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Protein

Calpain-3

Gene

CAPN3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.

Catalytic activityi

Broad endopeptidase activity.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei129PROSITE-ProRule annotation1
Active sitei335PROSITE-ProRule annotation1
Active sitei359PROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi663 – 6731By similarityAdd BLAST11
Calcium bindingi706 – 7172PROSITE-ProRule annotationBy similarityAdd BLAST12
Calcium bindingi736 – 7423PROSITE-ProRule annotationBy similarity7
Calcium bindingi801 – 8074By similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
LigandCalcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.54 908

Protein family/group databases

MEROPSiC02.004

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-3 (EC:3.4.22.54)
Alternative name(s):
Calcium-activated neutral proteinase 3
Short name:
CANP 3
Calpain L3
Calpain p94
Muscle-specific calcium-activated neutral protease 3
New calpain 1
Short name:
nCL-1
Gene namesi
Name:CAPN3
Synonyms:NCL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077051 – 822Calpain-3Add BLAST822

Proteomic databases

PaxDbiP51186
PRIDEiP51186

Expressioni

Tissue specificityi

Skeletal muscle.

Interactioni

Subunit structurei

Homodimer; via EF-hand domain 4. Interacts with TTN/titin. Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011677

Structurei

3D structure databases

ProteinModelPortaliP51186
SMRiP51186
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 418Calpain catalyticPROSITE-ProRule annotationAdd BLAST345
Domaini650 – 684EF-hand 1PROSITE-ProRule annotationAdd BLAST35
Domaini693 – 726EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini723 – 758EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini788 – 822EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni419 – 587Domain IIIAdd BLAST169
Regioni588 – 650LinkerAdd BLAST63
Regioni651 – 822Domain IVAdd BLAST172

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045 Eukaryota
ENOG410XP0B LUCA
HOGENOMiHOG000232035
HOVERGENiHBG012645
InParanoidiP51186
KOiK08573

Family and domain databases

CDDicd00214 Calpain_III, 1 hit
cd00044 CysPc, 1 hit
InterProiView protein in InterPro
IPR033883 C2_III
IPR022684 Calpain_cysteine_protease
IPR022682 Calpain_domain_III
IPR022683 Calpain_III
IPR036213 Calpain_III_sf
IPR029531 CAPN3
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR002048 EF_hand_dom
IPR038765 Papain_like_cys_pep_sf
IPR000169 Pept_cys_AS
IPR001300 Peptidase_C2_calpain_cat
PANTHERiPTHR10183:SF329 PTHR10183:SF329, 1 hit
PfamiView protein in Pfam
PF01067 Calpain_III, 1 hit
PF13202 EF-hand_5, 1 hit
PF13833 EF-hand_8, 1 hit
PF00648 Peptidase_C2, 1 hit
PRINTSiPR00704 CALPAIN
SMARTiView protein in SMART
SM00720 calpain_III, 1 hit
SM00230 CysPc, 1 hit
SM00054 EFh, 3 hits
SUPFAMiSSF47473 SSF47473, 1 hit
SSF49758 SSF49758, 1 hit
SSF54001 SSF54001, 2 hits
PROSITEiView protein in PROSITE
PS50203 CALPAIN_CAT, 1 hit
PS00018 EF_HAND_1, 2 hits
PS50222 EF_HAND_2, 4 hits
PS00139 THIOL_PROTEASE_CYS, 1 hit

Sequencei

Sequence statusi: Complete.

P51186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTVISASVA PRTGAEPMSP GPIAQAAQDK GTEAGGGNPS GIYSAIISRN
60 70 80 90 100
FPIIGVKEKT FEQLHKKCLE KKVLFVDPEF PPDETSLFYS QKFPIQFVWK
110 120 130 140 150
RPPEICENPR FIVGGANRTD ICQGDLGDCW FLAAIACLTL NKRLLFRVIP
160 170 180 190 200
HDQSFTENYA GIFHFQFWRY GDWVDVVIDD CLPTYNNQLV FTKSNHRNEF
210 220 230 240 250
WNALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVTEFFE IKDAPRDMYK
260 270 280 290 300
IMKKAIERGS LMGCSIDDGT NMTYGTSPSG LKMGELIERM VRNMDNSRLR
310 320 330 340 350
DSDLIPEGCS DDRPTRMIVP VQFETRMACG LVKGHAYSVT GLEEALYKGE
360 370 380 390 400
KVKLVRLRNP WGQVEWNGSW SDSWKDWSYV DKDEKARLQH QVTEDGEFWM
410 420 430 440 450
SYDDFIYHFT KLEICNLTAD ALESDKLQTW TVSVNEGRWV RGCSAGGCRN
460 470 480 490 500
FPDTFWTNPQ YRLKLLEEDD DPDDSEVICS FLVALMQKNR RKDRKLGANL
510 520 530 540 550
FTIGFAIYEV PKEMHGNKQH LQKDFFLYNA SKARSRTYIN MREVSERFRL
560 570 580 590 600
PPSEYVIVPS TYEPHQEGEF ILRVFSEKRN LSEEVENTIS VDRPVKKKKN
610 620 630 640 650
KPIIFVSDRA NSNKELGVDQ ETEEGKDNTS PDKQAKSPQL EPGNTDQESE
660 670 680 690 700
EQRQFRNIFR QIAGDDMEIC ADELKNVLNR VVNKHKDLKT QGFTLESCRS
710 720 730 740 750
MIALMDTDGS GRLNLQEFHH LWKKIKTWQK IFKHYDTDQS GTINSYEMRN
760 770 780 790 800
AVKDAGFHLN NQLYDIITMR YADKYMNIDF DSFICCFVRL EGMFRAFNAF
810 820
DKDGDGIIKL NVLEWLQLTM YA
Length:822
Mass (Da):94,603
Last modified:June 16, 2003 - v2
Checksum:iAEDE66F67508D743
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti282K → N in AAC24459 (Ref. 2) Curated1
Sequence conflicti308 – 309GC → V in AAC24459 (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087569 mRNA Translation: AAF23261.1
U07858 mRNA Translation: AAC24459.1
AF115744 mRNA Translation: AAD05333.1
RefSeqiNP_776685.1, NM_174260.2
UniGeneiBt.3961

Genome annotation databases

GeneIDi281663
KEGGibta:281663

Similar proteinsi

Entry informationi

Entry nameiCAN3_BOVIN
AccessioniPrimary (citable) accession number: P51186
Secondary accession number(s): O97700, Q9TTH9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 16, 2003
Last modified: June 20, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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