Reviewed, UniProtKB/Swiss-Prot P51135 (UCRI5_TOBAC)

Names and origin

Protein names	Recommended name:     Cytochrome b-c1 complex subunit Rieske-5, mitochondrial     EC=1.10.2.2 Alternative name(s):     Ubiquinol-cytochrome c reductase iron-sulfur subunit 5     Rieske iron-sulfur protein 5       Short name=RISP5     Complex III subunit 5-5
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	268 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The bc1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins.
Subcellular location	Mitochondrion inner membrane.
Tissue specificity	High levels are seen in the flowers while a low level expression is seen in the roots, leaves and stems.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 56	56	Mitochondrion Potential
	Chain	57 – 268	212	Cytochrome b-c1 complex subunit Rieske-5, mitochondrial		PRO_0000030680
Regions
	Domain	178 – 266	89	Rieske
Sites
	Metal binding	211	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	213	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	230	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	233	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	216 ↔ 232		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51135-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 464EC059ADE51F0F	FASTA	268	29,262
	10 20 30 40 50 60 MLRIAGRKLS SSAAARSSSA FFTRNPFTFT DDSSSPTRSP SPTSLASQFL DQFRGFSSNS 70 80 90 100 110 120 VSPAHQTGLV SDLPATVAAI KNPSSKIVYD DSNHERYPPG DPSKRAFAYF VLTGGRFVYA 130 140 150 160 170 180 SLVRLLILKF VLSMSASKDV LALASLEVDL SSIEPGTTVT VKWRGKPVFI RRRTDEDINL 190 200 210 220 230 240 ANSVDLGSLR DPQQDAERVK NPEWLVVIGV CTHLGCIPLP NAGDFGGWFC PCHGSHYDIS 250 260 GRIRKGPAPY NLEVPTYSFM EENKLLIG

References

[1]

"Flower-enhanced expression of a nuclear-encoded mitochondrial respiratory protein is associated with changes in mitochondrion number." Huang J., Struck F., Matzinger D.F., Levings C.S. III Plant Cell 6:439-448(1994) [PubMed: 8180500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. SC58. Tissue: Flower.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L16813 mRNA. Translation: AAA20834.1.
PIR	T02023.
3D structure databases
SMR	P51135. Positions 101-268.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.10.2.2. 298.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. IPR004192. Ubiquinol_cyt_Rdtase_TM. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF02921. UCR_TM. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI5_TOBAC

Accession

Primary (citable) accession number: P51135

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)