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P51140 (DSH_DROME) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 132. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Segment polarity protein dishevelled
Gene names
Name:dsh
ORF Names:CG18361
OrganismDrosophila melanogaster (Fruit fly) [Reference proteome]
Taxonomic identifier7227 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Protein attributes

Sequence length623 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required to establish coherent arrays of polarized cells and segments in embryos. Plays a role in wingless (wg) signaling, possibly through the reception of the wg signal by target cells and subsequent redistribution of arm protein in response to that signal in embryos. This signal seems to be required to establish planar cell polarity and identity. Ref.1 Ref.2 Ref.6

Subunit structure

Interacts with nkd. This interaction may require zinc. Ref.7 Ref.8 Ref.9

Subcellular location

Cytoplasm. Nucleus. Membrane; Peripheral membrane protein. Note: Associated with the membrane when hyperphosphorylated. Ref.6 Ref.9

Tissue specificity

Found in egg chambers of the ovary and ubiquitously throughout embryogenesis and in disks. Expression is not seen in salivary glands, muscles or ventral ganglia but is observed in brain lobes.

Developmental stage

Expressed at high levels at 0-1 hour and from 5-17 hours. Also expressed at high levels in early pupae. Ref.2

Post-translational modification

Phosphorylated. Wg signaling generates the hyperphosphorylated active forms. Ref.6

Sequence similarities

Belongs to the DSH family.

Contains 1 DEP domain.

Contains 1 DIX domain.

Contains 1 PDZ (DHR) domain.

Ontologies

Keywords
   Biological processWnt signaling pathway
   Cellular componentCytoplasm
Membrane
Nucleus
   Molecular functionDevelopmental protein
Segmentation polarity protein
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processR3/R4 cell fate commitment

Non-traceable author statement PubMed 12414186. Source: FlyBase

Wnt signaling pathway

Non-traceable author statement PubMed 12000787PubMed 12147138PubMed 12231350. Source: FlyBase

Wnt signaling pathway, planar cell polarity pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

axon extension

Inferred from mutant phenotype PubMed 17032066. Source: FlyBase

border follicle cell migration

Inferred from mutant phenotype PubMed 17652348. Source: FlyBase

cellular component movement

Inferred from mutant phenotype PubMed 11970894. Source: FlyBase

compound eye morphogenesis

Inferred from mutant phenotype PubMed 14602079. Source: FlyBase

dorsal closure

Inferred from mutant phenotype PubMed 12447392. Source: FlyBase

dorsal closure, elongation of leading edge cells

Inferred from mutant phenotype PubMed 12447392. Source: FlyBase

epidermis morphogenesis

Inferred from mutant phenotype PubMed 20736316. Source: FlyBase

establishment of epithelial cell apical/basal polarity

Inferred from mutant phenotype PubMed 12540853. Source: FlyBase

establishment of epithelial cell planar polarity

Inferred from mutant phenotype PubMed 20736316. Source: FlyBase

establishment of imaginal disc-derived wing hair orientation

Inferred from mutant phenotype PubMed 15075368PubMed 16824921. Source: FlyBase

establishment of ommatidial planar polarity

Inferred from mutant phenotype PubMed 7555730. Source: FlyBase

establishment of planar polarity

Traceable author statement PubMed 12121634PubMed 12414186PubMed 15475117. Source: FlyBase

establishment of thoracic bristle planar orientation

Traceable author statement PubMed 12137730. Source: FlyBase

establishment of tissue polarity

Non-traceable author statement PubMed 12000787. Source: FlyBase

female gonad development

Inferred from mutant phenotype PubMed 11970894. Source: FlyBase

filopodium assembly

Inferred from mutant phenotype PubMed 12447392. Source: FlyBase

heart development

Non-traceable author statement PubMed 12027431. Source: FlyBase

heart formation

Inferred from mutant phenotype PubMed 8660881. Source: FlyBase

imaginal disc-derived leg joint morphogenesis

Inferred from mutant phenotype PubMed 22736244. Source: FlyBase

imaginal disc-derived wing hair organization

Inferred from mutant phenotype PubMed 15075368. Source: FlyBase

imaginal disc-derived wing morphogenesis

Traceable author statement PubMed 12127767. Source: FlyBase

intracellular signal transduction

Inferred from electronic annotation. Source: InterPro

lamellipodium assembly

Inferred from mutant phenotype PubMed 12447392. Source: FlyBase

motor neuron axon guidance

Inferred from mutant phenotype PubMed 17764943. Source: FlyBase

negative regulation of Notch signaling pathway

Inferred from mutant phenotype PubMed 22736244. Source: FlyBase

negative regulation of heart induction by canonical Wnt signaling pathway

Inferred from mutant phenotype PubMed 8660881. Source: FlyBase

ommatidial rotation

Non-traceable author statement PubMed 12000787. Source: FlyBase

oogenesis

Inferred from mutant phenotype PubMed 12783796. Source: FlyBase

ovarian follicle cell development

Inferred from mutant phenotype PubMed 16256740PubMed 16542414. Source: FlyBase

protein localization

Traceable author statement PubMed 12127767. Source: FlyBase

regulation of actin filament bundle assembly

Inferred from genetic interaction PubMed 11301004. Source: FlyBase

regulation of compound eye pigmentation

Inferred from mutant phenotype PubMed 16914498. Source: FlyBase

regulation of tube length, open tracheal system

Inferred from mutant phenotype PubMed 19956736. Source: FlyBase

retinal ganglion cell axon guidance

Inferred from mutant phenotype PubMed 16369482. Source: FlyBase

salivary gland morphogenesis

Inferred from mutant phenotype PubMed 17507403. Source: FlyBase

segment polarity determination

Inferred from electronic annotation. Source: UniProtKB-KW

sensory organ development

Non-traceable author statement PubMed 11932008. Source: FlyBase

somatic stem cell maintenance

Inferred from mutant phenotype PubMed 12783796PubMed 16256740. Source: FlyBase

synaptic target inhibition

Inferred from mutant phenotype PubMed 17764943. Source: FlyBase

wing and notum subfield formation

Inferred from mutant phenotype PubMed 16414040. Source: FlyBase

   Cellular_componentadherens junction

Traceable author statement PubMed 12000787. Source: FlyBase

cell cortex

Inferred from Biological aspect of Ancestor. Source: RefGenome

cytoplasm

Traceable author statement PubMed 12000787. Source: FlyBase

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from direct assay PubMed 16824921. Source: FlyBase

   Molecular_functionNotch binding

Inferred from physical interaction PubMed 22736244. Source: FlyBase

phosphatidic acid binding

Inferred from direct assay PubMed 19234454. Source: FlyBase

protein domain specific binding

Inferred from physical interaction PubMed 11356022. Source: FlyBase

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 623623Segment polarity protein dishevelled
PRO_0000145740

Regions

Domain8 – 9184DIX
Domain252 – 32473PDZ
Domain404 – 47875DEP
Region166 – 623458Interaction with nkd
Compositional bias119 – 14628Poly-Gln
Compositional bias154 – 1596Poly-Gln
Compositional bias551 – 5577Poly-Ser
Compositional bias599 – 60810Poly-Gly

Experimental info

Sequence conflict2761D → N in AAA20216. Ref.1
Sequence conflict5651I → T in AAA16535. Ref.2
Sequence conflict6191F → S in AAA16535. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P51140 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: 0BA24ED350666CF5

FASTA62368,917
        10         20         30         40         50         60 
MDADRGGGQE TKVIYHIDDE TTPYLVKIPI PSAQVTLRDF KLVLNKQNNN YKYFFKSMDA 

        70         80         90        100        110        120 
DFGVVKEEIA DDSTILPCFN GRVVSWLVSA DGTNQSDNCS ELPTSECELG MGLTNRKLQQ 

       130        140        150        160        170        180 
QQQQHQQQQQ QQQQQHQQQQ QQQQQQVQPV QLAQQQQQQV LHHQKMMGNP LLQPPPLTYQ 

       190        200        210        220        230        240 
SASVLSSDLD STSLFGTESE LTLDRDMTDY SSVQRLQVRK KPQRRKKRAP SMSRTSSYSS 

       250        260        270        280        290        300 
ITDSTMSLNI ITVSINMEAV NFLGISIVGQ SNRGGDGGIY VGSIMKGGAV ALDGRIEPGD 

       310        320        330        340        350        360 
MILQVNDVNF ENMTNDEAVR VLREVVQKPG PIKLVVAKCW DPNPKGYFTI PRTEPVRPID 

       370        380        390        400        410        420 
PGAWVAHTQA LTSHDSIIAD IAEPIKERLD QNNLEEIVKA MTKPDSGLEI RDRMWLKITI 

       430        440        450        460        470        480 
PNAFIGADAV NWVLENVEDV QDRREARRIV SAMLRSNYIK HTVNKLTFSE QCYYVVNEER 

       490        500        510        520        530        540 
NPNLLGRGHL HPHQLPHGHG GHALSHADTE SITSDIGPLP NPPIYMPYSA TYNPSHGYQP 

       550        560        570        580        590        600 
IQYGIAERHI SSGSSSSDVL TSKDISASQS DITSVIHQAN QLTIAAHGSN KSSGSSNRGG 

       610        620 
GGGGGGGGNN TNDQDVSVFN YVL 

« Hide

References

« Hide 'large scale' references
[1]"Dishevelled is required during wingless signaling to establish both cell polarity and cell identity."
Theisen H., Purcell J., Bennett M., Kansagara D., Syed A., Marsh J.L.
Development 120:347-360(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"The Drosophila segment polarity gene dishevelled encodes a novel protein required for response to the wingless signal."
Klingensmith J., Nusse R., Perrimon N.
Genes Dev. 8:118-130(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
[3]"The genome sequence of Drosophila melanogaster."
Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. expand/collapse author list , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Berkeley.
[4]"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review."
Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. expand/collapse author list , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.
Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: Berkeley.
[5]Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Berkeley.
Tissue: Embryo.
[6]"The dishevelled protein is modified by wingless signaling in Drosophila."
Yanagawa S., van Leeuwen F., Wodarz A., Klingensmith J., Nusse R.
Genes Dev. 9:1087-1097(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
[7]"Naked cuticle targets dishevelled to antagonize Wnt signal transduction."
Rousset R., Mack J.A., Wharton K.A. Jr., Axelrod J.D., Cadigan K.M., Fish M.P., Nusse R., Scott M.P.
Genes Dev. 15:658-671(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NKD.
[8]"Zinc-dependent interaction between dishevelled and the Drosophila Wnt antagonist naked cuticle."
Rousset R., Wharton K.A. Jr., Zimmermann G., Scott M.P.
J. Biol. Chem. 277:49019-49026(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NKD.
[9]"An unconventional nuclear localization motif is crucial for function of the Drosophila Wnt/wingless antagonist Naked cuticle."
Waldrop S., Chan C.-C., Cagatay T., Zhang S., Rousset R., Mack J.A., Zeng W., Fish M.P., Zhang M., Amanai M., Wharton K.A. Jr.
Genetics 174:331-348(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH NKD, SUBCELLULAR LOCATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U02491 mRNA. Translation: AAA20216.1.
L26974 mRNA. Translation: AAA16535.1.
AE014298 Genomic DNA. Translation: AAF48033.1.
BT023781 mRNA. Translation: AAZ41790.1.
PIRA49840.
RefSeqNP_511118.2. NM_078563.3.
UniGeneDm.2927.

3D structure databases

ProteinModelPortalP51140.
SMRP51140. Positions 10-88, 250-338, 391-503.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid58490. 81 interactions.
IntActP51140. 16 interactions.
MINTMINT-8291181.

Proteomic databases

PaxDbP51140.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaFBtr0073454; FBpp0073310; FBgn0000499.
GeneID32078.
KEGGdme:Dmel_CG18361.
UCSCCG18361-RA. d. melanogaster.

Organism-specific databases

CTD32078.
FlyBaseFBgn0000499. dsh.

Phylogenomic databases

eggNOGNOG322275.
GeneTreeENSGT00390000013552.
InParanoidP51140.
KOK02353.
OMAIHQANQL.
OrthoDBEOG7BP82N.
PhylomeDBP51140.

Enzyme and pathway databases

SignaLinkP51140.

Gene expression databases

BgeeP51140.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.30.42.10. 1 hit.
InterProIPR000591. DEP_dom.
IPR008339. Dishevelled_fam.
IPR003351. Dishevelled_protein_dom.
IPR001158. DIX.
IPR015506. Dsh/Dvl-rel.
IPR001478. PDZ.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR10878. PTHR10878. 1 hit.
PfamPF00610. DEP. 1 hit.
PF02377. Dishevelled. 1 hit.
PF00778. DIX. 1 hit.
PF00595. PDZ. 1 hit.
[Graphical view]
PRINTSPR01760. DISHEVELLED.
SMARTSM00021. DAX. 1 hit.
SM00049. DEP. 1 hit.
SM00228. PDZ. 1 hit.
[Graphical view]
SUPFAMSSF50156. SSF50156. 1 hit.
PROSITEPS50186. DEP. 1 hit.
PS50841. DIX. 1 hit.
PS50106. PDZ. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GenomeRNAi32078.
NextBio776701.
PROP51140.

Entry information

Entry nameDSH_DROME
AccessionPrimary (citable) accession number: P51140
Secondary accession number(s): Q494J5, Q9VYZ9
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: June 21, 2005
Last modified: April 16, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase