Reviewed, UniProtKB/Swiss-Prot P51176 (TGM2_BOVIN)

Names and origin

Protein names

Recommended name:
    Protein-glutamine gamma-glutamyltransferase 2
    EC=2.3.2.13
Alternative name(s):
    Tissue transglutaminase
    TGase C
      Short name=TGC
      Short name=TG(C)
    Transglutaminase-2

Gene names

Name:

TGM2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	687 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Catalytic activity	Protein glutamine + alkylamine = protein N(5)-alkylglutamine + NH3.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Tissue specificity	Highest levels are detected in the lung. Lower levels are found in the liver, spleen and heart, but not in the brain.
Induction	By retinoic acid.
Sequence similarities	Belongs to the transglutaminase superfamily. Transglutaminase family.

Ontologies

Keywords
Ligand	Calcium Metal-binding
Molecular function	Acyltransferase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	peptide cross-linking Inferred from electronic annotation. Source: InterPro
Molecular function	acyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein-glutamine gamma-glutamyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 687	686	Protein-glutamine gamma-glutamyltransferase 2		PRO_0000213705
Sites
	Active site	277	1	By similarity
	Active site	335	1	By similarity
	Active site	358	1	By similarity
	Metal binding	398	1	Calcium By similarity
	Metal binding	400	1	Calcium By similarity
	Metal binding	447	1	Calcium By similarity
	Metal binding	452	1	Calcium By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
	Modified residue	219	1	Phosphotyrosine By similarity
	Modified residue	369	1	Phosphotyrosine By similarity
	Modified residue	427	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	638	1	V → I in AAI03291. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P51176-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7BBA00F15E779944	FASTA	687	77,112
	10 20 30 40 50 60 MAEELVLERC DLELEANGRD HHTADLCRER LVVRRGQPFW LTLHFEGRNY EASVDSLTFC 70 80 90 100 110 120 AVTGPDPSEE AGTKALFRLS DATEEGAWAA VAADQRDSTL SLHLSTPANA PVGHYRLSLE 130 140 150 160 170 180 ASTGYQGSSF MLGQFTLLFN SWCPADAVYL DSDEERQEYV LTQQGFIYQG SAKFIKNIPW 190 200 210 220 230 240 NFGQFEEGIL DICLMLLDVN PKFLRNAGRD CSRRSSPVYV GRVVSGMVNC NDDQGVLLGR 250 260 270 280 290 300 WDNNYADGIS PMSWIGSVDI LRRWKRDGCQ RVKYGQCWVF AAVACTVLRC LGIPTRVVTN 310 320 330 340 350 360 YNSAHDQNSN LLIEYFRNEF GEIQSDKSEM IWNFHCWVES WMTRPDLQPG YEGWQALDPT 370 380 390 400 410 420 PQEKSEGTYC CGPVPVRAIK EGDLSTKYDA PFVFAEVNAD VVDWIRQDDG SLHKSINHSL 430 440 450 460 470 480 VVGLKISTKC VGRDDREDIT HSYKYPEGSP EEREAFTRAN HLNKLVNKEE TGVAMRIRVG 490 500 510 520 530 540 EGMNRGCDFD VFAHITNSTP EEHTGRLLLC ARTVSYNGIL GPECGTKDLL SLSLEPYSEK 550 560 570 580 590 600 SIPLRILYEK YCDCLTESNL IKVRGLLIEP AANSYLLAER DIYLENPEIK IRILGEPKQN 610 620 630 640 650 660 RKLVAEISLQ NPLTVALSGC TFTVEGAGLI EEQKTVDVPD PVEAGEEVKV RVDLLPLYVG 670 680 RHKLVVNFES DRLKAVKGFR NVIVGPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequence analysis of cDNA clones for bovine aortic-endothelial-cell transglutaminase." Nakanishi K., Nara K., Hagiwara H., Aoyama Y., Ueno H., Hirose S. Eur. J. Biochem. 202:15-21(1991) [PubMed: 1682150] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 79-95; 157-166; 242-251 AND 581-587. Tissue: Artery.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.
[3]	"Retinol-induced morphological changes of cultured bovine endothelial cells are accompanied by a marked increase in transglutaminase." Nara K., Nakanishi K., Hagiwara H., Wakita K., Kojima S., Hirose S. J. Biol. Chem. 264:19308-19312(1989) [PubMed: 2572599] [Abstract] Cited for: PROTEIN SEQUENCE OF 79-95; 157-166 AND 242-251.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60686 mRNA. Translation: CAA43097.1. BC103290 mRNA. Translation: AAI03291.1.
IPI	IPI00703915.
PIR	S19680.
RefSeq	NP_803473.1.
UniGene	Bt.5401
3D structure databases
SMR	P51176. Positions 15-687.
ModBase	Search...
Protein-protein interaction databases
STRING	P51176.
Genome annotation databases
Ensembl	ENSBTAT00000021569; ENSBTAP00000021569; ENSBTAG00000016208; Bos taurus. [Genome view]
GeneID	281528.
KEGG	bta:281528.
Organism-specific databases
CTD	281528.
Phylogenomic databases
eggNOG	maNOG06398.
HOVERGEN	P51176.
InParanoid	P51176.
Enzyme and pathway databases
BRENDA	2.3.2.13. 251.
Family and domain databases
InterPro	IPR008957. Fibronectin_typ-III-like_fold. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR002931. Transglutaminase-like. IPR008958. Transglutaminase_C. IPR013808. Transglutaminase_CS. IPR001102. Transglutaminase_N. [Graphical view]
Gene3D	G3DSA:2.60.40.30. FN_III-like. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00927. Transglut_C. 2 hits. PF01841. Transglut_core. 1 hit. PF00868. Transglut_N. 1 hit. [Graphical view]
SMART	SM00460. TGc. 1 hit. [Graphical view]
PROSITE	PS00547. TRANSGLUTAMINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TGM2_BOVIN

Accession

Primary (citable) accession number: P51176
Secondary accession number(s): Q3ZBH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)