P51157 (RAB28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-28

Gene names

Name:

RAB28

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Tissue specificity	Isoform S is detected in most tissues investigated: cortex, liver, kidney, skeletal muscle, adipose tissue, testis and urothelium. Isoform L is predominantly expressed in testis.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GDP binding Inferred from direct assay Ref.6. Source: UniProtKB GTP binding Inferred from direct assay Ref.6. Source: UniProtKB GTPase activity Traceable author statement. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform S (identifier: P51157-1)

Also known as: Rab28S;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform L (identifier: P51157-2)

Also known as: Rab28L;

The sequence of this isoform differs from the canonical sequence as follows:
     193-221: VVKADIVNYNQEPMSRTVNPPRSSMCAVQ → IVRAEIVKYPEEENQHTTSTQSRICSVQ

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 218

218

Ras-related protein Rab-28

PRO_0000121227

Propeptide

219 – 221

3

Removed in mature form Probable

PRO_0000396721

Regions

Nucleotide binding

19 – 27

9

GTP

Nucleotide binding

68 – 72

5

GTP

Nucleotide binding

129 – 132

4

GTP

Nucleotide binding

159 – 161

3

GTP

Motif

41 – 49

9

Effector region By similarity

Amino acid modifications

Modified residue

218

1

Cysteine methyl ester Probable

Lipidation

218

1

S-farnesyl cysteine Ref.4

Natural variations

Alternative sequence

193 – 221

29

VVKAD…MCAVQ → IVRAEIVKYPEEENQHTTST QSRICSVQ in isoform L.

VSP_005530

Experimental info

Sequence conflict

22

1

A → T in CAA64364. Ref.1

Secondary structure

1

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221

Helix Strand Turn

Details...

Beta strand

12 – 18

7

Helix

26 – 33

8

Helix

34 – 36

3

Helix

40 – 43

4

Beta strand

49 – 56

8

Beta strand

60 – 68

9

Helix

79 – 83

5

Beta strand

90 – 92

3

Helix

98 – 102

5

Helix

104 – 118

15

Beta strand

125 – 127

3

Helix

131 – 136

6

Helix

141 – 150

10

Helix

166 – 177

12

Sequences

	Sequence		Length	Mass (Da)
	Isoform S (Rab28S) [UniParc]. Last modified January 24, 2006. Version 2. Checksum: 65AC9D6F10491916	FASTA	221	24,841
	10 20 30 40 50 60 MSDSEEESQD RQLKIVVLGD GASGKTSLTT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL 70 80 90 100 110 120 NVTLQIWDIG GQTIGGKMLD KYIYGAQGVL LVYDITNYQS FENLEDWYTV VKKVSEESET 130 140 150 160 170 180 QPLVALVGNK IDLEHMRTIK PEKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI 190 200 210 220 KLNKAEIEQS QRVVKADIVN YNQEPMSRTV NPPRSSMCAV Q
	Isoform L (Rab28L) [UniParc]. Checksum: 693F137237519E71	FASTA	220	24,853
	10 20 30 40 50 60 MSDSEEESQD RQLKIVVLGD GASGKTSLTT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL 70 80 90 100 110 120 NVTLQIWDIG GQTIGGKMLD KYIYGAQGVL LVYDITNYQS FENLEDWYTV VKKVSEESET 130 140 150 160 170 180 QPLVALVGNK IDLEHMRTIK PEKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI 190 200 210 220 KLNKAEIEQS QRIVRAEIVK YPEEENQHTT STQSRICSVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alternative mRNA splicing of the novel GTPase Rab28 generates isoforms with different C-termini." Brauers A., Schuermann A., Massmann S., Muehl-Zuerbes P., Becker W., Kainulainen H., Lie C., Joost H.-G. Eur. J. Biochem. 237:833-840(1996) [PubMed: 8647132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L AND S). Tissue: Testis.
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM L). Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Towards complete sets of farnesylated and geranylgeranylated proteins." Maurer-Stroh S., Koranda M., Benetka W., Schneider G., Sirota F.L., Eisenhaber F. PLoS Comput. Biol. 3:634-648(2007) [PubMed: 17411337] [Abstract] Cited for: ISOPRENYLATION AT CYS-218.
[5]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[6]	"Large nucleotide-dependent conformational change in Rab28." Lee S.H., Baek K., Dominguez R. FEBS Lett. 582:4107-4111(2008) [PubMed: 19026641] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS) OF 11-184 IN COMPLEX WITH GTP AND GDP ANALOGS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X94703 mRNA. Translation: CAA64364.1.
AF498955 mRNA. Translation: AAM21103.1.
BC035054 mRNA. Translation: AAH35054.1.

IPI

IPI00016377.
IPI00219679.

PIR

S65477.
S72399.

RefSeq

NP_001017979.1. NM_001017979.2.
NP_004240.2. NM_004249.3.

UniGene

Hs.656060.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HXS	X-ray	1.10	A	11-184	[»]
3E5H	X-ray	1.50	A	11-184	[»]

ProteinModelPortal

P51157.

SMR

P51157. Positions 8-184.

ModBase

Search...

Protein-protein interaction databases

STRING

P51157.

PTM databases

PhosphoSite

P51157.

Polymorphism databases

DMDM

85700393.

Proteomic databases

PRIDE

P51157.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000330852; ENSP00000328551; ENSG00000157869.

GeneID

9364.

KEGG

hsa:9364.

UCSC

uc003gmt.1. human.
uc003gmu.1. human.

Organism-specific databases

CTD

9364.

GeneCards

GC04M013369.

H-InvDB

HIX0024565.

HGNC

HGNC:9768. RAB28.

HPA

HPA044575.

MIM

612994. gene.

neXtProt

NX_P51157.

PharmGKB

PA34119.

GenAtlas

Search...

Phylogenomic databases

eggNOG

prNOG11253.

GeneTree

ENSGT00600000084341.

HOGENOM

HBG745225.

HOVERGEN

HBG101090.

InParanoid

P51157.

OMA

IVNYSQD.

OrthoDB

EOG43R3NJ.

PhylomeDB

P51157.

Gene expression databases

ArrayExpress

P51157.

Bgee

P51157.

CleanEx

HS_RAB28.

Genevestigator

P51157.

GermOnline

ENSG00000157869. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]

KO

K07915.

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

TIGRFAMs

TIGR00231. Small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

35067.

SOURCE

Search...

Entry information

Entry name

RAB28_HUMAN

Accession

Primary (citable) accession number: P51157
Secondary accession number(s): Q8IYR8, Q8NI05

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 24, 2006
Last modified:	January 25, 2012
This is version 113 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.