P51158 (RAB28_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-28

Gene names

Name:

Rab28

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Tissue specificity	Testis, brain, and to much lower levels heart, skeletal muscle and fat cells.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GDP binding Inferred from sequence or structural similarity. Source: UniProtKB GTP binding Inferred from sequence or structural similarity. Source: UniProtKB GTPase activity Traceable author statement Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 218	217	Ras-related protein Rab-28		PRO_0000121229
	Propeptide	219 – 221	3	Removed in mature form By similarity		PRO_0000396724
Regions
	Nucleotide binding	19 – 27	9	GTP By similarity
	Nucleotide binding	68 – 72	5	GTP By similarity
	Nucleotide binding	129 – 132	4	GTP By similarity
	Nucleotide binding	159 – 161	3	GTP By similarity
	Motif	41 – 49	9	Effector region By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine By similarity
	Modified residue	8	1	Phosphoserine By similarity
	Modified residue	218	1	Cysteine methyl ester By similarity
	Lipidation	218	1	S-farnesyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51158 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 2220A1F0B0EBFFB8	FASTA	221	24,772
	10 20 30 40 50 60 MSDSEEESQD RQLKIVVLGD GTSGKTSLAT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL 70 80 90 100 110 120 NVTLQVWDIG GQTIGGKMLD KYIYGAQGIL LVYDITNYQS FENLEDWYSV VKTVSEESET 130 140 150 160 170 180 QPLVALVGNK IDLEHMRTVK PDKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI 190 200 210 220 KLNKAEIEQS QRVVKADIVN YNQEPMSRTV NPPRSSMCAV Q

References

[1]	"Alternative mRNA splicing of the novel GTPase Rab28 generates isoforms with different C-termini." Brauers A., Schuermann A., Massmann S., Muehl-Zuerbes P., Becker W., Kainulainen H., Lie C., Joost H.-G. Eur. J. Biochem. 237:833-840(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78606 mRNA. Translation: CAA55340.1.
IPI	IPI00199252.
PIR	S65476.
RefSeq	NP_446430.1. NM_053978.1.
UniGene	Rn.4023.
3D structure databases
ProteinModelPortal	P51158.
SMR	P51158. Positions 8-184.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000023176.
PTM databases
PhosphoSite	P51158.
Proteomic databases
PaxDb	P51158.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	117049.
KEGG	rno:117049.
Organism-specific databases
CTD	9364.
RGD	620891. Rab28.
Phylogenomic databases
eggNOG	COG1100.
HOGENOM	HOG000233968.
HOVERGEN	HBG101090.
InParanoid	P51158.
KO	K07915.
OMA	CFQRIAA.
Gene expression databases
ArrayExpress	P51158.
Genevestigator	P51158.
GermOnline	ENSRNOG00000017074. Rattus norvegicus.
Family and domain databases
InterPro	IPR005225. Small_GTP-bd_dom. IPR001806. Small_GTPase. [Graphical view]
Pfam	PF00071. Ras. 1 hit. [Graphical view]
PRINTS	PR00449. RASTRNSFRMNG.
TIGRFAMs	TIGR00231. small_GTP. 1 hit.
PROSITE	PS51419. RAB. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	619871.

Entry information

Entry name

RAB28_RAT

Accession

Primary (citable) accession number: P51158

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program