Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Guanylyl cyclase-activating protein 2

Gene

GUCA1B

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Stimulates guanylyl cyclase 1 (GC1) and GC2 when free calcium ions concentration is low, and GC1 and GC2 when free calcium ions concentration is elevated. This Ca2+-sensitive regulation of GC is a key event in recovery of the dark state of rod photoreceptors following light exposure.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi69 – 801Add BLAST12
Calcium bindingi105 – 1162Add BLAST12
Calcium bindingi158 – 1693Add BLAST12

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Guanylyl cyclase-activating protein 2
Short name:
GCAP 2
Alternative name(s):
Guanylate cyclase activator 1B
Retinal guanylyl cyclase activator protein p24
Gene namesi
Name:GUCA1B
Synonyms:GCAP-2, GCAP2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi104Y → C: Partial loss of activity but no loss of calcium sensitivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00000738072 – 204Guanylyl cyclase-activating protein 2Add BLAST203

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineCurated1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP51177.
PRIDEiP51177.

PTM databases

iPTMnetiP51177.

Expressioni

Tissue specificityi

Retina. Appears to be more abundant in rods.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035936.

Structurei

Secondary structure

1204
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 12Combined sources5
Beta strandi13 – 15Combined sources3
Helixi18 – 32Combined sources15
Beta strandi33 – 35Combined sources3
Helixi42 – 50Combined sources9
Beta strandi53 – 55Combined sources3
Helixi58 – 68Combined sources11
Beta strandi71 – 76Combined sources6
Helixi78 – 88Combined sources11
Helixi95 – 104Combined sources10
Helixi114 – 130Combined sources17
Helixi146 – 157Combined sources12
Helixi167 – 174Combined sources8
Turni175 – 179Combined sources5
Helixi180 – 185Combined sources6
Beta strandi186 – 188Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JBANMR-A1-204[»]
ProteinModelPortaliP51177.
SMRiP51177.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP51177.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 54EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini56 – 91EF-hand 2PROSITE-ProRule annotationAdd BLAST36
Domaini92 – 127EF-hand 3PROSITE-ProRule annotationAdd BLAST36
Domaini145 – 180EF-hand 4PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP51177.
KOiK08328.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR015756. GCAP-2.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF11. PTHR23055:SF11. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQQFSWEEA EENGAVGAAD AAQLQEWYKK FLEECPSGTL FMHEFKRFFK
60 70 80 90 100
VPDNEEATQY VEAMFRAFDT NGDNTIDFLE YVAALNLVLR GTLEHKLKWT
110 120 130 140 150
FKIYDKDRNG CIDRQELLDI VESIYKLKKA CSVEVEAEQQ GKLLTPEEVV
160 170 180 190 200
DRIFLLVDEN GDGQLSLNEF VEGARRDKWV MKMLQMDLNP SSWISQQRRK

SAMF
Length:204
Mass (Da):23,728
Last modified:January 23, 2007 - v2
Checksum:iBA375D54960E5F75
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32856 mRNA. Translation: AAC48478.1.
L43001 mRNA. Translation: AAA83214.1.
PIRiA57604.
RefSeqiNP_777211.1. NM_174786.1.
UniGeneiBt.520.

Genome annotation databases

GeneIDi286851.
KEGGibta:286851.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U32856 mRNA. Translation: AAC48478.1.
L43001 mRNA. Translation: AAA83214.1.
PIRiA57604.
RefSeqiNP_777211.1. NM_174786.1.
UniGeneiBt.520.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JBANMR-A1-204[»]
ProteinModelPortaliP51177.
SMRiP51177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000035936.

PTM databases

iPTMnetiP51177.

Proteomic databases

PaxDbiP51177.
PRIDEiP51177.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi286851.
KEGGibta:286851.

Organism-specific databases

CTDi2979.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
HOGENOMiHOG000233019.
HOVERGENiHBG108179.
InParanoidiP51177.
KOiK08328.

Miscellaneous databases

EvolutionaryTraceiP51177.

Family and domain databases

CDDicd00051. EFh. 2 hits.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR015756. GCAP-2.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PTHR23055:SF11. PTHR23055:SF11. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGUC1B_BOVIN
AccessioniPrimary (citable) accession number: P51177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds three calcium ions.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.