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Protein

D(4) dopamine receptor

Gene

Drd4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Modulates the circadian rhythm of contrast sensitivity by regulating the rhythmic expression of NPAS2 in the retinal ganglion cells.1 Publication

GO - Molecular functioni

  1. dopamine binding Source: MGI
  2. dopamine neurotransmitter receptor activity Source: MGI
  3. dopamine neurotransmitter receptor activity, coupled via Gi/Go Source: MGI
  4. drug binding Source: MGI
  5. identical protein binding Source: MGI
  6. SH3 domain binding Source: MGI

GO - Biological processi

  1. activation of MAPK activity Source: MGI
  2. adenylate cyclase-inhibiting dopamine receptor signaling pathway Source: MGI
  3. adult locomotory behavior Source: MGI
  4. arachidonic acid secretion Source: MGI
  5. behavioral fear response Source: Ensembl
  6. behavioral response to cocaine Source: MGI
  7. circadian rhythm Source: Ensembl
  8. dopamine receptor signaling pathway Source: MGI
  9. fear response Source: MGI
  10. negative regulation of adenylate cyclase activity Source: MGI
  11. negative regulation of cAMP biosynthetic process Source: MGI
  12. negative regulation of protein secretion Source: MGI
  13. negative regulation of voltage-gated calcium channel activity Source: MGI
  14. olfactory learning Source: Ensembl
  15. photoperiodism Source: Ensembl
  16. positive regulation of excitatory postsynaptic membrane potential Source: Ensembl
  17. positive regulation of kinase activity Source: MGI
  18. positive regulation of penile erection Source: Ensembl
  19. positive regulation of sodium:proton antiporter activity Source: MGI
  20. regulation of calcium-mediated signaling Source: Ensembl
  21. regulation of circadian rhythm Source: UniProtKB
  22. regulation of dopamine metabolic process Source: MGI
  23. regulation of inhibitory postsynaptic membrane potential Source: MGI
  24. regulation of neurotransmitter secretion Source: Ensembl
  25. response to amphetamine Source: MGI
  26. response to histamine Source: MGI
  27. response to steroid hormone Source: Ensembl
  28. retina development in camera-type eye Source: Ensembl
  29. short-term memory Source: Ensembl
  30. synaptic transmission, dopaminergic Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Biological rhythms

Enzyme and pathway databases

ReactomeiREACT_294218. Dopamine receptors.
REACT_331048. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
D(4) dopamine receptor
Alternative name(s):
D(2C) dopamine receptor
Dopamine D4 receptor
Gene namesi
Name:Drd4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:94926. Drd4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3434ExtracellularSequence AnalysisAdd
BLAST
Transmembranei35 – 5723Helical; Name=1Sequence AnalysisAdd
BLAST
Topological domaini58 – 6710CytoplasmicSequence Analysis
Transmembranei68 – 9023Helical; Name=2Sequence AnalysisAdd
BLAST
Topological domaini91 – 10616ExtracellularSequence AnalysisAdd
BLAST
Transmembranei107 – 12822Helical; Name=3Sequence AnalysisAdd
BLAST
Topological domaini129 – 14618CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei147 – 17024Helical; Name=4Sequence AnalysisAdd
BLAST
Topological domaini171 – 18616ExtracellularSequence AnalysisAdd
BLAST
Transmembranei187 – 20822Helical; Name=5Sequence AnalysisAdd
BLAST
Topological domaini209 – 314106CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei315 – 33723Helical; Name=6Sequence AnalysisAdd
BLAST
Topological domaini338 – 3469ExtracellularSequence Analysis
Transmembranei347 – 36923Helical; Name=7Sequence AnalysisAdd
BLAST
Topological domaini370 – 38718CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. cell cortex Source: Ensembl
  2. dendritic spine Source: Ensembl
  3. integral component of plasma membrane Source: MGI
  4. membrane Source: MGI
  5. neuronal cell body Source: Ensembl
  6. terminal bouton Source: Ensembl
  7. vesicle membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Mice show a significant reduction in daytime contrast sensitivity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 387387D(4) dopamine receptorPRO_0000069402Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi3 – 31N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi105 ↔ 180PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP51436.

PTM databases

PhosphoSiteiP51436.

Expressioni

Gene expression databases

BgeeiP51436.
CleanExiMM_DRD4.
GenevestigatoriP51436.

Interactioni

Subunit structurei

Forms homo- and heterooligomers with DRD2. The interaction with DRD2 may modulate agonist-induced downstream signaling. Interacts with CLIC6 and GPRASP1. May interact with ADORA2A.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026569.

Structurei

3D structure databases

ProteinModelPortaliP51436.
SMRiP51436. Positions 32-383.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG256691.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP51436.
KOiK04147.
OMAiCGPDCAP.
OrthoDBiEOG769ZMG.
PhylomeDBiP51436.
TreeFamiTF334382.

Family and domain databases

InterProiIPR002185. Dopamine_D4_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24249:SF37. PTHR24249:SF37. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00569. DOPAMINED4R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51436-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNSSATEDG GLLAGRGPES LGTGAGLGGA GAAALVGGVL LIGLVLAGNS
60 70 80 90 100
LVCVSVASER TLQTPTNYFI VSLAAADLLL AVLVLPLFVY SEVQGGVWLL
110 120 130 140 150
SPRLCDTLMA MDVMLCTASI FNLCAISVDR FVAVTVPLRY NQQGQCQLLL
160 170 180 190 200
IAATWLLSAA VASPVVCGLN DVPGRDPAVC CLENRDYVVY SSVCSFFLPC
210 220 230 240 250
PLMLLLYWAT FRGLRRWEAA RHTKLHSRAP RRPSGPGPPV SDPTQGPFFP
260 270 280 290 300
DCPPPLPSLR TSPSDSSRPE SELSQRPCSP GCLLADAALP QPPEPSSRRR
310 320 330 340 350
RGAKITGRER KAMRVLPVVV GAFLVCWTPF FVVHITRALC PACFVSPRLV
360 370 380
SAVTWLGYVN SALNPIIYTI FNAEFRSVFR KTLRLRC
Length:387
Mass (Da):41,487
Last modified:September 30, 1996 - v1
Checksum:iBEA306D5E8AA02E9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 11M → L in BAC31893 (PubMed:16141072).Curated
Sequence conflicti19 – 191E → K in BAC31893 (PubMed:16141072).Curated
Sequence conflicti47 – 471A → T in AAB50730 (PubMed:8584230).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19880 Genomic DNA. Translation: AAC52190.1.
S80929
, S80920, S80927, S80928 Genomic DNA. Translation: AAB50730.1.
AK044379 mRNA. Translation: BAC31893.1.
CH466531 Genomic DNA. Translation: EDL18041.1.
BC016086 mRNA. Translation: AAH16086.1.
BC051421 mRNA. Translation: AAH51421.2.
CCDSiCCDS22007.1.
PIRiI49246.
RefSeqiNP_031904.1. NM_007878.2.
UniGeneiMm.41075.

Genome annotation databases

EnsembliENSMUST00000026569; ENSMUSP00000026569; ENSMUSG00000025496.
GeneIDi13491.
KEGGimmu:13491.
UCSCiuc009kkm.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U19880 Genomic DNA. Translation: AAC52190.1.
S80929
, S80920, S80927, S80928 Genomic DNA. Translation: AAB50730.1.
AK044379 mRNA. Translation: BAC31893.1.
CH466531 Genomic DNA. Translation: EDL18041.1.
BC016086 mRNA. Translation: AAH16086.1.
BC051421 mRNA. Translation: AAH51421.2.
CCDSiCCDS22007.1.
PIRiI49246.
RefSeqiNP_031904.1. NM_007878.2.
UniGeneiMm.41075.

3D structure databases

ProteinModelPortaliP51436.
SMRiP51436. Positions 32-383.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000026569.

Chemistry

ChEMBLiCHEMBL2111359.
GuidetoPHARMACOLOGYi217.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiP51436.

Proteomic databases

PRIDEiP51436.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026569; ENSMUSP00000026569; ENSMUSG00000025496.
GeneIDi13491.
KEGGimmu:13491.
UCSCiuc009kkm.1. mouse.

Organism-specific databases

CTDi1815.
MGIiMGI:94926. Drd4.

Phylogenomic databases

eggNOGiNOG256691.
GeneTreeiENSGT00760000118795.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP51436.
KOiK04147.
OMAiCGPDCAP.
OrthoDBiEOG769ZMG.
PhylomeDBiP51436.
TreeFamiTF334382.

Enzyme and pathway databases

ReactomeiREACT_294218. Dopamine receptors.
REACT_331048. G alpha (i) signalling events.

Miscellaneous databases

NextBioi284009.
PROiP51436.
SOURCEiSearch...

Gene expression databases

BgeeiP51436.
CleanExiMM_DRD4.
GenevestigatoriP51436.

Family and domain databases

InterProiIPR002185. Dopamine_D4_rcpt.
IPR000929. Dopamine_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24249:SF37. PTHR24249:SF37. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00569. DOPAMINED4R.
PR00242. DOPAMINER.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterisation of the gene encoding the murine D4 dopamine receptor."
    Fishburn C.S., Carmon S., Fuchs S.
    FEBS Lett. 361:215-219(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/Sv.
  2. "Genomic structure and tissue distribution of the mouse dopamine D4 receptor."
    Suzuki T., Kobayashi K., Nagatsu T.
    Neurosci. Lett. 199:69-72(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  4. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  6. "BTB Protein KLHL12 targets the dopamine D4 receptor for ubiquitination by a Cul3-based E3 ligase."
    Rondou P., Haegeman G., Vanhoenacker P., Van Craenenbroeck K.
    J. Biol. Chem. 283:11083-11096(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  7. "Circadian rhythm of contrast sensitivity is regulated by a dopamine-neuronal PAS-domain protein 2-adenylyl cyclase 1 signaling pathway in retinal ganglion cells."
    Hwang C.K., Chaurasia S.S., Jackson C.R., Chan G.C., Storm D.R., Iuvone P.M.
    J. Neurosci. 33:14989-14997(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiDRD4_MOUSE
AccessioniPrimary (citable) accession number: P51436
Secondary accession number(s): O35838, Q7TT80, Q8BXS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1996
Last sequence update: September 30, 1996
Last modified: March 31, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

In contrast to human protein, does not interact with KLHL12 and is not ubiquitinated by the BCR(KLHL12) complex.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.