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P51173 (APEA_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 91. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-(apurinic or apyrimidinic site) lyase

EC=4.2.99.18
Alternative name(s):
Class II apurinic/apyrimidinic(AP)-endonuclease
Gene names
Name:apeA
ORF Names:DDB_G0277701
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

Cofactor

Magnesium. Can also utilize manganese. Probably binds two magnesium or manganese ions per subunit By similarity.

Subcellular location

Nucleus Potential.

Induction

By DNA-damaging agents including UV, MNNG, gamma rays, bleomycin and streptozotocin.

Sequence similarities

Belongs to the DNA repair enzymes AP/ExoA family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361DNA-(apurinic or apyrimidinic site) lyase
PRO_0000200016

Regions

Motif41 – 444Nuclear localization signal Potential
Compositional bias27 – 3913Poly-Glu
Compositional bias70 – 8011Poly-Glu

Sites

Active site2111 By similarity
Active site2521Proton donor/acceptor By similarity
Metal binding1391Magnesium 1 By similarity
Metal binding2521Magnesium 2 By similarity
Metal binding2541Magnesium 2 By similarity
Metal binding3501Magnesium 1 By similarity
Site2541Transition state stabilizer By similarity
Site3241Important for catalytic activity By similarity
Site3511Interaction with DNA substrate By similarity

Experimental info

Sequence conflict1261Y → C in AAC47024. Ref.1
Sequence conflict2031D → V in AAC47024. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P51173 [UniParc].

Last modified December 4, 2007. Version 2.
Checksum: BBD46E1CE3B5135A

FASTA36141,327
        10         20         30         40         50         60 
MTSRTKKLKM DEEEILKKED GSETTSEEEK EEVEEEEEED KKRKLVKKTP AKKAPAKKAA 

        70         80         90        100        110        120 
AKKKSKDEDE DEEEKEEEEE TNKTTASVSI AIDNLDEPKV EENQMKIISW NVAGFKSVLS 

       130        140        150        160        170        180 
KGFTEYVEKE NPDVLCLQET KINPSNIKKD QMPKGYEYHF IEADQKGHHG TGVLTKKKPN 

       190        200        210        220        230        240 
AITFGIGIAK HDNEGRVITL EYDQFYIVNT YIPNAGTRGL QRLDYRIKEW DVDFQAYLEK 

       250        260        270        280        290        300 
LNATKPIIWC GDLNVAHTEI DLKNPKTNKK SAGFTIEERT SFSNFLEKGY VDSYRHFNPG 

       310        320        330        340        350        360 
KEGSYTFWSY LGGGRSKNVG WRLDYFVVSK RLMDSIKISP FHRTSVMGSD HCPIGVVVDL 


N 

« Hide

References

« Hide 'large scale' references
[1]"Apurinic/apyrimidinic (AP) endonuclease from Dictyostelium discoideum: cloning, nucleotide sequence and induction by sublethal levels of DNA damaging agents."
Freeland T.M., Guyer R.B., Ling A.Z., Deering R.A.
Nucleic Acids Res. 24:1950-1953(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Sequence and analysis of chromosome 2 of Dictyostelium discoideum."
Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T., Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R., Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A., Noegel A.A.
Nature 418:79-85(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
[3]"The genome of the social amoeba Dictyostelium discoideum."
Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. expand/collapse author list , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX4.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U31631 Genomic DNA. Translation: AAC47024.1.
AAFI02000021 Genomic DNA. Translation: EAL68586.1.
PIRS68268.
RefSeqXP_642518.1. XM_637426.1.

3D structure databases

ProteinModelPortalP51173.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DDB_0185018.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsDDB0185018; DDB0185018; DDB_G0277701.
GeneID8621167.
KEGGddi:DDB_G0277701.

Organism-specific databases

dictyBaseDDB_G0277701. apeA.

Phylogenomic databases

eggNOGCOG0708.
KOK10771.
OMAHETKFPA.
ProtClustDBCLSZ2453462.

Family and domain databases

Gene3D3.60.10.10. 1 hit.
InterProIPR004808. AP_endonuc_1.
IPR020847. AP_endonuclease_F1_BS.
IPR020848. AP_endonuclease_F1_CS.
IPR005135. Endo/exonuclease/phosphatase.
[Graphical view]
PANTHERPTHR22748. PTHR22748. 1 hit.
PfamPF03372. Exo_endo_phos. 1 hit.
[Graphical view]
SUPFAMSSF56219. SSF56219. 1 hit.
TIGRFAMsTIGR00633. xth. 1 hit.
PROSITEPS00726. AP_NUCLEASE_F1_1. 1 hit.
PS00727. AP_NUCLEASE_F1_2. 1 hit.
PS00728. AP_NUCLEASE_F1_3. 1 hit.
PS51435. AP_NUCLEASE_F1_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP51173.

Entry information

Entry nameAPEA_DICDI
AccessionPrimary (citable) accession number: P51173
Secondary accession number(s): Q54Z97, Q75K06
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 4, 2007
Last modified: October 16, 2013
This is version 91 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase