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Protein

Protoporphyrinogen oxidase

Gene

Ppox

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Enzyme regulationi

Inhibited by acifluorfen.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421FAD; via amide nitrogenBy similarity
Binding sitei257 – 2571FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei449 – 4491FAD; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADBy similarity
Nucleotide bindingi34 – 352FADBy similarity
Nucleotide bindingi57 – 604FADBy similarity
Nucleotide bindingi454 – 4563FADBy similarity

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB

GO - Biological processi

  1. heme biosynthetic process Source: UniProtKB
  2. oxidation-reduction process Source: UniProtKB
  3. porphyrin-containing compound biosynthetic process Source: UniProtKB
  4. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  5. response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_203298. Heme biosynthesis.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:Ppox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:104968. Ppox.

Subcellular locationi

Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Intermembrane side 1 Publication

GO - Cellular componenti

  1. integral component of mitochondrial inner membrane Source: Ensembl
  2. intrinsic component of mitochondrial inner membrane Source: UniProtKB
  3. mitochondrial membrane Source: UniProtKB
  4. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Protoporphyrinogen oxidasePRO_0000135272Add
BLAST

Proteomic databases

MaxQBiP51175.
PaxDbiP51175.
PRIDEiP51175.

PTM databases

PhosphoSiteiP51175.

Expressioni

Inductioni

During erythroid differentiation.1 Publication

Gene expression databases

BgeeiP51175.
CleanExiMM_PPOX.
ExpressionAtlasiP51175. baseline and differential.
GenevestigatoriP51175.

Interactioni

Subunit structurei

Monomer. Homodimer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP51175.
SMRiP51175. Positions 2-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

eggNOGiCOG1232.
GeneTreeiENSGT00390000008744.
HOGENOMiHOG000269479.
HOVERGENiHBG001709.
InParanoidiP51175.
KOiK00231.
OMAiTIQERGK.
OrthoDBiEOG7DJSMB.
PhylomeDBiP51175.
TreeFamiTF323479.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

P51175-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRTVIVLGG GISGLAASYH LIRGPSPPKV ILVEGSKRLG GWIRSIRGSD
60 70 80 90 100
GAIFELGPRG IRPAGALGAR TLLLVSELGL ESEVLPVRGD HPAAQNRFLY
110 120 130 140 150
VGGTLHPLPS GLRGLLRPSP PFSKPLFWAG LRELLKPRGK EPDETVHSFA
160 170 180 190 200
QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA EQTHRSILLG
210 220 230 240 250
LLLGAGQSPQ PDSSLIRQAR AERWSQWSLR GGLEVLPQAL HNHLASKGVT
260 270 280 290 300
VLSGQPVCGL SLQPEGRWKV SLGDSSLEAD HIISAIPASE LSKLLPAEAA
310 320 330 340 350
PLARILSTIK AVSVAVVNLQ YRGACLPVQG FGHLVPSSED PTVLGIVYDS
360 370 380 390 400
VAFPEQDGNP PSLRVTVMLG GYWLQKLKAA GHQLSPELFQ QQAQEAAATQ
410 420 430 440 450
LGLKEPPSHC LVHLHKNCIP QYTIGHWQKL DSAMQFLTAQ RLPLTLAGAS
460 470
YEGVAVNDCI ESGRQAAVAV LGTESNS
Length:477
Mass (Da):50,871
Last modified:October 1, 1996 - v1
Checksum:i8CFB48120728DE6F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641A → T(PubMed:7607249)Curated
Sequence conflicti66 – 661A → P(PubMed:7607249)Curated
Sequence conflicti108 – 1081L → S in BAA08126. (PubMed:7607249)Curated
Sequence conflicti176 – 1761R → Q in AAH02047. (PubMed:15489334)Curated
Sequence conflicti427 – 4271W → C in BAA08126. (PubMed:7607249)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25114 mRNA. Translation: AAA96003.1.
D45185 mRNA. Translation: BAA08126.1.
BC002047 mRNA. Translation: AAH02047.1.
CCDSiCCDS15487.1.
PIRiS65684.
S68367.
RefSeqiNP_032937.1. NM_008911.2.
XP_006496770.1. XM_006496707.1.
UniGeneiMm.266888.

Genome annotation databases

EnsembliENSMUST00000073120; ENSMUSP00000072863; ENSMUSG00000062729.
GeneIDi19044.
KEGGimmu:19044.
UCSCiuc007dns.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25114 mRNA. Translation: AAA96003.1.
D45185 mRNA. Translation: BAA08126.1.
BC002047 mRNA. Translation: AAH02047.1.
CCDSiCCDS15487.1.
PIRiS65684.
S68367.
RefSeqiNP_032937.1. NM_008911.2.
XP_006496770.1. XM_006496707.1.
UniGeneiMm.266888.

3D structure databases

ProteinModelPortaliP51175.
SMRiP51175. Positions 2-474.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP51175.

Proteomic databases

MaxQBiP51175.
PaxDbiP51175.
PRIDEiP51175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073120; ENSMUSP00000072863; ENSMUSG00000062729.
GeneIDi19044.
KEGGimmu:19044.
UCSCiuc007dns.1. mouse.

Organism-specific databases

CTDi5498.
MGIiMGI:104968. Ppox.

Phylogenomic databases

eggNOGiCOG1232.
GeneTreeiENSGT00390000008744.
HOGENOMiHOG000269479.
HOVERGENiHBG001709.
InParanoidiP51175.
KOiK00231.
OMAiTIQERGK.
OrthoDBiEOG7DJSMB.
PhylomeDBiP51175.
TreeFamiTF323479.

Enzyme and pathway databases

UniPathwayiUPA00251; UER00324.
ReactomeiREACT_203298. Heme biosynthesis.

Miscellaneous databases

ChiTaRSiPpox. mouse.
NextBioi295501.
PROiP51175.
SOURCEiSearch...

Gene expression databases

BgeeiP51175.
CleanExiMM_PPOX.
ExpressionAtlasiP51175. baseline and differential.
GenevestigatoriP51175.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequence, and expression of mouse protoporphyrinogen oxidase."
    Dailey T.A., Dailey H.A., Meissner P., Prasad A.R.
    Arch. Biochem. Biophys. 324:379-384(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
  2. "Induction of terminal enzymes for heme biosynthesis during differentiation of mouse erythroleukemia cells."
    Taketani S., Yoshinaga T., Furukawa T., Kohno H., Tokunaga R., Nishimura K., Inokuchi H.
    Eur. J. Biochem. 230:760-765(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Tissue: Erythroleukemia.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  4. "Organization of the terminal two enzymes of the heme biosynthetic pathway. Orientation of protoporphyrinogen oxidase and evidence for a membrane complex."
    Ferreira G.C., Andrew T.L., Karr S.W., Dailey H.A.
    J. Biol. Chem. 263:3835-3839(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Tissue: Liver.

Entry informationi

Entry nameiPPOX_MOUSE
AccessioniPrimary (citable) accession number: P51175
Secondary accession number(s): P97344, Q99M34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.