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Protein

1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3

Gene

Plcb3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.

Catalytic activityi

1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol.

Cofactori

Ca2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei332PROSITE-ProRule annotation1
Active sitei379PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Transducer
Biological processLipid degradation, Lipid metabolism
LigandCalcium

Enzyme and pathway databases

ReactomeiR-MMU-112043 PLC beta mediated events
R-MMU-1855204 Synthesis of IP3 and IP4 in the cytosol
R-MMU-399997 Acetylcholine regulates insulin secretion
R-MMU-4086398 Ca2+ pathway
R-MMU-416476 G alpha (q) signalling events
R-MMU-418217 G beta:gamma signalling through PLC beta
R-MMU-434316 Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion
R-MMU-500657 Presynaptic function of Kainate receptors

Names & Taxonomyi

Protein namesi
Recommended name:
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3 (EC:3.1.4.11)
Alternative name(s):
Phosphoinositide phospholipase C-beta-3
Phospholipase C-beta-3
Short name:
PLC-beta-3
Gene namesi
Name:Plcb3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:104778 Plcb3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000884922 – 12341-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-3Add BLAST1233

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei474PhosphoserineBy similarity1
Modified residuei490PhosphoserineBy similarity1
Modified residuei495PhosphoserineBy similarity1
Modified residuei537PhosphoserineCombined sources1
Modified residuei927PhosphoserineBy similarity1
Modified residuei1105PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP51432
MaxQBiP51432
PaxDbiP51432
PeptideAtlasiP51432
PRIDEiP51432

PTM databases

iPTMnetiP51432
PhosphoSitePlusiP51432

Expressioni

Tissue specificityi

Highly expressed in kidney, skeletal muscle, liver, lung, heart and brain.

Gene expression databases

BgeeiENSMUSG00000024960
CleanExiMM_PLCB3
GenevisibleiP51432 MM

Interactioni

Subunit structurei

Interacts with SHANK2 and LPAR2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

MINTiP51432
STRINGi10090.ENSMUSP00000025912

Structurei

3D structure databases

ProteinModelPortaliP51432
SMRiP51432
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini318 – 468PI-PLC X-boxPROSITE-ProRule annotationAdd BLAST151
Domaini591 – 707PI-PLC Y-boxPROSITE-ProRule annotationAdd BLAST117
Domaini714 – 811C2PROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1231 – 1234Interaction with SHANK2By similarity4

Phylogenomic databases

eggNOGiKOG0169 Eukaryota
ENOG410XPSW LUCA
GeneTreeiENSGT00760000118936
HOGENOMiHOG000232046
HOVERGENiHBG053609
InParanoidiP51432
KOiK05858
OMAiQECQEQR
OrthoDBiEOG091G00XL
TreeFamiTF313216

Family and domain databases

CDDicd13361 PH_PLC_beta, 1 hit
Gene3Di2.60.40.150, 1 hit
3.20.20.190, 2 hits
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR011992 EF-hand-dom_pair
IPR001192 PI-PLC_fam
IPR016280 PLC-beta
IPR014815 PLC-beta_C
IPR037862 PLC-beta_PH
IPR017946 PLC-like_Pdiesterase_TIM-brl
IPR015359 PLC_EF-hand-like
IPR000909 PLipase_C_PInositol-sp_X_dom
IPR001711 PLipase_C_Pinositol-sp_Y
PANTHERiPTHR10336 PTHR10336, 1 hit
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF09279 EF-hand_like, 1 hit
PF00388 PI-PLC-X, 1 hit
PF00387 PI-PLC-Y, 1 hit
PF08703 PLC-beta_C, 1 hit
PIRSFiPIRSF000956 PLC-beta, 1 hit
PRINTSiPR00390 PHPHLIPASEC
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00148 PLCXc, 1 hit
SM00149 PLCYc, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
SSF51695 SSF51695, 2 hits
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50007 PIPLC_X_DOMAIN, 1 hit
PS50008 PIPLC_Y_DOMAIN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGARPGVHA LQLEPPTVVE TLRRGSKFIK WDEEASSRNL VTLRVDPNGF
60 70 80 90 100
FLYWTGPNME VDTLDISSIR DTRTGRYARL PKDPKIREVL GFGGPDTRLE
110 120 130 140 150
EKLMTVVAGP DPVNTTFLNF MAVQDDTVKV WSEELFKLAM NILAQNASRN
160 170 180 190 200
TFLRKAYTKL KLQVNQDGRI PVKNILKMFS ADKKRVETAL ESCGLNFNRS
210 220 230 240 250
ESIRPDEFPL EIFERFLNKL CLRPDIDKIL LEIGAKGKPY LTLEQLMDFI
260 270 280 290 300
NQKQRDPRLN EVLYPPLRSS QARLLIEKYE TNKQFLERDQ MSMEGFSRYL
310 320 330 340 350
GGEENGILPL EALDLSMDMT QPLSAYFINS SHNTYLTAGQ LAGPSSVEMY
360 370 380 390 400
RQALLWGCRC VELDVWKGRP PEEEPFITHG FTMTTEVPLR DVLEAIAEAA
410 420 430 440 450
FKTSPYPVIL SFENHVDSAK QQAKMAEYCR SIFGDALLID PLDKYPLSAG
460 470 480 490 500
IPLPSPQDLM GRILVKNKKR HRPSTGVPDS SVRKRPLEQS NSALSESSAA
510 520 530 540 550
TEPSSPQLGS PSSDSCPGLS NGEEVGLEKT SLEPQKSLGE ESLSREPNVP
560 570 580 590 600
MPDRDREDEE EDEEEEETTD PKKPTTDEGT ASSEVNATEE MSTLVNYVEP
610 620 630 640 650
VKFKSFEAAR KRNKCFEMSS FVETKAMEQL TKSPMEFVEY NKQQLSRIYP
660 670 680 690 700
KGTRVDSSNY MPQLFWNVGC QLVALNFQTL DLPMQLNAGV FEYNGRSGYL
710 720 730 740 750
LKPEFMRRPD KSFDPFTEVI VDGIVANALR VKVISGQFLS DKKVGIYVEV
760 770 780 790 800
DMFGLPVDTR RKYRTRTSQG NSFNPVWDEE PFDFPKVVLP TLASLRIAAF
810 820 830 840 850
EEGGKFVGHR ILPVSAIRSG YHYVCLRNEA NQPLCLPALL IYTEASDYIP
860 870 880 890 900
DDHQDYAEAL INPIKHVSLM DQRAKQLAAL IGESEAQAST ETYQETPCQQ
910 920 930 940 950
PGSQLPSNPT PNPLDASPRW PPGPTTSSTS SSLSSPGQRD DLIASILSEV
960 970 980 990 1000
TPTPLEELRS HKAMVKLRSR QDRDLRELHK KHQRKAVALT RRLLDGLAQA
1010 1020 1030 1040 1050
RAEGKCRPSP SALGKATNSE DVKEEEEAKQ YREFQNRQVQ SLLELREAQA
1060 1070 1080 1090 1100
DVETKRKLEH LRQAHQRLKE VVLDTHTTQF KRLKELNERE KKELQKILDR
1110 1120 1130 1140 1150
KRNNSISEAK TREKHKKEVE LTEINRRHIT ESVNSIRRLE EAQKQRHERL
1160 1170 1180 1190 1200
VAGQQQVLQQ LEEEEPKLLA QLTQECQEQR ERLPQEIRRC LLGETAEGLG
1210 1220 1230
DGPLVACASN GHAPGSGGHL SSADSESQEE NTQL
Length:1,234
Mass (Da):139,487
Last modified:July 27, 2011 - v2
Checksum:iE055744A33C5F6B0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16P → S in AAA85199 (PubMed:9714794).Curated1
Sequence conflicti20E → K in AAA85199 (PubMed:9714794).Curated1
Sequence conflicti609A → S in AAA85199 (PubMed:9714794).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43144 mRNA Translation: AAA85199.1
AK146793 mRNA Translation: BAE27436.1
CH466612 Genomic DNA Translation: EDL33267.1
CCDSiCCDS70925.1
RefSeqiNP_001277278.1, NM_001290349.1
NP_032900.2, NM_008874.4
UniGeneiMm.273204

Genome annotation databases

EnsembliENSMUST00000025912; ENSMUSP00000025912; ENSMUSG00000024960
GeneIDi18797
KEGGimmu:18797
UCSCiuc008gjr.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPLCB3_MOUSE
AccessioniPrimary (citable) accession number: P51432
Secondary accession number(s): Q3UIS0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: May 23, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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