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Protein

Calpain-3

Gene

CAPN3

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Calcium-regulated non-lysosomal thiol-protease.

Catalytic activityi

Broad endopeptidase activity.

Enzyme regulationi

Activated by micromolar concentrations of calcium and inhibited by calpastatin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei129By similarity1
Active sitei335By similarity1
Active sitei359By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi706 – 7171CuratedAdd BLAST12
Calcium bindingi736 – 7472CuratedAdd BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.4.22.54. 908.

Protein family/group databases

MEROPSiC02.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain-3 (EC:3.4.22.54)
Alternative name(s):
Calcium-activated neutral proteinase 3
Short name:
CANP 3
Calpain L3
Calpain p94
Muscle-specific calcium-activated neutral protease 3
New calpain 1
Short name:
nCL-1
Gene namesi
Name:CAPN3
Synonyms:NCL1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077051 – 822Calpain-3Add BLAST822

Proteomic databases

PaxDbiP51186.
PRIDEiP51186.

Expressioni

Tissue specificityi

Skeletal muscle.

Interactioni

Subunit structurei

Interacts with TTN/titin. Interacts with CMYA5; this interaction, which results in CMYA5 proteolysis, may protect CAPN3 from autolysis.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011677.

Structurei

3D structure databases

ProteinModelPortaliP51186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini74 – 418Calpain catalyticPROSITE-ProRule annotationAdd BLAST345
Domaini650 – 684EF-hand 1PROSITE-ProRule annotationAdd BLAST35
Domaini693 – 726EF-hand 2PROSITE-ProRule annotationAdd BLAST34
Domaini723 – 758EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini788 – 822EF-hand 4PROSITE-ProRule annotationAdd BLAST35

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni419 – 587Domain IIIAdd BLAST169
Regioni588 – 650LinkerAdd BLAST63
Regioni651 – 822Domain IVAdd BLAST172

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP51186.
KOiK08573.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029531. CAPN3.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF320. PTHR10183:SF320. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51186-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTVISASVA PRTGAEPMSP GPIAQAAQDK GTEAGGGNPS GIYSAIISRN
60 70 80 90 100
FPIIGVKEKT FEQLHKKCLE KKVLFVDPEF PPDETSLFYS QKFPIQFVWK
110 120 130 140 150
RPPEICENPR FIVGGANRTD ICQGDLGDCW FLAAIACLTL NKRLLFRVIP
160 170 180 190 200
HDQSFTENYA GIFHFQFWRY GDWVDVVIDD CLPTYNNQLV FTKSNHRNEF
210 220 230 240 250
WNALLEKAYA KLHGSYEALK GGNTTEAMED FTGGVTEFFE IKDAPRDMYK
260 270 280 290 300
IMKKAIERGS LMGCSIDDGT NMTYGTSPSG LKMGELIERM VRNMDNSRLR
310 320 330 340 350
DSDLIPEGCS DDRPTRMIVP VQFETRMACG LVKGHAYSVT GLEEALYKGE
360 370 380 390 400
KVKLVRLRNP WGQVEWNGSW SDSWKDWSYV DKDEKARLQH QVTEDGEFWM
410 420 430 440 450
SYDDFIYHFT KLEICNLTAD ALESDKLQTW TVSVNEGRWV RGCSAGGCRN
460 470 480 490 500
FPDTFWTNPQ YRLKLLEEDD DPDDSEVICS FLVALMQKNR RKDRKLGANL
510 520 530 540 550
FTIGFAIYEV PKEMHGNKQH LQKDFFLYNA SKARSRTYIN MREVSERFRL
560 570 580 590 600
PPSEYVIVPS TYEPHQEGEF ILRVFSEKRN LSEEVENTIS VDRPVKKKKN
610 620 630 640 650
KPIIFVSDRA NSNKELGVDQ ETEEGKDNTS PDKQAKSPQL EPGNTDQESE
660 670 680 690 700
EQRQFRNIFR QIAGDDMEIC ADELKNVLNR VVNKHKDLKT QGFTLESCRS
710 720 730 740 750
MIALMDTDGS GRLNLQEFHH LWKKIKTWQK IFKHYDTDQS GTINSYEMRN
760 770 780 790 800
AVKDAGFHLN NQLYDIITMR YADKYMNIDF DSFICCFVRL EGMFRAFNAF
810 820
DKDGDGIIKL NVLEWLQLTM YA
Length:822
Mass (Da):94,603
Last modified:June 16, 2003 - v2
Checksum:iAEDE66F67508D743
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti282K → N in AAC24459 (Ref. 2) Curated1
Sequence conflicti308 – 309GC → V in AAC24459 (Ref. 2) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087569 mRNA. Translation: AAF23261.1.
U07858 mRNA. Translation: AAC24459.1.
AF115744 mRNA. Translation: AAD05333.1.
RefSeqiNP_776685.1. NM_174260.2.
UniGeneiBt.3961.

Genome annotation databases

GeneIDi281663.
KEGGibta:281663.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF087569 mRNA. Translation: AAF23261.1.
U07858 mRNA. Translation: AAC24459.1.
AF115744 mRNA. Translation: AAD05333.1.
RefSeqiNP_776685.1. NM_174260.2.
UniGeneiBt.3961.

3D structure databases

ProteinModelPortaliP51186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011677.

Protein family/group databases

MEROPSiC02.004.

Proteomic databases

PaxDbiP51186.
PRIDEiP51186.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281663.
KEGGibta:281663.

Organism-specific databases

CTDi825.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
HOGENOMiHOG000232035.
HOVERGENiHBG012645.
InParanoidiP51186.
KOiK08573.

Enzyme and pathway databases

BRENDAi3.4.22.54. 908.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR029531. CAPN3.
IPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PANTHERiPTHR10183:SF320. PTHR10183:SF320. 2 hits.
PfamiPF01067. Calpain_III. 1 hit.
PF13202. EF-hand_5. 1 hit.
PF13833. EF-hand_8. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
SM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 4 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN3_BOVIN
AccessioniPrimary (citable) accession number: P51186
Secondary accession number(s): O97700, Q9TTH9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 16, 2003
Last modified: November 30, 2016
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.