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Protein

Calcium-dependent phospholipase A2

Gene

Pla2g5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes L-alpha-palmitoyl-2-oleoyl phosphatidylcholine more efficiently than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Calcium; via carbonyl oxygenBy similarity
Metal bindingi49 – 491Calcium; via carbonyl oxygenBy similarity
Metal bindingi51 – 511Calcium; via carbonyl oxygenBy similarity
Active sitei67 – 671By similarity
Metal bindingi68 – 681CalciumBy similarity
Active sitei111 – 1111By similarity

GO - Molecular functioni

  • calcium-dependent phospholipase A2 activity Source: RGD
  • calcium ion binding Source: InterPro
  • heparin binding Source: RGD

GO - Biological processi

  • arachidonic acid secretion Source: RGD
  • leukotriene biosynthetic process Source: RGD
  • lipid catabolic process Source: UniProtKB-KW
  • platelet activating factor biosynthetic process Source: RGD
  • positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  • positive regulation of phospholipase activity Source: Ensembl
  • response to cAMP Source: RGD
  • response to cytokine Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-RNO-1482788. Acyl chain remodelling of PC.
R-RNO-1482801. Acyl chain remodelling of PS.
R-RNO-1482839. Acyl chain remodelling of PE.
R-RNO-1482922. Acyl chain remodelling of PI.
R-RNO-1482925. Acyl chain remodelling of PG.
R-RNO-1483166. Synthesis of PA.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Group V phospholipase A2
PLA2-10
Phosphatidylcholine 2-acylhydrolase 5
Gene namesi
Name:Pla2g5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi62051. Pla2g5.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB-SubCell
  • Golgi apparatus Source: RGD
  • perinuclear region of cytoplasm Source: RGD
  • plasma membrane Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 137117Calcium-dependent phospholipase A2PRO_0000022763Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 137By similarity
Disulfide bondi48 ↔ 64By similarity
Disulfide bondi63 ↔ 117By similarity
Disulfide bondi70 ↔ 110By similarity
Disulfide bondi79 ↔ 103By similarity
Disulfide bondi97 ↔ 108By similarity

Post-translational modificationi

This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP51433.

Expressioni

Gene expression databases

GenevisibleiP51433. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022715.

Structurei

3D structure databases

ProteinModelPortaliP51433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4087. Eukaryota.
ENOG411283D. LUCA.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiP51433.
KOiK01047.
OMAiYCLKRNL.
OrthoDBiEOG7N63PF.
PhylomeDBiP51433.
TreeFamiTF319283.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRLLTLAWF LACSVPAVPG GLLELKSMIE KVTGKNAVKN YGFYGCYCGW
60 70 80 90 100
GGHGTPKDGT DWCCRMHDRC YGLLEEKHCA IRTQSYDYRF TQDLVICEHD
110 120 130
SFCPVRLCAC DRKLVYCLRR NLWSYNRLYQ YYPNFLC
Length:137
Mass (Da):15,996
Last modified:October 1, 1996 - v1
Checksum:iB21607EF741FA608
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03763 mRNA. Translation: AAA82112.1.
AY651028 mRNA. Translation: AAT68714.1.
BC085745 mRNA. Translation: AAH85745.1.
RefSeqiNP_058870.1. NM_017174.1.
XP_006239201.1. XM_006239139.2.
XP_006239202.1. XM_006239140.2.
XP_006239203.1. XM_006239141.2.
XP_008762431.1. XM_008764209.1.
XP_008762432.1. XM_008764210.1.
UniGeneiRn.20244.

Genome annotation databases

EnsembliENSRNOT00000022716; ENSRNOP00000022715; ENSRNOG00000016838.
GeneIDi29354.
KEGGirno:29354.
UCSCiRGD:62051. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U03763 mRNA. Translation: AAA82112.1.
AY651028 mRNA. Translation: AAT68714.1.
BC085745 mRNA. Translation: AAH85745.1.
RefSeqiNP_058870.1. NM_017174.1.
XP_006239201.1. XM_006239139.2.
XP_006239202.1. XM_006239140.2.
XP_006239203.1. XM_006239141.2.
XP_008762431.1. XM_008764209.1.
XP_008762432.1. XM_008764210.1.
UniGeneiRn.20244.

3D structure databases

ProteinModelPortaliP51433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000022715.

Proteomic databases

PaxDbiP51433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022716; ENSRNOP00000022715; ENSRNOG00000016838.
GeneIDi29354.
KEGGirno:29354.
UCSCiRGD:62051. rat.

Organism-specific databases

CTDi5322.
RGDi62051. Pla2g5.

Phylogenomic databases

eggNOGiKOG4087. Eukaryota.
ENOG411283D. LUCA.
GeneTreeiENSGT00760000119160.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiP51433.
KOiK01047.
OMAiYCLKRNL.
OrthoDBiEOG7N63PF.
PhylomeDBiP51433.
TreeFamiTF319283.

Enzyme and pathway databases

ReactomeiR-RNO-1482788. Acyl chain remodelling of PC.
R-RNO-1482801. Acyl chain remodelling of PS.
R-RNO-1482839. Acyl chain remodelling of PE.
R-RNO-1482922. Acyl chain remodelling of PI.
R-RNO-1482925. Acyl chain remodelling of PG.
R-RNO-1483166. Synthesis of PA.

Miscellaneous databases

PROiP51433.

Gene expression databases

GenevisibleiP51433. RN.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression and partial characterization of a novel rat phospholipase A2."
    Chen J., Engle S.J., Seilhamer J.J., Tischfield J.A.
    Biochim. Biophys. Acta 1215:115-120(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and sequence determination of rat group V phospholipase A2 from ovary."
    Liang N.S., Su Q.B., Li Y., Yang F., Lu Y., Xie Y.A.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Ovary.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.

Entry informationi

Entry nameiPA2G5_RAT
AccessioniPrimary (citable) accession number: P51433
Secondary accession number(s): Q6DQ96
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.