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P51140

- DSH_DROME

UniProt

P51140 - DSH_DROME

Protein

Segment polarity protein dishevelled

Gene

dsh

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 137 (01 Oct 2014)
      Sequence version 2 (21 Jun 2005)
      Previous versions | rss
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    Functioni

    Required to establish coherent arrays of polarized cells and segments in embryos. Plays a role in wingless (wg) signaling, possibly through the reception of the wg signal by target cells and subsequent redistribution of arm protein in response to that signal in embryos. This signal seems to be required to establish planar cell polarity and identity.3 Publications

    GO - Molecular functioni

    1. Notch binding Source: FlyBase
    2. phosphatidic acid binding Source: FlyBase
    3. protein binding Source: UniProtKB
    4. protein domain specific binding Source: FlyBase
    5. signal transducer activity Source: InterPro

    GO - Biological processi

    1. axon extension Source: FlyBase
    2. axon guidance Source: FlyBase
    3. border follicle cell migration Source: FlyBase
    4. cellular component movement Source: FlyBase
    5. compound eye morphogenesis Source: FlyBase
    6. dorsal closure Source: FlyBase
    7. dorsal closure, elongation of leading edge cells Source: FlyBase
    8. epidermis morphogenesis Source: FlyBase
    9. establishment of epithelial cell apical/basal polarity Source: FlyBase
    10. establishment of epithelial cell planar polarity Source: FlyBase
    11. establishment of imaginal disc-derived wing hair orientation Source: FlyBase
    12. establishment of ommatidial planar polarity Source: FlyBase
    13. establishment of planar polarity Source: FlyBase
    14. establishment of thoracic bristle planar orientation Source: FlyBase
    15. establishment of tissue polarity Source: FlyBase
    16. female gonad development Source: FlyBase
    17. filopodium assembly Source: FlyBase
    18. heart development Source: FlyBase
    19. heart formation Source: FlyBase
    20. imaginal disc-derived leg joint morphogenesis Source: FlyBase
    21. imaginal disc-derived wing hair organization Source: FlyBase
    22. imaginal disc-derived wing morphogenesis Source: FlyBase
    23. intracellular signal transduction Source: InterPro
    24. lamellipodium assembly Source: FlyBase
    25. motor neuron axon guidance Source: FlyBase
    26. negative regulation of heart induction by canonical Wnt signaling pathway Source: FlyBase
    27. negative regulation of Notch signaling pathway Source: FlyBase
    28. ommatidial rotation Source: FlyBase
    29. oogenesis Source: FlyBase
    30. ovarian follicle cell development Source: FlyBase
    31. protein localization Source: FlyBase
    32. R3/R4 cell fate commitment Source: FlyBase
    33. regulation of actin filament bundle assembly Source: FlyBase
    34. regulation of compound eye pigmentation Source: FlyBase
    35. regulation of tube length, open tracheal system Source: FlyBase
    36. retinal ganglion cell axon guidance Source: FlyBase
    37. salivary gland morphogenesis Source: FlyBase
    38. segment polarity determination Source: UniProtKB-KW
    39. sensory organ development Source: FlyBase
    40. somatic stem cell maintenance Source: FlyBase
    41. synaptic target inhibition Source: FlyBase
    42. wing and notum subfield formation Source: FlyBase
    43. Wnt signaling pathway Source: FlyBase
    44. Wnt signaling pathway, planar cell polarity pathway Source: RefGenome

    Keywords - Molecular functioni

    Developmental protein, Segmentation polarity protein

    Keywords - Biological processi

    Wnt signaling pathway

    Enzyme and pathway databases

    ReactomeiREACT_207070. TCF dependent signaling in response to WNT.
    SignaLinkiP51140.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Segment polarity protein dishevelled
    Gene namesi
    Name:dsh
    ORF Names:CG18361
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome X

    Organism-specific databases

    FlyBaseiFBgn0000499. dsh.

    Subcellular locationi

    Cytoplasm. Nucleus. Membrane; Peripheral membrane protein
    Note: Associated with the membrane when hyperphosphorylated.

    GO - Cellular componenti

    1. adherens junction Source: FlyBase
    2. cell cortex Source: RefGenome
    3. cytoplasm Source: FlyBase
    4. nucleus Source: UniProtKB-SubCell
    5. plasma membrane Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 623623Segment polarity protein dishevelledPRO_0000145740Add
    BLAST

    Post-translational modificationi

    Phosphorylated. Wg signaling generates the hyperphosphorylated active forms.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP51140.

    Expressioni

    Tissue specificityi

    Found in egg chambers of the ovary and ubiquitously throughout embryogenesis and in disks. Expression is not seen in salivary glands, muscles or ventral ganglia but is observed in brain lobes.

    Developmental stagei

    Expressed at high levels at 0-1 hour and from 5-17 hours. Also expressed at high levels in early pupae.1 Publication

    Gene expression databases

    BgeeiP51140.

    Interactioni

    Subunit structurei

    Interacts with nkd. This interaction may require zinc.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    cnoQ242793EBI-499383,EBI-868783
    dgoQ7JUF25EBI-499383,EBI-1158635
    nkdQ9VVV910EBI-499383,EBI-125843
    Nkd1Q99MH62EBI-499383,EBI-1538321From a different organism.

    Protein-protein interaction databases

    BioGridi58490. 81 interactions.
    IntActiP51140. 16 interactions.
    MINTiMINT-8291181.

    Structurei

    3D structure databases

    ProteinModelPortaliP51140.
    SMRiP51140. Positions 10-88, 250-338, 391-478.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini8 – 9184DIXPROSITE-ProRule annotationAdd
    BLAST
    Domaini252 – 32473PDZPROSITE-ProRule annotationAdd
    BLAST
    Domaini404 – 47875DEPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni166 – 623458Interaction with nkdAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi119 – 14628Poly-GlnAdd
    BLAST
    Compositional biasi154 – 1596Poly-Gln
    Compositional biasi551 – 5577Poly-Ser
    Compositional biasi599 – 60810Poly-Gly

    Sequence similaritiesi

    Belongs to the DSH family.Curated
    Contains 1 DEP domain.PROSITE-ProRule annotation
    Contains 1 DIX domain.PROSITE-ProRule annotation
    Contains 1 PDZ (DHR) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG322275.
    GeneTreeiENSGT00390000013552.
    InParanoidiP51140.
    KOiK02353.
    OMAiIHQANQL.
    OrthoDBiEOG7BP82N.
    PhylomeDBiP51140.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.30.42.10. 1 hit.
    InterProiIPR000591. DEP_dom.
    IPR008339. Dishevelled_fam.
    IPR003351. Dishevelled_protein_dom.
    IPR001158. DIX.
    IPR026542. Dsh.
    IPR015506. Dsh/Dvl-rel.
    IPR001478. PDZ.
    IPR029071. Ubiquitin-rel_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR10878. PTHR10878. 1 hit.
    PTHR10878:SF19. PTHR10878:SF19. 1 hit.
    PfamiPF00610. DEP. 1 hit.
    PF02377. Dishevelled. 1 hit.
    PF00778. DIX. 1 hit.
    PF00595. PDZ. 1 hit.
    [Graphical view]
    PRINTSiPR01760. DISHEVELLED.
    SMARTiSM00021. DAX. 1 hit.
    SM00049. DEP. 1 hit.
    SM00228. PDZ. 1 hit.
    [Graphical view]
    SUPFAMiSSF50156. SSF50156. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEiPS50186. DEP. 1 hit.
    PS50841. DIX. 1 hit.
    PS50106. PDZ. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P51140-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDADRGGGQE TKVIYHIDDE TTPYLVKIPI PSAQVTLRDF KLVLNKQNNN    50
    YKYFFKSMDA DFGVVKEEIA DDSTILPCFN GRVVSWLVSA DGTNQSDNCS 100
    ELPTSECELG MGLTNRKLQQ QQQQHQQQQQ QQQQQHQQQQ QQQQQQVQPV 150
    QLAQQQQQQV LHHQKMMGNP LLQPPPLTYQ SASVLSSDLD STSLFGTESE 200
    LTLDRDMTDY SSVQRLQVRK KPQRRKKRAP SMSRTSSYSS ITDSTMSLNI 250
    ITVSINMEAV NFLGISIVGQ SNRGGDGGIY VGSIMKGGAV ALDGRIEPGD 300
    MILQVNDVNF ENMTNDEAVR VLREVVQKPG PIKLVVAKCW DPNPKGYFTI 350
    PRTEPVRPID PGAWVAHTQA LTSHDSIIAD IAEPIKERLD QNNLEEIVKA 400
    MTKPDSGLEI RDRMWLKITI PNAFIGADAV NWVLENVEDV QDRREARRIV 450
    SAMLRSNYIK HTVNKLTFSE QCYYVVNEER NPNLLGRGHL HPHQLPHGHG 500
    GHALSHADTE SITSDIGPLP NPPIYMPYSA TYNPSHGYQP IQYGIAERHI 550
    SSGSSSSDVL TSKDISASQS DITSVIHQAN QLTIAAHGSN KSSGSSNRGG 600
    GGGGGGGGNN TNDQDVSVFN YVL 623
    Length:623
    Mass (Da):68,917
    Last modified:June 21, 2005 - v2
    Checksum:i0BA24ED350666CF5
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti276 – 2761D → N in AAA20216. (PubMed:8149913)Curated
    Sequence conflicti565 – 5651I → T in AAA16535. (PubMed:8288125)Curated
    Sequence conflicti619 – 6191F → S in AAA16535. (PubMed:8288125)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02491 mRNA. Translation: AAA20216.1.
    L26974 mRNA. Translation: AAA16535.1.
    AE014298 Genomic DNA. Translation: AAF48033.1.
    BT023781 mRNA. Translation: AAZ41790.1.
    PIRiA49840.
    RefSeqiNP_511118.2. NM_078563.3.
    UniGeneiDm.2927.

    Genome annotation databases

    EnsemblMetazoaiFBtr0073454; FBpp0073310; FBgn0000499.
    GeneIDi32078.
    KEGGidme:Dmel_CG18361.
    UCSCiCG18361-RA. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U02491 mRNA. Translation: AAA20216.1 .
    L26974 mRNA. Translation: AAA16535.1 .
    AE014298 Genomic DNA. Translation: AAF48033.1 .
    BT023781 mRNA. Translation: AAZ41790.1 .
    PIRi A49840.
    RefSeqi NP_511118.2. NM_078563.3.
    UniGenei Dm.2927.

    3D structure databases

    ProteinModelPortali P51140.
    SMRi P51140. Positions 10-88, 250-338, 391-478.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 58490. 81 interactions.
    IntActi P51140. 16 interactions.
    MINTi MINT-8291181.

    Proteomic databases

    PaxDbi P51140.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0073454 ; FBpp0073310 ; FBgn0000499 .
    GeneIDi 32078.
    KEGGi dme:Dmel_CG18361.
    UCSCi CG18361-RA. d. melanogaster.

    Organism-specific databases

    CTDi 32078.
    FlyBasei FBgn0000499. dsh.

    Phylogenomic databases

    eggNOGi NOG322275.
    GeneTreei ENSGT00390000013552.
    InParanoidi P51140.
    KOi K02353.
    OMAi IHQANQL.
    OrthoDBi EOG7BP82N.
    PhylomeDBi P51140.

    Enzyme and pathway databases

    Reactomei REACT_207070. TCF dependent signaling in response to WNT.
    SignaLinki P51140.

    Miscellaneous databases

    GenomeRNAii 32078.
    NextBioi 776701.
    PROi P51140.

    Gene expression databases

    Bgeei P51140.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.30.42.10. 1 hit.
    InterProi IPR000591. DEP_dom.
    IPR008339. Dishevelled_fam.
    IPR003351. Dishevelled_protein_dom.
    IPR001158. DIX.
    IPR026542. Dsh.
    IPR015506. Dsh/Dvl-rel.
    IPR001478. PDZ.
    IPR029071. Ubiquitin-rel_dom.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10878. PTHR10878. 1 hit.
    PTHR10878:SF19. PTHR10878:SF19. 1 hit.
    Pfami PF00610. DEP. 1 hit.
    PF02377. Dishevelled. 1 hit.
    PF00778. DIX. 1 hit.
    PF00595. PDZ. 1 hit.
    [Graphical view ]
    PRINTSi PR01760. DISHEVELLED.
    SMARTi SM00021. DAX. 1 hit.
    SM00049. DEP. 1 hit.
    SM00228. PDZ. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50156. SSF50156. 1 hit.
    SSF54236. SSF54236. 1 hit.
    PROSITEi PS50186. DEP. 1 hit.
    PS50841. DIX. 1 hit.
    PS50106. PDZ. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Dishevelled is required during wingless signaling to establish both cell polarity and cell identity."
      Theisen H., Purcell J., Bennett M., Kansagara D., Syed A., Marsh J.L.
      Development 120:347-360(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "The Drosophila segment polarity gene dishevelled encodes a novel protein required for response to the wingless signal."
      Klingensmith J., Nusse R., Perrimon N.
      Genes Dev. 8:118-130(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Celniker S.E.
      Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    6. "The dishevelled protein is modified by wingless signaling in Drosophila."
      Yanagawa S., van Leeuwen F., Wodarz A., Klingensmith J., Nusse R.
      Genes Dev. 9:1087-1097(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
    7. "Naked cuticle targets dishevelled to antagonize Wnt signal transduction."
      Rousset R., Mack J.A., Wharton K.A. Jr., Axelrod J.D., Cadigan K.M., Fish M.P., Nusse R., Scott M.P.
      Genes Dev. 15:658-671(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NKD.
    8. "Zinc-dependent interaction between dishevelled and the Drosophila Wnt antagonist naked cuticle."
      Rousset R., Wharton K.A. Jr., Zimmermann G., Scott M.P.
      J. Biol. Chem. 277:49019-49026(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NKD.
    9. "An unconventional nuclear localization motif is crucial for function of the Drosophila Wnt/wingless antagonist Naked cuticle."
      Waldrop S., Chan C.-C., Cagatay T., Zhang S., Rousset R., Mack J.A., Zeng W., Fish M.P., Zhang M., Amanai M., Wharton K.A. Jr.
      Genetics 174:331-348(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NKD, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiDSH_DROME
    AccessioniPrimary (citable) accession number: P51140
    Secondary accession number(s): Q494J5, Q9VYZ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 137 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3