P51436 (DRD4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
D(4) dopamine receptor

Alternative name(s):
D(2C) dopamine receptor
Dopamine D4 receptor

Gene names

Name:

Drd4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	387 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase.
Subunit structure	Forms homo- and heterooligomers with DRD2 By similarity. The interaction with DRD2 may modulate agonist-induced downstream signaling By similarity. Interacts with CLIC6 and GPRASP1 By similarity. May interact with ADORA2A By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.
Caution	In contrast to human protein, does not interact with KLHL12 and is not ubiquitinated by the BCR(KLHL12) complex (Ref.6).

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of MAPK activity Inferred from electronic annotation. Source: Compara adenylate cyclase-inhibiting dopamine receptor signaling pathway Inferred from mutant phenotype PubMed 14684868. Source: MGI adult locomotory behavior Inferred from mutant phenotype PubMed 9323127. Source: MGI arachidonic acid secretion Inferred from electronic annotation. Source: Compara behavioral fear response Inferred from electronic annotation. Source: Compara behavioral response to cocaine Inferred from mutant phenotype PubMed 12783155PubMed 9323127. Source: MGI fear response Inferred from mutant phenotype PubMed 11860516. Source: MGI negative regulation of adenylate cyclase activity Inferred from electronic annotation. Source: Compara negative regulation of protein secretion Inferred from electronic annotation. Source: Compara negative regulation of voltage-gated calcium channel activity Inferred from electronic annotation. Source: Compara olfactory learning Inferred from electronic annotation. Source: Compara positive regulation of sodium:hydrogen antiporter activity Inferred from electronic annotation. Source: Compara regulation of calcium-mediated signaling Inferred from electronic annotation. Source: Compara regulation of dopamine metabolic process Inferred from mutant phenotype PubMed 9323127. Source: MGI regulation of inhibitory postsynaptic membrane potential Inferred from mutant phenotype PubMed 11356863PubMed 14684868. Source: MGI regulation of neurotransmitter secretion Inferred from electronic annotation. Source: Compara response to amphetamine Inferred from mutant phenotype PubMed 9323127. Source: MGI response to histamine Inferred from electronic annotation. Source: Compara short-term memory Inferred from electronic annotation. Source: Compara synaptic transmission, dopaminergic Inferred from electronic annotation. Source: Compara
Cellular_component	cell cortex Inferred from electronic annotation. Source: Compara dendritic spine Inferred from electronic annotation. Source: Compara integral to plasma membrane Inferred from electronic annotation. Source: Compara neuronal cell body Inferred from electronic annotation. Source: Compara terminal bouton Inferred from electronic annotation. Source: Compara vesicle membrane Inferred from electronic annotation. Source: Compara
Molecular_function	dopamine binding Inferred from electronic annotation. Source: Compara dopamine neurotransmitter receptor activity, coupled via Gi/Go Inferred from mutant phenotype PubMed 11356863. Source: MGI drug binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 387	387	D(4) dopamine receptor		PRO_0000069402
Regions
	Topological domain	1 – 34	34	Extracellular Potential
	Transmembrane	35 – 57	23	Helical; Name=1; Potential
	Topological domain	58 – 67	10	Cytoplasmic Potential
	Transmembrane	68 – 90	23	Helical; Name=2; Potential
	Topological domain	91 – 106	16	Extracellular Potential
	Transmembrane	107 – 128	22	Helical; Name=3; Potential
	Topological domain	129 – 146	18	Cytoplasmic Potential
	Transmembrane	147 – 170	24	Helical; Name=4; Potential
	Topological domain	171 – 186	16	Extracellular Potential
	Transmembrane	187 – 208	22	Helical; Name=5; Potential
	Topological domain	209 – 314	106	Cytoplasmic Potential
	Transmembrane	315 – 337	23	Helical; Name=6; Potential
	Topological domain	338 – 346	9	Extracellular Potential
	Transmembrane	347 – 369	23	Helical; Name=7; Potential
	Topological domain	370 – 387	18	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	3	1	N-linked (GlcNAc...) Potential
	Disulfide bond	105 ↔ 180		By similarity
Experimental info
	Sequence conflict	1	1	M → L in BAC31893. Ref.3
	Sequence conflict	19	1	E → K in BAC31893. Ref.3
	Sequence conflict	47	1	A → T in AAB50730. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P51436 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BEA306D5E8AA02E9	FASTA	387	41,487
	10 20 30 40 50 60 MGNSSATEDG GLLAGRGPES LGTGAGLGGA GAAALVGGVL LIGLVLAGNS LVCVSVASER 70 80 90 100 110 120 TLQTPTNYFI VSLAAADLLL AVLVLPLFVY SEVQGGVWLL SPRLCDTLMA MDVMLCTASI 130 140 150 160 170 180 FNLCAISVDR FVAVTVPLRY NQQGQCQLLL IAATWLLSAA VASPVVCGLN DVPGRDPAVC 190 200 210 220 230 240 CLENRDYVVY SSVCSFFLPC PLMLLLYWAT FRGLRRWEAA RHTKLHSRAP RRPSGPGPPV 250 260 270 280 290 300 SDPTQGPFFP DCPPPLPSLR TSPSDSSRPE SELSQRPCSP GCLLADAALP QPPEPSSRRR 310 320 330 340 350 360 RGAKITGRER KAMRVLPVVV GAFLVCWTPF FVVHITRALC PACFVSPRLV SAVTWLGYVN 370 380 SALNPIIYTI FNAEFRSVFR KTLRLRC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterisation of the gene encoding the murine D4 dopamine receptor." Fishburn C.S., Carmon S., Fuchs S. FEBS Lett. 361:215-219(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[2]	"Genomic structure and tissue distribution of the mouse dopamine D4 receptor." Suzuki T., Kobayashi K., Nagatsu T. Neurosci. Lett. 199:69-72(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Retina.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Retina.
[6]	"BTB Protein KLHL12 targets the dopamine D4 receptor for ubiquitination by a Cul3-based E3 ligase." Rondou P., Haegeman G., Vanhoenacker P., Van Craenenbroeck K. J. Biol. Chem. 283:11083-11096(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19880 Genomic DNA. Translation: AAC52190.1. S80929 , S80920, S80927, S80928 Genomic DNA. Translation: AAB50730.1. AK044379 mRNA. Translation: BAC31893.1. CH466531 Genomic DNA. Translation: EDL18041.1. BC016086 mRNA. Translation: AAH16086.1. BC051421 mRNA. Translation: AAH51421.2.
IPI	IPI00122474.
PIR	I49246.
RefSeq	NP_031904.1. NM_007878.2.
UniGene	Mm.41075.
3D structure databases
ProteinModelPortal	P51436.
SMR	P51436. Positions 29-386.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000026569.
Protein family/group databases
GPCRDB	Search...
PTM databases
PhosphoSite	P51436.
Proteomic databases
PRIDE	P51436.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000026569; ENSMUSP00000026569; ENSMUSG00000025496.
GeneID	13491.
KEGG	mmu:13491.
Organism-specific databases
CTD	1815.
MGI	MGI:94926. Drd4.
Phylogenomic databases
eggNOG	NOG256691.
GeneTree	ENSGT00700000104198.
HOGENOM	HOG000239242.
HOVERGEN	HBG106962.
InParanoid	Q7TT80.
KO	K04147.
OMA	VQGGAWL.
OrthoDB	EOG4PNXHQ.
Gene expression databases
Bgee	P51436.
CleanEx	MM_DRD4.
Genevestigator	P51436.
GermOnline	ENSMUSG00000025496. Mus musculus.
Family and domain databases
InterPro	IPR002185. Dopa_D4_rcpt. IPR000929. Dopamine_rcpt. IPR000276. GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_7TM. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00569. DOPAMINED4R. PR00242. DOPAMINER. PR00237. GPCRRHODOPSN.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P51436.
ChEMBL	CHEMBL2574.
NextBio	284009.
SOURCE	Search...

Entry information

Entry name

DRD4_MOUSE

Accession

Primary (citable) accession number: P51436
Secondary accession number(s): O35838, Q7TT80, Q8BXS4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program