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P51399

- LOX5_MESAU

UniProt

P51399 - LOX5_MESAU

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Protein
Arachidonate 5-lipoxygenase
Gene
ALOX5
Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes.

Catalytic activityi

Arachidonate + O2 = leukotriene A4 + H2O.

Cofactori

Binds 1 iron ion per subunit By similarity.
Binds 2 calcium ions per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi17 – 171Calcium 1; via carbonyl oxygen; structural By similarity
Metal bindingi18 – 181Calcium 2; via carbonyl oxygen; structural By similarity
Metal bindingi19 – 191Calcium 2; structural By similarity
Metal bindingi44 – 441Calcium 2; structural By similarity
Metal bindingi45 – 451Calcium 2; via carbonyl oxygen; structural By similarity
Metal bindingi47 – 471Calcium 2; structural By similarity
Metal bindingi79 – 791Calcium 1; via carbonyl oxygen; structural By similarity
Metal bindingi80 – 801Calcium 1; via carbonyl oxygen; structural By similarity
Sitei103 – 1031Essential for stabilizing binding to COTL1 By similarity
Metal bindingi367 – 3671Iron; catalytic By similarity
Metal bindingi372 – 3721Iron; catalytic By similarity
Metal bindingi550 – 5501Iron; catalytic By similarity
Metal bindingi554 – 5541Iron; catalytic By similarity
Metal bindingi673 – 6731Iron; via carboxylate; catalytic By similarity

GO - Molecular functioni

  1. arachidonate 5-lipoxygenase activity Source: UniProtKB
  2. iron ion binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. leukotriene biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Biological processi

Leukotriene biosynthesis

Keywords - Ligandi

Calcium, Iron, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00877.

Names & Taxonomyi

Protein namesi
Recommended name:
Arachidonate 5-lipoxygenase (EC:1.13.11.34)
Short name:
5-LO
Short name:
5-lipoxygenase
Gene namesi
Name:ALOX5
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Cytoplasm By similarity. Nucleus matrix By similarity. Nucleus membrane; Peripheral membrane protein By similarity
Note: Shuttles between cytoplasm and nucleus. Found exclusively in the nucleus, when phosphorylated on Ser-271. Calcium binding promotes translocation from the cytosol and the nuclear matrix to the nuclear envelope and membrane association By similarity.

GO - Cellular componenti

  1. cytosol Source: UniProtKB
  2. nuclear envelope lumen Source: UniProtKB
  3. nuclear matrix Source: UniProtKB-SubCell
  4. nuclear membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 673673Arachidonate 5-lipoxygenase
PRO_0000220694Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei271 – 2711Phosphoserine By similarity
Modified residuei523 – 5231Phosphoserine By similarity

Post-translational modificationi

Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-271 prevents export from the nucleus By similarity.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with ALOX5AP and LTC4S. Interacts with COTL1, the interaction is required for stability and efficient catalytic activity By similarity.

Structurei

3D structure databases

ProteinModelPortaliP51399.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 117116PLAT
Add
BLAST
Domaini118 – 673556Lipoxygenase
Add
BLAST

Sequence similaritiesi

Belongs to the lipoxygenase family.
Contains 1 PLAT domain.

Phylogenomic databases

HOVERGENiHBG005150.

Family and domain databases

Gene3Di2.60.60.20. 1 hit.
InterProiIPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view]
PANTHERiPTHR11771. PTHR11771. 1 hit.
PfamiPF00305. Lipoxygenase. 2 hits.
PF01477. PLAT. 1 hit.
[Graphical view]
PRINTSiPR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTiSM00308. LH2. 1 hit.
[Graphical view]
SUPFAMiSSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEiPS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51399-1 [UniParc]FASTAAdd to Basket

« Hide

MPSYTVTVAT GSQWFAGTDD YIYLSLIGSA GCSEKHLLDK AFYNDFERGA    50
VDSYDVTVDE ELGEIQLVRI EKRKYWLHDD WYLKYITLKT PTDYIEFPCY 100
RWITGEGEIV LRDGRAKLAR DDQIHILKQH RRKELEARQK QYRWMEWNPG 150
FPLSIDAKCH KDLPRDIQFD SEKGVDFVLN YSKAMENLFI NRFMHMFQSS 200
WNDFADFEKI FVKISNTISE RVKNHWQEDL MFGYQFLNGC NPVLIKRCRE 250
LPQKLPVTTE MVECSLERHL SLEQEVQEGN IFIVDYELLD GIDANKTDPC 300
THQFLAAPIC LLYKNLANKI VPIAIQLNQA PGEKNPIFLP SDAKYDWLLA 350
KIWVRSSDFH VHQTITHLLC THLVSEVFGI AMYRQLPAVH PIFKLLVAHV 400
RFTIAINTKA REQLICEYGL FDKANATGGG GHVQMVQRAV QDLTYSSLCF 450
PEAIKARGMD STEDIPYYFY RDDGLLVWEA IQSFTSEVVS IYYEDDQVVM 500
EDQELQDFVK DVYVYGMRGR KASGFPKSIK SREKLSEYLT VVIFTASAQH 550
AAVNFGQYDW CSWIPNAPPT MRAPPATAKG VVTIEQIVAT LPDRGRSCWH 600
LGAVWALSQF QENELFLGMY PEEHFIEKPV KEAMTRFRKN LEAIVNVIAE 650
RNKNKKLPYY YLSPDRIPNS VAI 673
Length:673
Mass (Da):77,873
Last modified:January 23, 2007 - v2
Checksum:iA7AF63B11CDC7972
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43333 mRNA. Translation: AAA85257.1.
RefSeqiNP_001268516.1. NM_001281587.1.

Genome annotation databases

GeneIDi101839970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U43333 mRNA. Translation: AAA85257.1 .
RefSeqi NP_001268516.1. NM_001281587.1.

3D structure databases

ProteinModelPortali P51399.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101839970.

Organism-specific databases

CTDi 240.

Phylogenomic databases

HOVERGENi HBG005150.

Enzyme and pathway databases

UniPathwayi UPA00877 .

Family and domain databases

Gene3Di 2.60.60.20. 1 hit.
InterProi IPR008976. Lipase_LipOase.
IPR000907. LipOase.
IPR013819. LipOase_C.
IPR020834. LipOase_CS.
IPR020833. LipOase_Fe_BS.
IPR001885. LipOase_mml.
IPR001024. PLAT/LH2_dom.
[Graphical view ]
PANTHERi PTHR11771. PTHR11771. 1 hit.
Pfami PF00305. Lipoxygenase. 2 hits.
PF01477. PLAT. 1 hit.
[Graphical view ]
PRINTSi PR00087. LIPOXYGENASE.
PR00467. MAMLPOXGNASE.
SMARTi SM00308. LH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48484. SSF48484. 1 hit.
SSF49723. SSF49723. 1 hit.
PROSITEi PS00711. LIPOXYGENASE_1. 1 hit.
PS00081. LIPOXYGENASE_2. 1 hit.
PS51393. LIPOXYGENASE_3. 1 hit.
PS50095. PLAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning, sequencing and expression of a 5-lipoxygenase from Syrian hamster embryo fibroblasts."
    Kitzler J.W., Eling T.E.
    Prostaglandins Leukot. Essent. Fatty Acids 55:269-277(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Syrian.

Entry informationi

Entry nameiLOX5_MESAU
AccessioniPrimary (citable) accession number: P51399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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