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P51432

- PLCB3_MOUSE

UniProt

P51432 - PLCB3_MOUSE

Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Gene

Plcb3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

    Catalytic activityi

    1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

    Cofactori

    Calcium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei332 – 3321PROSITE-ProRule annotation
    Active sitei379 – 3791PROSITE-ProRule annotation

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. phosphatidylinositol phospholipase C activity Source: UniProtKB-EC
    3. signal transducer activity Source: UniProtKB-KW

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. lipid catabolic process Source: UniProtKB-KW
    3. regulation of systemic arterial blood pressure Source: MGI
    4. synaptic transmission Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Transducer

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_196473. Synthesis of IP3 and IP4 in the cytosol.
    REACT_198612. Presynaptic function of Kainate receptors.
    REACT_207651. G alpha (q) signalling events.
    REACT_221970. Ca2+ pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (EC:3.1.4.11)
    Alternative name(s):
    Phosphoinositide phospholipase C-beta-3
    Phospholipase C-beta-3
    Short name:
    PLC-beta-3
    Gene namesi
    Name:Plcb3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:104778. Plcb3.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell
    2. protein complex Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 123412331-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3PRO_0000088492Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei474 – 4741PhosphoserineBy similarity
    Modified residuei537 – 5371PhosphoserineBy similarity
    Modified residuei1105 – 11051PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP51432.
    PaxDbiP51432.
    PRIDEiP51432.

    PTM databases

    PhosphoSiteiP51432.

    Expressioni

    Tissue specificityi

    Highly expressed in kidney, skeletal muscle, liver, lung, heart and brain.

    Gene expression databases

    ArrayExpressiP51432.
    BgeeiP51432.
    CleanExiMM_PLCB3.
    GenevestigatoriP51432.

    Interactioni

    Subunit structurei

    Interacts with SHANK2 and LPAR2.By similarity

    Protein-protein interaction databases

    MINTiMINT-126696.

    Structurei

    3D structure databases

    ProteinModelPortaliP51432.
    SMRiP51432. Positions 10-505, 541-882, 935-1192.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini318 – 468151PI-PLC X-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini591 – 707117PI-PLC Y-boxPROSITE-ProRule annotationAdd
    BLAST
    Domaini714 – 81198C2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1231 – 12344Interaction with SHANK2By similarity

    Sequence similaritiesi

    Contains 1 C2 domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
    Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG149692.
    GeneTreeiENSGT00730000110266.
    HOGENOMiHOG000232046.
    HOVERGENiHBG053609.
    InParanoidiQ3UIS0.
    OMAiEFQNRQV.
    OrthoDBiEOG7WDN1N.
    TreeFamiTF313216.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProiIPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028390. PLC-beta3.
    IPR014815. PLC-beta_C.
    IPR009535. PLC-beta_CS.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view]
    PANTHERiPTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF11. PTHR10336:SF11. 1 hit.
    PfamiPF00168. C2. 1 hit.
    PF06631. DUF1154. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000956. PLC-beta. 1 hit.
    PRINTSiPR00390. PHPHLIPASEC.
    SMARTiSM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEiPS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P51432-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGARPGVHA LQLEPPTVVE TLRRGSKFIK WDEEASSRNL VTLRVDPNGF     50
    FLYWTGPNME VDTLDISSIR DTRTGRYARL PKDPKIREVL GFGGPDTRLE 100
    EKLMTVVAGP DPVNTTFLNF MAVQDDTVKV WSEELFKLAM NILAQNASRN 150
    TFLRKAYTKL KLQVNQDGRI PVKNILKMFS ADKKRVETAL ESCGLNFNRS 200
    ESIRPDEFPL EIFERFLNKL CLRPDIDKIL LEIGAKGKPY LTLEQLMDFI 250
    NQKQRDPRLN EVLYPPLRSS QARLLIEKYE TNKQFLERDQ MSMEGFSRYL 300
    GGEENGILPL EALDLSMDMT QPLSAYFINS SHNTYLTAGQ LAGPSSVEMY 350
    RQALLWGCRC VELDVWKGRP PEEEPFITHG FTMTTEVPLR DVLEAIAEAA 400
    FKTSPYPVIL SFENHVDSAK QQAKMAEYCR SIFGDALLID PLDKYPLSAG 450
    IPLPSPQDLM GRILVKNKKR HRPSTGVPDS SVRKRPLEQS NSALSESSAA 500
    TEPSSPQLGS PSSDSCPGLS NGEEVGLEKT SLEPQKSLGE ESLSREPNVP 550
    MPDRDREDEE EDEEEEETTD PKKPTTDEGT ASSEVNATEE MSTLVNYVEP 600
    VKFKSFEAAR KRNKCFEMSS FVETKAMEQL TKSPMEFVEY NKQQLSRIYP 650
    KGTRVDSSNY MPQLFWNVGC QLVALNFQTL DLPMQLNAGV FEYNGRSGYL 700
    LKPEFMRRPD KSFDPFTEVI VDGIVANALR VKVISGQFLS DKKVGIYVEV 750
    DMFGLPVDTR RKYRTRTSQG NSFNPVWDEE PFDFPKVVLP TLASLRIAAF 800
    EEGGKFVGHR ILPVSAIRSG YHYVCLRNEA NQPLCLPALL IYTEASDYIP 850
    DDHQDYAEAL INPIKHVSLM DQRAKQLAAL IGESEAQAST ETYQETPCQQ 900
    PGSQLPSNPT PNPLDASPRW PPGPTTSSTS SSLSSPGQRD DLIASILSEV 950
    TPTPLEELRS HKAMVKLRSR QDRDLRELHK KHQRKAVALT RRLLDGLAQA 1000
    RAEGKCRPSP SALGKATNSE DVKEEEEAKQ YREFQNRQVQ SLLELREAQA 1050
    DVETKRKLEH LRQAHQRLKE VVLDTHTTQF KRLKELNERE KKELQKILDR 1100
    KRNNSISEAK TREKHKKEVE LTEINRRHIT ESVNSIRRLE EAQKQRHERL 1150
    VAGQQQVLQQ LEEEEPKLLA QLTQECQEQR ERLPQEIRRC LLGETAEGLG 1200
    DGPLVACASN GHAPGSGGHL SSADSESQEE NTQL 1234
    Length:1,234
    Mass (Da):139,487
    Last modified:July 27, 2011 - v2
    Checksum:iE055744A33C5F6B0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 161P → S in AAA85199. (PubMed:9714794)Curated
    Sequence conflicti20 – 201E → K in AAA85199. (PubMed:9714794)Curated
    Sequence conflicti609 – 6091A → S in AAA85199. (PubMed:9714794)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43144 mRNA. Translation: AAA85199.1.
    AK146793 mRNA. Translation: BAE27436.1.
    CH466612 Genomic DNA. Translation: EDL33267.1.
    CCDSiCCDS70925.1.
    RefSeqiNP_001277278.1. NM_001290349.1.
    NP_032900.2. NM_008874.4.
    UniGeneiMm.273204.

    Genome annotation databases

    EnsembliENSMUST00000025912; ENSMUSP00000025912; ENSMUSG00000024960.
    GeneIDi18797.
    UCSCiuc008gjr.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U43144 mRNA. Translation: AAA85199.1 .
    AK146793 mRNA. Translation: BAE27436.1 .
    CH466612 Genomic DNA. Translation: EDL33267.1 .
    CCDSi CCDS70925.1.
    RefSeqi NP_001277278.1. NM_001290349.1.
    NP_032900.2. NM_008874.4.
    UniGenei Mm.273204.

    3D structure databases

    ProteinModelPortali P51432.
    SMRi P51432. Positions 10-505, 541-882, 935-1192.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-126696.

    PTM databases

    PhosphoSitei P51432.

    Proteomic databases

    MaxQBi P51432.
    PaxDbi P51432.
    PRIDEi P51432.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025912 ; ENSMUSP00000025912 ; ENSMUSG00000024960 .
    GeneIDi 18797.
    UCSCi uc008gjr.1. mouse.

    Organism-specific databases

    MGIi MGI:104778. Plcb3.

    Phylogenomic databases

    eggNOGi NOG149692.
    GeneTreei ENSGT00730000110266.
    HOGENOMi HOG000232046.
    HOVERGENi HBG053609.
    InParanoidi Q3UIS0.
    OMAi EFQNRQV.
    OrthoDBi EOG7WDN1N.
    TreeFami TF313216.

    Enzyme and pathway databases

    Reactomei REACT_196473. Synthesis of IP3 and IP4 in the cytosol.
    REACT_198612. Presynaptic function of Kainate receptors.
    REACT_207651. G alpha (q) signalling events.
    REACT_221970. Ca2+ pathway.

    Miscellaneous databases

    ChiTaRSi PLCB3. mouse.
    PROi P51432.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P51432.
    Bgeei P51432.
    CleanExi MM_PLCB3.
    Genevestigatori P51432.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    2.60.40.150. 1 hit.
    3.20.20.190. 2 hits.
    InterProi IPR000008. C2_dom.
    IPR011992. EF-hand-dom_pair.
    IPR001192. PI-PLC_fam.
    IPR016280. PLC-beta.
    IPR028390. PLC-beta3.
    IPR014815. PLC-beta_C.
    IPR009535. PLC-beta_CS.
    IPR017946. PLC-like_Pdiesterase_TIM-brl.
    IPR015359. PLipase_C_EF-hand-like.
    IPR000909. PLipase_C_PInositol-sp_X_dom.
    IPR001711. PLipase_C_Pinositol-sp_Y.
    [Graphical view ]
    PANTHERi PTHR10336. PTHR10336. 1 hit.
    PTHR10336:SF11. PTHR10336:SF11. 1 hit.
    Pfami PF00168. C2. 1 hit.
    PF06631. DUF1154. 1 hit.
    PF09279. EF-hand_like. 1 hit.
    PF00388. PI-PLC-X. 1 hit.
    PF00387. PI-PLC-Y. 1 hit.
    PF08703. PLC-beta_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000956. PLC-beta. 1 hit.
    PRINTSi PR00390. PHPHLIPASEC.
    SMARTi SM00239. C2. 1 hit.
    SM00148. PLCXc. 1 hit.
    SM00149. PLCYc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49562. SSF49562. 1 hit.
    SSF51695. SSF51695. 2 hits.
    PROSITEi PS50004. C2. 1 hit.
    PS50007. PIPLC_X_DOMAIN. 1 hit.
    PS50008. PIPLC_Y_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a cDNA encoding mouse phospholipase C-beta3."
      Wang S., Zhou Y., Lukinius A., Oberg K., Skogseid B., Gobl A.
      Biochim. Biophys. Acta 1393:173-178(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
      Strain: BALB/c.
      Tissue: Kidney.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiPLCB3_MOUSE
    AccessioniPrimary (citable) accession number: P51432
    Secondary accession number(s): Q3UIS0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 128 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3