P51157 (RAB28_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-28

Gene names

Name:

RAB28

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	221 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Tissue specificity	Isoform S is detected in most tissues investigated: cortex, liver, kidney, skeletal muscle, adipose tissue, testis and urothelium. Isoform L is predominantly expressed in testis.
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Nucleotide-binding
PTM	Acetylation Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GDP binding Inferred from direct assay Ref.8PubMed 20937701. Source: UniProtKB GTP binding Inferred from direct assay Ref.8. Source: UniProtKB GTPase activity Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform S (identifier: P51157-1)

Also known as: Rab28S;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform L (identifier: P51157-2)

Also known as: Rab28L;

The sequence of this isoform differs from the canonical sequence as follows:
     193-221: VVKADIVNYNQEPMSRTVNPPRSSMCAVQ → IVRAEIVKYPEEENQHTTSTQSRICSVQ

Isoform 3 (identifier: P51157-3)

The sequence of this isoform differs from the canonical sequence as follows:
     192-221: RVVKADIVNYNQEPMSRTVNPPRSSMCAVQ → GHFIIFISSTNRE

Note: No experimental confirmation available.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.7

Chain

2 – 218

217

Ras-related protein Rab-28

PRO_0000121227

Propeptide

219 – 221

3

Removed in mature form Probable

PRO_0000396721

Regions

Nucleotide binding

19 – 27

9

GTP

Nucleotide binding

68 – 72

5

GTP

Nucleotide binding

129 – 132

4

GTP

Nucleotide binding

159 – 161

3

GTP

Motif

41 – 49

9

Effector region By similarity

Amino acid modifications

Modified residue

2

1

N-acetylserine Ref.7

Modified residue

8

1

Phosphoserine Ref.7

Modified residue

218

1

Cysteine methyl ester Probable

Lipidation

218

1

S-farnesyl cysteine Ref.5

Natural variations

Alternative sequence

192 – 221

30

RVVKA…MCAVQ → GHFIIFISSTNRE in isoform 3.

VSP_045807

Alternative sequence

193 – 221

29

VVKAD…MCAVQ → IVRAEIVKYPEEENQHTTST QSRICSVQ in isoform L.

VSP_005530

Experimental info

Sequence conflict

22

1

A → T in CAA64364. Ref.1

Secondary structure

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221

Helix Strand Turn

Details...

Beta strand

12 – 18

7

Helix

25 – 33

9

Helix

34 – 36

3

Helix

39 – 43

5

Turn

44 – 46

3

Beta strand

47 – 56

10

Turn

57 – 59

3

Beta strand

60 – 68

9

Helix

71 – 75

5

Helix

79 – 83

5

Beta strand

87 – 94

8

Helix

98 – 102

5

Helix

104 – 118

15

Beta strand

123 – 129

7

Helix

131 – 136

6

Helix

141 – 151

11

Beta strand

154 – 158

5

Turn

160 – 162

3

Helix

166 – 177

12

Sequences

	Sequence		Length	Mass (Da)
	Isoform S (Rab28S) [UniParc]. Last modified January 24, 2006. Version 2. Checksum: 65AC9D6F10491916	FASTA	221	24,841
	10 20 30 40 50 60 MSDSEEESQD RQLKIVVLGD GASGKTSLTT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL 70 80 90 100 110 120 NVTLQIWDIG GQTIGGKMLD KYIYGAQGVL LVYDITNYQS FENLEDWYTV VKKVSEESET 130 140 150 160 170 180 QPLVALVGNK IDLEHMRTIK PEKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI 190 200 210 220 KLNKAEIEQS QRVVKADIVN YNQEPMSRTV NPPRSSMCAV Q
	Isoform L (Rab28L) [UniParc]. Checksum: 693F137237519E71	FASTA	220	24,853
	10 20 30 40 50 60 MSDSEEESQD RQLKIVVLGD GASGKTSLTT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL 70 80 90 100 110 120 NVTLQIWDIG GQTIGGKMLD KYIYGAQGVL LVYDITNYQS FENLEDWYTV VKKVSEESET 130 140 150 160 170 180 QPLVALVGNK IDLEHMRTIK PEKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI 190 200 210 220 KLNKAEIEQS QRIVRAEIVK YPEEENQHTT STQSRICSVQ
	Isoform 3 [UniParc]. Checksum: 3533DB9E6E6FD456	FASTA	204	22,971
	10 20 30 40 50 60 MSDSEEESQD RQLKIVVLGD GASGKTSLTT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL 70 80 90 100 110 120 NVTLQIWDIG GQTIGGKMLD KYIYGAQGVL LVYDITNYQS FENLEDWYTV VKKVSEESET 130 140 150 160 170 180 QPLVALVGNK IDLEHMRTIK PEKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI 190 200 KLNKAEIEQS QGHFIIFISS TNRE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Alternative mRNA splicing of the novel GTPase Rab28 generates isoforms with different C-termini." Brauers A., Schuermann A., Massmann S., Muehl-Zuerbes P., Becker W., Kainulainen H., Lie C., Joost H.-G. Eur. J. Biochem. 237:833-840(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L AND S). Tissue: Testis.
[2]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM L). Tissue: Brain.
[3]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS S AND 3). Tissue: Brain and Embryonic stem cell.
[5]	"Towards complete sets of farnesylated and geranylgeranylated proteins." Maurer-Stroh S., Koranda M., Benetka W., Schneider G., Sirota F.L., Eisenhaber F. PLoS Comput. Biol. 3:634-648(2007) [PubMed] [Europe PMC] [Abstract] Cited for: ISOPRENYLATION AT CYS-218.
[6]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]	"System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation." Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B. Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-8, MASS SPECTROMETRY, CLEAVAGE OF INITIATOR METHIONINE.
[8]	"Large nucleotide-dependent conformational change in Rab28." Lee S.H., Baek K., Dominguez R. FEBS Lett. 582:4107-4111(2008) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.1 ANGSTROMS) OF 11-184 IN COMPLEX WITH GTP AND GDP ANALOGS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X94703 mRNA. Translation: CAA64364.1.
AF498955 mRNA. Translation: AAM21103.1.
AC006226 Genomic DNA. No translation available.
AC006445 Genomic DNA. No translation available.
AC020729 Genomic DNA. No translation available.
BC035054 mRNA. Translation: AAH35054.1.
CX165950 mRNA. No translation available.

IPI

IPI00016377.
IPI00219679.

PIR

S65477.
S72399.

RefSeq

NP_001017979.1. NM_001017979.2.
NP_001153073.1. NM_001159601.1.
NP_004240.2. NM_004249.3.

UniGene

Hs.656060.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2HXS	X-ray	1.10	A	11-184	[»]
3E5H	X-ray	1.50	A	11-184	[»]

ProteinModelPortal

P51157.

ModBase

Search...

PTM databases

PhosphoSite

P51157.

Polymorphism databases

DMDM

85700393.

Proteomic databases

PaxDb

P51157.

PRIDE

P51157.

Protocols and materials databases

DNASU

9364.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000288723; ENSP00000288723; ENSG00000157869.
ENST00000330852; ENSP00000328551; ENSG00000157869.
ENST00000338176; ENSP00000340079; ENSG00000157869.

GeneID

9364.

KEGG

hsa:9364.

UCSC

uc003gmt.2. human.
uc003gmu.2. human.

Organism-specific databases

CTD

9364.

GeneCards

GC04M013369.

HGNC

HGNC:9768. RAB28.

HPA

HPA044575.

MIM

612994. gene.

neXtProt

NX_P51157.

PharmGKB

PA34119.

GenAtlas

Search...

Phylogenomic databases

eggNOG

COG1100.

HOGENOM

HOG000233968.

HOVERGEN

HBG101090.

InParanoid

P51157.

KO

K07915.

OMA

CFQRIAA.

OrthoDB

EOG43R3NJ.

PhylomeDB

P51157.

Gene expression databases

ArrayExpress

P51157.

Bgee

P51157.

CleanEx

HS_RAB28.

Genevestigator

P51157.

GermOnline

ENSG00000157869. Homo sapiens.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P51157.

GenomeRNAi

9364.

NextBio

35067.

SOURCE

Search...

Entry information

Entry name

RAB28_HUMAN

Accession

Primary (citable) accession number: P51157
Secondary accession number(s): G8JLC5, Q8IYR8, Q8NI05

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 24, 2006
Last modified:	May 1, 2013
This is version 125 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.