Reviewed, UniProtKB/Swiss-Prot P51277 (ACSF_PORPU)

Names and origin

Protein names

Recommended name:
    Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase
      Short name=Mg-protoporphyrin IX monomethyl ester oxidative cyclase
    EC=1.14.13.81

Gene names

Name:	acsF
Synonyms:	ycf59

Encoded on

Plastid; Chloroplast

Organism

Porphyra purpurea

Taxonomic identifier

2787 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Bangiales › Bangiaceae › Porphyra

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) By similarity.
Catalytic activity	Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + H2O. HAMAP MF_01840 131-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP+ + 2 H2O. HAMAP MF_01840 131-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O2 = divinylprotochlorophyllide + NADP+ + 2 H2O. HAMAP MF_01840
Cofactor	Iron By similarity.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis (light-independent). HAMAP MF_01840
Subcellular location	Plastid › chloroplast. HAMAP MF_01840
Sequence similarities	Belongs to the acsF family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Ligand	Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	iron ion binding Inferred from electronic annotation. Source: HAMAP magnesium-protoporphyrin IX monomethyl ester (oxidative) cyclase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 349	349	Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase HAMAP MF_01840		PRO_0000217544

Sequences

	Sequence		Length	Mass (Da)
	P51277-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2F46724F9D513D43	FASTA	349	41,550
	10 20 30 40 50 60 MQTTIQKEEA PNRETLLTPR FYTTDFEEMA SMNISENEQD FFAILEEFRA DYNSQHFIRG 70 80 90 100 110 120 EEFNQSWSNL ETKTKSLFIE FLERSCTAEF SGFLLYKELS RKLKDSNPII AECFLLMSRD 130 140 150 160 170 180 EARHAGFLNK AIGDFNLSLD LGFLTKTRKY TFFSPKFIFY ATYLSEKIGY WRYITIYRHM 190 200 210 220 230 240 EQYPEHRIYP IFKFFENWCQ DENRHGDFFA ALLKSQPHFL NDWKAKMWCR FFLLSVFATM 250 260 270 280 290 300 YLNDFQRIDF YNAIGLDSRQ YDMQVIRKTN ESAARVFPVA LDVDNPKFFK YLDSCACNNR 310 320 330 340 ALIDIDKKGS QPLVKTFMKA PLYMSLILNL IKIYLIKPID SQAVWNTVR

References

[1]	"Complete nucleotide sequence of the Porphyra purpurea chloroplast genome." Reith M.E., Munholland J. Plant Mol. Biol. Rep. 13:333-335(1995) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Avonport.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U38804 Genomic DNA. Translation: AAC08163.1.
PIR	S73198.
RefSeq	NP_053887.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	809907.
Enzyme and pathway databases
BRENDA	1.14.13.81. 279956.
Family and domain databases
HAMAP	MF_01840. [Tree]
InterPro	IPR008434. AcsF. IPR003251. Rubrerythrin. [Graphical view]
Pfam	PF02915. Rubrerythrin. 1 hit. [Graphical view]
TIGRFAMs	TIGR02029. AcsF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACSF_PORPU

Accession

Primary (citable) accession number: P51277

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)