P51145 (FOSL2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fos-related antigen 2
Short name=FRA-2

Gene names

Name:	Fosl2
Synonyms:	Fra-2, Fra2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Controls osteoclast survival and size By similarity. As a dimer with JUN, activates LIF transcription By similarity. Activates CEBPB transcription in PGE2-activated osteoblasts. Ref.2
Subunit structure	Heterodimer By similarity.
Subcellular location	Nucleus Ref.2.
Tissue specificity	Expressed in the brain cortex. Expressed at night in pineal gland (at protein level). Also expressed in osteoblasts (at protein level). Ref.1 Ref.2 Ref.3
Induction	In the pineal gland, exhibits night/day variations with a 4-fold increased expression at night (at protein level). Up-regulation is due to a large degree to the release of norepinephrine from nerve terminals in the pineal gland and cAMP signaling pathway. Nocturnally elevated expression is also observed in the brain cortex. In osteoblasts, up-regulated by PGE2 (at protein level). Ref.1 Ref.2 Ref.3
Sequence similarities	Belongs to the bZIP family. Fos subfamily. Contains 1 bZIP (basic-leucine zipper) domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 11389931. Source: RGD circadian rhythm Inferred from expression pattern PubMed 10987819. Source: RGD conditioned taste aversion Inferred from expression pattern PubMed 18374904. Source: RGD female pregnancy Inferred from expression pattern PubMed 12080023. Source: RGD photoperiodism Inferred from expression pattern PubMed 16962714. Source: RGD positive regulation of transcription, DNA-dependent Inferred from direct assay PubMed 11340164. Source: RGD response to cAMP Inferred from expression pattern PubMed 18280640. Source: RGD response to cold Inferred from expression pattern PubMed 16169632. Source: RGD response to cytokine stimulus Inferred from expression pattern PubMed 18280640. Source: RGD response to estradiol stimulus Inferred from mutant phenotype PubMed 12021174. Source: RGD response to hypoxia Inferred from mutant phenotype PubMed 15892448. Source: RGD response to mechanical stimulus Inferred from expression pattern PubMed 16373449. Source: RGD response to peptide hormone stimulus Inferred from expression pattern PubMed 10934195. Source: RGD response to progesterone stimulus Inferred from expression pattern PubMed 12080023. Source: RGD response to toxin Inferred from expression pattern PubMed 11295234. Source: RGD sleep Inferred from expression pattern PubMed 16690212. Source: RGD transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 10830307. Source: RGD
Cellular_component	nucleus Inferred from direct assay PubMed 10830307. Source: RGD
Molecular_function	double-stranded DNA binding Inferred from direct assay PubMed 10830307. Source: RGD protein heterodimerization activity Inferred by curator PubMed 10830307. Source: RGD sequence-specific DNA binding Inferred from direct assay PubMed 10830307. Source: RGD sequence-specific DNA binding transcription factor activity Inferred from mutant phenotype PubMed 10830307. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Fos-related antigen 2		PRO_0000076485
Regions
	Domain	124 – 187	64	bZIP
	Region	126 – 128	3	Basic motif By similarity
	Region	129 – 136	8	Leucine-zipper By similarity
Amino acid modifications
	Modified residue	120	1	Phosphoserine By similarity
	Modified residue	200	1	Phosphoserine By similarity
	Modified residue	230	1	Phosphoserine By similarity
	Modified residue	320	1	Phosphoserine By similarity
Experimental info
	Sequence conflict	56	1	T → TT in AAC52310. Ref.1
	Sequence conflict	89	1	A → R in AAC52310. Ref.1
	Sequence conflict	154	1	A → T in AAC52310. Ref.1
	Sequence conflict	201	1	P → S in AAC52310. Ref.1
	Sequence conflict	206	1	L → V in AAC52310. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P51145 [UniParc]. Last modified December 14, 2011. Version 2. Checksum: 07DD44D47D0B790A	FASTA	326	35,255
	10 20 30 40 50 60 MYQDYPGNFD TSSRGSSGSP AHAESYSSGG GGQQKFRVDM PGSGSAFIPT INAITTSQDL 70 80 90 100 110 120 QWMVQPTVIT SMSNPYPRSH PYSPLPGLAS VPGHMALPRP GVIKTIGTTV GRRRRDEQLS 130 140 150 160 170 180 PEEEEKRRIR RERNKLAAAK CRNRRRELTE KLQAETEELE EEKSGLQKEI AELQKEKEKL 190 200 210 220 230 240 EFMLVAHGPV CKISPEERRS PPTSGLQSLR GTGSAVGPVV VKQEPPEEDS PSSSAGMDKT 250 260 270 280 290 300 QRSVIKPISI AGGGFYGEEP LHTPIVVTST PAITPGTSNL VFTYPSVLEQ ESPASPSESC 310 320 SKAHRRSSSS GDQSSDSLNS PTLLAL

References

[1]	"Circadian expression of transcription factor Fra-2 in the rat pineal gland." Baler R.D., Klein D.C. J. Biol. Chem. 270:27319-27325(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION. Strain: Sprague-Dawley. Tissue: Pineal gland.
[2]	"Fos-related antigen 2 controls protein kinase A-induced CCAAT/enhancer-binding protein beta expression in osteoblasts." Chang W., Rewari A., Centrella M., McCarthy T.L. J. Biol. Chem. 279:42438-42444(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION. Strain: Sprague-Dawley. Tissue: Osteoblast.
[3]	"Night/day changes in pineal expression of >600 genes: central role of adrenergic/cAMP signaling." Bailey M.J., Coon S.L., Carter D.A., Humphries A., Kim J.S., Shi Q., Gaildrat P., Morin F., Ganguly S., Hogenesch J.B., Weller J.L., Rath M.F., Moller M., Baler R., Sugden D., Rangel Z.G., Munson P.J., Klein D.C. J. Biol. Chem. 284:7606-7622(2009) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY, INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U18913 mRNA. Translation: AAC52310.1. AY622611 mRNA. Translation: AAT52165.1.
IPI	IPI00325210.
PIR	I55459.
RefSeq	NP_037086.1. NM_012954.1.
UniGene	Rn.10962.
3D structure databases
ProteinModelPortal	P51145.
SMR	P51145. Positions 126-184.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	25446.
KEGG	rno:25446.
Organism-specific databases
CTD	2355.
RGD	2628. Fosl2.
Phylogenomic databases
HOVERGEN	HBG005743.
KO	K09030.
Gene expression databases
Genevestigator	P51145.
Family and domain databases
InterPro	IPR004827. bZIP. IPR000837. Leuzip_Fos. [Graphical view]
Pfam	PF00170. bZIP_1. 1 hit. [Graphical view]
PRINTS	PR00042. LEUZIPPRFOS.
SMART	SM00338. BRLZ. 1 hit. [Graphical view]
PROSITE	PS50217. BZIP. 1 hit. PS00036. BZIP_BASIC. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	606679.

Entry information

Entry name

FOSL2_RAT

Accession

Primary (citable) accession number: P51145
Secondary accession number(s): Q6GXE4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 14, 2011
Last modified:	April 3, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program