P51375 (GLTB_PORPU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferredoxin-dependent glutamate synthase
EC=1.4.7.1

Alternative name(s):
Fd-GOGAT

Gene names

Name:

gltB

Encoded on

Plastid; Chloroplast

Organism

Porphyra purpurea

Taxonomic identifier

2787 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Bangiales › Bangiaceae › Porphyra

Protein attributes

Sequence length	1538 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.
Cofactor	Binds 1 3Fe-4S cluster By similarity. FAD By similarity. FMN By similarity.
Pathway	Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1. Energy metabolism; nitrogen metabolism.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast stroma.
Sequence similarities	Belongs to the glutamate synthase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Glutamate biosynthesis
Cellular component	Chloroplast Plastid
Domain	Glutamine amidotransferase
Ligand	3Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate synthase (ferredoxin) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1538	1538	Ferredoxin-dependent glutamate synthase		PRO_0000170792
Regions
	Domain	34 – 431	398	Glutamine amidotransferase type-2
	Nucleotide binding	1109 – 1166	58	FMN By similarity
Sites
	Active site	34	1	For GATase activity By similarity
	Metal binding	1162	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1168	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1173	1	Iron-sulfur (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51375 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 3F647CDB2F5C77CF	FASTA	1,538	168,711
	10 20 30 40 50 60 MFNQKIIEQA SGKLTGSLTK SSSLVSIEKE RDACGVGFIA DVNNIANHKI VVQALEALTC 70 80 90 100 110 120 MEHRGACSAD RDSGDGAGIT TAIPWNLFQS GLKDKGIIIQ KNESIGVGML FLPTSKLQES 130 140 150 160 170 180 KKIIENVLKE ENLEVVGWRL VPTVEEVLGK QAYLNKPHVE QMFCRSSNLS KNELEQQLFL 190 200 210 220 230 240 VRKKIERYIG INGKEWAHEF YVCSLSCYTI VYKGMMRSAV LGQFYQDLYH SEYTSSFAIY 250 260 270 280 290 300 HRRFSTNTMP KWPLAQPMRF ISHNGEINTL LGNLNWMRSR EPLLKSPIWK NRIDELKPIT 310 320 330 340 350 360 NKDNSDSANL DAAVELLIAS GRSAEEALMI LVPEAFQNQP EFNKNTEISD FYEYYSGLQE 370 380 390 400 410 420 PWDGPALVVF TDGKVIGATL DRNGLRPARY VITKDNLVIV SSESGVVQVE PSNIKSKGRL 430 440 450 460 470 480 GPGQMISVDI ISHKILNNKE IKTSVAGKTP YGDLLKESRQ ILGHQAFFSE QQVESKKLMQ 490 500 510 520 530 540 LQTAFGYTNE DVELVIEHMA SQGKEPTFCM GDDIPLAILS EKSHILYDYF KQRFAQVTNP 550 560 570 580 590 600 AIDPLRESLV MSLTIQIGHK SNLLDDQPVL AKHIKLDSPI INEGELNAIL ESKLSCAHIN 610 620 630 640 650 660 TIFKVEKGPN DFKKQIEQLC ESASQAILSG NNILILSDKN DILESEKVSI PPLLAAGAVH 670 680 690 700 710 720 HHLINKGLRQ DASIIIETAQ CWSTHHFACL IGYGASAICP YLAFETARHW WSNPKTKMLM 730 740 750 760 770 780 SKGRLPACNI QEAQANYKKA VEAGLLKILS KMGISLLSSY HGAQIFEILG LGSEVVNFAF 790 800 810 820 830 840 KGTTSQIGGL SMEELGQETV NIHSKAFSQV KTKKLANYGF VQYRPGGEYH INNPEMSKAL 850 860 870 880 890 900 HQAVRGYNPE YYNSYQRLLQ NRPPTALRDL LKLKSNKQPI AIDKVESMEN ILHKFCTGGM 910 920 930 940 950 960 SLGALSRETH ETLAIAMNRI GGKSNSGEGG EDPVRFKVLN DVNESGNSDL LPHLKGLRNG 970 980 990 1000 1010 1020 DTASSAIKQI ASGRFGVTPE YLMNAKQLEI KIAQGAKPGE GGQLPGKKIS PYIATLRKCK 1030 1040 1050 1060 1070 1080 PGVPLISPPP HHDIYSIEDL SQLIFDLHQI NPTAKISVKL VSEIGIGTIA AGVAKGNADI 1090 1100 1110 1120 1130 1140 IQISGHDGGT GASPLSSIKH AGSPWELGLS EVHQLLAENQ LRDRVTLRVD GGLRTGSDIV 1150 1160 1170 1180 1190 1200 LAAIMGAEEF GFGTIAMIAT GCIMARICHT NKCPVGVATQ REELRARFSG VPEALVNFFL 1210 1220 1230 1240 1250 1260 FIGNEVREIL ASLGYKSLDE ITGQNHLLIK NTDIELAKTR GIELNSLINI NTHTWTKFNS 1270 1280 1290 1300 1310 1320 VHTNGPVMDD DILAIPEIND AIKLENEVAK HFKIANTNRT VGTRLSGVIA QKYGNEGFKG 1330 1340 1350 1360 1370 1380 LIKLNFYGSA GQSFGAFLAS GVNLKLMGEA NDYVGKGMNG GSIIIVPPAG TTYEDNNQVI 1390 1400 1410 1420 1430 1440 IGNTCLYGAT GGYLFAQGQA GERFAVRNSL AKSVVEGVGD HACEYMTGGT IVVLGKAGRN 1450 1460 1470 1480 1490 1500 VGAGMTGGLA YFLDEENKFI ERVNSEIVKV QRVITKAGEQ QLKNLIENHS AKTGSLKAHN 1510 1520 1530 ILENWNTYLP QFWQVVPPSE ANIEETNTAY SSNTITAY

References

[1]	"Complete nucleotide sequence of the Porphyra purpurea chloroplast genome." Reith M.E., Munholland J. Plant Mol. Biol. Rep. 13:333-335(1995) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Avonport.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U38804 Genomic DNA. Translation: AAC08261.1.
PIR	S73296.
RefSeq	NP_053985.1. NC_000925.1.
3D structure databases
ProteinModelPortal	P51375.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	810015.
Phylogenomic databases
ProtClustDB	CLSC624839.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000583. GATase_2. IPR017932. GATase_II. IPR002489. Glu_synth_asu_C. IPR002932. Glu_synth_centr_C. IPR006982. Glu_synth_centr_N. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 2 hits. G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF04898. Glu_syn_central. 1 hit. PF01645. Glu_synthase. 1 hit. PF01493. GXGXG. 1 hit. [Graphical view]
SUPFAM	SSF69336. Glu_synthase_C. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTB_PORPU

Accession

Primary (citable) accession number: P51375

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	September 21, 2011
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)