Reviewed, UniProtKB/Swiss-Prot P51134 (UCRI4_TOBAC)

Names and origin

Protein names	Recommended name:     Cytochrome b-c1 complex subunit Rieske-4, mitochondrial     EC=1.10.2.2 Alternative name(s):     Ubiquinol-cytochrome c reductase iron-sulfur subunit 4     Rieske iron-sulfur protein 4       Short name=RISP4     Complex III subunit 5-4
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.
Catalytic activity	QH2 + 2 ferricytochrome c = Q + 2 ferrocytochrome c + 2 H+.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The bc1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins.
Subcellular location	Mitochondrion inner membrane.
Miscellaneous	The Rieske protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Mitochondrion Potential
	Chain	25 – 236	212	Cytochrome b-c1 complex subunit Rieske-4, mitochondrial		PRO_0000030679
Regions
	Domain	146 – 234	89	Rieske
Sites
	Metal binding	179	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	181	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	198	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	201	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	184 ↔ 200		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P51134-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: AEDFC23B7EE40D0F	FASTA	236	25,822
	10 20 30 40 50 60 MINFGSCWGL ASVTSNSFSI ISGFSSNSVS HAHDMGLVPD LPPTVAAIKN PTSKIVYDEH 70 80 90 100 110 120 NHERYPPGDP SKRAFAYFVL TGGRFVYASL VRLLILKFVL SMSASKDVLA LASLEVDLSS 130 140 150 160 170 180 IEPGTTVTVK WRGKPVFIRR RTEDDINLAN SVDLGSLRDP QQDAERVKSP EWLVVIGVCT 190 200 210 220 230 HLGCIPLPNA GDFGGWFCPC HGSHYDISGR IRKGPAPYNL EVPTYSFLEE NKLLIG

References

[1]

"Flower-enhanced expression of a nuclear-encoded mitochondrial respiratory protein is associated with changes in mitochondrion number." Huang J., Struck F., Matzinger D.F., Levings C.S. III Plant Cell 6:439-448(1994) [PubMed: 8180500] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. SC58. Tissue: Flower.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L16812 mRNA. Translation: AAA20833.1.
PIR	T02020.
3D structure databases
SMR	P51134. Positions 69-236.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.10.2.2. 298.
Family and domain databases
InterPro	IPR017941. Rieske_2Fe-2S. IPR014349. Rieske_Fe-S_prot. IPR005805. Rieske_Fe-S_prot_C. IPR006317. Ubiquinol_cyt_c_Rdtase_Fe-S-su. IPR004192. Ubiquinol_cyt_Rdtase_TM. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF00355. Rieske. 1 hit. PF02921. UCR_TM. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01416. Rieske_proteo. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI4_TOBAC

Accession

Primary (citable) accession number: P51134

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)