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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Gene

Plcb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332PROSITE-ProRule annotation1
Active sitei379PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Transducer

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-MMU-399997. Acetylcholine regulates insulin secretion.
R-MMU-4086398. Ca2+ pathway.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-418217. G beta:gamma signalling through PLC beta.
R-MMU-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-MMU-500657. Presynaptic function of Kainate receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-3
Phospholipase C-beta-3
Short name:
PLC-beta-3
Gene namesi
Name:Plcb3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:104778. Plcb3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000884922 – 12341-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3Add BLAST1233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei474PhosphoserineBy similarity1
Modified residuei490PhosphoserineBy similarity1
Modified residuei495PhosphoserineBy similarity1
Modified residuei537PhosphoserineCombined sources1
Modified residuei927PhosphoserineBy similarity1
Modified residuei1105PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP51432.
MaxQBiP51432.
PaxDbiP51432.
PeptideAtlasiP51432.
PRIDEiP51432.

PTM databases

iPTMnetiP51432.
PhosphoSitePlusiP51432.

Expressioni

Tissue specificityi

Highly expressed in kidney, skeletal muscle, liver, lung, heart and brain.

Gene expression databases

BgeeiENSMUSG00000024960.
CleanExiMM_PLCB3.
GenevisibleiP51432. MM.

Interactioni

Subunit structurei

Interacts with SHANK2 and LPAR2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

MINTiMINT-126696.
STRINGi10090.ENSMUSP00000025912.

Structurei

3D structure databases

ProteinModelPortaliP51432.
SMRiP51432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini318 – 468PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST151
Domaini591 – 707PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini714 – 811C2PROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1231 – 1234Interaction with SHANK2By similarity4

Sequence similaritiesi

Contains 1 C2 domain.PROSITE-ProRule annotation
Contains 1 PI-PLC X-box domain.PROSITE-ProRule annotation
Contains 1 PI-PLC Y-box domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiP51432.
KOiK05858.
OMAiVDSNGFF.
OrthoDBiEOG091G00XL.
TreeFamiTF313216.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR011993. PH_dom-like.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028390. PLC-beta3.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF11. PTHR10336:SF11. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGARPGVHA LQLEPPTVVE TLRRGSKFIK WDEEASSRNL VTLRVDPNGF
60 70 80 90 100
FLYWTGPNME VDTLDISSIR DTRTGRYARL PKDPKIREVL GFGGPDTRLE
110 120 130 140 150
EKLMTVVAGP DPVNTTFLNF MAVQDDTVKV WSEELFKLAM NILAQNASRN
160 170 180 190 200
TFLRKAYTKL KLQVNQDGRI PVKNILKMFS ADKKRVETAL ESCGLNFNRS
210 220 230 240 250
ESIRPDEFPL EIFERFLNKL CLRPDIDKIL LEIGAKGKPY LTLEQLMDFI
260 270 280 290 300
NQKQRDPRLN EVLYPPLRSS QARLLIEKYE TNKQFLERDQ MSMEGFSRYL
310 320 330 340 350
GGEENGILPL EALDLSMDMT QPLSAYFINS SHNTYLTAGQ LAGPSSVEMY
360 370 380 390 400
RQALLWGCRC VELDVWKGRP PEEEPFITHG FTMTTEVPLR DVLEAIAEAA
410 420 430 440 450
FKTSPYPVIL SFENHVDSAK QQAKMAEYCR SIFGDALLID PLDKYPLSAG
460 470 480 490 500
IPLPSPQDLM GRILVKNKKR HRPSTGVPDS SVRKRPLEQS NSALSESSAA
510 520 530 540 550
TEPSSPQLGS PSSDSCPGLS NGEEVGLEKT SLEPQKSLGE ESLSREPNVP
560 570 580 590 600
MPDRDREDEE EDEEEEETTD PKKPTTDEGT ASSEVNATEE MSTLVNYVEP
610 620 630 640 650
VKFKSFEAAR KRNKCFEMSS FVETKAMEQL TKSPMEFVEY NKQQLSRIYP
660 670 680 690 700
KGTRVDSSNY MPQLFWNVGC QLVALNFQTL DLPMQLNAGV FEYNGRSGYL
710 720 730 740 750
LKPEFMRRPD KSFDPFTEVI VDGIVANALR VKVISGQFLS DKKVGIYVEV
760 770 780 790 800
DMFGLPVDTR RKYRTRTSQG NSFNPVWDEE PFDFPKVVLP TLASLRIAAF
810 820 830 840 850
EEGGKFVGHR ILPVSAIRSG YHYVCLRNEA NQPLCLPALL IYTEASDYIP
860 870 880 890 900
DDHQDYAEAL INPIKHVSLM DQRAKQLAAL IGESEAQAST ETYQETPCQQ
910 920 930 940 950
PGSQLPSNPT PNPLDASPRW PPGPTTSSTS SSLSSPGQRD DLIASILSEV
960 970 980 990 1000
TPTPLEELRS HKAMVKLRSR QDRDLRELHK KHQRKAVALT RRLLDGLAQA
1010 1020 1030 1040 1050
RAEGKCRPSP SALGKATNSE DVKEEEEAKQ YREFQNRQVQ SLLELREAQA
1060 1070 1080 1090 1100
DVETKRKLEH LRQAHQRLKE VVLDTHTTQF KRLKELNERE KKELQKILDR
1110 1120 1130 1140 1150
KRNNSISEAK TREKHKKEVE LTEINRRHIT ESVNSIRRLE EAQKQRHERL
1160 1170 1180 1190 1200
VAGQQQVLQQ LEEEEPKLLA QLTQECQEQR ERLPQEIRRC LLGETAEGLG
1210 1220 1230
DGPLVACASN GHAPGSGGHL SSADSESQEE NTQL
Length:1,234
Mass (Da):139,487
Last modified:July 27, 2011 - v2
Checksum:iE055744A33C5F6B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16P → S in AAA85199 (PubMed:9714794).Curated1
Sequence conflicti20E → K in AAA85199 (PubMed:9714794).Curated1
Sequence conflicti609A → S in AAA85199 (PubMed:9714794).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43144 mRNA. Translation: AAA85199.1.
AK146793 mRNA. Translation: BAE27436.1.
CH466612 Genomic DNA. Translation: EDL33267.1.
CCDSiCCDS70925.1.
RefSeqiNP_001277278.1. NM_001290349.1.
NP_032900.2. NM_008874.4.
UniGeneiMm.273204.

Genome annotation databases

EnsembliENSMUST00000025912; ENSMUSP00000025912; ENSMUSG00000024960.
GeneIDi18797.
KEGGimmu:18797.
UCSCiuc008gjr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43144 mRNA. Translation: AAA85199.1.
AK146793 mRNA. Translation: BAE27436.1.
CH466612 Genomic DNA. Translation: EDL33267.1.
CCDSiCCDS70925.1.
RefSeqiNP_001277278.1. NM_001290349.1.
NP_032900.2. NM_008874.4.
UniGeneiMm.273204.

3D structure databases

ProteinModelPortaliP51432.
SMRiP51432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-126696.
STRINGi10090.ENSMUSP00000025912.

PTM databases

iPTMnetiP51432.
PhosphoSitePlusiP51432.

Proteomic databases

EPDiP51432.
MaxQBiP51432.
PaxDbiP51432.
PeptideAtlasiP51432.
PRIDEiP51432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025912; ENSMUSP00000025912; ENSMUSG00000024960.
GeneIDi18797.
KEGGimmu:18797.
UCSCiuc008gjr.2. mouse.

Organism-specific databases

CTDi5331.
MGIiMGI:104778. Plcb3.

Phylogenomic databases

eggNOGiKOG0169. Eukaryota.
ENOG410XPSW. LUCA.
GeneTreeiENSGT00760000118936.
HOGENOMiHOG000232046.
HOVERGENiHBG053609.
InParanoidiP51432.
KOiK05858.
OMAiVDSNGFF.
OrthoDBiEOG091G00XL.
TreeFamiTF313216.

Enzyme and pathway databases

ReactomeiR-MMU-112043. PLC beta mediated events.
R-MMU-1855204. Synthesis of IP3 and IP4 in the cytosol.
R-MMU-399997. Acetylcholine regulates insulin secretion.
R-MMU-4086398. Ca2+ pathway.
R-MMU-416476. G alpha (q) signalling events.
R-MMU-418217. G beta:gamma signalling through PLC beta.
R-MMU-434316. Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion.
R-MMU-500657. Presynaptic function of Kainate receptors.

Miscellaneous databases

ChiTaRSiPlcb3. mouse.
PROiP51432.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024960.
CleanExiMM_PLCB3.
GenevisibleiP51432. MM.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
2.60.40.150. 1 hit.
3.20.20.190. 2 hits.
InterProiIPR000008. C2_dom.
IPR011992. EF-hand-dom_pair.
IPR011993. PH_dom-like.
IPR001192. PI-PLC_fam.
IPR016280. PLC-beta.
IPR028390. PLC-beta3.
IPR014815. PLC-beta_C.
IPR017946. PLC-like_Pdiesterase_TIM-brl.
IPR015359. PLC_EF-hand-like.
IPR000909. PLipase_C_PInositol-sp_X_dom.
IPR001711. PLipase_C_Pinositol-sp_Y.
[Graphical view]
PANTHERiPTHR10336. PTHR10336. 1 hit.
PTHR10336:SF11. PTHR10336:SF11. 1 hit.
PfamiPF00168. C2. 1 hit.
PF09279. EF-hand_like. 1 hit.
PF00388. PI-PLC-X. 1 hit.
PF00387. PI-PLC-Y. 1 hit.
PF08703. PLC-beta_C. 1 hit.
[Graphical view]
PIRSFiPIRSF000956. PLC-beta. 1 hit.
PRINTSiPR00390. PHPHLIPASEC.
SMARTiSM00239. C2. 1 hit.
SM00148. PLCXc. 1 hit.
SM00149. PLCYc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF51695. SSF51695. 2 hits.
PROSITEiPS50004. C2. 1 hit.
PS50007. PIPLC_X_DOMAIN. 1 hit.
PS50008. PIPLC_Y_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLCB3_MOUSE
AccessioniPrimary (citable) accession number: P51432
Secondary accession number(s): Q3UIS0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 149 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.