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P51175

- PPOX_MOUSE

UniProt

P51175 - PPOX_MOUSE

Protein

Protoporphyrinogen oxidase

Gene

Ppox

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

    Catalytic activityi

    Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

    Cofactori

    Binds 1 FAD per subunit.By similarity

    Enzyme regulationi

    Inhibited by acifluorfen.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei42 – 421FAD; via amide nitrogenBy similarity
    Binding sitei257 – 2571FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei449 – 4491FAD; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi9 – 146FADBy similarity
    Nucleotide bindingi34 – 352FADBy similarity
    Nucleotide bindingi57 – 604FADBy similarity
    Nucleotide bindingi454 – 4563FADBy similarity

    GO - Molecular functioni

    1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB

    GO - Biological processi

    1. heme biosynthetic process Source: UniProtKB
    2. oxidation-reduction process Source: UniProtKB
    3. porphyrin-containing compound biosynthetic process Source: UniProtKB
    4. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
    5. response to drug Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    FAD, Flavoprotein

    Enzyme and pathway databases

    ReactomeiREACT_203298. Heme biosynthesis.
    UniPathwayiUPA00251; UER00324.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protoporphyrinogen oxidase (EC:1.3.3.4)
    Short name:
    PPO
    Gene namesi
    Name:Ppox
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:104968. Ppox.

    Subcellular locationi

    Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Intermembrane side 1 Publication

    GO - Cellular componenti

    1. integral component of mitochondrial inner membrane Source: Ensembl
    2. intrinsic component of mitochondrial inner membrane Source: UniProtKB
    3. mitochondrial membrane Source: UniProtKB
    4. mitochondrion Source: MGI

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Protoporphyrinogen oxidasePRO_0000135272Add
    BLAST

    Proteomic databases

    MaxQBiP51175.
    PaxDbiP51175.
    PRIDEiP51175.

    PTM databases

    PhosphoSiteiP51175.

    Expressioni

    Inductioni

    During erythroid differentiation.1 Publication

    Gene expression databases

    ArrayExpressiP51175.
    BgeeiP51175.
    CleanExiMM_PPOX.
    GenevestigatoriP51175.

    Interactioni

    Subunit structurei

    Monomer. Homodimer By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP51175.
    SMRiP51175. Positions 2-474.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the protoporphyrinogen oxidase family.Curated

    Phylogenomic databases

    eggNOGiCOG1232.
    GeneTreeiENSGT00390000008744.
    HOGENOMiHOG000269479.
    HOVERGENiHBG001709.
    InParanoidiP51175.
    KOiK00231.
    OMAiWIRSIRG.
    OrthoDBiEOG7DJSMB.
    PhylomeDBiP51175.
    TreeFamiTF323479.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.660.20. 1 hit.
    InterProiIPR002937. Amino_oxidase.
    IPR016040. NAD(P)-bd_dom.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P51175-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGRTVIVLGG GISGLAASYH LIRGPSPPKV ILVEGSKRLG GWIRSIRGSD    50
    GAIFELGPRG IRPAGALGAR TLLLVSELGL ESEVLPVRGD HPAAQNRFLY 100
    VGGTLHPLPS GLRGLLRPSP PFSKPLFWAG LRELLKPRGK EPDETVHSFA 150
    QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA EQTHRSILLG 200
    LLLGAGQSPQ PDSSLIRQAR AERWSQWSLR GGLEVLPQAL HNHLASKGVT 250
    VLSGQPVCGL SLQPEGRWKV SLGDSSLEAD HIISAIPASE LSKLLPAEAA 300
    PLARILSTIK AVSVAVVNLQ YRGACLPVQG FGHLVPSSED PTVLGIVYDS 350
    VAFPEQDGNP PSLRVTVMLG GYWLQKLKAA GHQLSPELFQ QQAQEAAATQ 400
    LGLKEPPSHC LVHLHKNCIP QYTIGHWQKL DSAMQFLTAQ RLPLTLAGAS 450
    YEGVAVNDCI ESGRQAAVAV LGTESNS 477
    Length:477
    Mass (Da):50,871
    Last modified:October 1, 1996 - v1
    Checksum:i8CFB48120728DE6F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti64 – 641A → T(PubMed:7607249)Curated
    Sequence conflicti66 – 661A → P(PubMed:7607249)Curated
    Sequence conflicti108 – 1081L → S in BAA08126. (PubMed:7607249)Curated
    Sequence conflicti176 – 1761R → Q in AAH02047. (PubMed:15489334)Curated
    Sequence conflicti427 – 4271W → C in BAA08126. (PubMed:7607249)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25114 mRNA. Translation: AAA96003.1.
    D45185 mRNA. Translation: BAA08126.1.
    BC002047 mRNA. Translation: AAH02047.1.
    CCDSiCCDS15487.1.
    PIRiS65684.
    S68367.
    RefSeqiNP_032937.1. NM_008911.2.
    XP_006496770.1. XM_006496707.1.
    UniGeneiMm.266888.

    Genome annotation databases

    EnsembliENSMUST00000073120; ENSMUSP00000072863; ENSMUSG00000062729.
    GeneIDi19044.
    KEGGimmu:19044.
    UCSCiuc007dns.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U25114 mRNA. Translation: AAA96003.1 .
    D45185 mRNA. Translation: BAA08126.1 .
    BC002047 mRNA. Translation: AAH02047.1 .
    CCDSi CCDS15487.1.
    PIRi S65684.
    S68367.
    RefSeqi NP_032937.1. NM_008911.2.
    XP_006496770.1. XM_006496707.1.
    UniGenei Mm.266888.

    3D structure databases

    ProteinModelPortali P51175.
    SMRi P51175. Positions 2-474.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P51175.

    Proteomic databases

    MaxQBi P51175.
    PaxDbi P51175.
    PRIDEi P51175.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000073120 ; ENSMUSP00000072863 ; ENSMUSG00000062729 .
    GeneIDi 19044.
    KEGGi mmu:19044.
    UCSCi uc007dns.1. mouse.

    Organism-specific databases

    CTDi 5498.
    MGIi MGI:104968. Ppox.

    Phylogenomic databases

    eggNOGi COG1232.
    GeneTreei ENSGT00390000008744.
    HOGENOMi HOG000269479.
    HOVERGENi HBG001709.
    InParanoidi P51175.
    KOi K00231.
    OMAi WIRSIRG.
    OrthoDBi EOG7DJSMB.
    PhylomeDBi P51175.
    TreeFami TF323479.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00324 .
    Reactomei REACT_203298. Heme biosynthesis.

    Miscellaneous databases

    ChiTaRSi PPOX. mouse.
    NextBioi 295501.
    PROi P51175.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P51175.
    Bgeei P51175.
    CleanExi MM_PPOX.
    Genevestigatori P51175.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.660.20. 1 hit.
    InterProi IPR002937. Amino_oxidase.
    IPR016040. NAD(P)-bd_dom.
    IPR027418. PPOX_C.
    IPR004572. Protoporphyrinogen_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, sequence, and expression of mouse protoporphyrinogen oxidase."
      Dailey T.A., Dailey H.A., Meissner P., Prasad A.R.
      Arch. Biochem. Biophys. 324:379-384(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
    2. "Induction of terminal enzymes for heme biosynthesis during differentiation of mouse erythroleukemia cells."
      Taketani S., Yoshinaga T., Furukawa T., Kohno H., Tokunaga R., Nishimura K., Inokuchi H.
      Eur. J. Biochem. 230:760-765(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
      Tissue: Erythroleukemia.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    4. "Organization of the terminal two enzymes of the heme biosynthetic pathway. Orientation of protoporphyrinogen oxidase and evidence for a membrane complex."
      Ferreira G.C., Andrew T.L., Karr S.W., Dailey H.A.
      J. Biol. Chem. 263:3835-3839(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Tissue: Liver.

    Entry informationi

    Entry nameiPPOX_MOUSE
    AccessioniPrimary (citable) accession number: P51175
    Secondary accession number(s): P97344, Q99M34
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3