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P51175

- PPOX_MOUSE

UniProt

P51175 - PPOX_MOUSE

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Protein

Protoporphyrinogen oxidase

Gene

Ppox

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

Binds 1 FAD per subunit.By similarity

Enzyme regulationi

Inhibited by acifluorfen.1 Publication

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei42 – 421FAD; via amide nitrogenBy similarity
Binding sitei257 – 2571FAD; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei449 – 4491FAD; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 146FADBy similarity
Nucleotide bindingi34 – 352FADBy similarity
Nucleotide bindingi57 – 604FADBy similarity
Nucleotide bindingi454 – 4563FADBy similarity

GO - Molecular functioni

  1. oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB

GO - Biological processi

  1. heme biosynthetic process Source: UniProtKB
  2. oxidation-reduction process Source: UniProtKB
  3. porphyrin-containing compound biosynthetic process Source: UniProtKB
  4. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
  5. response to drug Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

ReactomeiREACT_203298. Heme biosynthesis.
UniPathwayiUPA00251; UER00324.

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:Ppox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:104968. Ppox.

Subcellular locationi

Mitochondrion inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Intermembrane side 1 Publication

GO - Cellular componenti

  1. integral component of mitochondrial inner membrane Source: Ensembl
  2. intrinsic component of mitochondrial inner membrane Source: UniProtKB
  3. mitochondrial membrane Source: UniProtKB
  4. mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Protoporphyrinogen oxidasePRO_0000135272Add
BLAST

Proteomic databases

MaxQBiP51175.
PaxDbiP51175.
PRIDEiP51175.

PTM databases

PhosphoSiteiP51175.

Expressioni

Inductioni

During erythroid differentiation.1 Publication

Gene expression databases

BgeeiP51175.
CleanExiMM_PPOX.
ExpressionAtlasiP51175. baseline and differential.
GenevestigatoriP51175.

Interactioni

Subunit structurei

Monomer. Homodimer (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP51175.
SMRiP51175. Positions 2-474.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

eggNOGiCOG1232.
GeneTreeiENSGT00390000008744.
HOGENOMiHOG000269479.
HOVERGENiHBG001709.
InParanoidiP51175.
KOiK00231.
OMAiWIRSIRG.
OrthoDBiEOG7DJSMB.
PhylomeDBiP51175.
TreeFamiTF323479.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProiIPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view]
PfamiPF01593. Amino_oxidase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00562. proto_IX_ox. 1 hit.

Sequencei

Sequence statusi: Complete.

P51175-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGRTVIVLGG GISGLAASYH LIRGPSPPKV ILVEGSKRLG GWIRSIRGSD
60 70 80 90 100
GAIFELGPRG IRPAGALGAR TLLLVSELGL ESEVLPVRGD HPAAQNRFLY
110 120 130 140 150
VGGTLHPLPS GLRGLLRPSP PFSKPLFWAG LRELLKPRGK EPDETVHSFA
160 170 180 190 200
QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA EQTHRSILLG
210 220 230 240 250
LLLGAGQSPQ PDSSLIRQAR AERWSQWSLR GGLEVLPQAL HNHLASKGVT
260 270 280 290 300
VLSGQPVCGL SLQPEGRWKV SLGDSSLEAD HIISAIPASE LSKLLPAEAA
310 320 330 340 350
PLARILSTIK AVSVAVVNLQ YRGACLPVQG FGHLVPSSED PTVLGIVYDS
360 370 380 390 400
VAFPEQDGNP PSLRVTVMLG GYWLQKLKAA GHQLSPELFQ QQAQEAAATQ
410 420 430 440 450
LGLKEPPSHC LVHLHKNCIP QYTIGHWQKL DSAMQFLTAQ RLPLTLAGAS
460 470
YEGVAVNDCI ESGRQAAVAV LGTESNS
Length:477
Mass (Da):50,871
Last modified:October 1, 1996 - v1
Checksum:i8CFB48120728DE6F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti64 – 641A → T(PubMed:7607249)Curated
Sequence conflicti66 – 661A → P(PubMed:7607249)Curated
Sequence conflicti108 – 1081L → S in BAA08126. (PubMed:7607249)Curated
Sequence conflicti176 – 1761R → Q in AAH02047. (PubMed:15489334)Curated
Sequence conflicti427 – 4271W → C in BAA08126. (PubMed:7607249)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25114 mRNA. Translation: AAA96003.1.
D45185 mRNA. Translation: BAA08126.1.
BC002047 mRNA. Translation: AAH02047.1.
CCDSiCCDS15487.1.
PIRiS65684.
S68367.
RefSeqiNP_032937.1. NM_008911.2.
XP_006496770.1. XM_006496707.1.
UniGeneiMm.266888.

Genome annotation databases

EnsembliENSMUST00000073120; ENSMUSP00000072863; ENSMUSG00000062729.
GeneIDi19044.
KEGGimmu:19044.
UCSCiuc007dns.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U25114 mRNA. Translation: AAA96003.1 .
D45185 mRNA. Translation: BAA08126.1 .
BC002047 mRNA. Translation: AAH02047.1 .
CCDSi CCDS15487.1.
PIRi S65684.
S68367.
RefSeqi NP_032937.1. NM_008911.2.
XP_006496770.1. XM_006496707.1.
UniGenei Mm.266888.

3D structure databases

ProteinModelPortali P51175.
SMRi P51175. Positions 2-474.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P51175.

Proteomic databases

MaxQBi P51175.
PaxDbi P51175.
PRIDEi P51175.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000073120 ; ENSMUSP00000072863 ; ENSMUSG00000062729 .
GeneIDi 19044.
KEGGi mmu:19044.
UCSCi uc007dns.1. mouse.

Organism-specific databases

CTDi 5498.
MGIi MGI:104968. Ppox.

Phylogenomic databases

eggNOGi COG1232.
GeneTreei ENSGT00390000008744.
HOGENOMi HOG000269479.
HOVERGENi HBG001709.
InParanoidi P51175.
KOi K00231.
OMAi WIRSIRG.
OrthoDBi EOG7DJSMB.
PhylomeDBi P51175.
TreeFami TF323479.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00324 .
Reactomei REACT_203298. Heme biosynthesis.

Miscellaneous databases

ChiTaRSi PPOX. mouse.
NextBioi 295501.
PROi P51175.
SOURCEi Search...

Gene expression databases

Bgeei P51175.
CleanExi MM_PPOX.
ExpressionAtlasi P51175. baseline and differential.
Genevestigatori P51175.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.660.20. 1 hit.
InterProi IPR002937. Amino_oxidase.
IPR016040. NAD(P)-bd_dom.
IPR027418. PPOX_C.
IPR004572. Protoporphyrinogen_oxidase.
[Graphical view ]
Pfami PF01593. Amino_oxidase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00562. proto_IX_ox. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, sequence, and expression of mouse protoporphyrinogen oxidase."
    Dailey T.A., Dailey H.A., Meissner P., Prasad A.R.
    Arch. Biochem. Biophys. 324:379-384(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
  2. "Induction of terminal enzymes for heme biosynthesis during differentiation of mouse erythroleukemia cells."
    Taketani S., Yoshinaga T., Furukawa T., Kohno H., Tokunaga R., Nishimura K., Inokuchi H.
    Eur. J. Biochem. 230:760-765(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION.
    Tissue: Erythroleukemia.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary gland.
  4. "Organization of the terminal two enzymes of the heme biosynthetic pathway. Orientation of protoporphyrinogen oxidase and evidence for a membrane complex."
    Ferreira G.C., Andrew T.L., Karr S.W., Dailey H.A.
    J. Biol. Chem. 263:3835-3839(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Tissue: Liver.

Entry informationi

Entry nameiPPOX_MOUSE
AccessioniPrimary (citable) accession number: P51175
Secondary accession number(s): P97344, Q99M34
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3