Reviewed, UniProtKB/Swiss-Prot P51157 (RAB28_HUMAN)

Names and origin

Protein names

Recommended name:
    Ras-related protein Rab-28
Alternative name(s):
    Rab-26

Gene names

Name:

RAB28

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic sidePotential.
Tissue specificity	Isoform S is detected in most tissues investigated (cortex, liver, kidney, skeletal muscle, adipose tissue, testis and urothelium), whereas isoform L is predominant in testis.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Prenylation
Technical term	3D-structure
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	GTP binding Inferred from electronic annotation. Source: InterPro GTPase activity Ref.1 Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform S (identifier: P51157-1)

Also known as: Rab28S;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform L (identifier: P51157-2)

Also known as: Rab28L;

The sequence of this isoform differs from the canonical sequence as follows:
     193-221: VVKADIVNYNQEPMSRTVNPPRSSMCAVQ → IVRAEIVKYPEEENQHTTSTQSRICSVQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 221	221	Ras-related protein Rab-28		PRO_0000121227
Regions
	Nucleotide binding	19 – 26	8	GTP By similarity
	Nucleotide binding	68 – 72	5	GTP By similarity
	Nucleotide binding	129 – 132	4	GTP By similarity
	Motif	41 – 49	9	Effector region By similarity
Amino acid modifications
	Lipidation	218	1	S-farnesyl cysteine
Natural variations
	Alternative sequence	193 – 221	29	VVKAD…MCAVQ → IVRAEIVKYPEEENQHTTST QSRICSVQ in isoform L.		VSP_005530
Experimental info
	Sequence conflict	22	1	A → T in CAA64364. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform S (Rab28S) [UniParc]. Last modified January 24, 2006. Version 2. Checksum: 65AC9D6F10491916	FASTA	221	24,841
	10 20 30 40 50 60 MSDSEEESQD RQLKIVVLGD GASGKTSLTT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL 70 80 90 100 110 120 NVTLQIWDIG GQTIGGKMLD KYIYGAQGVL LVYDITNYQS FENLEDWYTV VKKVSEESET 130 140 150 160 170 180 QPLVALVGNK IDLEHMRTIK PEKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI 190 200 210 220 KLNKAEIEQS QRVVKADIVN YNQEPMSRTV NPPRSSMCAV Q
	Isoform L (Rab28L) [UniParc]. Checksum: 693F137237519E71		220	24,853
	10 20 30 40 50 60 MSDSEEESQD RQLKIVVLGD GASGKTSLTT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL 70 80 90 100 110 120 NVTLQIWDIG GQTIGGKMLD KYIYGAQGVL LVYDITNYQS FENLEDWYTV VKKVSEESET 130 140 150 160 170 180 QPLVALVGNK IDLEHMRTIK PEKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI 190 200 210 220 KLNKAEIEQS QRIVRAEIVK YPEEENQHTT STQSRICSVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alternative mRNA splicing of the novel GTPase Rab28 generates isoforms with different C-termini." Brauers A., Schuermann A., Massmann S., Muehl-Zuerbes P., Becker W., Kainulainen H., Lie C., Joost H.-G. Eur. J. Biochem. 237:833-840(1996) [PubMed: 8647132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L AND S). Tissue: Testis.
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM L). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Towards complete sets of farnesylated and geranylgeranylated proteins." Maurer-Stroh S., Koranda M., Benetka W., Schneider G., Sirota F.L., Eisenhaber F. PLoS Comput. Biol. 3:634-648(2007) [PubMed: 17411337] [Abstract] Cited for: ISOPRENYLATION AT CYS-218.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X94703 mRNA. Translation: CAA64364.1.
AF498955 mRNA. Translation: AAM21103.1.
BC035054 mRNA. Translation: AAH35054.1.

PIR

S65477.
S72399.

RefSeq

NP_001017979.1.
NP_004240.1.

UniGene

Hs.656060

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HXS	X-ray	1.10	A	8-184	[»]
2HY4	X-ray	1.50	A	8-184	[»]
3E5H	X-ray	1.50	A	8-184	[»]

ModBase

Search...

Genome annotation databases

Ensembl

ENSG00000157869. Homo sapiens. [Contig view]

GeneID

9364.

KEGG

hsa:9364.

Organism-specific databases

H-InvDB

HIX0056321.

HGNC

HGNC:9768. RAB28.

PharmGKB

PA34119.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOGENOM

P51157.

HOVERGEN

P51157.

Gene expression databases

ArrayExpress

P51157.

CleanEx

HS_RAB28.

GermOnline

ENSG00000157869. Homo sapiens.

Family and domain databases

InterPro

IPR013753. Ras.
IPR001806. Ras_trnsfrmng.
IPR005225. Small_GTP_bd.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

ProtoNet

Search...

Other Resources

LinkHub

P51157.

NextBio

35067.

Entry information

Entry name

RAB28_HUMAN

Accession

Primary (citable) accession number: P51157
Secondary accession number(s): Q8IYR8, Q8NI05

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 24, 2006
Last modified:	November 25, 2008
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)