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Protein

Ras-related protein Rab-7a

Gene

Rab7a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation (By similarity). Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (PubMed:23708524). Required for the exosomal release of SDCBP, CD63 and syndecan (By similarity).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi15 – 22GTPBy similarity8
Nucleotide bindingi34 – 40GTPBy similarity7
Nucleotide bindingi63 – 67GTPBy similarity5
Nucleotide bindingi125 – 128GTPBy similarity4
Nucleotide bindingi156 – 157GTPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Autophagy, Lipid degradation, Lipid metabolism, Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-7a
Gene namesi
Name:Rab7a
Synonyms:Rab7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:105068. Rab7.

Subcellular locationi

GO - Cellular componenti

  • alveolar lamellar body Source: Ensembl
  • autophagosome membrane Source: UniProtKB-SubCell
  • cytoplasm Source: MGI
  • cytosol Source: GOC
  • endosome membrane Source: MGI
  • extracellular exosome Source: MGI
  • extrinsic component of lysosome membrane Source: GO_Central
  • Golgi apparatus Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • late endosome Source: MGI
  • late endosome membrane Source: GO_Central
  • lipid particle Source: GO_Central
  • lysosome Source: MGI
  • melanosome membrane Source: UniProtKB-SubCell
  • phagocytic vesicle Source: UniProtKB
  • phagocytic vesicle membrane Source: UniProtKB-SubCell
  • pre-autophagosomal structure membrane Source: UniProtKB
  • retromer complex Source: Ensembl
  • terminal bouton Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lipid droplet, Lysosome, Membrane

Pathology & Biotechi

Disruption phenotypei

Reduces both the basal and, to a greater degree, agonist-stimulated glycerol releases. the ADRB2-stimulated liposlysis.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001211222 – 207Ras-related protein Rab-7aAdd BLAST206

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineBy similarity1
Modified residuei72PhosphoserineCombined sources1
Lipidationi205S-geranylgeranyl cysteineBy similarity1
Modified residuei207Cysteine methyl esterBy similarity1
Lipidationi207S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP51150.
PaxDbiP51150.
PeptideAtlasiP51150.
PRIDEiP51150.

PTM databases

iPTMnetiP51150.
PhosphoSitePlusiP51150.
SwissPalmiP51150.

Expressioni

Tissue specificityi

Widely expressed. High expression in liver, heart and kidney. Found in sensory and motor neurons.1 Publication

Gene expression databases

BgeeiENSMUSG00000079477.
CleanExiMM_RAB7.
ExpressionAtlasiP51150. baseline and differential.
GenevisibleiP51150. MM.

Interactioni

Subunit structurei

Interacts with NTRK1/TRKA (By similarity), RILP (By similarity), PSMA7 (By similarity), RNF115 (PubMed:12972561) and FYCO1 (By similarity). Interacts with the PIK3C3/VPS34-PIK3R4 complex (By similarity). The GTP-bound form interacts with OSBPL1A and RAC1 (By similarity). Interacts with CLN3 (By similarity). Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (By similarity). Interacts with C9orf72 (PubMed:24549040). Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4) (PubMed:20048159). Interacts with CLN5 (By similarity).By similarity3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi202553. 9 interactors.
DIPiDIP-60513N.
IntActiP51150. 11 interactors.
MINTiMINT-1864693.
STRINGi10090.ENSMUSP00000092658.

Structurei

3D structure databases

ProteinModelPortaliP51150.
SMRiP51150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi37 – 45Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP51150.
KOiK07897.
OMAiDYPDPIK.
OrthoDBiEOG091G0JIV.
PhylomeDBiP51150.
TreeFamiTF105605.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P51150-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV
60 70 80 90 100
MVDDRLVTMQ IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD
110 120 130 140 150
SWRDEFLIQA SPRDPENFPF VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP
160 170 180 190 200
YFETSAKEAI NVEQAFQTIA RNALKQETEV ELYNEFPEPI KLDKNDRAKA

SAESCSC
Length:207
Mass (Da):23,490
Last modified:April 13, 2004 - v2
Checksum:iA2AF33B16A672971
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti145S → R in CAA61797 (PubMed:8547311).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89650 mRNA. Translation: CAA61797.1.
AK005004 mRNA. Translation: BAB23738.1.
BC004597 mRNA. Translation: AAH04597.1.
BC086793 mRNA. Translation: AAH86793.1.
CCDSiCCDS39552.1.
PIRiS62733.
RefSeqiNP_001280581.1. NM_001293652.1.
NP_001280582.1. NM_001293653.1.
NP_001280583.1. NM_001293654.1.
NP_001280584.1. NM_001293655.1.
NP_033031.2. NM_009005.3.
UniGeneiMm.333233.
Mm.471618.
Mm.486309.

Genome annotation databases

EnsembliENSMUST00000095048; ENSMUSP00000092658; ENSMUSG00000079477.
ENSMUST00000113596; ENSMUSP00000109226; ENSMUSG00000079477.
ENSMUST00000113597; ENSMUSP00000109227; ENSMUSG00000079477.
ENSMUST00000113598; ENSMUSP00000109228; ENSMUSG00000079477.
ENSMUST00000113600; ENSMUSP00000109230; ENSMUSG00000079477.
GeneIDi19349.
KEGGimmu:19349.
UCSCiuc009cuv.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X89650 mRNA. Translation: CAA61797.1.
AK005004 mRNA. Translation: BAB23738.1.
BC004597 mRNA. Translation: AAH04597.1.
BC086793 mRNA. Translation: AAH86793.1.
CCDSiCCDS39552.1.
PIRiS62733.
RefSeqiNP_001280581.1. NM_001293652.1.
NP_001280582.1. NM_001293653.1.
NP_001280583.1. NM_001293654.1.
NP_001280584.1. NM_001293655.1.
NP_033031.2. NM_009005.3.
UniGeneiMm.333233.
Mm.471618.
Mm.486309.

3D structure databases

ProteinModelPortaliP51150.
SMRiP51150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202553. 9 interactors.
DIPiDIP-60513N.
IntActiP51150. 11 interactors.
MINTiMINT-1864693.
STRINGi10090.ENSMUSP00000092658.

PTM databases

iPTMnetiP51150.
PhosphoSitePlusiP51150.
SwissPalmiP51150.

Proteomic databases

EPDiP51150.
PaxDbiP51150.
PeptideAtlasiP51150.
PRIDEiP51150.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000095048; ENSMUSP00000092658; ENSMUSG00000079477.
ENSMUST00000113596; ENSMUSP00000109226; ENSMUSG00000079477.
ENSMUST00000113597; ENSMUSP00000109227; ENSMUSG00000079477.
ENSMUST00000113598; ENSMUSP00000109228; ENSMUSG00000079477.
ENSMUST00000113600; ENSMUSP00000109230; ENSMUSG00000079477.
GeneIDi19349.
KEGGimmu:19349.
UCSCiuc009cuv.2. mouse.

Organism-specific databases

CTDi19349.
MGIiMGI:105068. Rab7.

Phylogenomic databases

eggNOGiKOG0394. Eukaryota.
ENOG410XNZV. LUCA.
GeneTreeiENSGT00760000119125.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP51150.
KOiK07897.
OMAiDYPDPIK.
OrthoDBiEOG091G0JIV.
PhylomeDBiP51150.
TreeFamiTF105605.

Enzyme and pathway databases

ReactomeiR-MMU-2132295. MHC class II antigen presentation.
R-MMU-6798695. Neutrophil degranulation.
R-MMU-8876198. RAB GEFs exchange GTP for GDP on RABs.

Miscellaneous databases

PROiP51150.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000079477.
CleanExiMM_RAB7.
ExpressionAtlasiP51150. baseline and differential.
GenevisibleiP51150. MM.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAB7A_MOUSE
AccessioniPrimary (citable) accession number: P51150
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: April 13, 2004
Last modified: November 30, 2016
This is version 154 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.