQIAEGMAY IERMNSIHRD 370 380 390 400 410 420 LRAANILVSE ALCCKIADFG LARIIDSEYT AQEGAKFPIK WTAPEAIHFG VFTIKADVWS 430 440 450 460 470 480 FGVLLMEVVT YGRVPYPGMS NPEVIRNLER GYRMPRPDTC PPELYRGVIA ECWRSRPEER 490 500 PTFEFLQSVL EDFYTATERQ YELQP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, characterization, and chromosomal localization of a human lymphoid tyrosine kinase related to murine Blk." Islam K.B., Rabbani H., Larsson C., Sanders R., Smith C.I. J. Immunol. 154:1265-1272(1995) [PubMed: 7822795] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Molecular cloning and chromosomal localization of the human homologue of a B-lymphocyte specific protein tyrosine kinase (blk)." Drebin J.A., Hartzell S.W., Griffin C., Campbell M.J., Niederhuber J.E. Oncogene 10:477-486(1995) [PubMed: 7845672] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Patterns of somatic mutation in human cancer genomes." Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R. Nature 446:153-158(2007) [PubMed: 17344846] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] ILE-48; THR-71 AND THR-71.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z33998 mRNA. Translation: CAA83965.1. S76617 mRNA. Translation: AAB33265.1.
PIR	I37206.
RefSeq	NP_001706.2.
UniGene	Hs.146591
3D structure databases
HSSP	HSSP built from PDB template 1BLK based on UniProtKB P16277.
SMR	P51451. Positions 63-505.
ModBase	Search...
PTM databases
PhosphoSite	P51451.
Genome annotation databases
Ensembl	ENSG00000136573. Homo sapiens. [Contig view]
GeneID	640.
KEGG	hsa:640.
Organism-specific databases
H-InvDB	HIX0007315. HIX0058582.
HGNC	HGNC:1057. BLK.
HPA	CAB005093.
MIM	191305. gene.
PharmGKB	PA25368.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOGENOM	P51451.
HOVERGEN	P51451.
Gene expression databases
ArrayExpress	P51451.
CleanEx	HS_BLK.
GermOnline	ENSG00000136573. Homo sapiens.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR000980. SH2. IPR001452. SH3. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 1 hit. PF00017. SH2. 1 hit. PF00018. SH3_1. 1 hit. [Graphical view]
PRINTS	PR00401. SH2DOMAIN. PR00452. SH3DOMAIN. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. PD000093. SH2. 1 hit. PD000066. SH3. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00252. SH2. 1 hit. SM00326. SH3. 1 hit. SM00219. TyrKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00109. PROTEIN_KINASE_TYR. False negative. PS50001. SH2. 1 hit. PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	2596.
SOURCE	Search...

Entry information

Entry name

BLK_HUMAN

Accession

Primary (citable) accession number: P51451
Secondary accession number(s): Q16291

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	August 21, 2007
Last modified:	November 25, 2008
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)