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P51182

- KPYK_SPOPS

UniProt

P51182 - KPYK_SPOPS

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Protein

Pyruvate kinase

Gene

pyk

Organism
Sporosarcina psychrophila (Bacillus psychrophilus)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Magnesium.
Potassium.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321SubstrateBy similarity
Metal bindingi34 – 341PotassiumBy similarity
Metal bindingi36 – 361PotassiumBy similarity
Metal bindingi66 – 661PotassiumBy similarity
Metal bindingi67 – 671Potassium; via carbonyl oxygenBy similarity
Sitei220 – 2201Transition state stabilizerBy similarity
Metal bindingi222 – 2221MagnesiumBy similarity
Binding sitei245 – 2451Substrate; via amide nitrogenBy similarity
Metal bindingi246 – 2461MagnesiumBy similarity
Binding sitei246 – 2461Substrate; via amide nitrogenBy similarity
Binding sitei278 – 2781SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BRENDAi2.7.1.40. 685.
UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
OrganismiSporosarcina psychrophila (Bacillus psychrophilus)
Taxonomic identifieri1476 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesPlanococcaceaeSporosarcina

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586Pyruvate kinasePRO_0000112054Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP51182.
SMRiP51182. Positions 2-586.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated
In the C-terminal section; belongs to the PEP-utilizing enzyme family.Curated

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR008279. PEP-util_enz_mobile_dom.
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00391. PEP-utilizers. 1 hit.
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52009. SSF52009. 1 hit.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P51182 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRKTKIVCTI GPASESPELL EQLIEAGMNV ARLNFSHGNH AEHKARIDSI
60 70 80 90 100
RKVAREKGKV VGILLDTKGP EIRTHSMMNG KLELVTGQKI DISMTQVEGN
110 120 130 140 150
NDVFSVSYDK LIEDVNEGSV ILLDDGLIQL EVTGKDVARG LIHTLIINSG
160 170 180 190 200
SLSNNKGVNI PGVSVQLPGM TEKDAEDILF GIREGVDFIA ASFVRRASDV
210 220 230 240 250
MEIRALLENN NGSNLQIIPK IENQEGVDNI DEILNVSDGL MVARGDLGVE
260 270 280 290 300
IPPEEVPLVQ KNLIEKCNQA GKPVITATQM LDSMQRNPRP TRAEASDVAN
310 320 330 340 350
AIFDGTDAIM LSGETAAGIY PVESVQTMDR IALTTEAAID YRSVVSTRRR
360 370 380 390 400
EKHGNMTEAI GQAAAYTAIN LKVKAVLAPT ESGHTAKMIA KYRPGCPVIA
410 420 430 440 450
VTSSEMCSRK LSLIWGVYPI VGKKASSIDE ILQESVEESV KHQYVGHGDV
460 470 480 490 500
VIITAGVPVG EAGTTNLMKI HVIGDLLARG QGIGKDVAYG RTVVAKNAAE
510 520 530 540 550
ALAYDTEGAI LVTNASDRDM MPAIEKCAGL ITEEGGLTSH GAIVGLSLGI
560 570 580
PIIVGVENAT ELIQHGKEIT MDAESGVIYN GHASVL
Length:586
Mass (Da):62,571
Last modified:October 1, 1996 - v1
Checksum:i8C0B7017C9C6D5CE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D31954 Genomic DNA. Translation: BAA06725.1.
PIRiJC4219.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D31954 Genomic DNA. Translation: BAA06725.1 .
PIRi JC4219.

3D structure databases

ProteinModelPortali P51182.
SMRi P51182. Positions 2-586.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00188 .
BRENDAi 2.7.1.40. 685.

Family and domain databases

Gene3Di 2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
3.50.30.10. 1 hit.
InterProi IPR008279. PEP-util_enz_mobile_dom.
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view ]
PANTHERi PTHR11817. PTHR11817. 1 hit.
Pfami PF00391. PEP-utilizers. 1 hit.
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view ]
PRINTSi PR01050. PYRUVTKNASE.
SUPFAMi SSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52009. SSF52009. 1 hit.
SSF52935. SSF52935. 1 hit.
TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the genes for pyruvate kinase of two bacilli, Bacillus psychrophilus and Bacillus licheniformis, and comparison of the properties of the enzymes produced in Escherichia coli."
    Tanaka K., Sakai H., Ohta T., Matsuzawa H.
    Biosci. Biotechnol. Biochem. 59:1536-1542(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiKPYK_SPOPS
AccessioniPrimary (citable) accession number: P51182
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 1, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3