P51161 (FABP6_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastrotropin
Short name=GT

Alternative name(s):
Fatty acid-binding protein 6
Ileal lipid-binding protein
Short name=ILBP
Intestinal 15 kDa protein
Short name=I-15P
Intestinal bile acid-binding protein
Short name=I-BABP

Gene names

Name:	FABP6
Synonyms:	ILBP, ILLBP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	128 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ileal protein which stimulates gastric acid and pepsinogen secretion. Seems to be able to bind to bile salts and bilirubins. Isoform 2 is essential for the survival of colon cancer cells to bile acid-induced apoptosis. Ref.3
Subcellular location	Isoform 1: Cytoplasm. Isoform 2: Cytoplasm. Note: Localized close to nucleus on the apical side of both normal and neoplastic cells.
Tissue specificity	Isoform 2 is expressed in colorectal adenocarcinomas and their adjacent normal mucosa (at protein level). Isoform 1 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is expressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. Ref.3
Induction	Isoform 1 is up-regulated by chenodeoxycholic acid (CDCA) via the FXR transcription pathway. Isoform 2 is up-regulated by NF-kappa-B and in all stages of colorectal adenocarcinoma. Isoform 1 is not up-regulated in all stages of colorectal adenocarcinoma. Ref.3
Domain	Forms a beta-barrel structure that accommodates the hydrophobic ligand in its interior.
Sequence similarities	Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family.
Sequence caution	The sequence AAH22489.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cytoplasm
Coding sequence diversity	Alternative promoter usage Polymorphism
Ligand	Lipid-binding
PTM	Acetylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	bile acid and bile salt transport Traceable author statement. Source: Reactome bile acid metabolic process Traceable author statement. Source: Reactome negative regulation of cell proliferation Traceable author statement. Source: ProtInc
Cellular component	cytosol Traceable author statement. Source: Reactome
Molecular function	lipid binding Traceable author statement. Source: ProtInc transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative promoter usage. [Align] [Select]

Isoform 1 (identifier: P51161-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P51161-2)

Also known as: IBABP-L;

The sequence of this isoform differs from the canonical sequence as follows:
     1-1: M → MKTVTMMMVVEMQALTQVLRAVLSACTWVSRKGDLQRMKQTHKGKPPSSM

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 128

127

Gastrotropin

PRO_0000067380

Amino acid modifications

Modified residue

2

1

N-acetylalanine By similarity

Natural variations

Alternative sequence

1

1

M → MKTVTMMMVVEMQALTQVLR AVLSACTWVSRKGDLQRMKQ THKGKPPSSM in isoform 2.

VSP_038039

Natural variant

33

1

R → H. Ref.5
Corresponds to variant rs17856662 [ dbSNP | Ensembl ].

VAR_039578

Natural variant

55

1

S → Y. Ref.5
Corresponds to variant rs17852045 [ dbSNP | Ensembl ].

VAR_039579

Natural variant

79

1

T → M.
Corresponds to variant rs1130435 [ dbSNP | Ensembl ].

VAR_039580

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

128

Helix Strand Turn

Details...

Beta strand

4 – 14

11

Helix

15 – 22

8

Helix

26 – 33

8

Beta strand

38 – 42

5

Beta strand

47 – 54

8

Beta strand

57 – 65

9

Turn

66 – 68

3

Beta strand

70 – 72

3

Beta strand

75 – 78

4

Beta strand

80 – 82

3

Beta strand

88 – 94

7

Beta strand

99 – 104

6

Beta strand

106 – 117

12

Beta strand

119 – 126

8

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 173CBC5DBEDADDE1	FASTA	128	14,371
	10 20 30 40 50 60 MAFTGKFEME SEKNYDEFMK LLGISSDVIE KARNFKIVTE VQQDGQDFTW SQHYSGGHTM 70 80 90 100 110 120 TNKFTVGKES NIQTMGGKTF KATVQMEGGK LVVNFPNYHQ TSEIVGDKLV EVSTIGGVTY ERVSKRLA
	Isoform 2 (IBABP-L) [UniParc]. Checksum: 0ACBAE8E7DF65BF0	FASTA	177	19,874
	10 20 30 40 50 60 MKTVTMMMVV EMQALTQVLR AVLSACTWVS RKGDLQRMKQ THKGKPPSSM AFTGKFEMES 70 80 90 100 110 120 EKNYDEFMKL LGISSDVIEK ARNFKIVTEV QQDGQDFTWS QHYSGGHTMT NKFTVGKESN 130 140 150 160 170 IQTMGGKTFK ATVQMEGGKL VVNFPNYHQT SEIVGDKLVE VSTIGGVTYE RVSKRLA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and chromosomal localization of the human ileal lipid-binding protein." Oelkers P., Dawson P.A. Biochim. Biophys. Acta 1257:199-202(1995) [PubMed: 7619861] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Ileum.
[2]	"Molecular cloning, expression, and characterization of a human intestinal 15-kDa protein." Fujita M., Fujii H., Kanda T., Sato E., Hatakeyama K., Ono T. Eur. J. Biochem. 233:406-413(1995) [PubMed: 7588781] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Ileum.
[3]	"A novel variant of ileal bile acid binding protein is up-regulated through nuclear factor-kappaB activation in colorectal adenocarcinoma." Fang C., Dean J., Smith J.W. Cancer Res. 67:9039-9046(2007) [PubMed: 17909007] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, ALTERNATIVE PROMOTER USAGE, INDUCTION, TISSUE SPECIFICITY.
[4]	"The DNA sequence and comparative analysis of human chromosome 5." Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S. , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M. Nature 431:268-274(2004) [PubMed: 15372022] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), VARIANTS HIS-33 AND TYR-55. Tissue: Brain.
[6]	"The human ileal bile acid binding protein gene: promoter sequence and effects of CDX2 on expression." Barley N.F., Shaw-Smith C.J., Chakravarty P., Howard A., Legon S., Walters J.R. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-19 (ISOFORM 1).
[7]	"Insights into the bile acid transportation system: the human ileal lipid-binding protein-cholyltaurine complex and its comparison with homologous structures." Kurz M., Brachvogel V., Matter H., Stengelin S., Thuering H., Kramer W. Proteins 50:312-328(2003) [PubMed: 12486725] [Abstract] Cited for: STRUCTURE BY NMR IN COMPLEX WITH BILE ACID.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U19869 mRNA. Translation: AAB82751.1.
X90908 mRNA. Translation: CAA62415.1.
DQ132786 mRNA. Translation: ABA12611.1.
AC008609 Genomic DNA. No translation available.
AC112191 Genomic DNA. No translation available.
BC022489 mRNA. Translation: AAH22489.1. Different initiation.
AJ250902 Genomic DNA. Translation: CAB65728.1.

IPI

IPI00163119.
IPI00744927.

PIR

S63983.

RefSeq

NP_001035532.1. NM_001040442.1.
NP_001124430.1. NM_001130958.1.
NP_001436.1. NM_001445.2.

UniGene

Hs.519719.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1O1U	NMR	-	A	2-128	[»]
1O1V	NMR	-	A	2-128	[»]

ProteinModelPortal

P51161.

SMR

P51161. Positions 2-128.

ModBase

Search...

Protein-protein interaction databases

STRING

P51161.

Polymorphism databases

DMDM

1708457.

Proteomic databases

PRIDE

P51161.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000310650; ENSP00000312283; ENSG00000170231.
ENST00000393980; ENSP00000377549; ENSG00000170231.
ENST00000393982; ENSP00000377551; ENSG00000170231.
ENST00000402432; ENSP00000385433; ENSG00000170231.

GeneID

2172.

KEGG

hsa:2172.

UCSC

uc003lxx.1. human.

Organism-specific databases

CTD

2172.

GeneCards

GC05P159573.

HGNC

HGNC:3561. FABP6.

HPA

CAB024713.
HPA012601.

MIM

600422. gene.

neXtProt

NX_P51161.

PharmGKB

PA27962.

GenAtlas

Search...

Phylogenomic databases

HOVERGEN

HBG005633.

InParanoid

P51161.

OMA

TVKMEGG.

OrthoDB

EOG4S1T8K.

PhylomeDB

P51161.

Enzyme and pathway databases

Reactome

REACT_22258. Metabolism of lipids and lipoproteins.

Gene expression databases

ArrayExpress

P51161.

Bgee

P51161.

CleanEx

HS_FABP6.

Genevestigator

P51161.

GermOnline

ENSG00000170231. Homo sapiens.

Family and domain databases

InterPro

IPR012674. Calycin.
IPR011038. Calycin-like.
IPR000463. Fatty_acid-bd.
[Graphical view]

Gene3D

G3DSA:2.40.128.20. Calycin. 1 hit.

KO

K08755.

PRINTS

PR00178. FATTYACIDBP.

SUPFAM

SSF50814. Calycin. 1 hit.

PROSITE

PS00214. FABP. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

8771.

SOURCE

Search...

Entry information

Entry name

FABP6_HUMAN

Accession

Primary (citable) accession number: P51161
Secondary accession number(s): Q07DR7, Q8TBI3, Q9UGI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 109 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.