Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P51181

- KPYK_BACLI

UniProt

P51181 - KPYK_BACLI

Protein

Pyruvate kinase

Gene

pyk

Organism
Bacillus licheniformis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.
    Potassium.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei32 – 321SubstrateBy similarity
    Metal bindingi34 – 341PotassiumBy similarity
    Metal bindingi36 – 361PotassiumBy similarity
    Metal bindingi66 – 661PotassiumBy similarity
    Metal bindingi67 – 671Potassium; via carbonyl oxygenBy similarity
    Sitei220 – 2201Transition state stabilizerBy similarity
    Metal bindingi222 – 2221MagnesiumBy similarity
    Binding sitei245 – 2451Substrate; via amide nitrogenBy similarity
    Metal bindingi246 – 2461MagnesiumBy similarity
    Binding sitei246 – 2461Substrate; via amide nitrogenBy similarity
    Binding sitei278 – 2781SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    BRENDAi2.7.1.40. 669.
    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase (EC:2.7.1.40)
    Short name:
    PK
    Gene namesi
    Name:pyk
    OrganismiBacillus licheniformis
    Taxonomic identifieri1402 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 585585Pyruvate kinasePRO_0000112053Add
    BLAST

    Proteomic databases

    PRIDEiP51181.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP51181.
    SMRiP51181. Positions 2-585.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated
    In the C-terminal section; belongs to the PEP-utilizing enzyme family.Curated

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    3.50.30.10. 1 hit.
    InterProiIPR008279. PEP-util_enz_mobile_dom.
    IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00391. PEP-utilizers. 1 hit.
    PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52009. SSF52009. 1 hit.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51181-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKTKIVCTI GPASESVEKL TQLMEAGMNV ARLNFSHGDF EEHGARIKNI    50
    REAAGKLGKD IGILLDTKGP EIRTHTMENG SIELAAGSQL IVSMDEVIGT 100
    PDKISVTYDG LIHDVSVGST ILLDDGLVGL EVTDINKDKR EIVTKVMNSG 150
    TLKNKKGVNV PGVSVNLPGI TEKDANDIVF GIEQGVDFIA ASFVRRPSDV 200
    LEIRELLEEH NAADIQIIPK IENQEGVDNI DAILEVSDGL MVARGDLGVE 250
    IPAEEVPLVQ KELIKKCNAL GKPVITATQM LDSMQRNPRP TRAEASDVAN 300
    AIFDGTDAIM LSGETAAGNY PVEAVQTMHN IASRSEEALN HKKILSARSK 350
    QVSMSITDAI GQSVAHTAIN LDVNAIVTPT ESGHTARMIS KYRPQAPIVA 400
    VTVNDAVSRK LSLVFGVFAT SGQNHSSTDE MLEKAVQKSL DTGIVRHGDL 450
    IVITAGAVGE AGTTNLMKVY VVGDVVAKGQ GIGRKSAFGE VVIAQNAQEA 500
    AKKMKDGAVL VTKSTDRDMM ASLEKAAALI TEEGGLTSHA AVVGLSLGIP 550
    VIVGMENATS ILKEGEDITV DSARGAVYKG RASVL 585
    Length:585
    Mass (Da):61,943
    Last modified:October 1, 1996 - v1
    Checksum:iCC2A5391827D5660
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31955 Genomic DNA. Translation: BAA06727.1.
    PIRiJC4220.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31955 Genomic DNA. Translation: BAA06727.1 .
    PIRi JC4220.

    3D structure databases

    ProteinModelPortali P51181.
    SMRi P51181. Positions 2-585.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P51181.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .
    BRENDAi 2.7.1.40. 669.

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    3.50.30.10. 1 hit.
    InterProi IPR008279. PEP-util_enz_mobile_dom.
    IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00391. PEP-utilizers. 1 hit.
    PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52009. SSF52009. 1 hit.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the genes for pyruvate kinase of two bacilli, Bacillus psychrophilus and Bacillus licheniformis, and comparison of the properties of the enzymes produced in Escherichia coli."
      Tanaka K., Sakai H., Ohta T., Matsuzawa H.
      Biosci. Biotechnol. Biochem. 59:1536-1542(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiKPYK_BACLI
    AccessioniPrimary (citable) accession number: P51181
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3