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Protein

Protoporphyrinogen oxidase

Gene

Ppox

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX.1 Publication

Catalytic activityi

Protoporphyrinogen-IX + 3 O2 = protoporphyrin-IX + 3 H2O2.1 Publication

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Enzyme regulationi

Inhibited by acifluorfen.1 Publication

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX.
Proteins known to be involved in this subpathway in this organism are:
  1. Protoporphyrinogen oxidase (Ppox), Protoporphyrinogen oxidase (Ppox), Protoporphyrinogen oxidase (Ppox)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42FAD; via amide nitrogenBy similarity1
Binding sitei257FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei449FAD; via amide nitrogenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi9 – 14FADBy similarity6
Nucleotide bindingi34 – 35FADBy similarity2
Nucleotide bindingi57 – 60FADBy similarity4
Nucleotide bindingi454 – 456FADBy similarity3

GO - Molecular functioni

  • oxygen-dependent protoporphyrinogen oxidase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandFAD, Flavoprotein

Enzyme and pathway databases

ReactomeiR-MMU-189451 Heme biosynthesis
UniPathwayiUPA00251; UER00324

Names & Taxonomyi

Protein namesi
Recommended name:
Protoporphyrinogen oxidase (EC:1.3.3.4)
Short name:
PPO
Gene namesi
Name:Ppox
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:104968 Ppox

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001352721 – 477Protoporphyrinogen oxidaseAdd BLAST477

Proteomic databases

EPDiP51175
MaxQBiP51175
PaxDbiP51175
PeptideAtlasiP51175
PRIDEiP51175

PTM databases

iPTMnetiP51175
PhosphoSitePlusiP51175

Expressioni

Inductioni

During erythroid differentiation.1 Publication

Gene expression databases

BgeeiENSMUSG00000062729
CleanExiMM_PPOX
ExpressionAtlasiP51175 baseline and differential
GenevisibleiP51175 MM

Interactioni

Subunit structurei

Monomer. Homodimer (By similarity).By similarity

Protein-protein interaction databases

IntActiP51175, 4 interactors
STRINGi10090.ENSMUSP00000072863

Structurei

3D structure databases

ProteinModelPortaliP51175
SMRiP51175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the protoporphyrinogen oxidase family.Curated

Phylogenomic databases

eggNOGiKOG1276 Eukaryota
COG1232 LUCA
GeneTreeiENSGT00390000008744
HOGENOMiHOG000269479
HOVERGENiHBG001709
InParanoidiP51175
KOiK00231
OMAiPMICGIC
OrthoDBiEOG091G08PW
PhylomeDBiP51175
TreeFamiTF323479

Family and domain databases

Gene3Di3.50.50.60, 1 hit
InterProiView protein in InterPro
IPR002937 Amino_oxidase
IPR036188 FAD/NAD-bd_sf
IPR004572 Protoporphyrinogen_oxidase
PfamiView protein in Pfam
PF01593 Amino_oxidase, 1 hit
SUPFAMiSSF51905 SSF51905, 2 hits
TIGRFAMsiTIGR00562 proto_IX_ox, 1 hit

Sequencei

Sequence statusi: Complete.

P51175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRTVIVLGG GISGLAASYH LIRGPSPPKV ILVEGSKRLG GWIRSIRGSD
60 70 80 90 100
GAIFELGPRG IRPAGALGAR TLLLVSELGL ESEVLPVRGD HPAAQNRFLY
110 120 130 140 150
VGGTLHPLPS GLRGLLRPSP PFSKPLFWAG LRELLKPRGK EPDETVHSFA
160 170 180 190 200
QRRLGPEVAS LAMDSLCRGV FAGNSRELSI RSCFPSLFQA EQTHRSILLG
210 220 230 240 250
LLLGAGQSPQ PDSSLIRQAR AERWSQWSLR GGLEVLPQAL HNHLASKGVT
260 270 280 290 300
VLSGQPVCGL SLQPEGRWKV SLGDSSLEAD HIISAIPASE LSKLLPAEAA
310 320 330 340 350
PLARILSTIK AVSVAVVNLQ YRGACLPVQG FGHLVPSSED PTVLGIVYDS
360 370 380 390 400
VAFPEQDGNP PSLRVTVMLG GYWLQKLKAA GHQLSPELFQ QQAQEAAATQ
410 420 430 440 450
LGLKEPPSHC LVHLHKNCIP QYTIGHWQKL DSAMQFLTAQ RLPLTLAGAS
460 470
YEGVAVNDCI ESGRQAAVAV LGTESNS
Length:477
Mass (Da):50,871
Last modified:October 1, 1996 - v1
Checksum:i8CFB48120728DE6F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64A → T (PubMed:7607249).Curated1
Sequence conflicti66A → P (PubMed:7607249).Curated1
Sequence conflicti108L → S in BAA08126 (PubMed:7607249).Curated1
Sequence conflicti176R → Q in AAH02047 (PubMed:15489334).Curated1
Sequence conflicti427W → C in BAA08126 (PubMed:7607249).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U25114 mRNA Translation: AAA96003.1
D45185 mRNA Translation: BAA08126.1
BC002047 mRNA Translation: AAH02047.1
CCDSiCCDS15487.1
PIRiS65684
S68367
RefSeqiNP_032937.1, NM_008911.2
XP_006496770.1, XM_006496707.3
UniGeneiMm.266888

Genome annotation databases

EnsembliENSMUST00000073120; ENSMUSP00000072863; ENSMUSG00000062729
GeneIDi19044
KEGGimmu:19044
UCSCiuc007dns.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPPOX_MOUSE
AccessioniPrimary (citable) accession number: P51175
Secondary accession number(s): P97344, Q99M34
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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