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Reviewed, UniProtKB/Swiss-Prot P51157 (RAB28_HUMAN)

Last modified November 25, 2008. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Ras-related protein Rab-28
Alternative name(s):
    Rab-26
Gene names
Name: RAB28
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length221 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Subcellular location

Cell membrane; Lipid-anchor; Cytoplasmic sidePotential.

Tissue specificity

Isoform S is detected in most tissues investigated (cortex, liver, kidney, skeletal muscle, adipose tissue, testis and urothelium), whereas isoform L is predominant in testis.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords

   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   LigandGTP-binding
Nucleotide-binding
   PTMLipoprotein
Prenylation
   Technical term3D-structure

Gene Ontology (GO)

   Biological processsmall GTPase mediated signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular componentplasma membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionGTP binding

Inferred from electronic annotation. Source: InterPro

GTPase activity Ref.1

Traceable author statement. Source: ProtInc

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform S (identifier: P51157-1)

Also known as: Rab28S;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform L (identifier: P51157-2)

Also known as: Rab28L;

The sequence of this isoform differs from the canonical sequence as follows:
     193-221: VVKADIVNYNQEPMSRTVNPPRSSMCAVQ → IVRAEIVKYPEEENQHTTSTQSRICSVQ

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 221221Ras-related protein Rab-28
PRO_0000121227

Regions

Nucleotide binding19 – 268GTP By similarity
Nucleotide binding68 – 725GTP By similarity
Nucleotide binding129 – 1324GTP By similarity
Motif41 – 499Effector region By similarity

Amino acid modifications

Lipidation2181S-farnesyl cysteine

Natural variations

Alternative sequence193 – 22129VVKAD…MCAVQ → IVRAEIVKYPEEENQHTTST QSRICSVQ in isoform L.
VSP_005530

Experimental info

Sequence conflict221A → T in CAA64364. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform S (Rab28S) [UniParc].

Last modified January 24, 2006. Version 2.
Checksum: 65AC9D6F10491916

FASTA22124,841
        10         20         30         40         50         60 
MSDSEEESQD RQLKIVVLGD GASGKTSLTT CFAQETFGKQ YKQTIGLDFF LRRITLPGNL 

        70         80         90        100        110        120 
NVTLQIWDIG GQTIGGKMLD KYIYGAQGVL LVYDITNYQS FENLEDWYTV VKKVSEESET 

       130        140        150        160        170        180 
QPLVALVGNK IDLEHMRTIK PEKHLRFCQE NGFSSHFVSA KTGDSVFLCF QKVAAEILGI 

       190        200        210        220 
KLNKAEIEQS QRVVKADIVN YNQEPMSRTV NPPRSSMCAV Q 

« Hide

Isoform L (Rab28L) [UniParc].

Checksum: 693F137237519E71
Show »

22024,853

References

« Hide 'large scale' references
[1]"Alternative mRNA splicing of the novel GTPase Rab28 generates isoforms with different C-termini."
Brauers A., Schuermann A., Massmann S., Muehl-Zuerbes P., Becker W., Kainulainen H., Lie C., Joost H.-G.
Eur. J. Biochem. 237:833-840(1996) [PubMed: 8647132] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS L AND S).
Tissue: Testis.
[2]"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)."
Puhl H.L. III, Ikeda S.R., Aronstam R.S.
Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM L).
Tissue: Brain.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[4]"Towards complete sets of farnesylated and geranylgeranylated proteins."
Maurer-Stroh S., Koranda M., Benetka W., Schneider G., Sirota F.L., Eisenhaber F.
PLoS Comput. Biol. 3:634-648(2007) [PubMed: 17411337] [Abstract]
Cited for: ISOPRENYLATION AT CYS-218.

Cross-references

Sequence databases

X94703 mRNA. Translation: CAA64364.1.
AF498955 mRNA. Translation: AAM21103.1.
BC035054 mRNA. Translation: AAH35054.1.
PIRS65477.
S72399.
RefSeqNP_001017979.1.
NP_004240.1.
UniGeneHs.656060

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2HXSX-ray1.10A8-184[»]
2HY4X-ray1.50A8-184[»]
3E5HX-ray1.50A8-184[»]
ModBaseSearch...

Genome annotation databases

EnsemblENSG00000157869. Homo sapiens. [Contig view]
GeneID9364.
KEGGhsa:9364.

Organism-specific databases

H-InvDBHIX0056321.
HGNCHGNC:9768. RAB28.
PharmGKBPA34119.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMP51157.
HOVERGENP51157.

Gene expression databases

ArrayExpressP51157.
CleanExHS_RAB28.
GermOnlineENSG00000157869. Homo sapiens.

Family and domain databases

InterProIPR013753. Ras.
IPR001806. Ras_trnsfrmng.
IPR005225. Small_GTP_bd.
[Graphical view]
PfamPF00071. Ras. 1 hit.
[Graphical view]
PRINTSPR00449. RASTRNSFRMNG.
TIGRFAMsTIGR00231. small_GTP. 1 hit.
ProtoNetSearch...

Other Resources

LinkHubP51157.
NextBio35067.

Entry information

Entry nameRAB28_HUMAN
AccessionPrimary (citable) accession number: P51157
Secondary accession number(s): Q8IYR8, Q8NI05
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 24, 2006
Last modified: November 25, 2008
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 4

Human chromosome 4: entries, gene names and cross-references to MIM

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents